LOCUS       sequence-345479-        5567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1115:Dendra
FEATURES             Location/Qualifiers
     source          1..5567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     646..665
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        646..664
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2111..2134
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2391..3191
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3316..3904
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3805..3824
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgccaagatc ggtcggccgg aactccgcat ccacgacctc cgcgctgtcg gcgctacgtt
       61 cgccgctcag gcaggtgcga cgaccaagga gctgatggcc cgtctcggtc acacgactcc
      121 taggatggcg atgaagtacc agatggcgtc tgaggcccgc gacgaggcta tcgctgaggc
      181 gatgtccaag ctggccaaga cctcctgaaa cgcaaaaagc ccccctccca aggacactga
      241 gtcctaaaga ggggggtttc ttgtcagtac gcgaagaacc acgcctggcc gcgagcgcca
      301 gcaccgccgc tctgtgcgga gacctgggca ccagccccgc cgccgccagg agcattgccg
      361 ttcccgccag aaatctagac ggtgaccaca acgcgcccgc tttgatcggg gacgtcgaaa
      421 tattggatcg tcggcaccgt cacggccgtg ggaggcggca cgatccgcga cgtgatgatc
      481 ggccgcatcc ccacggtgct gcgcagtgag ctctacgcca tcccggcgtt gatctgtgcg
      541 ttcgcacgca caggcccggt gtgagaaggg tctctgacga gcgggagaac tccctatcag
      601 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
      661 tagggagaag gaggccatta tgagtcgagc gagcttggag aagaatcccc acgacgtggc
      721 atcgatgttc gacgccgtcg cgaagcgcta cgacctgacc aacacggtgc tgtcgctcgg
      781 tcaggaccgg ttctggcgtc gcgagaccaa gtcggccctg cgtatcgggc cagcggacaa
      841 ggtgctggac ctggccgccg ggaccgcggt gtccaccgtc gagctcgcca agtccggcgc
      901 gtggtgtgtg gccgcggact tctcggtcgg catgctggcc gccggggcgt ctcgccaggt
      961 acccaaggtt gccggcgacg ccacgcggct gccgttcgcc gacgaggtct tcgacgccgt
     1021 caccatcagc ttcggtctgc gcaacgtcgt cgaccacaag gcaggcctgc gcgagatggc
     1081 gcgggtgacc aagccgggcg ggcgcctggt ggtgtgcgag ttctccacgc ccaccaaccg
     1141 cctgttctcc acggtctaca aggagtattt gatgaaggcg ctgcctgcca tggccacggc
     1201 ggtgtcgagc aatcccgagg cctacgtcta cctcgccgag tccatccgcg cctggcccac
     1261 ccaggccgaa ctcgcccgtc agatcggcga ggccggctgg tccgacgtcc gctggcgcaa
     1321 cctcaccggc ggcatcgtgg ccctgcacgc cgccaccaag cccgccaagc ttggtgcggc
     1381 cgcgggcaac acgcctggca tcaatctgat caaggaggac atgcgtgtca aggtgcatat
     1441 ggagggcaac gtgaacggtc acgccttcgt gatcgagggg gagggtaagg gtaagcctta
     1501 cgaagggacc cagaccgcca acctcaccgt caaggagggc gcccctctcc cattctcgta
     1561 cgatatcctc actaccgcgg tgcattacgg gaaccgcgtg ttcaccaagt accccgagga
     1621 tatccctgac tacttcaagc agagcttccc ggagggatac tcctgggagc ggacgatgac
     1681 cttcgaagac aagggtatct gtacgatccg cagcgacatc tcgctggaag gggactgttt
     1741 cttccagaac gtccgtttca aggggaccaa ctttccgccg aacggcccgg tcatgcagaa
     1801 gaagaccctc aagtgggagc cgagcacgga gaagctgcac gttcgggacg gcctcctggt
     1861 cggcaacatc aacatggcgc tgctgctgga aggcggcggc cactacctat gcgacttcaa
     1921 gaccacgtac aaagcgaaga aggtcgtcca gctgcccgac gcgcacttcg tggaccaccg
     1981 catcgaaatc ctggggaacg attcggacta caacaaggtc aagttgtacg agcacgctgt
     2041 ggcacgctat agcccgctcc cgtcgcaggt ctggttcgaa ggcggcggcg gggagaacct
     2101 gtatttccag gactacaagg acgacgacga caagtgattt aaataacccc ttggggcctc
     2161 taaacgggtc ttgaggggtt ttttgttaac tagcgtacga tcgaattcct gcagctcacg
     2221 gtaactgatg ccgtatttgc agtaccagcg tacggcccac agaatgatgt cacgctgaaa
     2281 atgccggcct ttgaatgggt tcatgtgcag ctccatcagc aaaaggggat gataagttta
     2341 tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta tgatcgactg atgtcatcag
     2401 cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc cgagctcatc ggtcagcttc
