LOCUS       sequence-345465-        5561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0922:Dendra
FEATURES             Location/Qualifiers
     source          1..5561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     622..641
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        622..640
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2081..2104
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2361..3161
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3286..3874
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3775..3794
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgcatccac gacctccgcg ctgtcggcgc tacgttcgcc gctcaggcag gtgcgacgac
       61 caaggagctg atggcccgtc tcggtcacac gactcctagg atggcgatga agtaccagat
      121 ggcgtctgag gcccgcgacg aggctatcgc tgaggcgatg tccaagctgg ccaagacctc
      181 ctgaaacgca aaaagccccc ctcccaagga cactgagtcc taaagagggg ggtttcttgt
      241 cagtacgcga agaaccacgc ctggccgcga gcgccagcac cgccgctctg tgcggagacc
      301 tgggcaccag ccccgccgcc gccaggagca ttgccgttcc cgccagaaat ctagacggtg
      361 accacaacgc gcccgctttg atcggggacg tcgaaatatt ggatcgtcgg caccgtcacg
      421 gccgtgggag gcggcacgat ccgcgacgtg atgatcggcc gcatccccac ggtgctgcgc
      481 agtgagctct acgccatccc ggcgttgatc tgtgcgttcg cacgcacagg cccggtgtga
      541 gaagggtctc tgacgagcgg gagaactccc tatcagtgat agagtttgtc ctccctatca
      601 gtgatagata ggcttaagga gtaatacgac tcactatagg gagaaggagg ccattatgac
      661 cgaccacacc cgcgcacagg tcgcagcact cgtcgaccac accctgctca agccggaggc
      721 gacgccgtcc gacgtcaccg ctctcgtgga cgaggcggcc gacctgggag tcttcgcggt
      781 ctgcgtatcg ccgccgctgg tgtcggtcgc cgcaggcgtc gcgccgtcgg gcctggcgat
      841 cgccgcggtg gccgggttcc cgtccggcaa gcacgtgccc ggtatcaagg ccaccgaggc
      901 cgaactcgcg gtcgcggcag gcgccaccga gatcgacatg gtgatcgacg tcggtgcggc
      961 cctcgcgggg gatctcgacg ccgtgtccgc cgacatcacc gcggtccgca aggccgtgcg
     1021 cgcggcgacg ctcaaggtca tcgtggagtc ggcggcgctc ctggagttct cgggagaacc
     1081 gttgctggcc gatgtgtgcc gcgtcgcgcg cgatgcggga gccgacttcg tgaagacctc
     1141 cacgggtttc cacccgtccg gcggcgcgtc ggtacaggcg gtcgagatca tggcgcgcac
     1201 cgtgggcgag cgcctcgggg tcaaggccag cggcgggatc cgcaccgccg agcaggccgc
     1261 ggccatgctc gacgcgggag ccacccggct gggcctgtcc ggctcacgcg cggtgctcga
     1321 cgggttcggc tcggccaagc ttggtgcggc cgcgggcaac acgcctggca tcaatctgat
     1381 caaggaggac atgcgtgtca aggtgcatat ggagggcaac gtgaacggtc acgccttcgt
     1441 gatcgagggg gagggtaagg gtaagcctta cgaagggacc cagaccgcca acctcaccgt
     1501 caaggagggc gcccctctcc cattctcgta cgatatcctc actaccgcgg tgcattacgg
     1561 gaaccgcgtg ttcaccaagt accccgagga tatccctgac tacttcaagc agagcttccc
     1621 ggagggatac tcctgggagc ggacgatgac cttcgaagac aagggtatct gtacgatccg
     1681 cagcgacatc tcgctggaag gggactgttt cttccagaac gtccgtttca aggggaccaa
     1741 ctttccgccg aacggcccgg tcatgcagaa gaagaccctc aagtgggagc cgagcacgga
     1801 gaagctgcac gttcgggacg gcctcctggt cggcaacatc aacatggcgc tgctgctgga
     1861 aggcggcggc cactacctat gcgacttcaa gaccacgtac aaagcgaaga aggtcgtcca
     1921 gctgcccgac gcgcacttcg tggaccaccg catcgaaatc ctggggaacg attcggacta
     1981 caacaaggtc aagttgtacg agcacgctgt ggcacgctat agcccgctcc cgtcgcaggt
     2041 ctggttcgaa ggcggcggcg gggagaacct gtatttccag gactacaagg acgacgacga
     2101 caagtgattt aaataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgttaac
     2161 tagcgtacga tcgaattcct gcagctcacg gtaactgatg ccgtatttgc agtaccagcg
     2221 tacggcccac agaatgatgt cacgctgaaa atgccggcct ttgaatgggt tcatgtgcag
     2281 ctccatcagc aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgccgtt
     2341 gatcgtgcta tgatcgactg atgtcatcag cggtggagtg caatgtcgtg caatacgaat
     2401 ggcgaaaagc cgagctcatc ggtcagcttc tcaaccttgg ggttaccccc ggcggtgtgc
