LOCUS       sequence-345471-        6797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1229:Dendra
FEATURES             Location/Qualifiers
     source          1..6797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1217..1236
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1217..1235
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3912..3935
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4192..4992
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      5117..5705
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5606..5625
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcacagagat gacgccagct ctggtgcgtg cgtggtgggc cgggatgggt aggaagcacc
       61 cgactgcccg ccggcatgcc tacaacgtcc tccgggcggt gatgaacaca gcggtcgagg
      121 acaagctgat cgcagagaac ccgtgccgga tcgagcagaa ggcagccgat gagcgcgacg
      181 tagaggcgct gacgcctgag gagctggaca tcgtcgccgc tgagatcttc gagcactacc
      241 ggatcgcggc atacatcctg gcgtggacga gcctccggtt cggagagctg atcgagcttc
      301 gccgcaagga catcgtggac gacggcatga cgatgaagct ccgggtgcgc cgtggcgctt
      361 cccgcgtggg gaacaagatc gtcgttggca acgccaagac cgtccggtcg aagcgtcctg
      421 tgacggttcc gcctcacgtc gcggagatga tccgagcgca catgaaggac cgtacgaaga
      481 tgaacaaggg ccccgaggca ttcctggtga ccacgacgca gggcaaccgg ctgtcgaagt
      541 ccgcgttcac caagtcgctg aagcgtggct acgccaagat cggtcggccg gaactccgca
      601 tccacgacct ccgcgctgtc ggcgctacgt tcgccgctca ggcaggtgcg acgaccaagg
      661 agctgatggc ccgtctcggt cacacgactc ctaggatggc gatgaagtac cagatggcgt
      721 ctgaggcccg cgacgaggct atcgctgagg cgatgtccaa gctggccaag acctcctgaa
      781 acgcaaaaag cccccctccc aaggacactg agtcctaaag aggggggttt cttgtcagta
      841 cgcgaagaac cacgcctggc cgcgagcgcc agcaccgccg ctctgtgcgg agacctgggc
      901 accagccccg ccgccgccag gagcattgcc gttcccgcca gaaatctaga cggtgaccac
      961 aacgcgcccg ctttgatcgg ggacgtcgaa atattggatc gtcggcaccg tcacggccgt
     1021 gggaggcggc acgatccgcg acgtgatgat cggccgcatc cccacggtgc tgcgcagtga
     1081 gctctacgcc atcccggcgt tgatctgtgc gttcgcacgc acaggcccgg tgtgagaagg
     1141 gtctctgacg agcgggagaa ctccctatca gtgatagagt ttgtcctccc tatcagtgat
     1201 agataggctt aaggagtaat acgactcact atagggagaa ggaggccatt atgtccacca
     1261 ccaactatga cgccggatcc atcacgatcc tcgaaggact cgacgtcgtc cggaaacgcc
     1321 cgggcatgta catcggctcc accagcgagc gcggcctgca ccacatggtg tgggaggtcg
     1381 tcgacaacgg cgtcgacgag gccatggccg gccacgcgac ccgcgtcgat gtcaccctgc
     1441 tcgccgacgg cggcgtgcag gtgtgcgacg acggccgcgg catccccgtc gcgatgcacg
     1501 aaaccggcat ccccacagtc gatgtggtga tgacccagct gcacgccggc ggcaagttcg
     1561 acagcgagtc ctaccaggtg tcgggcggtc tgcacggcgt cggggtctcc gtcgtcaacg
     1621 cgctgtctac ccgcttggag gtcgacatcc accgcgacgg ctacgaatgg caccagcact
     1681 acgaccactc cgtgcccgcc accctgaccc gcggcaaccc atccggcggc accggcacca
     1741 ccgttcggtt ctgggccgat cccgacgtgt tcgagaccac cacctacagc gccgagacca
     1801 tcgcgcggcg cctgcaggag atggcgttcc tcaacaaggg cctgatcctc acgctcaccg
     1861 atcaacgcga ccccgaaccg cagacgcgga cgttccacca ccccggcgga ttgatcgact
