LOCUS       sequence-345485-        9377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression and purification, combined with SAE1 to form 
            the E1 enzyme heterodimer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Peeples W, Rosen MK
  TITLE     Mechanistic dissection of increased enzymatic rate in a 
            phase-separated compartment.
  JOURNAL   Nat Chem Biol. 2021 Jun;17(6):693-702. doi: 
            10.1038/s41589-021-00801-x. Epub 2021 May 25.
  PUBMED    34035521
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00801-x"; journalName: "Nat Chem Biol"; date: 
            "2021-06"; volume: "17"; issue: "6"; pages: "693-702"
COMMENT     Sequence Label: SAE2-mGarnet
FEATURES             Location/Qualifiers
     source          1..9377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(734..739,740..755,756..762)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    770..786
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     775..797
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             856..1956
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1813..1822
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1930..1953
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2005..2016
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2029..2049
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(4773..4790)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4773..4789)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4782..4804)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4968..4985)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      5138..5224
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5316..5343
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5362..5453
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5454..5522,5523..6314)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5672..5691)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(6356..6869)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6551..6572)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6763..6782
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6980..7568
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7469..7488
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7722..7739
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7845..7864)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8005..8027
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8021..8212)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        8708..8785
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             join(8786..9377,1..491)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8805..8824)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag
       61 gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg
      121 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta
      181 ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag
      241 acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc
      301 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
      361 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct
      421 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      481 gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc acaattctca
      541 tgtttgacag cttatcatcg actgcacggt gcaccaatgc ttctggcgtc aggcagccat
      601 cggaagctgt ggtatggctg tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg
      661 cgcactcccg ttctggataa tgttttttgc gccgacatca taacggttct ggcaaatatt
      721 ctgaaatgag ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga
      781 taacaatttc acacaggaaa cagccagtcc gtttaggtgt tttcacgagc acttcaccaa
      841 caaggaccat agattatgaa aactgaagaa ggtaaactgg taatctggat taacggcgat
      901 aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac cggaattaaa
      961 gtcaccgttg agcatccgga taaactggaa gagaaattcc cacaggttgc ggcaactggc
     1021 gatggccctg acattatctt ctgggcacac gaccgctttg gtggctacgc tcaatctggc
     1081 ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc gtttacctgg
     1141 gatgccgtac gttacaacgg caagctgatt gcttacccga tcgctgttga agcgttatcg
     1201 ctgatttata acaaagatct gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg
     1261 ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg
     1321 tacttcacct ggccgctgat tgctgctgac gggggttatg cgttcaagta tgaaaacggc
     1381 aagtacgaca ttaaagacgt gggcgtggat aacgctggcg cgaaagcggg tctgaccttc
     1441 ctggttgacc tgattaaaaa caaacacatg aatgcagaca ccgattactc catcgcagaa
     1501 gctgccttta ataaaggcga aacagcgatg accatcaacg gcccgtgggc atggtccaac
     1561 atcgacacca gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa gggtcaacca
     1621 tccaaaccgt tcgttggcgt gctgagcgca ggtattaacg ccgccagtcc gaacaaagag
     1681 ctggcaaaag agttcctcga aaactatctg ctgactgatg aaggtctgga agcggttaat
     1741 aaagacaaac cgctgggtgc cgtagcgctg aagtcttacg aggaagagtt ggcgaaagat
     1801 ccacgtattg ccgccaccat ggaaaacgcc cagaaaggtg aaatcatgcc gaacatcccg
     1861 cagatgtccg ctttctggta tgccgtgcgt actgcggtga tcaacgccgc cagcggtcgt
     1921 cagactgtcg atgaagccct gaaagacgcg cagactaatt cgagctcgaa caacaacaac
     1981 aataacaata acaacaacct cgggatcgag ggaaggattt cagaatttga aaacctgtat
     2041 tttcagggcg aattctctgg cggctctggc ggctccggtg gttccatggc actgtcgcgg
     2101 gggctgcccc gggagctggc tgaggcggtg gccgggggcc gggtgctggt ggtgggggcg
     2161 ggcggcatcg gctgcgagct cctcaagaat ctcgtgctca ccggtttctc ccacatcgac
     2221 ctgattgatc tggatactat tgatgtaagc aacctcaaca gacagttttt gtttcaaaag
     2281 aaacatgttg gaagatcaaa ggcacaggtt gccaaggaaa gtgtactgca gttttacccg
     2341 aaagctaata tcgttgccta ccatgacagc atcatgaacc ctgactataa tgtggaattt
     2401 ttccgacagt ttatactggt tatgaatgct ttagataaca gagctgcccg aaaccatgtt
     2461 aatagaatgt gcctggcagc tgatgttcct cttattgaaa gtggaacagc tgggtatctt
     2521 ggacaagtaa ctactatcaa aaagggtgtg accgagtgtt atgagtgtca tcctaagccg
     2581 acccagagaa cctttcctgg ctgtacaatt cgtaacacac cttcagaacc tatacattgc
     2641 atcgtttggg caaagtactt gttcaaccag ttgtttgggg aagaagatgc tgatcaagaa
     2701 gtatctcctg acagagctga ccctgaagct gcctgggaac caacggaagc cgaagccaga
     2761 gctagagcat ctaatgaaga tggtgacatt aaacgtattt ctactaagga atgggctaaa
     2821 tcaactggat atgatccagt taaacttttt accaagcttt ttaaagatga catcaggtat
     2881 ctgttgacaa tggacaaact atggcggaaa aggaaacctc cagttccgtt ggactgggct
     2941 gaagtacaaa gtcaaggaga agaaacgaat gcatcagatc aacagaatga accccagtta
     3001 ggcctgaaag accagcaggt tctagatgta aagagctatg cacgtctttt ttcaaagagc
     3061 atcgagactt tgagagttca tttagcagaa aagggggatg gagctgagct catatgggat
     3121 aaggatgacc catctgcaat ggattttgtc acctctgctg caaacctcag gatgcatatt
     3181 ttcagtatga atatgaagag tagatttgat atcaaatcaa tggcagggaa cattattcct
     3241 gctattgcta ctactaatgc agtaattgct gggttgatag tattggaagg attgaagatt