     2461 tgctggtcca cagctccttc cgtagcgtcc ggcccctcga agatgggcca cttggactga
     2521 tcgaggccct gcgtgctacg ctgggtccgg gagggacgct cgtcatgccc tcgtggtcag
     2581 gtctggacga cgagccgttc gatcctgcca cgtcgcccgt tacaccggac cttggagttg
     2641 tctctgacac attctggcgc ctgccaaatg taaagcgcag cgcccatcca tttgcctttg
     2701 cggcagcggg gccacaggca gagcagatca tctctgatcc attgcccctg ccaccttact
     2761 cgcctgcaag cccggtcgcc cgtgtccatg aactcgatgg gcaggtactt ctcctcggcg
     2821 tgggacacga tgccaacacg acgctgcatc ttgccgagtt gatggcaaag gttccctatg
     2881 gggtgccgag acactgcacc attcttcagg atggcaagtt ggtacgcgtc gattatctcg
     2941 agaatgacca ctgctgtgag cgctttgcct tggcggacag gtggctcaag gagaagagcc
     3001 ttcagaagga aggtccagtc ggtcatgcct ttgctcggtt gatccgctcc cgcgacattg
     3061 tggcgacagc cctgggtcaa ctgggccgag atccgttgat cttcctgcat ccgccagagg
     3121 cgggatgcga agaatgcgat gccgctcgcc agtcgattgg ctgagctcat gagcggagaa
     3181 cgagatgacg ttggaggggc aaggtcgcgc tgattgctgg ggcaacacgg gggatccact
     3241 agttccatga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3301 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3361 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3421 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3481 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3541 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3601 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3661 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaggggagaa aggcggacag
     3721 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3781 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3841 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3901 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3961 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4021 cgagcgcaac gcgtgcggcc gcggtacccg gggatcctct agagtcgacc accaagggca
     4081 ccatctctgc ttgggccacc ccgttggccg cagccagctc gctgagagcc gtgaacgaca
     4141 gggcgaacgc cagcccgccg acggcgaggg ttccgaccgc tgcaactccc ggtgcaacct
     4201 tgtcccggtc tattctcttc actgcaccag ctccaatctg gtgtgaatgc ccctcgtctg
     4261 ttcgcgcagg cggggggctc tattcgtttg tcagcatcga aagtagccag atcagggatg
     4321 cgttgcaacc gcgtatgccc aggtcagaag agtcgcacaa gagttgcaga cccctggaaa
     4381 gaaaaatggc cagagggcga aaacaccctc tgaccagcgg agcgggcgac gggaatcgaa
     4441 cccgcgtagc tagtttggaa gaatgggtgt ctgccgacca catatgggcc ggtcaagata
     4501 ggtttttacc ccctctcggc tgcatcctct aagtggaaag aaattgcagg tcgtagaagc
     4561 gcgttgaagc ctgagagttg cacaggagtt gcaacccggt agccttgttc acgacgagag
     4621 gagacctagt tggcacgtcg cggatgggga tcgctgaaga ctcagcgcag cgggaggatc
     4681 caagcctcat acgtcaaccc gcaggacggt gtgaggtact acgcgctgca gacctacgac
     4741 aacaagatgg acgccgaagc ctggctcgcg ggcgagaagc ggctcatcga gatggagacc
     4801 tggacccctc cacaggaccg ggcgaagaag gcagccgcca gcgccatcac gctggaggag
     4861 tacacccgga agtggctcgt ggagcgcgac ctcgcagacg gcaccaggga tctgtacagc
     4921 gggcacgcgg agcgccgcat ctacccggtg ctaggtgaag tggcggtcac agagatgacg
     4981 ccagctctgg tgcgtgcgtg gtgggccggg atgggtagga agcacccgac tgcccgccgg
     5041 catgcctaca acgtcctccg ggcggtgatg aacacagcgg tcgaggacaa gctgatcgca
     5101 gagaacccgt gccggatcga gcagaaggca gccgatgagc gcgacgtaga ggcgctgacg
     5161 cctgaggagc tggacatcgt cgccgctgag atcttcgagc actaccggat cgcggcatac
     5221 atcctggcgt ggacgagcct ccggttcgga gagctgatcg agcttcgccg caaggacatc
     5281 gtggacgacg gcatgacgat gaagctccgg gtgcgccgtg gcgcttcccg cgtggggaac
     5341 aagatcgtcg ttggcaacgc caagaccgtc cggtcgaagc gtcctgtgac ggttccgcct
     5401 cacgtcgcgg agatgatccg agcgcacatg aaggaccgta cgaagatgaa caagggcccc
     5461 gaggcattcc tggtgaccac gacgcagggc aaccggctgt cgaagtccgc gttcaccaag
     5521 tcgctgaagc gtggctacgc caagatcggt cggccggaac t
//
