LOCUS       sequence-345476-        6527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0973:Dendra
FEATURES             Location/Qualifiers
     source          1..6527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     665..684
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        665..683
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3090..3113
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3370..4170
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      4295..4883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4784..4803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtcgctgaa gcgtggctac gccaagatcg gtcggccgga actccgcatc cacgacctcc
       61 gcgctgtcgg cgctacgttc gccgctcagg caggtgcgac gaccaaggag ctgatggccc
      121 gtctcggtca cacgactcct aggatggcga tgaagtacca gatggcgtct gaggcccgcg
      181 acgaggctat cgctgaggcg atgtccaagc tggccaagac ctcctgaaac gcaaaaagcc
      241 cccctcccaa ggacactgag tcctaaagag gggggtttct tgtcagtacg cgaagaacca
      301 cgcctggccg cgagcgccag caccgccgct ctgtgcggag acctgggcac cagccccgcc
      361 gccgccagga gcattgccgt tcccgccaga aatctagacg gtgaccacaa cgcgcccgct
      421 ttgatcgggg acgtcgaaat attggatcgt cggcaccgtc acggccgtgg gaggcggcac
      481 gatccgcgac gtgatgatcg gccgcatccc cacggtgctg cgcagtgagc tctacgccat
      541 cccggcgttg atctgtgcgt tcgcacgcac aggcccggtg tgagaagggt ctctgacgag
      601 cgggagaact ccctatcagt gatagagttt gtcctcccta tcagtgatag ataggcttaa
      661 ggagtaatac gactcactat agggagaagg aggccattat gacgtcttcc ccgcgagcag
      721 acacagaact acccgaaaac ccgccggatg cggcaggttt gcgtgtgctc ggcagaagga
      781 tctgggcccc gatccgcaac acgtggcgca cgctgacgtc gatgggcacc gcgctggtgc
      841 tgctgttcct gctcgcgctg ggcgccatcc ccggggccct gctgccgcag cgcagcctca
      901 acgaatccaa ggtcgacgag tacctcgcga cgcacccgac tctcgggccc tggctggacc
      961 gtctgcaggc gttcgacgtg ttctccagct tctggttcac cgcgatctac gtactgctgt
     1021 tcatctcgct ggtgggctgc ctgaccccgc ggatgatcga gcacgtccgc agcctgcgcg
     1081 ccgtgccggt cgccgctccg cgcaacctgg gccgtctgcc caagcaccac gaggcgcagg
     1141 tgcaggccga tgcggccgaa ctcgccgagt ccgtcacgca gcggttgcgg ggctggcgca
     1201 ccgtcacccg cgagaacgtc ggggtcaccg agatctccgc ggagaagggc tatctgcgcg
     1261 agttcggcaa catcgtcttc cacttctcgc tgctgggcct gctcgtcgcg gtcgccgcgg
     1321 gcaagctgtt cggctacgag ggcaacgtga tcgtcatcgc cgacggcggt cccggtttct
     1381 gcacggcctc acccgcggcg ttcgactcgt tccgtgcggg caacaccgtc gacggcacat
     1441 cgctgcaccc gatgtgcatc cgcgtcaaca acttcgatgc ggactacctg ccgaccgggc
     1501 aggcgctgtc attcgccgcc gacatcgact accaggccgg cgacgacctg agcaccgaca
     1561 cgtggcggcc gtaccgcctc gaggtcaacc atccgctgcg cgtcggcggg gaccgcgtgt
     1621 acctgcaggg ccgcggctat gcaccgtcgt tcaccgtcac gttccccgac ggccagaccc
     1681 gcagccagac catccagtgg cgccccgagg aacagatcac gttcctgtcc tccggcgtcg
     1741 tgcgcatcga tccgcccgcg ggcacctacc cggatcccga cgaacgccgc aagcaccaga
