LOCUS       sequence-345466-        5768 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0949:Dendra
FEATURES             Location/Qualifiers
     source          1..5768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     757..776
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1118..1129
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2423..2446
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2703..3503
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3628..4216
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4117..4136
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgaaggac cgtacgaaga tgaacaaggg ccccgaggca ttcctggtga ccacgacgca
       61 gggcaaccgg ctgtcgaagt ccgcgttcac caagtcgctg aagcgtggct acgccaagat
      121 cggtcggccg gaactccgca tccacgacct ccgcgctgtc ggcgctacgt tcgccgctca
      181 ggcaggtgcg acgaccaagg agctgatggc ccgtctcggt cacacgactc ctaggatggc
      241 gatgaagtac cagatggcgt ctgaggcccg cgacgaggct atcgctgagg cgatgtccaa
      301 gctggccaag acctcctgaa acgcaaaaag cccccctccc aaggacactg agtcctaaag
      361 aggggggttt cttgtcagta cgcgaagaac cacgcctggc cgcgagcgcc agcaccgccg
      421 ctctgtgcgg agacctgggc accagccccg ccgccgccag gagcattgcc gttcccgcca
      481 gaaatctaga cggtgaccac aacgcgcccg ctttgatcgg ggacgtcgaa atattggatc
      541 gtcggcaccg tcacggccgt gggaggcggc acgatccgcg acgtgatgat cggccgcatc
      601 cccacggtgc tgcgcagtga gctctacgcc atcccggcgt tgatctgtgc gttcgcacgc
      661 acaggcccgg tgtgagaagg gtctctgacg agcgggagaa ctccctatca gtgatagagt
      721 ttgtcctccc tatcagtgat agataggctt aaggagtaat acgactcact atagggagaa
      781 ggaggccatt atgactgatg aggccgggga ggctcgcggg gctcaggagc tcggcggcga
      841 ggccagtgcc gaggtggccg tcaccgacct gcaggccgag cagacacctc caccgagcgc
      901 tcctccgccg gacctgaccg ccgcggcgtt cttcgacgtc gacaacaccc tggtgcaagg
      961 ctcgtcgctg gtgcacttcg cgcgcggcct ggcggccagg aagtacttca cctaccgcga
     1021 catcctaggc atcgcatatg cgcaggccaa attccagctg accggcaagg agaacagcga
     1081 cgacgtcgcc gcgggccggc gcaaggcgct cgcattcatc gagggccggt cgaccgccga
     1141 actggtcgcg ctcggcgagg agatctacga cgagatcatc gccgacaaga tctggcccgg
     1201 cacccgcgcg ctggcccaga tgcacctcga cgccggtcag caggtctggc tggtcaccgc
     1261 gacgccctac gagctggcgg ccacgatcgc caagcggctg ggcttcacgg gcgcactggg
     1321 cacggtcgcg gagtcggtcg acggcgtctt caccgggcgt ctggtgggcg acatcctgca
     1381 cggcaccggc aaggcgcacg ccgtgcgctc gctggcgatc cgcgagggtc tgaacctgcg
     1441 ccgctgcacg gcctactccg acagcttcaa cgacgtgccc atgctgtcgc tcgtcggcac
     1501 cgcggtcgcg atcaacccgg acgccgcgct gcgcgacgtc gcgcgggaac gcggctggga
     1561 gatccgcgac ttccgcacgg cccgcaaagc ggcccgcatc ggtgtgccgt ccgcgctggc
     1621 cctaggggcg ctcggcggcg cgctggccgc ggtggcctcc cgccgccatg accttcgcaa
     1681 gcttggtgcg gccgcgggca acacgcctgg catcaatctg atcaaggagg acatgcgtgt
     1741 caaggtgcat atggagggca acgtgaacgg tcacgccttc gtgatcgagg gggagggtaa
     1801 gggtaagcct tacgaaggga cccagaccgc caacctcacc gtcaaggagg gcgcccctct
     1861 cccattctcg tacgatatcc tcactaccgc ggtgcattac gggaaccgcg tgttcaccaa
     1921 gtaccccgag gatatccctg actacttcaa gcagagcttc ccggagggat actcctggga
     1981 gcggacgatg accttcgaag acaagggtat ctgtacgatc cgcagcgaca tctcgctgga
     2041 aggggactgt ttcttccaga acgtccgttt caaggggacc aactttccgc cgaacggccc
     2101 ggtcatgcag aagaagaccc tcaagtggga gccgagcacg gagaagctgc acgttcggga
     2161 cggcctcctg gtcggcaaca tcaacatggc gctgctgctg gaaggcggcg gccactacct
     2221 atgcgacttc aagaccacgt acaaagcgaa gaaggtcgtc cagctgcccg acgcgcactt
     2281 cgtggaccac cgcatcgaaa tcctggggaa cgattcggac tacaacaagg tcaagttgta
     2341 cgagcacgct gtggcacgct atagcccgct cccgtcgcag gtctggttcg aaggcggcgg
     2401 cggggagaac ctgtatttcc aggactacaa ggacgacgac gacaagtgat ttaaataacc
     2461 ccttggggcc tctaaacggg tcttgagggg ttttttgtta actagcgtac gatcgaattc
     2521 ctgcagctca cggtaactga tgccgtattt gcagtaccag cgtacggccc acagaatgat
