LOCUS       sequence-345482-        5621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1055:Dendra
FEATURES             Location/Qualifiers
     source          1..5621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     357..376
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        357..375
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1876..1899
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2156..2956
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3081..3669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3570..3589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcgagcgcc agcaccgccg ctctgtgcgg agacctgggc accagccccg ccgccgccag
       61 gagcattgcc gttcccgcca gaaatctaga cggtgaccac aacgcgcccg ctttgatcgg
      121 ggacgtcgaa atattggatc gtcggcaccg tcacggccgt gggaggcggc acgatccgcg
      181 acgtgatgat cggccgcatc cccacggtgc tgcgcagtga gctctacgcc atcccggcgt
      241 tgatctgtgc gttcgcacgc acaggcccgg tgtgagaagg gtctctgacg agcgggagaa
      301 ctccctatca gtgatagagt ttgtcctccc tatcagtgat agataggctt aaggagtaat
      361 acgactcact atagggagaa ggaggccatt atgacgacga tgtggggcgc accgattcac
      421 aagcggtggc ggggctcgcg tctgcgcgat ccgcgtcaag ccgacttcct gaccatggcg
      481 tcgctgaagt gggtcatcgc caaccgggcg ttcacgccct ggtacctggt gcggtactgg
      541 cggctgctca agttcaagct ggccaacccg cacatcatca cccgcggcat ggtgttcctg
      601 ggcaaggacg tggagatcca gtgcacgccc gaactcgcgc agatggagat cggccgctgg
      661 gtgcacatcg gtgacaagaa caccatccgc tgccacgagg gctcgctgcg catcggcgac
      721 aaggtggtgc tgggccgcga caacgtcatc aacacctacc tcgacatcga gctcggtgac
      781 tcggtgctca tggccgactg gtgctacgtc tgcgacttcg accacaagat ggacgacatc
      841 aacgtcccca tcaaggatca gggcatcatc aagggcccgg tgcgtatcgg gcccgacacc
      901 tggatcgccg ccaaggtcac cattctgcgc aacaccctga tcggccgcgg ttgcgtgctc
      961 ggcgcccacg ccgtggtcaa gggggacatc ccggaccact cgatcgcggt cggcgcaccc
     1021 gccaaggtgg tcaagaaccg caagctggcg tgggagacgt cggcggccga gcgcgccgaa
     1081 ctcgccgctg cactcgccga catcgagcgc aagaaggccg tgagcaacaa gaagcttggt
     1141 gcggccgcgg gcaacacgcc tggcatcaat ctgatcaagg aggacatgcg tgtcaaggtg
     1201 catatggagg gcaacgtgaa cggtcacgcc ttcgtgatcg agggggaggg taagggtaag
     1261 ccttacgaag ggacccagac cgccaacctc accgtcaagg agggcgcccc tctcccattc
     1321 tcgtacgata tcctcactac cgcggtgcat tacgggaacc gcgtgttcac caagtacccc
     1381 gaggatatcc ctgactactt caagcagagc ttcccggagg gatactcctg ggagcggacg
     1441 atgaccttcg aagacaaggg tatctgtacg atccgcagcg acatctcgct ggaaggggac
     1501 tgtttcttcc agaacgtccg tttcaagggg accaactttc cgccgaacgg cccggtcatg
     1561 cagaagaaga ccctcaagtg ggagccgagc acggagaagc tgcacgttcg ggacggcctc
     1621 ctggtcggca acatcaacat ggcgctgctg ctggaaggcg gcggccacta cctatgcgac
     1681 ttcaagacca cgtacaaagc gaagaaggtc gtccagctgc ccgacgcgca cttcgtggac
     1741 caccgcatcg aaatcctggg gaacgattcg gactacaaca aggtcaagtt gtacgagcac
     1801 gctgtggcac gctatagccc gctcccgtcg caggtctggt tcgaaggcgg cggcggggag
     1861 aacctgtatt tccaggacta caaggacgac gacgacaagt gatttaaata accccttggg
     1921 gcctctaaac gggtcttgag gggttttttg ttaactagcg tacgatcgaa ttcctgcagc
     1981 tcacggtaac tgatgccgta tttgcagtac cagcgtacgg cccacagaat gatgtcacgc
     2041 tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca tcagcaaaag gggatgataa
     2101 gtttatcacc accgactatt tgcaacagtg ccgttgatcg tgctatgatc gactgatgtc
     2161 atcagcggtg gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc tcatcggtca
     2221 gcttctcaac cttggggtta cccccggcgg tgtgctgctg gtccacagct ccttccgtag
     2281 cgtccggccc ctcgaagatg ggccacttgg actgatcgag gccctgcgtg ctacgctggg
     2341 tccgggaggg acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc cgttcgatcc
     2401 tgccacgtcg cccgttacac cggaccttgg agttgtctct gacacattct ggcgcctgcc