     1801 tcgcgatcca gggcctgttc gcgcccacca aacagctcga cggcacgctg ctgtcctcga
     1861 gcttcccggc gctcaacgat ccggccgtcg ccgtggacat ctaccggggc gacaccggcc
     1921 tcgacacggg gcggccacag tcgctgttct cgctggaccc gcggctgatc gggcagaagc
     1981 ggctgaccaa ggtcgcgcgc gtgaacctcg aagagggaca ggagacgcgc ctcgacgacg
     2041 gcaccgtcgt gcggttcgac ggcgcgaagc cgttcgtcaa cctgcaggtg tcccacgatc
     2101 ccgcccaggt gtgggtgctg gtgttcgcga tgacgatgat ggcgggactg ctggtgtcgc
     2161 tcgtggtgcg caggcgccgg gtctgggttc ggatcgaacc cgcaggtgcg ggtaccgtga
     2221 gcgtcgagtt cggcgggctg gcccgcacgg ataactccgg gtggggagac gagttcgagc
     2281 ggttgaccga gcggctgttg gaaggccgca ccgaggcgac cgaatcggcc gaagagaaga
     2341 aggcgaagct tggtgcggcc gcgggcaaca cgcctggcat caatctgatc aaggaggaca
     2401 tgcgtgtcaa ggtgcatatg gagggcaacg tgaacggtca cgccttcgtg atcgaggggg
     2461 agggtaaggg taagccttac gaagggaccc agaccgccaa cctcaccgtc aaggagggcg
     2521 cccctctccc attctcgtac gatatcctca ctaccgcggt gcattacggg aaccgcgtgt
     2581 tcaccaagta ccccgaggat atccctgact acttcaagca gagcttcccg gagggatact
     2641 cctgggagcg gacgatgacc ttcgaagaca agggtatctg tacgatccgc agcgacatct
     2701 cgctggaagg ggactgtttc ttccagaacg tccgtttcaa ggggaccaac tttccgccga
     2761 acggcccggt catgcagaag aagaccctca agtgggagcc gagcacggag aagctgcacg
     2821 ttcgggacgg cctcctggtc ggcaacatca acatggcgct gctgctggaa ggcggcggcc
     2881 actacctatg cgacttcaag accacgtaca aagcgaagaa ggtcgtccag ctgcccgacg
     2941 cgcacttcgt ggaccaccgc atcgaaatcc tggggaacga ttcggactac aacaaggtca
     3001 agttgtacga gcacgctgtg gcacgctata gcccgctccc gtcgcaggtc tggttcgaag
     3061 gcggcggcgg ggagaacctg tatttccagg actacaagga cgacgacgac aagtgattta
     3121 aataacccct tggggcctct aaacgggtct tgaggggttt tttgttaact agcgtacgat
     3181 cgaattcctg cagctcacgg taactgatgc cgtatttgca gtaccagcgt acggcccaca
     3241 gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt catgtgcagc tccatcagca
     3301 aaaggggatg ataagtttat caccaccgac tatttgcaac agtgccgttg atcgtgctat
     3361 gatcgactga tgtcatcagc ggtggagtgc aatgtcgtgc aatacgaatg gcgaaaagcc
     3421 gagctcatcg gtcagcttct caaccttggg gttacccccg gcggtgtgct gctggtccac
     3481 agctccttcc gtagcgtccg gcccctcgaa gatgggccac ttggactgat cgaggccctg
     3541 cgtgctacgc tgggtccggg agggacgctc gtcatgccct cgtggtcagg tctggacgac
     3601 gagccgttcg atcctgccac gtcgcccgtt acaccggacc ttggagttgt ctctgacaca
     3661 ttctggcgcc tgccaaatgt aaagcgcagc gcccatccat ttgcctttgc ggcagcgggg
     3721 ccacaggcag agcagatcat ctctgatcca ttgcccctgc caccttactc gcctgcaagc
     3781 ccggtcgccc gtgtccatga actcgatggg caggtacttc tcctcggcgt gggacacgat
     3841 gccaacacga cgctgcatct tgccgagttg atggcaaagg ttccctatgg ggtgccgaga
     3901 cactgcacca ttcttcagga tggcaagttg gtacgcgtcg attatctcga gaatgaccac
     3961 tgctgtgagc gctttgcctt ggcggacagg tggctcaagg agaagagcct tcagaaggaa
     4021 ggtccagtcg gtcatgcctt tgctcggttg atccgctccc gcgacattgt ggcgacagcc