     2581 gtcacgctga aaatgccggc ctttgaatgg gttcatgtgc agctccatca gcaaaagggg
     2641 atgataagtt tatcaccacc gactatttgc aacagtgccg ttgatcgtgc tatgatcgac
     2701 tgatgtcatc agcggtggag tgcaatgtcg tgcaatacga atggcgaaaa gccgagctca
     2761 tcggtcagct tctcaacctt ggggttaccc ccggcggtgt gctgctggtc cacagctcct
     2821 tccgtagcgt ccggcccctc gaagatgggc cacttggact gatcgaggcc ctgcgtgcta
     2881 cgctgggtcc gggagggacg ctcgtcatgc cctcgtggtc aggtctggac gacgagccgt
     2941 tcgatcctgc cacgtcgccc gttacaccgg accttggagt tgtctctgac acattctggc
     3001 gcctgccaaa tgtaaagcgc agcgcccatc catttgcctt tgcggcagcg gggccacagg
     3061 cagagcagat catctctgat ccattgcccc tgccacctta ctcgcctgca agcccggtcg
     3121 cccgtgtcca tgaactcgat gggcaggtac ttctcctcgg cgtgggacac gatgccaaca
     3181 cgacgctgca tcttgccgag ttgatggcaa aggttcccta tggggtgccg agacactgca
     3241 ccattcttca ggatggcaag ttggtacgcg tcgattatct cgagaatgac cactgctgtg
     3301 agcgctttgc cttggcggac aggtggctca aggagaagag ccttcagaag gaaggtccag
     3361 tcggtcatgc ctttgctcgg ttgatccgct cccgcgacat tgtggcgaca gccctgggtc
     3421 aactgggccg agatccgttg atcttcctgc atccgccaga ggcgggatgc gaagaatgcg
     3481 atgccgctcg ccagtcgatt ggctgagctc atgagcggag aacgagatga cgttggaggg
     3541 gcaaggtcgc gctgattgct ggggcaacac gggggatcca ctagttccat gagcgtcaga
     3601 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3661 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3721 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     3781 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3841 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3901 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3961 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4021 atgagaaagc gccacgcttc ccgaggggag aaaggcggac aggtatccgg taagcggcag
     4081 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4141 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4201 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4261 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     4321 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca acgcgtgcgg
     4381 ccgcggtacc cggggatcct ctagagtcga ccaccaaggg caccatctct gcttgggcca
     4441 ccccgttggc cgcagccagc tcgctgagag ccgtgaacga cagggcgaac gccagcccgc
     4501 cgacggcgag ggttccgacc gctgcaactc ccggtgcaac cttgtcccgg tctattctct
     4561 tcactgcacc agctccaatc tggtgtgaat gcccctcgtc tgttcgcgca ggcggggggc
     4621 tctattcgtt tgtcagcatc gaaagtagcc agatcaggga tgcgttgcaa ccgcgtatgc
     4681 ccaggtcaga agagtcgcac aagagttgca gacccctgga aagaaaaatg gccagagggc
     4741 gaaaacaccc tctgaccagc ggagcgggcg acgggaatcg aacccgcgta gctagtttgg
     4801 aagaatgggt gtctgccgac cacatatggg ccggtcaaga taggttttta ccccctctcg
     4861 gctgcatcct ctaagtggaa agaaattgca ggtcgtagaa gcgcgttgaa gcctgagagt
     4921 tgcacaggag ttgcaacccg gtagccttgt tcacgacgag aggagaccta gttggcacgt
     4981 cgcggatggg gatcgctgaa gactcagcgc agcgggagga tccaagcctc atacgtcaac
     5041 ccgcaggacg gtgtgaggta ctacgcgctg cagacctacg acaacaagat ggacgccgaa
     5101 gcctggctcg cgggcgagaa gcggctcatc gagatggaga cctggacccc tccacaggac
     5161 cgggcgaaga aggcagccgc cagcgccatc acgctggagg agtacacccg gaagtggctc
     5221 gtggagcgcg acctcgcaga cggcaccagg gatctgtaca gcgggcacgc ggagcgccgc
     5281 atctacccgg tgctaggtga agtggcggtc acagagatga cgccagctct ggtgcgtgcg
     5341 tggtgggccg ggatgggtag gaagcacccg actgcccgcc ggcatgccta caacgtcctc
     5401 cgggcggtga tgaacacagc ggtcgaggac aagctgatcg cagagaaccc gtgccggatc
     5461 gagcagaagg cagccgatga gcgcgacgta gaggcgctga cgcctgagga gctggacatc
     5521 gtcgccgctg agatcttcga gcactaccgg atcgcggcat acatcctggc gtggacgagc
     5581 ctccggttcg gagagctgat cgagcttcgc cgcaaggaca tcgtggacga cggcatgacg
     5641 atgaagctcc gggtgcgccg tggcgcttcc cgcgtgggga acaagatcgt cgttggcaac
     5701 gccaagaccg tccggtcgaa gcgtcctgtg acggttccgc ctcacgtcgc ggagatgatc
     5761 cgagcgca
//
