LOCUS       sequence-345478-        5918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1062:Dendra
FEATURES             Location/Qualifiers
     source          1..5918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     746..765
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        746..764
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2562..2585
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2842..3642
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3767..4355
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4256..4275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtacgaagat gaacaagggc cccgaggcat tcctggtgac cacgacgcag ggcaaccggc
       61 tgtcgaagtc cgcgttcacc aagtcgctga agcgtggcta cgccaagatc ggtcggccgg
      121 aactccgcat ccacgacctc cgcgctgtcg gcgctacgtt cgccgctcag gcaggtgcga
      181 cgaccaagga gctgatggcc cgtctcggtc acacgactcc taggatggcg atgaagtacc
      241 agatggcgtc tgaggcccgc gacgaggcta tcgctgaggc gatgtccaag ctggccaaga
      301 cctcctgaaa cgcaaaaagc ccccctccca aggacactga gtcctaaaga ggggggtttc
      361 ttgtcagtac gcgaagaacc acgcctggcc gcgagcgcca gcaccgccgc tctgtgcgga
      421 gacctgggca ccagccccgc cgccgccagg agcattgccg ttcccgccag aaatctagac
      481 ggtgaccaca acgcgcccgc tttgatcggg gacgtcgaaa tattggatcg tcggcaccgt
      541 cacggccgtg ggaggcggca cgatccgcga cgtgatgatc ggccgcatcc ccacggtgct
      601 gcgcagtgag ctctacgcca tcccggcgtt gatctgtgcg ttcgcacgca caggcccggt
      661 gtgagaaggg tctctgacga gcgggagaac tccctatcag tgatagagtt tgtcctccct
      721 atcagtgata gataggctta aggagtaata cgactcacta tagggagaag gaggccatta
      781 tgcctgctgc ggaccgacgc cagaccgcgc tgctgacatc gacgttgcgc gccggagaat
      841 ccatcgacga cgacgtcgcc gtggtggtct cgacgtcggg gacgacgggt gcacccaagg
      901 gtgcgttgct gacacgcagc gccctggtct ccagtgccga ggccacctac gaacgcctcg
      961 gcggggcggg ccgatggttg ctggcactgc ccgcccacca catcgccggg ttgcaggtgc
     1021 tggtgcgcag caccgtggcg ggcaccacac ccgtgaccat tcccgccggt ttcgaacccg
     1081 gcgaattgcc ctctgccata gcgcgattgg gcccgggacg caaatacgcg tcgatggtcg
     1141 cgatccaact cgacaaggca ctgcgcgatc cggcggcaac cacggcgctg gccgaactcg
     1201 acgcggtgct catcggcggc ggacccatgc ctgcgggtat ggccgaacgc gctgccgcgg
     1261 gcggggtccg agtggtccgt acctacggca tgagcgagac cgcgggcgga tgcgtgtacg
     1321 acggggtgcc gctcgcgggg gtgacgatcc gcatcgagga ctcgcggatc tcactcggcg
     1381 gcgcgacggt cgcgaagggc taccgcaatc cggtggagcc ggacccgttt tccgaaccgg
     1441 gctggtttcg caccgatgac cttggcgtcc ttgacgattc gggtgcgctg cgcgtgctgg
     1501 gacgcatcga cgacgcggtg agcacaggcg gcctgaccgt gctgccccaa ctcgtcgagg
     1561 ccgcgttggt gacccacccc gcgatcgccg aggccgcggt gttcggggtg gccgacgaac
     1621 ggttgggaca gcgcgtggtg gccgcggtgg tgctcgcggc gggttcaccg ctccccgaac
     1681 tcgccgaact gcgctcgcac gtcgcgctga ccctcgacgc cacggccgcg ccacgcgagg
     1741 tgcatgtggt cgacgaactg ccccggcacg gcatcggaaa gctcgaccgg cgcgccctgg
     1801 tggcccggtt cggcggtaag cttggtgcgg ccgcgggcaa cacgcctggc atcaatctga
     1861 tcaaggagga catgcgtgtc aaggtgcata tggagggcaa cgtgaacggt cacgccttcg
     1921 tgatcgaggg ggagggtaag ggtaagcctt acgaagggac ccagaccgcc aacctcaccg
     1981 tcaaggaggg cgcccctctc ccattctcgt acgatatcct cactaccgcg gtgcattacg
     2041 ggaaccgcgt gttcaccaag taccccgagg atatccctga ctacttcaag cagagcttcc
     2101 cggagggata ctcctgggag cggacgatga ccttcgaaga caagggtatc tgtacgatcc
     2161 gcagcgacat ctcgctggaa ggggactgtt tcttccagaa cgtccgtttc aaggggacca
     2221 actttccgcc gaacggcccg gtcatgcaga agaagaccct caagtgggag ccgagcacgg
     2281 agaagctgca cgttcgggac ggcctcctgg tcggcaacat caacatggcg ctgctgctgg
     2341 aaggcggcgg ccactaccta tgcgacttca agaccacgta caaagcgaag aaggtcgtcc
     2401 agctgcccga cgcgcacttc gtggaccacc gcatcgaaat cctggggaac gattcggact
     2461 acaacaaggt caagttgtac gagcacgctg tggcacgcta tagcccgctc ccgtcgcagg
     2521 tctggttcga aggcggcggc ggggagaacc tgtatttcca ggactacaag gacgacgacg
     2581 acaagtgatt taaataaccc cttggggcct ctaaacgggt cttgaggggt tttttgttaa
