LOCUS       sequence-345480-        5873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0974:Dendra
FEATURES             Location/Qualifiers
     source          1..5873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     687..706
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        687..705
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2458..2481
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2738..3538
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3663..4251
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4152..4171
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgtcgaagt ccgcgttcac caagtcgctg aagcgtggct acgccaagat cggtcggccg
       61 gaactccgca tccacgacct ccgcgctgtc ggcgctacgt tcgccgctca ggcaggtgcg
      121 acgaccaagg agctgatggc ccgtctcggt cacacgactc ctaggatggc gatgaagtac
      181 cagatggcgt ctgaggcccg cgacgaggct atcgctgagg cgatgtccaa gctggccaag
      241 acctcctgaa acgcaaaaag cccccctccc aaggacactg agtcctaaag aggggggttt
      301 cttgtcagta cgcgaagaac cacgcctggc cgcgagcgcc agcaccgccg ctctgtgcgg
      361 agacctgggc accagccccg ccgccgccag gagcattgcc gttcccgcca gaaatctaga
      421 cggtgaccac aacgcgcccg ctttgatcgg ggacgtcgaa atattggatc gtcggcaccg
      481 tcacggccgt gggaggcggc acgatccgcg acgtgatgat cggccgcatc cccacggtgc
      541 tgcgcagtga gctctacgcc atcccggcgt tgatctgtgc gttcgcacgc acaggcccgg
      601 tgtgagaagg gtctctgacg agcgggagaa ctccctatca gtgatagagt ttgtcctccc
      661 tatcagtgat agataggctt aaggagtaat acgactcact atagggagaa ggaggccatt
      721 atgatgaata ccgagcacat cgatatcggg ctggcccggt actccgactg ggcgttcacg
      781 tcctcggtgc tggtgctggt gggtgcactg ctgctgctcg ccgtcgagat ggcgtacagc
      841 cgcagccgcc gcgtcgagtc ccgtgagctg gtcggggcgg gcgggccctc ggtggtcggc
      901 gccgacagcg cgacccccgg cgtcgtcgtg gagagcccca agcggccgtt cgacgagcgg
      961 gtcggcaaga ccggtctggc actgacctac gtcggtatcg gtctgctgct ggtgtgcatc
     1021 gtgctgcgcg gcgtgtccac ctcgcgcgtg ccgtggggca acatgtacga gttcatcaac
     1081 ctgacgtgct tctgcgggct ggtggccgcc gcggtcgtgc tgcgcaggcc gcagtaccgc
     1141 acgctgtggg tgttcgtgct cgtgccggtg ctgatcctgt tgaccgtctc gggccgctgg
     1201 ctctacacca acgccgcccc ggtgatgccc gcgctgcagt cgtactggct gcccatccac
     1261 gtgtcggtgg tgagcctggg ctcgggtgtg ttcctggtgg cgggcgtcgc gagcatcctg
     1321 ttcctgctga agatgtcgcc gctgggccaa ccgcaggagg ggcgtgacag cgtgtgggcg
     1381 cggatcatcg accgcgtgcc cgacgcgcag acgctggacc ggatcgccta ccgcaccacg
     1441 atcttcgcgt tcccggtgtt cggcttcggc gtcatcttcg gcgcgatctg ggccgaggag
     1501 gcctggggcc gctactgggg ctgggacccc aaggagaccg tgtcgttcat cgcgtgggtc
     1561 gtgtacgcgg cgtacctgca cgcccgctcg accgcggggt ggcgtgaccg caaggccgcc
     1621 tggatcaacg tcgtcggttt cgtcgcgatg gtcttcaacc tgttcttcat caacctggtc
     1681 accgtcggcc tgcattcgta cgcgggagtc ggcaagcttg gtgcggccgc gggcaacacg
     1741 cctggcatca atctgatcaa ggaggacatg cgtgtcaagg tgcatatgga gggcaacgtg
     1801 aacggtcacg ccttcgtgat cgagggggag ggtaagggta agccttacga agggacccag
     1861 accgccaacc tcaccgtcaa ggagggcgcc cctctcccat tctcgtacga tatcctcact
     1921 accgcggtgc attacgggaa ccgcgtgttc accaagtacc ccgaggatat ccctgactac
     1981 ttcaagcaga gcttcccgga gggatactcc tgggagcgga cgatgacctt cgaagacaag
     2041 ggtatctgta cgatccgcag cgacatctcg ctggaagggg actgtttctt ccagaacgtc
     2101 cgtttcaagg ggaccaactt tccgccgaac ggcccggtca tgcagaagaa gaccctcaag
     2161 tgggagccga gcacggagaa gctgcacgtt cgggacggcc tcctggtcgg caacatcaac
     2221 atggcgctgc tgctggaagg cggcggccac tacctatgcg acttcaagac cacgtacaaa
     2281 gcgaagaagg tcgtccagct gcccgacgcg cacttcgtgg accaccgcat cgaaatcctg
     2341 gggaacgatt cggactacaa caaggtcaag ttgtacgagc acgctgtggc acgctatagc
     2401 ccgctcccgt cgcaggtctg gttcgaaggc ggcggcgggg agaacctgta tttccaggac
     2461 tacaaggacg acgacgacaa gtgatttaaa taaccccttg gggcctctaa acgggtcttg
     2521 aggggttttt tgttaactag cgtacgatcg aattcctgca gctcacggta actgatgccg
     2581 tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg ccggcctttg
