LOCUS       sequence-345474-        5189 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0833:Dendra
FEATURES             Location/Qualifiers
     source          1..5189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     757..776
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1844..1867
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2124..2924
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3049..3637
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3538..3557
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgaaggac cgtacgaaga tgaacaaggg ccccgaggca ttcctggtga ccacgacgca
       61 gggcaaccgg ctgtcgaagt ccgcgttcac caagtcgctg aagcgtggct acgccaagat
      121 cggtcggccg gaactccgca tccacgacct ccgcgctgtc ggcgctacgt tcgccgctca
      181 ggcaggtgcg acgaccaagg agctgatggc ccgtctcggt cacacgactc ctaggatggc
      241 gatgaagtac cagatggcgt ctgaggcccg cgacgaggct atcgctgagg cgatgtccaa
      301 gctggccaag acctcctgaa acgcaaaaag cccccctccc aaggacactg agtcctaaag
      361 aggggggttt cttgtcagta cgcgaagaac cacgcctggc cgcgagcgcc agcaccgccg
      421 ctctgtgcgg agacctgggc accagccccg ccgccgccag gagcattgcc gttcccgcca
      481 gaaatctaga cggtgaccac aacgcgcccg ctttgatcgg ggacgtcgaa atattggatc
      541 gtcggcaccg tcacggccgt gggaggcggc acgatccgcg acgtgatgat cggccgcatc
      601 cccacggtgc tgcgcagtga gctctacgcc atcccggcgt tgatctgtgc gttcgcacgc
      661 acaggcccgg tgtgagaagg gtctctgacg agcgggagaa ctccctatca gtgatagagt
      721 ttgtcctccc tatcagtgat agataggctt aaggagtaat acgactcact atagggagaa
      781 ggaggccatt atggacggcg ccatggcgcg gactgagcaa tccggtgacg actctgatct
      841 gaccgatggg cttacccggc gcgaacacga cattttggcg ttcgagcgcc agtggtggaa
      901 gtacgcgggc agcaaggaag acgcgatcaa agagctgttc tcgatgtcgg ccacccggta
      961 ttaccaggtg ctgaacgcac tggtggaccg gcccgaggcc ctcgctgccg accccatgct
     1021 ggtcaaacgt ctgcgccggt tgcgtgccag ccggcagaag gcccgtgccg cgcgccggct
     1081 gggcttcgac atcagcaaga agcttggtgc ggccgcgggc aacacgcctg gcatcaatct
     1141 gatcaaggag gacatgcgtg tcaaggtgca tatggagggc aacgtgaacg gtcacgcctt
     1201 cgtgatcgag ggggagggta agggtaagcc ttacgaaggg acccagaccg ccaacctcac
     1261 cgtcaaggag ggcgcccctc tcccattctc gtacgatatc ctcactaccg cggtgcatta
     1321 cgggaaccgc gtgttcacca agtaccccga ggatatccct gactacttca agcagagctt
     1381 cccggaggga tactcctggg agcggacgat gaccttcgaa gacaagggta tctgtacgat
     1441 ccgcagcgac atctcgctgg aaggggactg tttcttccag aacgtccgtt tcaaggggac
     1501 caactttccg ccgaacggcc cggtcatgca gaagaagacc ctcaagtggg agccgagcac
     1561 ggagaagctg cacgttcggg acggcctcct ggtcggcaac atcaacatgg cgctgctgct
     1621 ggaaggcggc ggccactacc tatgcgactt caagaccacg tacaaagcga agaaggtcgt
     1681 ccagctgccc gacgcgcact tcgtggacca ccgcatcgaa atcctgggga acgattcgga
     1741 ctacaacaag gtcaagttgt acgagcacgc tgtggcacgc tatagcccgc tcccgtcgca
     1801 ggtctggttc gaaggcggcg gcggggagaa cctgtatttc caggactaca aggacgacga
     1861 cgacaagtga tttaaataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt
     1921 aactagcgta cgatcgaatt cctgcagctc acggtaactg atgccgtatt tgcagtacca
     1981 gcgtacggcc cacagaatga tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg
     2041 cagctccatc agcaaaaggg gatgataagt ttatcaccac cgactatttg caacagtgcc
     2101 gttgatcgtg ctatgatcga ctgatgtcat cagcggtgga gtgcaatgtc gtgcaatacg
     2161 aatggcgaaa agccgagctc atcggtcagc ttctcaacct tggggttacc cccggcggtg
     2221 tgctgctggt ccacagctcc ttccgtagcg tccggcccct cgaagatggg ccacttggac