     2461 tcaaccttgg ggttaccccc ggcggtgtgc tgctggtcca cagctccttc cgtagcgtcc
     2521 ggcccctcga agatgggcca cttggactga tcgaggccct gcgtgctacg ctgggtccgg
     2581 gagggacgct cgtcatgccc tcgtggtcag gtctggacga cgagccgttc gatcctgcca
     2641 cgtcgcccgt tacaccggac cttggagttg tctctgacac attctggcgc ctgccaaatg
     2701 taaagcgcag cgcccatcca tttgcctttg cggcagcggg gccacaggca gagcagatca
     2761 tctctgatcc attgcccctg ccaccttact cgcctgcaag cccggtcgcc cgtgtccatg
     2821 aactcgatgg gcaggtactt ctcctcggcg tgggacacga tgccaacacg acgctgcatc
     2881 ttgccgagtt gatggcaaag gttccctatg gggtgccgag acactgcacc attcttcagg
     2941 atggcaagtt ggtacgcgtc gattatctcg agaatgacca ctgctgtgag cgctttgcct
     3001 tggcggacag gtggctcaag gagaagagcc ttcagaagga aggtccagtc ggtcatgcct
     3061 ttgctcggtt gatccgctcc cgcgacattg tggcgacagc cctgggtcaa ctgggccgag
     3121 atccgttgat cttcctgcat ccgccagagg cgggatgcga agaatgcgat gccgctcgcc
     3181 agtcgattgg ctgagctcat gagcggagaa cgagatgacg ttggaggggc aaggtcgcgc
     3241 tgattgctgg ggcaacacgg gggatccact agttccatga gcgtcagacc ccgtagaaaa
     3301 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3361 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3421 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     3481 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3541 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3601 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3661 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3721 cacgcttccc gaggggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3781 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3841 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3901 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3961 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4021 agctgatacc gctcgccgca gccgaacgac cgagcgcaac gcgtgcggcc gcggtacccg
     4081 gggatcctct agagtcgacc accaagggca ccatctctgc ttgggccacc ccgttggccg
     4141 cagccagctc gctgagagcc gtgaacgaca gggcgaacgc cagcccgccg acggcgaggg
     4201 ttccgaccgc tgcaactccc ggtgcaacct tgtcccggtc tattctcttc actgcaccag
     4261 ctccaatctg gtgtgaatgc ccctcgtctg ttcgcgcagg cggggggctc tattcgtttg
     4321 tcagcatcga aagtagccag atcagggatg cgttgcaacc gcgtatgccc aggtcagaag
     4381 agtcgcacaa gagttgcaga cccctggaaa gaaaaatggc cagagggcga aaacaccctc
     4441 tgaccagcgg agcgggcgac gggaatcgaa cccgcgtagc tagtttggaa gaatgggtgt
     4501 ctgccgacca catatgggcc ggtcaagata ggtttttacc ccctctcggc tgcatcctct
     4561 aagtggaaag aaattgcagg tcgtagaagc gcgttgaagc ctgagagttg cacaggagtt
     4621 gcaacccggt agccttgttc acgacgagag gagacctagt tggcacgtcg cggatgggga
     4681 tcgctgaaga ctcagcgcag cgggaggatc caagcctcat acgtcaaccc gcaggacggt
     4741 gtgaggtact acgcgctgca gacctacgac aacaagatgg acgccgaagc ctggctcgcg
     4801 ggcgagaagc ggctcatcga gatggagacc tggacccctc cacaggaccg ggcgaagaag
     4861 gcagccgcca gcgccatcac gctggaggag tacacccgga agtggctcgt ggagcgcgac
     4921 ctcgcagacg gcaccaggga tctgtacagc gggcacgcgg agcgccgcat ctacccggtg
     4981 ctaggtgaag tggcggtcac agagatgacg ccagctctgg tgcgtgcgtg gtgggccggg
     5041 atgggtagga agcacccgac tgcccgccgg catgcctaca acgtcctccg ggcggtgatg
     5101 aacacagcgg tcgaggacaa gctgatcgca gagaacccgt gccggatcga gcagaaggca
     5161 gccgatgagc gcgacgtaga ggcgctgacg cctgaggagc tggacatcgt cgccgctgag
     5221 atcttcgagc actaccggat cgcggcatac atcctggcgt ggacgagcct ccggttcgga
     5281 gagctgatcg agcttcgccg caaggacatc gtggacgacg gcatgacgat gaagctccgg
     5341 gtgcgccgtg gcgcttcccg cgtggggaac aagatcgtcg ttggcaacgc caagaccgtc
     5401 cggtcgaagc gtcctgtgac ggttccgcct cacgtcgcgg agatgatccg agcgcacatg
     5461 aaggaccgta cgaagatgaa caagggcccc gaggcattcc tggtgaccac gacgcagggc
     5521 aaccggctgt cgaagtccgc gttcaccaag tcgctgaagc gtggcta
//