     1921 atgtcaagca catcaaccgg gtcaaagacc cgatccagcc cagcatcatc gcgttcgagg
     1981 gggaggggcc ggggcacgag gtcgagatcg cgatgcagtg gaacgcgggg tattcggagt
     2041 cggtgcacac cttcgccaac accatcaaca cgcatgaggg tgggacgcac gaggagggtt
     2101 tccgtgcggc gctgaccagc gtggtgaacc ggtatgccaa ggacaagaag ctgctcaagg
     2161 acaaggatcc caacctcacc ggtgatgaca tccgtgaggg tttggccgcc gtgatctcgg
     2221 tgaaggtggc cgaaccgcag ttcgagggcc agacgaaaac caagctgggt aacgcggcga
     2281 tccgctcgtt tgtgcagaag gtgtgccacg aacagctgac ccactggctc gaagcgaatc
     2341 ccgttgaggc caaggtgatc atcaccaagg tcgcgtcgtc ggctcaggcg cggatcgcgg
     2401 cgcgtaaggc gcgtgagttg gtgcggcgta agagcgcgac ggacatcggt gggttgccgg
     2461 gcaagctggc cgactgccgt tcgacggatc cgtccaagtc cgagctgtat gtggtggagg
     2521 gtgattccgc gggtggttcg gccaagagtg gccgggattc gatgttccag gcgatcctgc
     2581 cgttgcgagg caagatcatc aacgtcgaga aggcccggat cgaccgggtg ctgaagaaca
     2641 ccgaggtgca gtcgatcatc accgcgctgg gcaccggtat ccatgacgag ttcgacatct
     2701 ccaagctgcg gtatcacaag atcgtgttga tggccgatgc ggatgtggac ggtcagcaca
     2761 tctcgacgtt gttgttgacg ttgttgttcc ggttcatgaa gccgttggtg gaaaacggcc
     2821 acatcttctt ggcgcagccg ccgttgtaca agctcaagtg gcagcgcagt gagccggagt
     2881 tcgcgtattc cgatcgtgag cgtgacgggc tgttggaggc cgggcgcgcg gcgggcaaga
     2941 agatcaacgt cgacgacggt atccagcgct acaagggtct gggcgagatg gacgccaagg
     3001 agttgtggga gaccacgatg gatccgtcgg tgcgggtgct gcgacaggtc accctcgacg
     3061 atgctgccgc ggccgacgaa ctgttctcca tcctcatggg cgacgacgtg gaggcgcgac
     3121 gagggtttat cacccgcaac gccaaagaca ttcgcttcct cgacatcaag cttggtgcgg
     3181 ccgcgggcaa cacgcctggc atcaatctga tcaaggagga catgcgtgtc aaggtgcata
     3241 tggagggcaa cgtgaacggt cacgccttcg tgatcgaggg ggagggtaag ggtaagcctt
     3301 acgaagggac ccagaccgcc aacctcaccg tcaaggaggg cgcccctctc ccattctcgt
     3361 acgatatcct cactaccgcg gtgcattacg ggaaccgcgt gttcaccaag taccccgagg
     3421 atatccctga ctacttcaag cagagcttcc cggagggata ctcctgggag cggacgatga
     3481 ccttcgaaga caagggtatc tgtacgatcc gcagcgacat ctcgctggaa ggggactgtt
     3541 tcttccagaa cgtccgtttc aaggggacca actttccgcc gaacggcccg gtcatgcaga
     3601 agaagaccct caagtgggag ccgagcacgg agaagctgca cgttcgggac ggcctcctgg
     3661 tcggcaacat caacatggcg ctgctgctgg aaggcggcgg ccactaccta tgcgacttca
     3721 agaccacgta caaagcgaag aaggtcgtcc agctgcccga cgcgcacttc gtggaccacc
     3781 gcatcgaaat cctggggaac gattcggact acaacaaggt caagttgtac gagcacgctg
     3841 tggcacgcta tagcccgctc ccgtcgcagg tctggttcga aggcggcggc ggggagaacc
     3901 tgtatttcca ggactacaag gacgacgacg acaagtgatt taaataaccc cttggggcct
     3961 ctaaacgggt cttgaggggt tttttgttaa ctagcgtacg atcgaattcc tgcagctcac
     4021 ggtaactgat gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa
     4081 aatgccggcc tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt
     4141 atcaccaccg actatttgca acagtgccgt tgatcgtgct atgatcgact gatgtcatca
     4201 gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat cggtcagctt
     4261 ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc acagctcctt ccgtagcgtc