     2641 ctagcgtacg atcgaattcc tgcagctcac ggtaactgat gccgtatttg cagtaccagc
     2701 gtacggccca cagaatgatg tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca
     2761 gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt
     2821 tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa
     2881 tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg
     2941 ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg
     3001 atcgaggccc tgcgtgctac gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca
     3061 ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt
     3121 gtctctgaca cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt
     3181 gcggcagcgg ggccacaggc agagcagatc atctctgatc cattgcccct gccaccttac
     3241 tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc
     3301 gtgggacacg atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat
     3361 ggggtgccga gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc
     3421 gagaatgacc actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc
     3481 cttcagaagg aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt
     3541 gtggcgacag ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag
     3601 gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga
     3661 acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg ggggatccac
     3721 tagttccatg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3781 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3841 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3901 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3961 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4021 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4081 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4141 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaggggaga aaggcggaca
     4201 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4261 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4321 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4381 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4441 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4501 ccgagcgcaa cgcgtgcggc cgcggtaccc ggggatcctc tagagtcgac caccaagggc
     4561 accatctctg cttgggccac cccgttggcc gcagccagct cgctgagagc cgtgaacgac
     4621 agggcgaacg ccagcccgcc gacggcgagg gttccgaccg ctgcaactcc cggtgcaacc
     4681 ttgtcccggt ctattctctt cactgcacca gctccaatct ggtgtgaatg cccctcgtct
     4741 gttcgcgcag gcggggggct ctattcgttt gtcagcatcg aaagtagcca gatcagggat
     4801 gcgttgcaac cgcgtatgcc caggtcagaa gagtcgcaca agagttgcag acccctggaa
     4861 agaaaaatgg ccagagggcg aaaacaccct ctgaccagcg gagcgggcga cgggaatcga
     4921 acccgcgtag ctagtttgga agaatgggtg tctgccgacc acatatgggc cggtcaagat
     4981 aggtttttac cccctctcgg ctgcatcctc taagtggaaa gaaattgcag gtcgtagaag
     5041 cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg tagccttgtt cacgacgaga
     5101 ggagacctag ttggcacgtc gcggatgggg atcgctgaag actcagcgca gcgggaggat
     5161 ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac tacgcgctgc agacctacga
     5221 caacaagatg gacgccgaag cctggctcgc gggcgagaag cggctcatcg agatggagac
     5281 ctggacccct ccacaggacc gggcgaagaa ggcagccgcc agcgccatca cgctggagga
     5341 gtacacccgg aagtggctcg tggagcgcga cctcgcagac ggcaccaggg atctgtacag
     5401 cgggcacgcg gagcgccgca tctacccggt gctaggtgaa gtggcggtca cagagatgac
     5461 gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg aagcacccga ctgcccgccg
     5521 gcatgcctac aacgtcctcc gggcggtgat gaacacagcg gtcgaggaca agctgatcgc
     5581 agagaacccg tgccggatcg agcagaaggc agccgatgag cgcgacgtag aggcgctgac
     5641 gcctgaggag ctggacatcg tcgccgctga gatcttcgag cactaccgga tcgcggcata
     5701 catcctggcg tggacgagcc tccggttcgg agagctgatc gagcttcgcc gcaaggacat
     5761 cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt ggcgcttccc gcgtggggaa
     5821 caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag cgtcctgtga cggttccgcc
     5881 tcacgtcgcg gagatgatcc gagcgcacat gaaggacc
//
