LOCUS       sequence-345475-        6272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0903:Dendra
FEATURES             Location/Qualifiers
     source          1..6272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     685..704
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        685..703
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2855..2878
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3135..3935
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      4060..4648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4549..4568
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcgaagtcc gcgttcacca agtcgctgaa gcgtggctac gccaagatcg gtcggccgga
       61 actccgcatc cacgacctcc gcgctgtcgg cgctacgttc gccgctcagg caggtgcgac
      121 gaccaaggag ctgatggccc gtctcggtca cacgactcct aggatggcga tgaagtacca
      181 gatggcgtct gaggcccgcg acgaggctat cgctgaggcg atgtccaagc tggccaagac
      241 ctcctgaaac gcaaaaagcc cccctcccaa ggacactgag tcctaaagag gggggtttct
      301 tgtcagtacg cgaagaacca cgcctggccg cgagcgccag caccgccgct ctgtgcggag
      361 acctgggcac cagccccgcc gccgccagga gcattgccgt tcccgccaga aatctagacg
      421 gtgaccacaa cgcgcccgct ttgatcgggg acgtcgaaat attggatcgt cggcaccgtc
      481 acggccgtgg gaggcggcac gatccgcgac gtgatgatcg gccgcatccc cacggtgctg
      541 cgcagtgagc tctacgccat cccggcgttg atctgtgcgt tcgcacgcac aggcccggtg
      601 tgagaagggt ctctgacgag cgggagaact ccctatcagt gatagagttt gtcctcccta
      661 tcagtgatag ataggcttaa ggagtaatac gactcactat agggagaagg aggccattat
      721 gacccacttt gacgttgttg ttctcggagc cggtcccggt ggatacgtcg cggccattcg
      781 cgccgcccag ctggggctga ataccgcaat tgtcgaaccc aagtactggg gcggcgtgtg
      841 tctcaacgtt ggctgtatcc cgtccaaggc gctgctgcgg aacgccgaga tcgcccacat
      901 gttcaacaag gaagccaaga ccttcggcat cagtggtgag gcgacgttcg actacggcgc
      961 ggcgttcgac cgcagccgca aggtcgccga cggccgtgtc gcgggcgtgc acttcctgat
     1021 gaagaagaac aagatcaccg agatccacgg ttacgggaag ttcaccggcc ccaacagcct
     1081 ggaggtcgcg ctcaacgacg gcgggaccga gaaagtcgag ttctccaacg cgatcatcgc
     1141 gaccggcagc agcacgcgcc tggtgcccgg cacgtcactg tcggagaacg tggtcaccta
     1201 cgagacacag atcctctcgc gtgagctgcc cggctcgatc atcatcgccg gcgccggcgc
     1261 gatcggcatg gagttcgcgt acgtgctgaa gaactacggc gtcgacgtca ccatcgtcga
     1321 gttcctgccg cgcgccctgc ccaacgagga cgccgaggtc tccaaggaga tcgagaagca
     1381 gtacaagaag ctgggcgtga agatcctcac cggcaccaag gtggagggta ttgccgatga
     1441 cggctcgacg gtgaaggtga ccgtcagcaa ggacggcaag accgaggaac tcaaggccga
     1501 caaggtgctg caggccatcg gcttcgcacc caacgtcgag ggttacggcc tggaggcggc
     1561 cggtgtcgag ctcaccgacc gcaaggccat cggcatcgac gactacatgc gtaccaacgt
     1621 ggggcacatc tacgccatcg gtgacgtcac cggcaagctt cagctcgccc acgtcgccga
     1681 ggcgatggcg gtggtcgcgg ccgaaaccat cgccggcgca gagactctgc cgctcggcga
     1741 ctaccgcatg atgccgcgcg cgacgttttg ccagccgcag gtcgcgagct tcgggctcac
     1801 cgaggagcag gcccgcgacg agggttacga cgtgaaggtc gccaagttcc cgttcaccgc
     1861 gaacggcaag gcgcacggcc tggccgaccc gaccgggttc gtgaagctca tcgccgacgc
     1921 caagtacggc gaactgctcg gcgggcacct catcggcccc gacgtctcgg agttgctgcc
     1981 tgagctgacc ctggcccaga agtgggacct gaccgtcaac gagctcgccc gcaacgtgca
     2041 cacccacccg acgctgtcgg aggcgttgca ggagtgcttc cacggtctgg ccggccacat
     2101 gatcaacttt aagcttggtg cggccgcggg caacacgcct ggcatcaatc tgatcaagga
     2161 ggacatgcgt gtcaaggtgc atatggaggg caacgtgaac ggtcacgcct tcgtgatcga
     2221 gggggagggt aagggtaagc cttacgaagg gacccagacc gccaacctca ccgtcaagga
     2281 gggcgcccct ctcccattct cgtacgatat cctcactacc gcggtgcatt acgggaaccg
     2341 cgtgttcacc aagtaccccg aggatatccc tgactacttc aagcagagct tcccggaggg
     2401 atactcctgg gagcggacga tgaccttcga agacaagggt atctgtacga tccgcagcga
     2461 catctcgctg gaaggggact gtttcttcca gaacgtccgt ttcaagggga ccaactttcc
     2521 gccgaacggc ccggtcatgc agaagaagac cctcaagtgg gagccgagca cggagaagct
     2581 gcacgttcgg gacggcctcc tggtcggcaa catcaacatg gcgctgctgc tggaaggcgg
     2641 cggccactac ctatgcgact tcaagaccac gtacaaagcg aagaaggtcg tccagctgcc
     2701 cgacgcgcac ttcgtggacc accgcatcga aatcctgggg aacgattcgg actacaacaa
     2761 ggtcaagttg tacgagcacg ctgtggcacg ctatagcccg ctcccgtcgc aggtctggtt