     2461 aaatgtaaag cgcagcgccc atccatttgc ctttgcggca gcggggccac aggcagagca
     2521 gatcatctct gatccattgc ccctgccacc ttactcgcct gcaagcccgg tcgcccgtgt
     2581 ccatgaactc gatgggcagg tacttctcct cggcgtggga cacgatgcca acacgacgct
     2641 gcatcttgcc gagttgatgg caaaggttcc ctatggggtg ccgagacact gcaccattct
     2701 tcaggatggc aagttggtac gcgtcgatta tctcgagaat gaccactgct gtgagcgctt
     2761 tgccttggcg gacaggtggc tcaaggagaa gagccttcag aaggaaggtc cagtcggtca
     2821 tgcctttgct cggttgatcc gctcccgcga cattgtggcg acagccctgg gtcaactggg
     2881 ccgagatccg ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat gcgatgccgc
     2941 tcgccagtcg attggctgag ctcatgagcg gagaacgaga tgacgttgga ggggcaaggt
     3001 cgcgctgatt gctggggcaa cacgggggat ccactagttc catgagcgtc agaccccgta
     3061 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3121 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3181 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     3241 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3301 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3361 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3421 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3481 agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3541 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3601 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3661 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3721 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3781 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg cggccgcggt
     3841 acccggggat cctctagagt cgaccaccaa gggcaccatc tctgcttggg ccaccccgtt
     3901 ggccgcagcc agctcgctga gagccgtgaa cgacagggcg aacgccagcc cgccgacggc
     3961 gagggttccg accgctgcaa ctcccggtgc aaccttgtcc cggtctattc tcttcactgc
     4021 accagctcca atctggtgtg aatgcccctc gtctgttcgc gcaggcgggg ggctctattc
     4081 gtttgtcagc atcgaaagta gccagatcag ggatgcgttg caaccgcgta tgcccaggtc
     4141 agaagagtcg cacaagagtt gcagacccct ggaaagaaaa atggccagag ggcgaaaaca
     4201 ccctctgacc agcggagcgg gcgacgggaa tcgaacccgc gtagctagtt tggaagaatg
     4261 ggtgtctgcc gaccacatat gggccggtca agataggttt ttaccccctc tcggctgcat
     4321 cctctaagtg gaaagaaatt gcaggtcgta gaagcgcgtt gaagcctgag agttgcacag
     4381 gagttgcaac ccggtagcct tgttcacgac gagaggagac ctagttggca cgtcgcggat
     4441 ggggatcgct gaagactcag cgcagcggga ggatccaagc ctcatacgtc aacccgcagg
     4501 acggtgtgag gtactacgcg ctgcagacct acgacaacaa gatggacgcc gaagcctggc
     4561 tcgcgggcga gaagcggctc atcgagatgg agacctggac ccctccacag gaccgggcga
     4621 agaaggcagc cgccagcgcc atcacgctgg aggagtacac ccggaagtgg ctcgtggagc
     4681 gcgacctcgc agacggcacc agggatctgt acagcgggca cgcggagcgc cgcatctacc
     4741 cggtgctagg tgaagtggcg gtcacagaga tgacgccagc tctggtgcgt gcgtggtggg
     4801 ccgggatggg taggaagcac ccgactgccc gccggcatgc ctacaacgtc ctccgggcgg
     4861 tgatgaacac agcggtcgag gacaagctga tcgcagagaa cccgtgccgg atcgagcaga
     4921 aggcagccga tgagcgcgac gtagaggcgc tgacgcctga ggagctggac atcgtcgccg
     4981 ctgagatctt cgagcactac cggatcgcgg catacatcct ggcgtggacg agcctccggt
     5041 tcggagagct gatcgagctt cgccgcaagg acatcgtgga cgacggcatg acgatgaagc
     5101 tccgggtgcg ccgtggcgct tcccgcgtgg ggaacaagat cgtcgttggc aacgccaaga
     5161 ccgtccggtc gaagcgtcct gtgacggttc cgcctcacgt cgcggagatg atccgagcgc
     5221 acatgaagga ccgtacgaag atgaacaagg gccccgaggc attcctggtg accacgacgc
     5281 agggcaaccg gctgtcgaag tccgcgttca ccaagtcgct gaagcgtggc tacgccaaga
     5341 tcggtcggcc ggaactccgc atccacgacc tccgcgctgt cggcgctacg ttcgccgctc
     5401 aggcaggtgc gacgaccaag gagctgatgg cccgtctcgg tcacacgact cctaggatgg
     5461 cgatgaagta ccagatggcg tctgaggccc gcgacgaggc tatcgctgag gcgatgtcca
     5521 agctggccaa gacctcctga aacgcaaaaa gcccccctcc caaggacact gagtcctaaa
     5581 gaggggggtt tcttgtcagt acgcgaagaa ccacgcctgg c
//