     4321 cggcccctcg aagatgggcc acttggactg atcgaggccc tgcgtgctac gctgggtccg
     4381 ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt cgatcctgcc
     4441 acgtcgcccg ttacaccgga ccttggagtt gtctctgaca cattctggcg cctgccaaat
     4501 gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg ggccacaggc agagcagatc
     4561 atctctgatc cattgcccct gccaccttac tcgcctgcaa gcccggtcgc ccgtgtccat
     4621 gaactcgatg ggcaggtact tctcctcggc gtgggacacg atgccaacac gacgctgcat
     4681 cttgccgagt tgatggcaaa ggttccctat ggggtgccga gacactgcac cattcttcag
     4741 gatggcaagt tggtacgcgt cgattatctc gagaatgacc actgctgtga gcgctttgcc
     4801 ttggcggaca ggtggctcaa ggagaagagc cttcagaagg aaggtccagt cggtcatgcc
     4861 tttgctcggt tgatccgctc ccgcgacatt gtggcgacag ccctgggtca actgggccga
     4921 gatccgttga tcttcctgca tccgccagag gcgggatgcg aagaatgcga tgccgctcgc
     4981 cagtcgattg gctgagctca tgagcggaga acgagatgac gttggagggg caaggtcgcg
     5041 ctgattgctg gggcaacacg ggggatccac tagttccatg agcgtcagac cccgtagaaa
     5101 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5161 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5221 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     5281 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5341 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5401 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     5461 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     5521 ccacgcttcc cgaggggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     5581 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     5641 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     5701 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     5761 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     5821 gagctgatac cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc cgcggtaccc
     5881 ggggatcctc tagagtcgac caccaagggc accatctctg cttgggccac cccgttggcc
     5941 gcagccagct cgctgagagc cgtgaacgac agggcgaacg ccagcccgcc gacggcgagg
     6001 gttccgaccg ctgcaactcc cggtgcaacc ttgtcccggt ctattctctt cactgcacca
     6061 gctccaatct ggtgtgaatg cccctcgtct gttcgcgcag gcggggggct ctattcgttt
     6121 gtcagcatcg aaagtagcca gatcagggat gcgttgcaac cgcgtatgcc caggtcagaa
     6181 gagtcgcaca agagttgcag acccctggaa agaaaaatgg ccagagggcg aaaacaccct
     6241 ctgaccagcg gagcgggcga cgggaatcga acccgcgtag ctagtttgga agaatgggtg
     6301 tctgccgacc acatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc
     6361 taagtggaaa gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt
     6421 tgcaacccgg tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg
     6481 atcgctgaag actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg
     6541 tgtgaggtac tacgcgctgc agacctacga caacaagatg gacgccgaag cctggctcgc
     6601 gggcgagaag cggctcatcg agatggagac ctggacccct ccacaggacc gggcgaagaa
     6661 ggcagccgcc agcgccatca cgctggagga gtacacccgg aagtggctcg tggagcgcga
     6721 cctcgcagac ggcaccaggg atctgtacag cgggcacgcg gagcgccgca tctacccggt
     6781 gctaggtgaa gtggcgg
//
