LOCUS       sequence-345496-        4983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0938:Dendra
FEATURES             Location/Qualifiers
     source          1..4983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     360..379
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        360..378
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1936..1959
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2216..3016
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3141..3729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3630..3649
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccgcgagc gccagcaccg ccgctctgtg cggagacctg ggcaccagcc ccgccgccgc
       61 caggagcatt gccgttcccg ccagaaatct agacggtgac cacaacgcgc ccgctttgat
      121 cggggacgtc gaaatattgg atcgtcggca ccgtcacggc cgtgggaggc ggcacgatcc
      181 gcgacgtgat gatcggccgc atccccacgg tgctgcgcag tgagctctac gccatcccgg
      241 cgttgatctg tgcgttcgca cgcacaggcc cggtgtgaga agggtctctg acgagcggga
      301 gaactcccta tcagtgatag agtttgtcct ccctatcagt gatagatagg cttaaggagt
      361 aatacgactc actataggga gaaggaggcc attatgacac gtgtgcatcc tggtgaggtt
      421 gaactcgact tcgcccgtga gtgggtggag ttctacgacc ccgatgacgc ggaccatctc
      481 atcgcggcgg acatgacctg gctgctgtcc cgctggacgt gcgtgttcgg caccccggcg
      541 tgccagggca ccgtggaggg ccgccccgac gacggttgct gctcgcacgg cgcgttcctg
      601 tccgacgacg acgaccgggc ccggctcgac gacgccgtca agcagctcac cgacgaggac
      661 tggcagttcc gcgagaaggg cctgggcaag aagggctacc tcgaggacga cgagtacgac
      721 ggcaagccga acctgcgtac ccggaagtac aagggcgcct gcatctttct gaaccgcccc
      781 ggcttcccga cgggcatcgg gtgcgcgctg cacagcaagg cgctcaagct cggtgtcgag
      841 ccgtggacca tgaagcccga cgtgtgctgg cagctgccga tccggcgcag ccaggactgg
      901 gtcgaacggc ccgacggcac gcagatcctc aagacggtca tcaccgagta cgaccgccgc
      961 gggtggggcg agggcggtgc cgacctgcac tggtactgca cgggcgatcc gaacgcacac
     1021 gtgggcagcc gaccggtgtg ggagtcctac gcacccgagc tgaccgccct gctgggcgag
     1081 aaggcctacg ccgagctggc ggcaatctgc aagcgccgca gccgtttagg gctggtcgcc
     1141 gtccatccgg cgacgcgact ggccgagagc gggacatccg gcccgcagcg gaagcttggt
     1201 gcggccgcgg gcaacacgcc tggcatcaat ctgatcaagg aggacatgcg tgtcaaggtg
     1261 catatggagg gcaacgtgaa cggtcacgcc ttcgtgatcg agggggaggg taagggtaag
     1321 ccttacgaag ggacccagac cgccaacctc accgtcaagg agggcgcccc tctcccattc
     1381 tcgtacgata tcctcactac cgcggtgcat tacgggaacc gcgtgttcac caagtacccc
     1441 gaggatatcc ctgactactt caagcagagc ttcccggagg gatactcctg ggagcggacg
     1501 atgaccttcg aagacaaggg tatctgtacg atccgcagcg acatctcgct ggaaggggac
     1561 tgtttcttcc agaacgtccg tttcaagggg accaactttc cgccgaacgg cccggtcatg
     1621 cagaagaaga ccctcaagtg ggagccgagc acggagaagc tgcacgttcg ggacggcctc
     1681 ctggtcggca acatcaacat ggcgctgctg ctggaaggcg gcggccacta cctatgcgac
     1741 ttcaagacca cgtacaaagc gaagaaggtc gtccagctgc ccgacgcgca cttcgtggac
     1801 caccgcatcg aaatcctggg gaacgattcg gactacaaca aggtcaagtt gtacgagcac
     1861 gctgtggcac gctatagccc gctcccgtcg caggtctggt tcgaaggcgg cggcggggag
     1921 aacctgtatt tccaggacta caaggacgac gacgacaagt gatttaaata accccttggg
     1981 gcctctaaac gggtcttgag gggttttttg ttaactagcg tacgatcgaa ttcctgcagc
     2041 tcacggtaac tgatgccgta tttgcagtac cagcgtacgg cccacagaat gatgtcacgc
     2101 tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca tcagcaaaag gggatgataa