     4081 ctgggtcaac tgggccgaga tccgttgatc ttcctgcatc cgccagaggc gggatgcgaa
     4141 gaatgcgatg ccgctcgcca gtcgattggc tgagctcatg agcggagaac gagatgacgt
     4201 tggaggggca aggtcgcgct gattgctggg gcaacacggg ggatccacta gttccatgag
     4261 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4321 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4381 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4441 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4501 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4561 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4621 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4681 gtgagctatg agaaagcgcc acgcttcccg aggggagaaa ggcggacagg tatccggtaa
     4741 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4801 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4861 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4921 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     4981 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcaacg
     5041 cgtgcggccg cggtacccgg ggatcctcta gagtcgacca ccaagggcac catctctgct
     5101 tgggccaccc cgttggccgc agccagctcg ctgagagccg tgaacgacag ggcgaacgcc
     5161 agcccgccga cggcgagggt tccgaccgct gcaactcccg gtgcaacctt gtcccggtct
     5221 attctcttca ctgcaccagc tccaatctgg tgtgaatgcc cctcgtctgt tcgcgcaggc
     5281 ggggggctct attcgtttgt cagcatcgaa agtagccaga tcagggatgc gttgcaaccg
     5341 cgtatgccca ggtcagaaga gtcgcacaag agttgcagac ccctggaaag aaaaatggcc
     5401 agagggcgaa aacaccctct gaccagcgga gcgggcgacg ggaatcgaac ccgcgtagct
     5461 agtttggaag aatgggtgtc tgccgaccac atatgggccg gtcaagatag gtttttaccc
     5521 cctctcggct gcatcctcta agtggaaaga aattgcaggt cgtagaagcg cgttgaagcc
     5581 tgagagttgc acaggagttg caacccggta gccttgttca cgacgagagg agacctagtt
     5641 ggcacgtcgc ggatggggat cgctgaagac tcagcgcagc gggaggatcc aagcctcata
     5701 cgtcaacccg caggacggtg tgaggtacta cgcgctgcag acctacgaca acaagatgga
     5761 cgccgaagcc tggctcgcgg gcgagaagcg gctcatcgag atggagacct ggacccctcc
     5821 acaggaccgg gcgaagaagg cagccgccag cgccatcacg ctggaggagt acacccggaa
     5881 gtggctcgtg gagcgcgacc tcgcagacgg caccagggat ctgtacagcg ggcacgcgga
     5941 gcgccgcatc tacccggtgc taggtgaagt ggcggtcaca gagatgacgc cagctctggt
     6001 gcgtgcgtgg tgggccggga tgggtaggaa gcacccgact gcccgccggc atgcctacaa
     6061 cgtcctccgg gcggtgatga acacagcggt cgaggacaag ctgatcgcag agaacccgtg
     6121 ccggatcgag cagaaggcag ccgatgagcg cgacgtagag gcgctgacgc ctgaggagct
     6181 ggacatcgtc gccgctgaga tcttcgagca ctaccggatc gcggcataca tcctggcgtg
     6241 gacgagcctc cggttcggag agctgatcga gcttcgccgc aaggacatcg tggacgacgg
     6301 catgacgatg aagctccggg tgcgccgtgg cgcttcccgc gtggggaaca agatcgtcgt
     6361 tggcaacgcc aagaccgtcc ggtcgaagcg tcctgtgacg gttccgcctc acgtcgcgga
     6421 gatgatccga gcgcacatga aggaccgtac gaagatgaac aagggccccg aggcattcct
     6481 ggtgaccacg acgcagggca accggctgtc gaagtccgcg ttcacca
//
