LOCUS       sequence-345473-        7160 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 7160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0786:Dendra
FEATURES             Location/Qualifiers
     source          1..7160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     715..734
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        715..733
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3773..3796
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4053..4853
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      4978..5566
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5467..5486
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctggtgacc acgacgcagg gcaaccggct gtcgaagtcc gcgttcacca agtcgctgaa
       61 gcgtggctac gccaagatcg gtcggccgga actccgcatc cacgacctcc gcgctgtcgg
      121 cgctacgttc gccgctcagg caggtgcgac gaccaaggag ctgatggccc gtctcggtca
      181 cacgactcct aggatggcga tgaagtacca gatggcgtct gaggcccgcg acgaggctat
      241 cgctgaggcg atgtccaagc tggccaagac ctcctgaaac gcaaaaagcc cccctcccaa
      301 ggacactgag tcctaaagag gggggtttct tgtcagtacg cgaagaacca cgcctggccg
      361 cgagcgccag caccgccgct ctgtgcggag acctgggcac cagccccgcc gccgccagga
      421 gcattgccgt tcccgccaga aatctagacg gtgaccacaa cgcgcccgct ttgatcgggg
      481 acgtcgaaat attggatcgt cggcaccgtc acggccgtgg gaggcggcac gatccgcgac
      541 gtgatgatcg gccgcatccc cacggtgctg cgcagtgagc tctacgccat cccggcgttg
      601 atctgtgcgt tcgcacgcac aggcccggtg tgagaagggt ctctgacgag cgggagaact
      661 ccctatcagt gatagagttt gtcctcccta tcagtgatag ataggcttaa ggagtaatac
      721 gactcactat agggagaagg aggccattat gacttcaccc gagaaccctg atctcccaga
      781 cgccgacgac gcttacgtcg acagcgggcc gggcacacaa ccggcgagcc tggaagacct
      841 cgacatggac tcggcgtcga ccatgcggcc catggcgacg caggccgtgt accggcccga
      901 gttcgacgac accgacggca catcgcgcgg cacggtggtc accgaggcct acgaccaggt
      961 caccatggcc acgcgcgcgt tgtccccgat gcggcgcctc ggcggcgggc tggtggagat
     1021 cccgcgtgtc cccgaacgcg acccgctcac cgcgttgatg accaatccgg ttgtggccga
     1081 gtcgaaacgg ttctgctgga actgcggcaa gcccgtcgga cggtcgacgc cagacggccg
     1141 cgccctgtcc gagggctggt gcccgcactg cggcagcccg tattcgttcc tgccgcaact
     1201 gtccccgggc gacatcgtgg ccgaccagta cgagatcaag ggttgcatcg cgcacggcgg
     1261 cctgggctgg gtgtacctgg cgttcgacaa gaacgtcaac gaccgcccgg tggtgctcaa
     1321 gggtctggtg cattccggtg acgccgaggc gcaggccatc gccatggccg aacggcagtt
     1381 cctcgccgag gtgacgcacc cgggaatcgt caagatctac aacttcgtcg agcacgagga
     1441 caagcacggc aaccccgtcg gctacatcgt gatggagtac gtcggcggca cgtcgctcaa
     1501 acaagccagg ggcgccaagc tcccggtggc cgaggcgatc ggctacatgc tcgagatcct
     1561 gcctgcgctg ggctatctgc attcgatcgg cctggcctac aacgacctca agcccgagaa
     1621 catcatgatc accgaggaac agctcaagct gatcgacctc ggtgcggtgt cgcgcctgaa
     1681 ctcctacgga tacctctacg gcacaccggg ttaccaggct cccgagatcg tgcgcaccgg
     1741 cccgacggtg gccaccgaca tctacaccgt gggccgcaca ctggccgcct tgacgctgtc
     1801 gctgcggacc cgacgtggac gctacgtcga cggcctgccg tccgacgatc ctgtgctgga
     1861 gacctacgac tcgtaccacc ggttgctgcg tcgcgcgatc gacccggatc cgcggcgacg
     1921 cttcaccagc gccgaggaga tgtcgtcgca gcttctcggt gtgctgcgcg aggtggtcgc
