LOCUS       sequence-345491-       11985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11985)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 11985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-R1441C
FEATURES             Location/Qualifiers
     source          1..11985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        554..572
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      604..613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..642
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             658..687
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    706..730
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8347..8371)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8420..8439)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8421..8439)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    8713..8834
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8750..8769)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8804..8823
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8841..9296)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8978..8999)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9190..9209
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9323..9427
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9429..9786
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9429..9620
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9452..9472)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9637..9772
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9821..10615
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9821..10615
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9875..9894)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10485..10504
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10803..10822)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10847..10894
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11223..11811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11712..11731
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgatt
      541 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggagctc caccgcggtg
      601 gcggccgcca tggagcagaa actcatctct gaagaggatc tgagcccggg cggatccgag
      661 cagaaactca tctctgaaga ggatctgctg caggaattcg atatcacaag tttgtacaaa
      721 aaagcaggct ccgaattcgc ccttatggct agtggcagct gtcaggggtg cgaagaggac
      781 gaggaaactc tgaagaagtt gatagtcagg ctgaacaatg tccaggaagg aaaacagata
      841 gaaacgctgg tccaaatcct ggaggatctg ctggtgttca cgtactccga gcacgcctcc
      901 aagttatttc aaggcaaaaa tatccatgtg cctctgttga tcgtcttgga ctcctatatg
      961 agagtcgcga gtgtgcagca ggtgggttgg tcacttctgt gcaaattaat agaagtctgt
     1021 ccaggtacaa tgcaaagctt aatgggaccc caggatgttg gaaatgattg ggaagtcctt
     1081 ggtgttcacc aattgattct taaaatgcta acagttcata atgccagtgt aaacttgtca
     1141 gtgattggac tgaagacctt agatctcctc ctaacttcag gtaaaatcac cttgctgata
     1201 ttggatgaag aaagtgatat tttcatgtta atttttgatg ccatgcactc atttccagcc
     1261 aatgatgaag tccagaaact tggatgcaaa gctttacatg tgctgtttga gagagtctca
     1321 gaggagcaac tgactgaatt tgttgagaac aaagattata tgatattgtt aagtgcgtta
     1381 acaaatttta aagatgaaga ggaaattgtg cttcatgtgc tgcattgttt acattcccta
     1441 gcgattcctt gcaataatgt ggaagtcctc atgagtggca atgtcaggtg ttataatatt
     1501 gtggtggaag ctatgaaagc attccctatg agtgaaagaa ttcaagaagt gagttgctgt
     1561 ttgctccata ggcttacatt aggtaatttt ttcaatatcc tggtattaaa cgaagtccat
     1621 gagtttgtgg tgaaagctgt gcagcagtac ccagagaatg cagcattgca gatctcagcg
     1681 ctcagctgtt tggccctcct cactgagact attttcttaa atcaagattt agaggaaaag
     1741 aatgagaatc aagagaatga tgatgagggg gaagaagata aattgttttg gctggaagcc
     1801 tgttacaaag cattaacgtg gcatagaaag aacaagcacg tgcaggaggc cgcatgctgg
     1861 gcactaaata atctccttat gtaccaaaac agtttacatg agaagattgg agatgaagat
     1921 ggccatttcc cagctcatag ggaagtgatg ctctccatgc tgatgcattc ttcatcaaag
     1981 gaagttttcc aggcatctgc gaatgcattg tcaactctct tagaacaaaa tgttaatttc
