LOCUS       sequence-345467-        5852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1033:Dendra
FEATURES             Location/Qualifiers
     source          1..5852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     726..745
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        726..744
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2476..2499
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2756..3556
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3681..4269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4170..4189
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccgaggcat tcctggtgac cacgacgcag ggcaaccggc tgtcgaagtc cgcgttcacc
       61 aagtcgctga agcgtggcta cgccaagatc ggtcggccgg aactccgcat ccacgacctc
      121 cgcgctgtcg gcgctacgtt cgccgctcag gcaggtgcga cgaccaagga gctgatggcc
      181 cgtctcggtc acacgactcc taggatggcg atgaagtacc agatggcgtc tgaggcccgc
      241 gacgaggcta tcgctgaggc gatgtccaag ctggccaaga cctcctgaaa cgcaaaaagc
      301 ccccctccca aggacactga gtcctaaaga ggggggtttc ttgtcagtac gcgaagaacc
      361 acgcctggcc gcgagcgcca gcaccgccgc tctgtgcgga gacctgggca ccagccccgc
      421 cgccgccagg agcattgccg ttcccgccag aaatctagac ggtgaccaca acgcgcccgc
      481 tttgatcggg gacgtcgaaa tattggatcg tcggcaccgt cacggccgtg ggaggcggca
      541 cgatccgcga cgtgatgatc ggccgcatcc ccacggtgct gcgcagtgag ctctacgcca
      601 tcccggcgtt gatctgtgcg ttcgcacgca caggcccggt gtgagaaggg tctctgacga
      661 gcgggagaac tccctatcag tgatagagtt tgtcctccct atcagtgata gataggctta
      721 aggagtaata cgactcacta tagggagaag gaggccatta tgtccgacgg aatcaagctg
      781 atcgaccgtg tctcagccat caactggaac cggctccagg atgagaagga cgccgaggtg
      841 tggcaccggc tcaccggcaa tttctggttg ccggagaagg tgccggtgtc caacgacatc
      901 cagtcctggc acaccctcac cgacaacgag aagcagctga caatgcgggt gttcaccggc
      961 ctgacgctgc tcgacaccat ccagggcacc gtcggcgcgg tcagcctgat ccctgacgcg
     1021 gtcaccccgc acgaggaggc cgtgctcacc aacatcgcgt tcatggagtc ggtgcacgcc
     1081 aagagctaca gcaacatctt ctcgacgctg tgctcgaccg ccgagatcga cgacgcgttc
     1141 cgctggtccg aggagaaccc gaacctgcag cgcaaggccg cgatcgtgat gcagtactac
     1201 cgcggcgacg agccgctcaa gcgcaaggtg gcctcgacgc tgctggagag cttcctgttc
     1261 tactcgggct tctacctgcc gatgtactgg tccagccgcg ccaagctgac caacaccgcc
     1321 gacatgatcc gcctgatcat ccgcgacgag gccgtgcacg gctactacat cggctacaag
     1381 taccagcgcg ggctcgccct cgtcgacgag gaaaagaagc aggagctcaa ggactacacc
     1441 tacgaactgc tcttcgagct ctacgacaac gaggtcgagt acacccagga cctctacgac
     1501 agcgtcggcc tgaccgagga cgtcaagaag ttcctgcgct acaacgccaa caaggccctg
     1561 atgaacctcg gctacgaggc gctgttcccg cgcgacgaga ccgacgtcaa cccggcgatc
     1621 ctctcggcgc tggcgcccaa cgccgacgag aaccacgact tcttctccgg ctcggggtcg
     1681 agctacgtga tcggcaaggc cgtcaacacc gaagacgagg actgggactt caagcttggt
     1741 gcggccgcgg gcaacacgcc tggcatcaat ctgatcaagg aggacatgcg tgtcaaggtg
     1801 catatggagg gcaacgtgaa cggtcacgcc ttcgtgatcg agggggaggg taagggtaag
     1861 ccttacgaag ggacccagac cgccaacctc accgtcaagg agggcgcccc tctcccattc
     1921 tcgtacgata tcctcactac cgcggtgcat tacgggaacc gcgtgttcac caagtacccc
     1981 gaggatatcc ctgactactt caagcagagc ttcccggagg gatactcctg ggagcggacg
     2041 atgaccttcg aagacaaggg tatctgtacg atccgcagcg acatctcgct ggaaggggac
     2101 tgtttcttcc agaacgtccg tttcaagggg accaactttc cgccgaacgg cccggtcatg
     2161 cagaagaaga ccctcaagtg ggagccgagc acggagaagc tgcacgttcg ggacggcctc
     2221 ctggtcggca acatcaacat ggcgctgctg ctggaaggcg gcggccacta cctatgcgac
     2281 ttcaagacca cgtacaaagc gaagaaggtc gtccagctgc ccgacgcgca cttcgtggac
     2341 caccgcatcg aaatcctggg gaacgattcg gactacaaca aggtcaagtt gtacgagcac
     2401 gctgtggcac gctatagccc gctcccgtcg caggtctggt tcgaaggcgg cggcggggag
     2461 aacctgtatt tccaggacta caaggacgac gacgacaagt gatttaaata accccttggg
     2521 gcctctaaac gggtcttgag gggttttttg ttaactagcg tacgatcgaa ttcctgcagc
     2581 tcacggtaac tgatgccgta tttgcagtac cagcgtacgg cccacagaat gatgtcacgc
