LOCUS       sequence-345464-        5747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0902:Dendra
FEATURES             Location/Qualifiers
     source          1..5747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1202..1221
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1202..1220
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2847..2870
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3127..3927
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      4052..4640
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4541..4560
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagctctggt gcgtgcgtgg tgggccggga tgggtaggaa gcacccgact gcccgccggc
       61 atgcctacaa cgtcctccgg gcggtgatga acacagcggt cgaggacaag ctgatcgcag
      121 agaacccgtg ccggatcgag cagaaggcag ccgatgagcg cgacgtagag gcgctgacgc
      181 ctgaggagct ggacatcgtc gccgctgaga tcttcgagca ctaccggatc gcggcataca
      241 tcctggcgtg gacgagcctc cggttcggag agctgatcga gcttcgccgc aaggacatcg
      301 tggacgacgg catgacgatg aagctccggg tgcgccgtgg cgcttcccgc gtggggaaca
      361 agatcgtcgt tggcaacgcc aagaccgtcc ggtcgaagcg tcctgtgacg gttccgcctc
      421 acgtcgcgga gatgatccga gcgcacatga aggaccgtac gaagatgaac aagggccccg
      481 aggcattcct ggtgaccacg acgcagggca accggctgtc gaagtccgcg ttcaccaagt
      541 cgctgaagcg tggctacgcc aagatcggtc ggccggaact ccgcatccac gacctccgcg
      601 ctgtcggcgc tacgttcgcc gctcaggcag gtgcgacgac caaggagctg atggcccgtc
      661 tcggtcacac gactcctagg atggcgatga agtaccagat ggcgtctgag gcccgcgacg
      721 aggctatcgc tgaggcgatg tccaagctgg ccaagacctc ctgaaacgca aaaagccccc
      781 ctcccaagga cactgagtcc taaagagggg ggtttcttgt cagtacgcga agaaccacgc
      841 ctggccgcga gcgccagcac cgccgctctg tgcggagacc tgggcaccag ccccgccgcc
      901 gccaggagca ttgccgttcc cgccagaaat ctagacggtg accacaacgc gcccgctttg
      961 atcggggacg tcgaaatatt ggatcgtcgg caccgtcacg gccgtgggag gcggcacgat
     1021 ccgcgacgtg atgatcggcc gcatccccac ggtgctgcgc agtgagctct acgccatccc
     1081 ggcgttgatc tgtgcgttcg cacgcacagg cccggtgtga gaagggtctc tgacgagcgg
     1141 gagaactccc tatcagtgat agagtttgtc ctccctatca gtgatagata ggcttaagga
     1201 gtaatacgac tcactatagg gagaaggagg ccattatgcg cgagacgctc actcccaact
     1261 ttgccgatgt gcaggcccat tacgaccttt ccgacgactt cttccggttg ttcctggatc
     1321 cgacgcagac ctacagctgt gcgtacttcg cccaagacgg gatgacgctg gaagaggcac
     1381 agatcgcgaa gatcgacctg gctctgggca agctgggact ggagccgggg atgacactgc
     1441 tcgacgtggg ctgcggctgg ggtgcgacca tgcggcgcgc ggtcgaacgc cacgacgtca
     1501 acgtggtggg actgacactg tcgaggaacc aggccgccca cgtcgagcgc atgctcgacg
     1561 acgtgggcag caggcgttcc cggcgcgtgc tgctgcaggg gtgggagcag ttcgacgagc
     1621 ccgtcgaccg catcgtgtcg atcggcgcgt tcgagcactt ccggcacgag cgctacgacg
     1681 agttcttcgc caaggcccac gcgctgctgc ctgacgacgg cgtcatgctg ctgcacacca
     1741 tctgcgggtt ccaccccaac cactttcgcg aggcgggaat cccgatgacg atggagatgg
     1801 cgaagttcgt caagttcatc ctcaccgaga tcttccccgg cgggcagctg ccgtcatcgg
     1861 agatggtgca cgaccgcgcc ggacgggccg gcttcgtcgt gtcccgcgag caatcgctgc
     1921 agccgcacta cgccaagacc ctcgacatct gggcgtcgcg cctgcaggcg caccaggacg
     1981 aggccatcgc gatccagtcg ctggaggtct accagcggta catgaagtac ctgaccgggt
     2041 gcgcgcagct gttccgcgac ggccaggtgg acgtcgtgca gttcaccctg cagaaagggc
     2101 gcaagcttgg tgcggccgcg ggcaacacgc ctggcatcaa tctgatcaag gaggacatgc
     2161 gtgtcaaggt gcatatggag ggcaacgtga acggtcacgc cttcgtgatc gagggggagg
     2221 gtaagggtaa gccttacgaa gggacccaga ccgccaacct caccgtcaag gagggcgccc
     2281 ctctcccatt ctcgtacgat atcctcacta ccgcggtgca ttacgggaac cgcgtgttca
     2341 ccaagtaccc cgaggatatc cctgactact tcaagcagag cttcccggag ggatactcct
     2401 gggagcggac gatgaccttc gaagacaagg gtatctgtac gatccgcagc gacatctcgc
     2461 tggaagggga ctgtttcttc cagaacgtcc gtttcaaggg gaccaacttt ccgccgaacg
     2521 gcccggtcat gcagaagaag accctcaagt gggagccgag cacggagaag ctgcacgttc