     1981 gaccgacacc ggggttccgc ggcccggcct gtcgacggtg ttcagcccat cacgctcgac
     2041 gttcggagtg gacctgctgg tggcgcacac cgacgtttac gttgacgggc aggtgcactc
     2101 ggagaagctg accgcgcagg agatcgtgcg ggcgctgccg gtgccgctgg tcgaccgcac
     2161 cgacgtcggc gcgccgatgc tggtggccag cgtgctcagc gagcccgtcc acacgctgga
     2221 ccagctgcgt gcggcgcgtc acggtgcgct cgacaccgag ggcatcgacc tcaacgagtc
     2281 ggtcgagctt ccgctcatgg aggtccgggc gctgctcgat ctcggtgatg tcgccaaggc
     2341 cacccgcaag ctcgaggatc tcgcggcgcg cgtcggttgg cgctggcggc tggtctggtt
     2401 caaggccgtc tccgagatgc tctcggccga ttacgattcg gcgacaaagc atttcaccga
     2461 agtgctggac acgctgcccg gtgagctggc gcccaagctc gcactcgccg cgaccgcgga
     2521 gttggcgggc acggccgacg agttgaagtt ctacaagacc gtgtggagca ccgacaacgg
     2581 cgtgatctcc gccgggttcg gcctggcccg cgcgcaatcg gtcgccggtg aacgcgatat
     2641 ggccgtgcag acgctcgacg aagttccgcc cacctcaagg catttcacca ctgcccggct
     2701 caccagcgcg gtgacactgc tgtcgggccg gtcgaccagt gagatcaccg agcagcacat
     2761 ccgcgacgcc gcacgccgcg tcgaggccct gccagactcc gaaccccgcg tgttgcagat
     2821 ccgcgcgctg gtgctgggaa ccgcgctcga ctggctcgcc gacaacaccg cgagcagcaa
     2881 ccacattctg ggattcccgt tcaccgagca cggtctcaag ctcggcgtcg aggcatcgct
     2941 gcgcgcactg gcccgcatcg ctcccacgca gtcgcaccgc tacgccctgg tcgacctggc
     3001 caacagcgtg cggccgatga gcacgttcaa gcttggtgcg gccgcgggca acacgcctgg
     3061 catcaatctg atcaaggagg acatgcgtgt caaggtgcat atggagggca acgtgaacgg
     3121 tcacgccttc gtgatcgagg gggagggtaa gggtaagcct tacgaaggga cccagaccgc
     3181 caacctcacc gtcaaggagg gcgcccctct cccattctcg tacgatatcc tcactaccgc
     3241 ggtgcattac gggaaccgcg tgttcaccaa gtaccccgag gatatccctg actacttcaa
     3301 gcagagcttc ccggagggat actcctggga gcggacgatg accttcgaag acaagggtat
     3361 ctgtacgatc cgcagcgaca tctcgctgga aggggactgt ttcttccaga acgtccgttt
     3421 caaggggacc aactttccgc cgaacggccc ggtcatgcag aagaagaccc tcaagtggga
     3481 gccgagcacg gagaagctgc acgttcggga cggcctcctg gtcggcaaca tcaacatggc
     3541 gctgctgctg gaaggcggcg gccactacct atgcgacttc aagaccacgt acaaagcgaa
     3601 gaaggtcgtc cagctgcccg acgcgcactt cgtggaccac cgcatcgaaa tcctggggaa
     3661 cgattcggac tacaacaagg tcaagttgta cgagcacgct gtggcacgct atagcccgct
     3721 cccgtcgcag gtctggttcg aaggcggcgg cggggagaac ctgtatttcc aggactacaa
     3781 ggacgacgac gacaagtgat ttaaataacc ccttggggcc tctaaacggg tcttgagggg
     3841 ttttttgtta actagcgtac gatcgaattc ctgcagctca cggtaactga tgccgtattt
     3901 gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc ctttgaatgg
     3961 gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcaccacc gactatttgc
     4021 aacagtgccg ttgatcgtgc tatgatcgac tgatgtcatc agcggtggag tgcaatgtcg
     4081 tgcaatacga atggcgaaaa gccgagctca tcggtcagct tctcaacctt ggggttaccc
     4141 ccggcggtgt gctgctggtc cacagctcct tccgtagcgt ccggcccctc gaagatgggc
     4201 cacttggact gatcgaggcc ctgcgtgcta cgctgggtcc gggagggacg ctcgtcatgc
     4261 cctcgtggtc aggtctggac gacgagccgt tcgatcctgc cacgtcgccc gttacaccgg
     4321 accttggagt tgtctctgac acattctggc gcctgccaaa tgtaaagcgc agcgcccatc
     4381 catttgcctt tgcggcagcg gggccacagg cagagcagat catctctgat ccattgcccc
     4441 tgccacctta ctcgcctgca agcccggtcg cccgtgtcca tgaactcgat gggcaggtac