     2821 cgaaggcggc ggcggggaga acctgtattt ccaggactac aaggacgacg acgacaagtg
     2881 atttaaataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgt taactagcgt
     2941 acgatcgaat tcctgcagct cacggtaact gatgccgtat ttgcagtacc agcgtacggc
     3001 ccacagaatg atgtcacgct gaaaatgccg gcctttgaat gggttcatgt gcagctccat
     3061 cagcaaaagg ggatgataag tttatcacca ccgactattt gcaacagtgc cgttgatcgt
     3121 gctatgatcg actgatgtca tcagcggtgg agtgcaatgt cgtgcaatac gaatggcgaa
     3181 aagccgagct catcggtcag cttctcaacc ttggggttac ccccggcggt gtgctgctgg
     3241 tccacagctc cttccgtagc gtccggcccc tcgaagatgg gccacttgga ctgatcgagg
     3301 ccctgcgtgc tacgctgggt ccgggaggga cgctcgtcat gccctcgtgg tcaggtctgg
     3361 acgacgagcc gttcgatcct gccacgtcgc ccgttacacc ggaccttgga gttgtctctg
     3421 acacattctg gcgcctgcca aatgtaaagc gcagcgccca tccatttgcc tttgcggcag
     3481 cggggccaca ggcagagcag atcatctctg atccattgcc cctgccacct tactcgcctg
     3541 caagcccggt cgcccgtgtc catgaactcg atgggcaggt acttctcctc ggcgtgggac
     3601 acgatgccaa cacgacgctg catcttgccg agttgatggc aaaggttccc tatggggtgc
     3661 cgagacactg caccattctt caggatggca agttggtacg cgtcgattat ctcgagaatg
     3721 accactgctg tgagcgcttt gccttggcgg acaggtggct caaggagaag agccttcaga
     3781 aggaaggtcc agtcggtcat gcctttgctc ggttgatccg ctcccgcgac attgtggcga
     3841 cagccctggg tcaactgggc cgagatccgt tgatcttcct gcatccgcca gaggcgggat
     3901 gcgaagaatg cgatgccgct cgccagtcga ttggctgagc tcatgagcgg agaacgagat
     3961 gacgttggag gggcaaggtc gcgctgattg ctggggcaac acgggggatc cactagttcc
     4021 atgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4081 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4141 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4201 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4261 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4321 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4381 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4441 acagcgtgag ctatgagaaa gcgccacgct tcccgagggg agaaaggcgg acaggtatcc
     4501 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4561 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4621 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4681 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     4741 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     4801 caacgcgtgc ggccgcggta cccggggatc ctctagagtc gaccaccaag ggcaccatct
     4861 ctgcttgggc caccccgttg gccgcagcca gctcgctgag agccgtgaac gacagggcga
     4921 acgccagccc gccgacggcg agggttccga ccgctgcaac tcccggtgca accttgtccc
     4981 ggtctattct cttcactgca ccagctccaa tctggtgtga atgcccctcg tctgttcgcg
     5041 caggcggggg gctctattcg tttgtcagca tcgaaagtag ccagatcagg gatgcgttgc
     5101 aaccgcgtat gcccaggtca gaagagtcgc acaagagttg cagacccctg gaaagaaaaa
     5161 tggccagagg gcgaaaacac cctctgacca gcggagcggg cgacgggaat cgaacccgcg
     5221 tagctagttt ggaagaatgg gtgtctgccg accacatatg ggccggtcaa gataggtttt
     5281 taccccctct cggctgcatc ctctaagtgg aaagaaattg caggtcgtag aagcgcgttg
     5341 aagcctgaga gttgcacagg agttgcaacc cggtagcctt gttcacgacg agaggagacc
     5401 tagttggcac gtcgcggatg gggatcgctg aagactcagc gcagcgggag gatccaagcc
     5461 tcatacgtca acccgcagga cggtgtgagg tactacgcgc tgcagaccta cgacaacaag
     5521 atggacgccg aagcctggct cgcgggcgag aagcggctca tcgagatgga gacctggacc
     5581 cctccacagg accgggcgaa gaaggcagcc gccagcgcca tcacgctgga ggagtacacc
     5641 cggaagtggc tcgtggagcg cgacctcgca gacggcacca gggatctgta cagcgggcac
     5701 gcggagcgcc gcatctaccc ggtgctaggt gaagtggcgg tcacagagat gacgccagct
     5761 ctggtgcgtg cgtggtgggc cgggatgggt aggaagcacc cgactgcccg ccggcatgcc
     5821 tacaacgtcc tccgggcggt gatgaacaca gcggtcgagg acaagctgat cgcagagaac
     5881 ccgtgccgga tcgagcagaa ggcagccgat gagcgcgacg tagaggcgct gacgcctgag
     5941 gagctggaca tcgtcgccgc tgagatcttc gagcactacc ggatcgcggc atacatcctg
     6001 gcgtggacga gcctccggtt cggagagctg atcgagcttc gccgcaagga catcgtggac
     6061 gacggcatga cgatgaagct ccgggtgcgc cgtggcgctt cccgcgtggg gaacaagatc
     6121 gtcgttggca acgccaagac cgtccggtcg aagcgtcctg tgacggttcc gcctcacgtc
     6181 gcggagatga tccgagcgca catgaaggac cgtacgaaga tgaacaaggg ccccgaggca
     6241 ttcctggtga ccacgacgca gggcaaccgg ct
//