     2581 gggacggcct cctggtcggc aacatcaaca tggcgctgct gctggaaggc ggcggccact
     2641 acctatgcga cttcaagacc acgtacaaag cgaagaaggt cgtccagctg cccgacgcgc
     2701 acttcgtgga ccaccgcatc gaaatcctgg ggaacgattc ggactacaac aaggtcaagt
     2761 tgtacgagca cgctgtggca cgctatagcc cgctcccgtc gcaggtctgg ttcgaaggcg
     2821 gcggcgggga gaacctgtat ttccaggact acaaggacga cgacgacaag tgatttaaat
     2881 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gttaactagc gtacgatcga
     2941 attcctgcag ctcacggtaa ctgatgccgt atttgcagta ccagcgtacg gcccacagaa
     3001 tgatgtcacg ctgaaaatgc cggcctttga atgggttcat gtgcagctcc atcagcaaaa
     3061 ggggatgata agtttatcac caccgactat ttgcaacagt gccgttgatc gtgctatgat
     3121 cgactgatgt catcagcggt ggagtgcaat gtcgtgcaat acgaatggcg aaaagccgag
     3181 ctcatcggtc agcttctcaa ccttggggtt acccccggcg gtgtgctgct ggtccacagc
     3241 tccttccgta gcgtccggcc cctcgaagat gggccacttg gactgatcga ggccctgcgt
     3301 gctacgctgg gtccgggagg gacgctcgtc atgccctcgt ggtcaggtct ggacgacgag
     3361 ccgttcgatc ctgccacgtc gcccgttaca ccggaccttg gagttgtctc tgacacattc
     3421 tggcgcctgc caaatgtaaa gcgcagcgcc catccatttg cctttgcggc agcggggcca
     3481 caggcagagc agatcatctc tgatccattg cccctgccac cttactcgcc tgcaagcccg
     3541 gtcgcccgtg tccatgaact cgatgggcag gtacttctcc tcggcgtggg acacgatgcc
     3601 aacacgacgc tgcatcttgc cgagttgatg gcaaaggttc cctatggggt gccgagacac
     3661 tgcaccattc ttcaggatgg caagttggta cgcgtcgatt atctcgagaa tgaccactgc
     3721 tgtgagcgct ttgccttggc ggacaggtgg ctcaaggaga agagccttca gaaggaaggt
     3781 ccagtcggtc atgcctttgc tcggttgatc cgctcccgcg acattgtggc gacagccctg
     3841 ggtcaactgg gccgagatcc gttgatcttc ctgcatccgc cagaggcggg atgcgaagaa
     3901 tgcgatgccg ctcgccagtc gattggctga gctcatgagc ggagaacgag atgacgttgg
     3961 aggggcaagg tcgcgctgat tgctggggca acacggggga tccactagtt ccatgagcgt
     4021 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4081 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4141 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     4201 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4261 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4321 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4381 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4441 agctatgaga aagcgccacg cttcccgagg ggagaaaggc ggacaggtat ccggtaagcg
     4501 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4561 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4621 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4681 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4741 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcaacgcgt
     4801 gcggccgcgg tacccgggga tcctctagag tcgaccacca agggcaccat ctctgcttgg
     4861 gccaccccgt tggccgcagc cagctcgctg agagccgtga acgacagggc gaacgccagc
     4921 ccgccgacgg cgagggttcc gaccgctgca actcccggtg caaccttgtc ccggtctatt
     4981 ctcttcactg caccagctcc aatctggtgt gaatgcccct cgtctgttcg cgcaggcggg
     5041 gggctctatt cgtttgtcag catcgaaagt agccagatca gggatgcgtt gcaaccgcgt
     5101 atgcccaggt cagaagagtc gcacaagagt tgcagacccc tggaaagaaa aatggccaga
     5161 gggcgaaaac accctctgac cagcggagcg ggcgacggga atcgaacccg cgtagctagt
     5221 ttggaagaat gggtgtctgc cgaccacata tgggccggtc aagataggtt tttaccccct
     5281 ctcggctgca tcctctaagt ggaaagaaat tgcaggtcgt agaagcgcgt tgaagcctga
     5341 gagttgcaca ggagttgcaa cccggtagcc ttgttcacga cgagaggaga cctagttggc
     5401 acgtcgcgga tggggatcgc tgaagactca gcgcagcggg aggatccaag cctcatacgt
     5461 caacccgcag gacggtgtga ggtactacgc gctgcagacc tacgacaaca agatggacgc
     5521 cgaagcctgg ctcgcgggcg agaagcggct catcgagatg gagacctgga cccctccaca
     5581 ggaccgggcg aagaaggcag ccgccagcgc catcacgctg gaggagtaca cccggaagtg
     5641 gctcgtggag cgcgacctcg cagacggcac cagggatctg tacagcgggc acgcggagcg
     5701 ccgcatctac ccggtgctag gtgaagtggc ggtcacagag atgacgc
//
