LOCUS       sequence-345481-        5527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0972:Dendra
FEATURES             Location/Qualifiers
     source          1..5527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1217..1236
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1217..1235
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2778..2801
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3058..3858
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3983..4571
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4472..4491
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcacagagat gacgccagct ctggtgcgtg cgtggtgggc cgggatgggt aggaagcacc
       61 cgactgcccg ccggcatgcc tacaacgtcc tccgggcggt gatgaacaca gcggtcgagg
      121 acaagctgat cgcagagaac ccgtgccgga tcgagcagaa ggcagccgat gagcgcgacg
      181 tagaggcgct gacgcctgag gagctggaca tcgtcgccgc tgagatcttc gagcactacc
      241 ggatcgcggc atacatcctg gcgtggacga gcctccggtt cggagagctg atcgagcttc
      301 gccgcaagga catcgtggac gacggcatga cgatgaagct ccgggtgcgc cgtggcgctt
      361 cccgcgtggg gaacaagatc gtcgttggca acgccaagac cgtccggtcg aagcgtcctg
      421 tgacggttcc gcctcacgtc gcggagatga tccgagcgca catgaaggac cgtacgaaga
      481 tgaacaaggg ccccgaggca ttcctggtga ccacgacgca gggcaaccgg ctgtcgaagt
      541 ccgcgttcac caagtcgctg aagcgtggct acgccaagat cggtcggccg gaactccgca
      601 tccacgacct ccgcgctgtc ggcgctacgt tcgccgctca ggcaggtgcg acgaccaagg
      661 agctgatggc ccgtctcggt cacacgactc ctaggatggc gatgaagtac cagatggcgt
      721 ctgaggcccg cgacgaggct atcgctgagg cgatgtccaa gctggccaag acctcctgaa
      781 acgcaaaaag cccccctccc aaggacactg agtcctaaag aggggggttt cttgtcagta
      841 cgcgaagaac cacgcctggc cgcgagcgcc agcaccgccg ctctgtgcgg agacctgggc
      901 accagccccg ccgccgccag gagcattgcc gttcccgcca gaaatctaga cggtgaccac
      961 aacgcgcccg ctttgatcgg ggacgtcgaa atattggatc gtcggcaccg tcacggccgt
     1021 gggaggcggc acgatccgcg acgtgatgat cggccgcatc cccacggtgc tgcgcagtga
     1081 gctctacgcc atcccggcgt tgatctgtgc gttcgcacgc acaggcccgg tgtgagaagg
     1141 gtctctgacg agcgggagaa ctccctatca gtgatagagt ttgtcctccc tatcagtgat
     1201 agataggctt aaggagtaat acgactcact atagggagaa ggaggccatt atgaccggat
     1261 tcgccgagat cgcggcggca ggaccggttc tgctcgccct gggcgtgagt gtgctggccg
     1321 gcctggtgtc gttcgcctcg ccgtgcgtcg tgccgctcgt gcccggctac ctgtcgtatc
     1381 tggccgcggt ggtcggtgtc gacgacgatc ccgtgcgcac cggcggtgag cgcgtcgcgc
     1441 tgcgctcggc acggctgcgg gtcgcgggtg cggcggcact gttcgtcgcc gggttcaccg
     1501 tggtgttcct gctgggtgcg gtcgcggtgc tcggcatgac caccaccttg atcgccaatc
     1561 agcttctgct gcaacgcatc ggcggtgtcg tcacgatcgt catgggcctg gtgttcgtgg
     1621 gcttcatccc ggcgctgcag cggcaggcgc ggttcacccc gcgccaattg tcgacggtcg
     1681 cgggcgcacc gctgctgggt gcggtgttcg cgctcggttg gacgccgtgc ctggggccga
     1741 cgctcaccgg cgtcatcgcg gtcgcctcgg ccaccgacgg cgccacggtc gcgcgcggtg
     1801 tcgtgctggt gctggcctac tgcctggggc tcggcatccc gttcgtactg ctcgcgctgg
     1861 gctcggcgcg cgccgtgcag ggtttgggct ggctgcgcag gcacaccagg accatccaga
     1921 tcttcggcgg cgtgctgctg atcctcgtcg gcgcggccct cgtcacgggc ctgtggaacg
     1981 agttcgtgtc ctacgtgcgc gacgccttcg tcagtgatgt gaggctcccc atcaagcttg
     2041 gtgcggccgc gggcaacacg cctggcatca atctgatcaa ggaggacatg cgtgtcaagg
     2101 tgcatatgga gggcaacgtg aacggtcacg ccttcgtgat cgagggggag ggtaagggta
     2161 agccttacga agggacccag accgccaacc tcaccgtcaa ggagggcgcc cctctcccat
     2221 tctcgtacga tatcctcact accgcggtgc attacgggaa ccgcgtgttc accaagtacc
     2281 ccgaggatat ccctgactac ttcaagcaga gcttcccgga gggatactcc tgggagcgga
     2341 cgatgacctt cgaagacaag ggtatctgta cgatccgcag cgacatctcg ctggaagggg
     2401 actgtttctt ccagaacgtc cgtttcaagg ggaccaactt tccgccgaac ggcccggtca