     2641 tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca tcagcaaaag gggatgataa
     2701 gtttatcacc accgactatt tgcaacagtg ccgttgatcg tgctatgatc gactgatgtc
     2761 atcagcggtg gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc tcatcggtca
     2821 gcttctcaac cttggggtta cccccggcgg tgtgctgctg gtccacagct ccttccgtag
     2881 cgtccggccc ctcgaagatg ggccacttgg actgatcgag gccctgcgtg ctacgctggg
     2941 tccgggaggg acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc cgttcgatcc
     3001 tgccacgtcg cccgttacac cggaccttgg agttgtctct gacacattct ggcgcctgcc
     3061 aaatgtaaag cgcagcgccc atccatttgc ctttgcggca gcggggccac aggcagagca
     3121 gatcatctct gatccattgc ccctgccacc ttactcgcct gcaagcccgg tcgcccgtgt
     3181 ccatgaactc gatgggcagg tacttctcct cggcgtggga cacgatgcca acacgacgct
     3241 gcatcttgcc gagttgatgg caaaggttcc ctatggggtg ccgagacact gcaccattct
     3301 tcaggatggc aagttggtac gcgtcgatta tctcgagaat gaccactgct gtgagcgctt
     3361 tgccttggcg gacaggtggc tcaaggagaa gagccttcag aaggaaggtc cagtcggtca
     3421 tgcctttgct cggttgatcc gctcccgcga cattgtggcg acagccctgg gtcaactggg
     3481 ccgagatccg ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat gcgatgccgc
     3541 tcgccagtcg attggctgag ctcatgagcg gagaacgaga tgacgttgga ggggcaaggt
     3601 cgcgctgatt gctggggcaa cacgggggat ccactagttc catgagcgtc agaccccgta
     3661 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3721 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3781 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     3841 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3901 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3961 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     4021 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     4081 agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     4141 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     4201 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     4261 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     4321 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     4381 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg cggccgcggt
     4441 acccggggat cctctagagt cgaccaccaa gggcaccatc tctgcttggg ccaccccgtt
     4501 ggccgcagcc agctcgctga gagccgtgaa cgacagggcg aacgccagcc cgccgacggc
     4561 gagggttccg accgctgcaa ctcccggtgc aaccttgtcc cggtctattc tcttcactgc
     4621 accagctcca atctggtgtg aatgcccctc gtctgttcgc gcaggcgggg ggctctattc
     4681 gtttgtcagc atcgaaagta gccagatcag ggatgcgttg caaccgcgta tgcccaggtc
     4741 agaagagtcg cacaagagtt gcagacccct ggaaagaaaa atggccagag ggcgaaaaca
     4801 ccctctgacc agcggagcgg gcgacgggaa tcgaacccgc gtagctagtt tggaagaatg
     4861 ggtgtctgcc gaccacatat gggccggtca agataggttt ttaccccctc tcggctgcat
     4921 cctctaagtg gaaagaaatt gcaggtcgta gaagcgcgtt gaagcctgag agttgcacag
     4981 gagttgcaac ccggtagcct tgttcacgac gagaggagac ctagttggca cgtcgcggat
     5041 ggggatcgct gaagactcag cgcagcggga ggatccaagc ctcatacgtc aacccgcagg
     5101 acggtgtgag gtactacgcg ctgcagacct acgacaacaa gatggacgcc gaagcctggc
     5161 tcgcgggcga gaagcggctc atcgagatgg agacctggac ccctccacag gaccgggcga
     5221 agaaggcagc cgccagcgcc atcacgctgg aggagtacac ccggaagtgg ctcgtggagc
     5281 gcgacctcgc agacggcacc agggatctgt acagcgggca cgcggagcgc cgcatctacc
     5341 cggtgctagg tgaagtggcg gtcacagaga tgacgccagc tctggtgcgt gcgtggtggg
     5401 ccgggatggg taggaagcac ccgactgccc gccggcatgc ctacaacgtc ctccgggcgg
     5461 tgatgaacac agcggtcgag gacaagctga tcgcagagaa cccgtgccgg atcgagcaga
     5521 aggcagccga tgagcgcgac gtagaggcgc tgacgcctga ggagctggac atcgtcgccg
     5581 ctgagatctt cgagcactac cggatcgcgg catacatcct ggcgtggacg agcctccggt
     5641 tcggagagct gatcgagctt cgccgcaagg acatcgtgga cgacggcatg acgatgaagc
     5701 tccgggtgcg ccgtggcgct tcccgcgtgg ggaacaagat cgtcgttggc aacgccaaga
     5761 ccgtccggtc gaagcgtcct gtgacggttc cgcctcacgt cgcggagatg atccgagcgc
     5821 acatgaagga ccgtacgaag atgaacaagg gc
//