     2041 agaaaaatac tgttatcaaa aggaatacac ctgaatgttt tggagttaat gcagaagcat
     2101 atacattctc ccgaagtggc tgaaagtggc tgtaaaatgc taaatcatct ttttgaagga
     2161 agcaacactt ccctggatat aatggcagca gtggtcccca aaatactaac agttatgaaa
     2221 cgtcatgaga catcattacc agtgcagctg gaggcgcttc gagctatttt acattttata
     2281 gtgcctggca tgccagaaga atccagggag gatacagaat ttcatcataa gctaaatatg
     2341 gttaaaaaac agtgtttcaa gaatgatatt cacaaactgg tcctagcagc tttgaacagg
     2401 ttcattggaa atcctgggat tcagaaatgt ggattaaaag taatttcttc tattgtacat
     2461 tttcctgatg cattagagat gttatccctg gaaggtgcta tggattcagt gcttcacaca
     2521 ctgcagatgt atccagatga ccaagaaatt cagtgtctgg gtttaagtct tataggatac
     2581 ttgattacaa agaagaatgt gttcatagga actggacatc tgctggcaaa aattctggtt
     2641 tccagcttat accgatttaa ggatgttgct gaaatacaga ctaaaggatt tcagacaatc
     2701 ttagcaatcc tcaaattgtc agcatctttt tctaagctgc tggtgcatca ttcatttgac
     2761 ttagtaatat tccatcaaat gtcttccaat atcatggaac aaaaggatca acagtttcta
     2821 aacctctgtt gcaagtgttt tgcaaaagta gctatggatg attacttaaa aaatgtgatg
     2881 ctagagagag cgtgtgatca gaataacagc atcatggttg aatgcttgct tctattggga
     2941 gcagatgcca atcaagcaaa ggagggatct tctttaattt gtcaggtatg tgagaaagag
     3001 agcagtccca aattggtgga actcttactg aatagtggat ctcgtgaaca agatgtacga
     3061 aaagcgttga cgataagcat tgggaaaggt gacagccaga tcatcagctt gctcttaagg
     3121 aggctggccc tggatgttgc caacaatagc atttgccttg gaggattttg tataggaaaa
     3181 gttgaacctt cttggcttgg tcctttattt ccagataaga cttctaattt aaggaaacaa
     3241 acaaatatag catctacact agcaagaatg gtgatcagat atcagatgaa aagtgctgtg
     3301 gaagaaggaa cagcctcagg cagcgatgga aatttttctg aagatgtgct gtctaaattt
     3361 gatgaatgga cctttattcc tgactcttct atggacagtg tgtttgctca aagtgatgac
     3421 ctggatagtg aaggaagtga aggctcattt cttgtgaaaa agaaatctaa ttcaattagt
     3481 gtaggagaat tttaccgaga tgccgtatta cagcgttgct caccaaattt gcaaagacat
     3541 tccaattcct tggggcccat ttttgatcat gaagatttac tgaagcgaaa aagaaaaata
     3601 ttatcttcag atgattcact caggtcatca aaacttcaat cccatatgag gcattcagac
     3661 agcatttctt ctctggcttc tgagagagaa tatattacat cactagacct ttcagcaaat
     3721 gaactaagag atattgatgc cctaagccag aaatgctgta taagtgttca tttggagcat
     3781 cttgaaaagc tggagcttca ccagaatgca ctcacgagct ttccacaaca gctatgtgaa
     3841 actctgaaga gtttgacaca tttggacttg cacagtaata aatttacatc atttccttct
     3901 tatttgttga aaatgagttg tattgctaat cttgatgtct ctcgaaatga cattggaccc
     3961 tcagtggttt tagatcctac agtgaaatgt ccaactctga aacagtttaa cctgtcatat
     4021 aaccagctgt cttttgtacc tgagaacctc actgatgtgg tagagaaact ggagcagctc
     4081 attttagaag gaaataaaat atcagggata tgctccccct tgagactgaa ggaactgaag
     4141 attttaaacc ttagtaagaa ccacatttca tccctatcag agaactttct tgaggcttgt
     4201 cctaaagtgg agagtttcag tgccagaatg aattttcttg ctgctatgcc tttcttgcct
     4261 ccttctatga caatcctaaa attatctcag aacaaatttt cctgtattcc agaagcaatt
     4321 ttaaatcttc cacacttgcg gtctttagat atgagcagca atgatattca gtacctacca
     4381 ggtcccgcac actggaaatc tttgaactta agggaactct tatttagcca taatcagatc
     4441 agcatcttgg acttgagtga aaaagcatat ttatggtcta gagtagagaa actgcatctt
     4501 tctcacaata aactgaaaga gattcctcct gagattggct gtcttgaaaa tctgacatct
     4561 ctggatgtca gttacaactt ggaactaaga tcctttccca atgaaatggg gaaattaagc
     4621 aaaatatggg atcttccttt ggatgaactg catcttaact ttgattttaa acatatagga
     4681 tgtaaagcca aagacatcat aaggtttctt caacagcgat taaaaaaggc tgtgccttat
     4741 aaccgaatga aacttatgat tgtgggaaat actgggagtg gtaaaaccac cttattgcag
     4801 caattaatga aaaccaagaa atcagatctt ggaatgcaaa gtgccacagt tggcatagat
     4861 gtgaaagact ggcctatcca aataagagac aaaagaaaga gagatctcgt cctaaatgtg
     4921 tgggattttg caggtcgtga ggaattctat agtactcatc cccattttat gacgcagcga
     4981 gcattgtacc ttgctgtcta tgacctcagc aagggacagg ctgaagttga tgccatgaag
     5041 ccttggctct tcaatataaa ggcttgcgct tcttcttccc ctgtgattct cgttggcaca
     5101 catttggatg tttctgatga gaagcaacgc aaagcctgca tgagtaaaat caccaaggaa