     2461 tgcagaagaa gaccctcaag tgggagccga gcacggagaa gctgcacgtt cgggacggcc
     2521 tcctggtcgg caacatcaac atggcgctgc tgctggaagg cggcggccac tacctatgcg
     2581 acttcaagac cacgtacaaa gcgaagaagg tcgtccagct gcccgacgcg cacttcgtgg
     2641 accaccgcat cgaaatcctg gggaacgatt cggactacaa caaggtcaag ttgtacgagc
     2701 acgctgtggc acgctatagc ccgctcccgt cgcaggtctg gttcgaaggc ggcggcgggg
     2761 agaacctgta tttccaggac tacaaggacg acgacgacaa gtgatttaaa taaccccttg
     2821 gggcctctaa acgggtcttg aggggttttt tgttaactag cgtacgatcg aattcctgca
     2881 gctcacggta actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac
     2941 gctgaaaatg ccggcctttg aatgggttca tgtgcagctc catcagcaaa aggggatgat
     3001 aagtttatca ccaccgacta tttgcaacag tgccgttgat cgtgctatga tcgactgatg
     3061 tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt
     3121 cagcttctca accttggggt tacccccggc ggtgtgctgc tggtccacag ctccttccgt
     3181 agcgtccggc ccctcgaaga tgggccactt ggactgatcg aggccctgcg tgctacgctg
     3241 ggtccgggag ggacgctcgt catgccctcg tggtcaggtc tggacgacga gccgttcgat
     3301 cctgccacgt cgcccgttac accggacctt ggagttgtct ctgacacatt ctggcgcctg
     3361 ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg cagcggggcc acaggcagag
     3421 cagatcatct ctgatccatt gcccctgcca ccttactcgc ctgcaagccc ggtcgcccgt
     3481 gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg gacacgatgc caacacgacg
     3541 ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt
     3601 cttcaggatg gcaagttggt acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc
     3661 tttgccttgg cggacaggtg gctcaaggag aagagccttc agaaggaagg tccagtcggt
     3721 catgcctttg ctcggttgat ccgctcccgc gacattgtgg cgacagccct gggtcaactg
     3781 ggccgagatc cgttgatctt cctgcatccg ccagaggcgg gatgcgaaga atgcgatgcc
     3841 gctcgccagt cgattggctg agctcatgag cggagaacga gatgacgttg gaggggcaag
     3901 gtcgcgctga ttgctggggc aacacggggg atccactagt tccatgagcg tcagaccccg
     3961 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4021 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4081 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     4141 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4201 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4261 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4321 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4381 aaagcgccac gcttcccgag gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4441 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4501 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4561 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4621 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4681 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcaacgcg tgcggccgcg
     4741 gtacccgggg atcctctaga gtcgaccacc aagggcacca tctctgcttg ggccaccccg
     4801 ttggccgcag ccagctcgct gagagccgtg aacgacaggg cgaacgccag cccgccgacg
     4861 gcgagggttc cgaccgctgc aactcccggt gcaaccttgt cccggtctat tctcttcact
     4921 gcaccagctc caatctggtg tgaatgcccc tcgtctgttc gcgcaggcgg ggggctctat
     4981 tcgtttgtca gcatcgaaag tagccagatc agggatgcgt tgcaaccgcg tatgcccagg
     5041 tcagaagagt cgcacaagag ttgcagaccc ctggaaagaa aaatggccag agggcgaaaa
     5101 caccctctga ccagcggagc gggcgacggg aatcgaaccc gcgtagctag tttggaagaa
     5161 tgggtgtctg ccgaccacat atgggccggt caagataggt ttttaccccc tctcggctgc
     5221 atcctctaag tggaaagaaa ttgcaggtcg tagaagcgcg ttgaagcctg agagttgcac
     5281 aggagttgca acccggtagc cttgttcacg acgagaggag acctagttgg cacgtcgcgg
     5341 atggggatcg ctgaagactc agcgcagcgg gaggatccaa gcctcatacg tcaacccgca
     5401 ggacggtgtg aggtactacg cgctgcagac ctacgacaac aagatggacg ccgaagcctg
     5461 gctcgcgggc gagaagcggc tcatcgagat ggagacctgg acccctccac aggaccgggc
     5521 gaagaag
//