     3301 ttatcaggaa aaatagacca gtgcagaaca atttttttga ataaacaacc aaacccaaga
     3361 aagaagcttc ttgtgccttg tgcactggat cctcccaacc ccaattgtta tgtatgtgcc
     3421 agcaagccag aggtgactgt gcggctgaat gtccataaag tgactgttct caccttacaa
     3481 gacaagatag tgaaagaaaa atttgctatg gtagcaccag atgtccaaat tgaagatggg
     3541 aaaggaacaa tcctaatatc ttccgaagag ggagagacgg aagctaataa tcacaagaag
     3601 ttgtcagaat ttggaattag aaatggcagc cggcttcaag cagatgactt cctccaggac
     3661 tatactttat tgatcaacat ccttcatagt gaagacctag gaaaggacgt tgaatttgaa
     3721 gttgttggtg atgccccgga aaaagtgggg cccaaacaag ctgaagatgc tgccaaaagc
     3781 ataaccaatg gcagtgatga tggagctcag ccctccacct ccacagctca agagcaagat
     3841 gacgttctca tagttgattc ggatgaagaa gattcttcaa ataatgccga cgtcagtgaa
     3901 gaagagagaa gccgcaagag gaaattagat gagaaagaga atctcagtgc aaagaggtca
     3961 cgtatagaac agaaggaaga gcttgatgat gtcatagcat tagatctcga cctgagaagc
     4021 cgtgctcagg cttcgaactc tgcagttgac ggcaccaaca gccttatcaa ggagaacatg
     4081 cgtatgaaag ttgtattaga aggctccgtc aatgggcacc agtttaaatg cacgggcgag
     4141 ggcgagggta acccctatat gggaactcag acgatgcgca ttaaagttat tgaaggcgga
     4201 ccgttacctt ttgcttttga catccttgca acttcattca tgtatggcag taagactttc
     4261 atcaaatatc caaaaggaat ccccgatttc tttaagcagt cattccccga aggcttcacg
     4321 tgggagcgtg tgactcgtta cgaggatggt ggggttatca cggtaatgca agacacctca
     4381 cttgaggacg gctgcttggt ttatcacgcg caagttcgcg gcgtcaattt tccgagcaac
     4441 ggggcagtca tgcaaaagaa aacaaagggg tgggaaccca acactgagat gatgtacccc
     4501 gcagatgggg ggttacgcgg ctataatcac atggcattga aggttgatgg cgggggccac
     4561 ttatcatgta gcctggtcac tacataccgt agtaagaaaa ccgtcggaaa tatcaaaatg
     4621 ccagggattc acgccgttga ccgtcgttta gaacgcttgg aagagtcgga caatgagatg
     4681 ttcgtggtcc aacgcgagca tgcagtggct aaatttgccg ggctgggtgg tggataagga
     4741 tcctctagag tcgacctgca ggcaagcttg gcactggccg tcgttttaca acgtcgtgac
     4801 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     4861 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     4921 ggcgaatggc agcttggctg ttttggcgga tgagataaga ttttcagcct gatacagatt
     4981 aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg
     5041 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
     5101 gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
     5161 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     5221 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
     5281 acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct
     5341 ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc
     5401 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5461 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5521 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5581 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5641 gtttcccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     5701 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5761 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5821 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5881 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5941 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6001 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6061 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6121 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6181 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6241 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6301 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttacccc
     6361 ggttgataat cagaaaagcc ccaaaaacag gaagattgta taagcaaata tttaaattgt
     6421 aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     6481 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagcccg agatagggtt
     6541 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     6601 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac ccaaatcaag
     6661 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     6721 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     6781 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     6841 cgcgcttaat gcgccgctac agggcgcgta aaaggatcta ggtgaagatc ctttttgata
     6901 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6961 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7021 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7081 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     7141 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7201 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7261 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7321 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7381 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7441 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7501 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7561 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7621 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7681 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7741 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     7801 gcatgtggtg cactctcagt acaccgcatg tggtgcactc tcagtacaat ctgctctgat
     7861 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     7921 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     7981 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     8041 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtgc agcgattcac
     8101 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct
     8161 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct
     8221 ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc
     8281 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac
     8341 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct
     8401 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc
     8461 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac
     8521 cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg
     8581 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc
     8641 gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg
     8701 aaattccgac accatcgaat ggtgcaaaac ctttcgcggt atggcatgat agcgcccgga
     8761 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta
     8821 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc
     8881 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt
     8941 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc
     9001 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc
     9061 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca
     9121 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga
     9181 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga
     9241 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga
     9301 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt
     9361 ctcggcgcgt ctgcgtc
//