     5161 ctcctgaata agcgagggtt ccctgccata cgagattacc actttgtgaa tgccaccgag
     5221 gaatctgatg ctttggcaaa acttcggaaa accatcataa acgagagcct taatttcaag
     5281 atccgagatc agcttgttgt tggacagctg attccagact gctatgtaga acttgaaaaa
     5341 atcattttat cggagcgtaa aaatgtgcca attgaatttc ccgtaattga ccggaaacga
     5401 ttattacaac tagtgagaga aaatcagctg cagttagatg aaaatgagct tcctcacgca
     5461 gttcactttc taaatgaatc aggagtcctt cttcattttc aagacccagc actgcagtta
     5521 agtgacttgt actttgtgga acccaagtgg ctttgtaaaa tcatggcaca gattttgaca
     5581 gtgaaagtgg aaggttgtcc aaaacaccct aagggaatta tttcgcgtag agatgtggaa
     5641 aaatttcttt caaaaaaaag gaaatttcca aagaactaca tgtcacagta ttttaagctc
     5701 ctagaaaaat tccagattgc tttgccaata ggagaagaat atttgctggt tccaagcagt
     5761 ttgtctgacc acaggcctgt gatagagctt ccccattgtg agaactctga aattatcatc
     5821 cgactatatg aaatgcctta ttttccaatg ggattttggt caagattaat caatcgatta
     5881 cttgagattt caccttacat gctttcaggg agagaacgag cacttcgccc aaacagaatg
     5941 tattggcgac aaggcattta cttaaattgg tctcctgaag cttattgtct ggtaggatct
     6001 gaagtcttag acaatcatcc agagagtttc ttaaaaatta cagttccttc ttgtagaaaa
     6061 ggctgtattc ttttgggcca agttgtggac cacattgatt ctctcatgga agaatggttt
     6121 cctgggttgc tggagattga tatttgtggt gaaggagaaa ctctgttgaa gaaatgggca
     6181 ttatatagtt ttaatgatgg cgaagaacat caaaaaatct tacttgatga cttgatgaag
     6241 aaagcagagg aaggagatct cttagtaaat ccagatcaac caaggctcac cattccaata
     6301 tctcagattg cccctgactt gattttggct gacctgccta gaaatattat gttgaataat
     6361 gatgagttgg aatttgaaca agctccagag tttctcctag gtgatggcag ttttggatca
     6421 gtttaccgag cagcctatga aggagaagaa gtggctgtga agatttttaa taaacataca
     6481 tcactcaggc tgttaagaca agagcttgtg gtgctttgcc acctccacca ccccagtttg
     6541 atatctttgc tggcagctgg gattcgtccc cggatgttgg tgatggagtt agcctccaag
     6601 ggttccttgg atcgcctgct tcagcaggac aaagccagcc tcactagaac cctacagcac
     6661 aggatcgcac tccacgtagc tgatggtttg agatacctcc actcagccat gattatatac
     6721 cgagacctga aaccccacaa tgtgctgctt ttcacactgt atcccaatgc tgccatcatc
     6781 gcaaagattg ctgactacgg cattgctcag tactgctgta gaatggggat aaaaacatca
     6841 gagggcacac cagggtttcg tgcacctgaa gttgccagag gaaatgtcat ttataaccaa
     6901 caggctgatg tttattcatt tggtttacta ctctatgaca ttttgacaac tggaggtaga
     6961 atagtagagg gtttgaagtt tccaaatgag tttgatgaat tagaaataca aggaaaatta
     7021 cctgatccag ttaaagaata tggttgtgcc ccatggccta tggttgagaa attaattaaa
     7081 cagtgtttga aagaaaatcc tcaagaaagg cctacttctg cccaggtctt tgacattttg
     7141 aattcagctg aattagtctg tctgacgaga cgcattttat tacctaaaaa cgtaattgtt
     7201 gaatgcatgg ttgctacaca tcacaacagc aggaatgcaa gcatttggct gggctgtggg
     7261 cacaccgaca gaggacagct ctcatttctt gacttaaata ctgaaggata cacttctgag
     7321 gaagttgctg atagtagaat attgtgctta gccttggtgc atcttcctgt tgaaaaggaa
     7381 agctggattg tgtctgggac acagtctggt actctcctgg tcatcaatac cgaagatggg
     7441 aaaaagagac ataccctaga aaagatgact gattctgtca cttgtttgta ttgcaattcc
     7501 ttttccaagc aaagcaaaca aaaaaatttt cttttggttg gaaccgctga tggcaagtta
     7561 gcaatttttg aagataagac tgttaagctt aaaggagctg ctcctttgaa gatactaaat
     7621 ataggaaatg tcagtactcc attgatgtgt ttgagtgaat ccacaaattc aacggaaaga
     7681 aatgtaatgt ggggaggatg tggcacaaag attttctcct tttctaatga tttcaccatt
     7741 cagaaactca ttgagacaag aacaagccaa ctgttttctt atgcagcttt cagtgattcc
     7801 aacatcataa cagtggtggt agacactgct ctctatattg ctaagcaaaa tagccctgtt
     7861 gtggaagtgt gggataagaa aactgaaaaa ctctgtggac taatagactg cgtgcacttt
     7921 ttaagggagg taatggtaaa agaaaacaag gaatcaaaac acaaaatgtc ttattctggg
     7981 agagtgaaaa ccctctgcct tcagaagaac actgctcttt ggataggaac tggaggaggc
     8041 catattttac tcctggatct ttcaactcgt cgacttatac gtgtaattta caacttttgt
     8101 aattcggtca gagtcatgat gacagcacag ctaggaagcc ttaaaaatgt catgctggta
     8161 ttgggctaca accggaaaaa tactgaaggt acacaaaagc agaaagagat acaatcttgc
     8221 ttgaccgttt gggacatcaa tcttccacat gaagtgcaaa atttagaaaa acacattgaa
     8281 gtgagaaaag aattagctga aaaaatgaga cgaacatctg ttgagtaaga gagaagggcg