     2161 gtttatcacc accgactatt tgcaacagtg ccgttgatcg tgctatgatc gactgatgtc
     2221 atcagcggtg gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc tcatcggtca
     2281 gcttctcaac cttggggtta cccccggcgg tgtgctgctg gtccacagct ccttccgtag
     2341 cgtccggccc ctcgaagatg ggccacttgg actgatcgag gccctgcgtg ctacgctggg
     2401 tccgggaggg acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc cgttcgatcc
     2461 tgccacgtcg cccgttacac cggaccttgg agttgtctct gacacattct ggcgcctgcc
     2521 aaatgtaaag cgcagcgccc atccatttgc ctttgcggca gcggggccac aggcagagca
     2581 gatcatctct gatccattgc ccctgccacc ttactcgcct gcaagcccgg tcgcccgtgt
     2641 ccatgaactc gatgggcagg tacttctcct cggcgtggga cacgatgcca acacgacgct
     2701 gcatcttgcc gagttgatgg caaaggttcc ctatggggtg ccgagacact gcaccattct
     2761 tcaggatggc aagttggtac gcgtcgatta tctcgagaat gaccactgct gtgagcgctt
     2821 tgccttggcg gacaggtggc tcaaggagaa gagccttcag aaggaaggtc cagtcggtca
     2881 tgcctttgct cggttgatcc gctcccgcga cattgtggcg acagccctgg gtcaactggg
     2941 ccgagatccg ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat gcgatgccgc
     3001 tcgccagtcg attggctgag ctcatgagcg gagaacgaga tgacgttgga ggggcaaggt
     3061 cgcgctgatt gctggggcaa cacgggggat ccactagttc catgagcgtc agaccccgta
     3121 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3181 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3241 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     3301 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3361 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3421 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3481 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3541 agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3601 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3661 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3721 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3781 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3841 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg cggccgcggt
     3901 acccggggat cctctagagt cgaccaccaa gggcaccatc tctgcttggg ccaccccgtt
     3961 ggccgcagcc agctcgctga gagccgtgaa cgacagggcg aacgccagcc cgccgacggc
     4021 gagggttccg accgctgcaa ctcccggtgc aaccttgtcc cggtctattc tcttcactgc
     4081 accagctcca atctggtgtg aatgcccctc gtctgttcgc gcaggcgggg ggctctattc
     4141 gtttgtcagc atcgaaagta gccagatcag ggatgcgttg caaccgcgta tgcccaggtc
     4201 agaagagtcg cacaagagtt gcagacccct ggaaagaaaa atggccagag ggcgaaaaca
     4261 ccctctgacc agcggagcgg gcgacgggaa tcgaacccgc gtagctagtt tggaagaatg
     4321 ggtgtctgcc gaccacatat gggccggtca agataggttt ttaccccctc tcggctgcat
     4381 cctctaagtg gaaagaaatt gcaggtcgta gaagcgcgtt gaagcctgag agttgcacag
     4441 gagttgcaac ccggtagcct tgttcacgac gagaggagac ctagttggca cgtcgcggat
     4501 ggggatcgct gaagactcag cgcagcggga ggatccaagc ctcatacgtc aacccgcagg
     4561 acggtgtgag gtactacgcg ctgcagacct acgacaacaa gatggacgcc gaagcctggc
     4621 tcgcgggcga gaagcggctc atcgagatgg agacctggac ccctccacag gaccgggcga
     4681 agaaggcagc cgccagcgcc atcacgctgg aggagtacac ccggaagtgg ctcgtggagc
     4741 gcgacctcgc agacggcacc agggatctgt acagcgggca cgcggagcgc cgcatctacc
     4801 cggtgctagg tgaagtggcg gtcacagaga tgacgccagc tctggtgcgt gcgtggtggg
     4861 ccgggatggg taggaagcac ccgactgccc gccggcatgc ctacaacgtc ctccgggcgg
     4921 tgatgaacac agcggtcgag gacaagctga tcgcagagaa cccgtgccgg atcgagcaga
     4981 agg
//
