LOCUS       sequence-345470-        5369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1110:Dendra
FEATURES             Location/Qualifiers
     source          1..5369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1218..1237
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1218..1236
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2485..2508
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2765..3565
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3690..4278
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4179..4198
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcacagaga tgacgccagc tctggtgcgt gcgtggtggg ccgggatggg taggaagcac
       61 ccgactgccc gccggcatgc ctacaacgtc ctccgggcgg tgatgaacac agcggtcgag
      121 gacaagctga tcgcagagaa cccgtgccgg atcgagcaga aggcagccga tgagcgcgac
      181 gtagaggcgc tgacgcctga ggagctggac atcgtcgccg ctgagatctt cgagcactac
      241 cggatcgcgg catacatcct ggcgtggacg agcctccggt tcggagagct gatcgagctt
      301 cgccgcaagg acatcgtgga cgacggcatg acgatgaagc tccgggtgcg ccgtggcgct
      361 tcccgcgtgg ggaacaagat cgtcgttggc aacgccaaga ccgtccggtc gaagcgtcct
      421 gtgacggttc cgcctcacgt cgcggagatg atccgagcgc acatgaagga ccgtacgaag
      481 atgaacaagg gccccgaggc attcctggtg accacgacgc agggcaaccg gctgtcgaag
      541 tccgcgttca ccaagtcgct gaagcgtggc tacgccaaga tcggtcggcc ggaactccgc
      601 atccacgacc tccgcgctgt cggcgctacg ttcgccgctc aggcaggtgc gacgaccaag
      661 gagctgatgg cccgtctcgg tcacacgact cctaggatgg cgatgaagta ccagatggcg
      721 tctgaggccc gcgacgaggc tatcgctgag gcgatgtcca agctggccaa gacctcctga
      781 aacgcaaaaa gcccccctcc caaggacact gagtcctaaa gaggggggtt tcttgtcagt
      841 acgcgaagaa ccacgcctgg ccgcgagcgc cagcaccgcc gctctgtgcg gagacctggg
      901 caccagcccc gccgccgcca ggagcattgc cgttcccgcc agaaatctag acggtgacca
      961 caacgcgccc gctttgatcg gggacgtcga aatattggat cgtcggcacc gtcacggccg
     1021 tgggaggcgg cacgatccgc gacgtgatga tcggccgcat ccccacggtg ctgcgcagtg
     1081 agctctacgc catcccggcg ttgatctgtg cgttcgcacg cacaggcccg gtgtgagaag
     1141 ggtctctgac gagcgggaga actccctatc agtgatagag tttgtcctcc ctatcagtga
     1201 tagataggct taaggagtaa tacgactcac tatagggaga aggaggccat tatgcccgtc
     1261 gccgaccggg tgcgggccgc ggcccgcgtc gtgctcccgc atctctacgg cgagccgggg
     1321 gagacccgca ggcagcgcat catccggcgt gtgcgcatcg gcatcgtgat cgcggcgtgc
     1381 ctggtgacgc tgcagtcggt gctgttggtg ctcggcgcgt ggcgcaacga ccggcagatc
     1441 gaacgcaaca tgggtgtcgc cgcggccgag gtgctcagcg cgggcccgcg ccggtcgacc
     1501 atcgagttcg tcacgcccga ccgcgtcacc taccggcccg aactcggcgt gctgtacccc
     1561 agcgaactcg acaccggcat gcgcatctac gtcgagtacg acaagaacaa ccccgagctg
     1621 gtgcgcgtgc gcgaccgcaa cgccgcgctc gcgatcatcc cggccgggtc gatcgcggtg
     1681 gtggggtgga tgatcgcagg cgccgcactg gtcgggctgt ggctgttgca gcggcggacg
     1741 aagcttggtg cggccgcggg caacacgcct ggcatcaatc tgatcaagga ggacatgcgt
     1801 gtcaaggtgc atatggaggg caacgtgaac ggtcacgcct tcgtgatcga gggggagggt
     1861 aagggtaagc cttacgaagg gacccagacc gccaacctca ccgtcaagga gggcgcccct
     1921 ctcccattct cgtacgatat cctcactacc gcggtgcatt acgggaaccg cgtgttcacc
     1981 aagtaccccg aggatatccc tgactacttc aagcagagct tcccggaggg atactcctgg
     2041 gagcggacga tgaccttcga agacaagggt atctgtacga tccgcagcga catctcgctg
     2101 gaaggggact gtttcttcca gaacgtccgt ttcaagggga ccaactttcc gccgaacggc
     2161 ccggtcatgc agaagaagac cctcaagtgg gagccgagca cggagaagct gcacgttcgg
     2221 gacggcctcc tggtcggcaa catcaacatg gcgctgctgc tggaaggcgg cggccactac
     2281 ctatgcgact tcaagaccac gtacaaagcg aagaaggtcg tccagctgcc cgacgcgcac
     2341 ttcgtggacc accgcatcga aatcctgggg aacgattcgg actacaacaa ggtcaagttg