     2641 aatgggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca ccaccgacta
     2701 tttgcaacag tgccgttgat cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa
     2761 tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt cagcttctca accttggggt
     2821 tacccccggc ggtgtgctgc tggtccacag ctccttccgt agcgtccggc ccctcgaaga
     2881 tgggccactt ggactgatcg aggccctgcg tgctacgctg ggtccgggag ggacgctcgt
     2941 catgccctcg tggtcaggtc tggacgacga gccgttcgat cctgccacgt cgcccgttac
     3001 accggacctt ggagttgtct ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc
     3061 ccatccattt gcctttgcgg cagcggggcc acaggcagag cagatcatct ctgatccatt
     3121 gcccctgcca ccttactcgc ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca
     3181 ggtacttctc ctcggcgtgg gacacgatgc caacacgacg ctgcatcttg ccgagttgat
     3241 ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt cttcaggatg gcaagttggt
     3301 acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc tttgccttgg cggacaggtg
     3361 gctcaaggag aagagccttc agaaggaagg tccagtcggt catgcctttg ctcggttgat
     3421 ccgctcccgc gacattgtgg cgacagccct gggtcaactg ggccgagatc cgttgatctt
     3481 cctgcatccg ccagaggcgg gatgcgaaga atgcgatgcc gctcgccagt cgattggctg
     3541 agctcatgag cggagaacga gatgacgttg gaggggcaag gtcgcgctga ttgctggggc
     3601 aacacggggg atccactagt tccatgagcg tcagaccccg tagaaaagat caaaggatct
     3661 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3721 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3781 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     3841 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3901 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3961 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4021 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgag
     4081 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4141 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4201 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4261 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     4321 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     4381 cgccgcagcc gaacgaccga gcgcaacgcg tgcggccgcg gtacccgggg atcctctaga
     4441 gtcgaccacc aagggcacca tctctgcttg ggccaccccg ttggccgcag ccagctcgct
     4501 gagagccgtg aacgacaggg cgaacgccag cccgccgacg gcgagggttc cgaccgctgc
     4561 aactcccggt gcaaccttgt cccggtctat tctcttcact gcaccagctc caatctggtg
     4621 tgaatgcccc tcgtctgttc gcgcaggcgg ggggctctat tcgtttgtca gcatcgaaag
     4681 tagccagatc agggatgcgt tgcaaccgcg tatgcccagg tcagaagagt cgcacaagag
     4741 ttgcagaccc ctggaaagaa aaatggccag agggcgaaaa caccctctga ccagcggagc
     4801 gggcgacggg aatcgaaccc gcgtagctag tttggaagaa tgggtgtctg ccgaccacat
     4861 atgggccggt caagataggt ttttaccccc tctcggctgc atcctctaag tggaaagaaa
     4921 ttgcaggtcg tagaagcgcg ttgaagcctg agagttgcac aggagttgca acccggtagc
     4981 cttgttcacg acgagaggag acctagttgg cacgtcgcgg atggggatcg ctgaagactc
     5041 agcgcagcgg gaggatccaa gcctcatacg tcaacccgca ggacggtgtg aggtactacg
     5101 cgctgcagac ctacgacaac aagatggacg ccgaagcctg gctcgcgggc gagaagcggc
     5161 tcatcgagat ggagacctgg acccctccac aggaccgggc gaagaaggca gccgccagcg
     5221 ccatcacgct ggaggagtac acccggaagt ggctcgtgga gcgcgacctc gcagacggca
     5281 ccagggatct gtacagcggg cacgcggagc gccgcatcta cccggtgcta ggtgaagtgg
     5341 cggtcacaga gatgacgcca gctctggtgc gtgcgtggtg ggccgggatg ggtaggaagc
     5401 acccgactgc ccgccggcat gcctacaacg tcctccgggc ggtgatgaac acagcggtcg
     5461 aggacaagct gatcgcagag aacccgtgcc ggatcgagca gaaggcagcc gatgagcgcg
     5521 acgtagaggc gctgacgcct gaggagctgg acatcgtcgc cgctgagatc ttcgagcact
     5581 accggatcgc ggcatacatc ctggcgtgga cgagcctccg gttcggagag ctgatcgagc
     5641 ttcgccgcaa ggacatcgtg gacgacggca tgacgatgaa gctccgggtg cgccgtggcg
     5701 cttcccgcgt ggggaacaag atcgtcgttg gcaacgccaa gaccgtccgg tcgaagcgtc
     5761 ctgtgacggt tccgcctcac gtcgcggaga tgatccgagc gcacatgaag gaccgtacga
     5821 agatgaacaa gggccccgag gcattcctgg tgaccacgac gcagggcaac cgg
//