     8341 aattcgaccc agctttcttg tacaaagtgg tgatcggtac cttaattaat taaggtacca
     8401 ggtaagtgta cccaattcgc cctatagtga gtcgtattac aattcactcg atcgcccttc
     8461 ccaacagttg cgcagcctga atggcgaatg gagatccaat ttttaagtgt ataatgtgtt
     8521 aaactactga ttctaattgt ttgtgtattt tagattcaca gtcccaaggc tcatttcagg
     8581 cccctcagtc ctcacagtct gttcatgatc ataatcagcc ataccacatt tgtagaggtt
     8641 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     8701 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8761 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8821 atcaatgtat cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     8881 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     8941 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     9001 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     9061 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     9121 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     9181 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     9241 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     9301 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     9361 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     9421 gaagaatcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     9481 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     9541 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9601 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9661 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9721 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9781 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     9841 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     9901 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     9961 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
    10021 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
    10081 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
    10141 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
    10201 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
    10261 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gaacatcagg ggctcgcgcc
    10321 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
    10381 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
    10441 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
    10501 tattgctgaa gaacttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
    10561 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
    10621 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
    10681 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
    10741 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
    10801 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
    10861 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
    10921 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
    10981 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
    11041 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
    11101 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    11161 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    11221 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    11281 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    11341 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    11401 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    11461 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    11521 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    11581 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    11641 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    11701 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    11761 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    11821 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    11881 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcattagtt attaatagta
    11941 atcaattacg gggtcattag ttcatagccc atatatggag ttccg
//