     4501 ttctcctcgg cgtgggacac gatgccaaca cgacgctgca tcttgccgag ttgatggcaa
     4561 aggttcccta tggggtgccg agacactgca ccattcttca ggatggcaag ttggtacgcg
     4621 tcgattatct cgagaatgac cactgctgtg agcgctttgc cttggcggac aggtggctca
     4681 aggagaagag ccttcagaag gaaggtccag tcggtcatgc ctttgctcgg ttgatccgct
     4741 cccgcgacat tgtggcgaca gccctgggtc aactgggccg agatccgttg atcttcctgc
     4801 atccgccaga ggcgggatgc gaagaatgcg atgccgctcg ccagtcgatt ggctgagctc
     4861 atgagcggag aacgagatga cgttggaggg gcaaggtcgc gctgattgct ggggcaacac
     4921 gggggatcca ctagttccat gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4981 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5041 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5101 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     5161 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5221 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5281 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5341 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaggggag
     5401 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5461 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5521 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5581 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5641 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     5701 cagccgaacg accgagcgca acgcgtgcgg ccgcggtacc cggggatcct ctagagtcga
     5761 ccaccaaggg caccatctct gcttgggcca ccccgttggc cgcagccagc tcgctgagag
     5821 ccgtgaacga cagggcgaac gccagcccgc cgacggcgag ggttccgacc gctgcaactc
     5881 ccggtgcaac cttgtcccgg tctattctct tcactgcacc agctccaatc tggtgtgaat
     5941 gcccctcgtc tgttcgcgca ggcggggggc tctattcgtt tgtcagcatc gaaagtagcc
     6001 agatcaggga tgcgttgcaa ccgcgtatgc ccaggtcaga agagtcgcac aagagttgca
     6061 gacccctgga aagaaaaatg gccagagggc gaaaacaccc tctgaccagc ggagcgggcg
     6121 acgggaatcg aacccgcgta gctagtttgg aagaatgggt gtctgccgac cacatatggg
     6181 ccggtcaaga taggttttta ccccctctcg gctgcatcct ctaagtggaa agaaattgca
     6241 ggtcgtagaa gcgcgttgaa gcctgagagt tgcacaggag ttgcaacccg gtagccttgt
     6301 tcacgacgag aggagaccta gttggcacgt cgcggatggg gatcgctgaa gactcagcgc
     6361 agcgggagga tccaagcctc atacgtcaac ccgcaggacg gtgtgaggta ctacgcgctg
     6421 cagacctacg acaacaagat ggacgccgaa gcctggctcg cgggcgagaa gcggctcatc
     6481 gagatggaga cctggacccc tccacaggac cgggcgaaga aggcagccgc cagcgccatc
     6541 acgctggagg agtacacccg gaagtggctc gtggagcgcg acctcgcaga cggcaccagg
     6601 gatctgtaca gcgggcacgc ggagcgccgc atctacccgg tgctaggtga agtggcggtc
     6661 acagagatga cgccagctct ggtgcgtgcg tggtgggccg ggatgggtag gaagcacccg
     6721 actgcccgcc ggcatgccta caacgtcctc cgggcggtga tgaacacagc ggtcgaggac
     6781 aagctgatcg cagagaaccc gtgccggatc gagcagaagg cagccgatga gcgcgacgta
     6841 gaggcgctga cgcctgagga gctggacatc gtcgccgctg agatcttcga gcactaccgg
     6901 atcgcggcat acatcctggc gtggacgagc ctccggttcg gagagctgat cgagcttcgc
     6961 cgcaaggaca tcgtggacga cggcatgacg atgaagctcc gggtgcgccg tggcgcttcc
     7021 cgcgtgggga acaagatcgt cgttggcaac gccaagaccg tccggtcgaa gcgtcctgtg
     7081 acggttccgc ctcacgtcgc ggagatgatc cgagcgcaca tgaaggaccg tacgaagatg
     7141 aacaagggcc ccgaggcatt
//