     2281 tgatcgaggc cctgcgtgct acgctgggtc cgggagggac gctcgtcatg ccctcgtggt
     2341 caggtctgga cgacgagccg ttcgatcctg ccacgtcgcc cgttacaccg gaccttggag
     2401 ttgtctctga cacattctgg cgcctgccaa atgtaaagcg cagcgcccat ccatttgcct
     2461 ttgcggcagc ggggccacag gcagagcaga tcatctctga tccattgccc ctgccacctt
     2521 actcgcctgc aagcccggtc gcccgtgtcc atgaactcga tgggcaggta cttctcctcg
     2581 gcgtgggaca cgatgccaac acgacgctgc atcttgccga gttgatggca aaggttccct
     2641 atggggtgcc gagacactgc accattcttc aggatggcaa gttggtacgc gtcgattatc
     2701 tcgagaatga ccactgctgt gagcgctttg ccttggcgga caggtggctc aaggagaaga
     2761 gccttcagaa ggaaggtcca gtcggtcatg cctttgctcg gttgatccgc tcccgcgaca
     2821 ttgtggcgac agccctgggt caactgggcc gagatccgtt gatcttcctg catccgccag
     2881 aggcgggatg cgaagaatgc gatgccgctc gccagtcgat tggctgagct catgagcgga
     2941 gaacgagatg acgttggagg ggcaaggtcg cgctgattgc tggggcaaca cgggggatcc
     3001 actagttcca tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3061 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3121 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3181 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3241 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3301 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3361 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3421 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgagggga gaaaggcgga
     3481 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3541 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3601 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3661 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     3721 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3781 gaccgagcgc aacgcgtgcg gccgcggtac ccggggatcc tctagagtcg accaccaagg
     3841 gcaccatctc tgcttgggcc accccgttgg ccgcagccag ctcgctgaga gccgtgaacg
     3901 acagggcgaa cgccagcccg ccgacggcga gggttccgac cgctgcaact cccggtgcaa
     3961 ccttgtcccg gtctattctc ttcactgcac cagctccaat ctggtgtgaa tgcccctcgt
     4021 ctgttcgcgc aggcgggggg ctctattcgt ttgtcagcat cgaaagtagc cagatcaggg
     4081 atgcgttgca accgcgtatg cccaggtcag aagagtcgca caagagttgc agacccctgg
     4141 aaagaaaaat ggccagaggg cgaaaacacc ctctgaccag cggagcgggc gacgggaatc
     4201 gaacccgcgt agctagtttg gaagaatggg tgtctgccga ccacatatgg gccggtcaag
     4261 ataggttttt accccctctc ggctgcatcc tctaagtgga aagaaattgc aggtcgtaga
     4321 agcgcgttga agcctgagag ttgcacagga gttgcaaccc ggtagccttg ttcacgacga
     4381 gaggagacct agttggcacg tcgcggatgg ggatcgctga agactcagcg cagcgggagg
     4441 atccaagcct catacgtcaa cccgcaggac ggtgtgaggt actacgcgct gcagacctac
     4501 gacaacaaga tggacgccga agcctggctc gcgggcgaga agcggctcat cgagatggag
     4561 acctggaccc ctccacagga ccgggcgaag aaggcagccg ccagcgccat cacgctggag
     4621 gagtacaccc ggaagtggct cgtggagcgc gacctcgcag acggcaccag ggatctgtac
     4681 agcgggcacg cggagcgccg catctacccg gtgctaggtg aagtggcggt cacagagatg
     4741 acgccagctc tggtgcgtgc gtggtgggcc gggatgggta ggaagcaccc gactgcccgc
     4801 cggcatgcct acaacgtcct ccgggcggtg atgaacacag cggtcgagga caagctgatc
     4861 gcagagaacc cgtgccggat cgagcagaag gcagccgatg agcgcgacgt agaggcgctg
     4921 acgcctgagg agctggacat cgtcgccgct gagatcttcg agcactaccg gatcgcggca
     4981 tacatcctgg cgtggacgag cctccggttc ggagagctga tcgagcttcg ccgcaaggac
     5041 atcgtggacg acggcatgac gatgaagctc cgggtgcgcc gtggcgcttc ccgcgtgggg
     5101 aacaagatcg tcgttggcaa cgccaagacc gtccggtcga agcgtcctgt gacggttccg
     5161 cctcacgtcg cggagatgat ccgagcgca
//