     2401 tacgagcacg ctgtggcacg ctatagcccg ctcccgtcgc aggtctggtt cgaaggcggc
     2461 ggcggggaga acctgtattt ccaggactac aaggacgacg acgacaagtg atttaaataa
     2521 ccccttgggg cctctaaacg ggtcttgagg ggttttttgt taactagcgt acgatcgaat
     2581 tcctgcagct cacggtaact gatgccgtat ttgcagtacc agcgtacggc ccacagaatg
     2641 atgtcacgct gaaaatgccg gcctttgaat gggttcatgt gcagctccat cagcaaaagg
     2701 ggatgataag tttatcacca ccgactattt gcaacagtgc cgttgatcgt gctatgatcg
     2761 actgatgtca tcagcggtgg agtgcaatgt cgtgcaatac gaatggcgaa aagccgagct
     2821 catcggtcag cttctcaacc ttggggttac ccccggcggt gtgctgctgg tccacagctc
     2881 cttccgtagc gtccggcccc tcgaagatgg gccacttgga ctgatcgagg ccctgcgtgc
     2941 tacgctgggt ccgggaggga cgctcgtcat gccctcgtgg tcaggtctgg acgacgagcc
     3001 gttcgatcct gccacgtcgc ccgttacacc ggaccttgga gttgtctctg acacattctg
     3061 gcgcctgcca aatgtaaagc gcagcgccca tccatttgcc tttgcggcag cggggccaca
     3121 ggcagagcag atcatctctg atccattgcc cctgccacct tactcgcctg caagcccggt
     3181 cgcccgtgtc catgaactcg atgggcaggt acttctcctc ggcgtgggac acgatgccaa
     3241 cacgacgctg catcttgccg agttgatggc aaaggttccc tatggggtgc cgagacactg
     3301 caccattctt caggatggca agttggtacg cgtcgattat ctcgagaatg accactgctg
     3361 tgagcgcttt gccttggcgg acaggtggct caaggagaag agccttcaga aggaaggtcc
     3421 agtcggtcat gcctttgctc ggttgatccg ctcccgcgac attgtggcga cagccctggg
     3481 tcaactgggc cgagatccgt tgatcttcct gcatccgcca gaggcgggat gcgaagaatg
     3541 cgatgccgct cgccagtcga ttggctgagc tcatgagcgg agaacgagat gacgttggag
     3601 gggcaaggtc gcgctgattg ctggggcaac acgggggatc cactagttcc atgagcgtca
     3661 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3721 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3781 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     3841 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3901 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3961 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4021 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4081 ctatgagaaa gcgccacgct tcccgagggg agaaaggcgg acaggtatcc ggtaagcggc
     4141 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4201 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4261 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4321 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4381 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg caacgcgtgc
     4441 ggccgcggta cccggggatc ctctagagtc gaccaccaag ggcaccatct ctgcttgggc
     4501 caccccgttg gccgcagcca gctcgctgag agccgtgaac gacagggcga acgccagccc
     4561 gccgacggcg agggttccga ccgctgcaac tcccggtgca accttgtccc ggtctattct
     4621 cttcactgca ccagctccaa tctggtgtga atgcccctcg tctgttcgcg caggcggggg
     4681 gctctattcg tttgtcagca tcgaaagtag ccagatcagg gatgcgttgc aaccgcgtat
     4741 gcccaggtca gaagagtcgc acaagagttg cagacccctg gaaagaaaaa tggccagagg
     4801 gcgaaaacac cctctgacca gcggagcggg cgacgggaat cgaacccgcg tagctagttt
     4861 ggaagaatgg gtgtctgccg accacatatg ggccggtcaa gataggtttt taccccctct
     4921 cggctgcatc ctctaagtgg aaagaaattg caggtcgtag aagcgcgttg aagcctgaga
     4981 gttgcacagg agttgcaacc cggtagcctt gttcacgacg agaggagacc tagttggcac
     5041 gtcgcggatg gggatcgctg aagactcagc gcagcgggag gatccaagcc tcatacgtca
     5101 acccgcagga cggtgtgagg tactacgcgc tgcagaccta cgacaacaag atggacgccg
     5161 aagcctggct cgcgggcgag aagcggctca tcgagatgga gacctggacc cctccacagg
     5221 accgggcgaa gaaggcagcc gccagcgcca tcacgctgga ggagtacacc cggaagtggc
     5281 tcgtggagcg cgacctcgca gacggcacca gggatctgta cagcgggcac gcggagcgcc
     5341 gcatctaccc ggtgctaggt gaagtggcg
//
