LOCUS       sequence-345497-        6233 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0952:Dendra
FEATURES             Location/Qualifiers
     source          1..6233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     746..765
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        746..764
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2877..2900
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3157..3957
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      4082..4670
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4571..4590
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtacgaagat gaacaagggc cccgaggcat tcctggtgac cacgacgcag ggcaaccggc
       61 tgtcgaagtc cgcgttcacc aagtcgctga agcgtggcta cgccaagatc ggtcggccgg
      121 aactccgcat ccacgacctc cgcgctgtcg gcgctacgtt cgccgctcag gcaggtgcga
      181 cgaccaagga gctgatggcc cgtctcggtc acacgactcc taggatggcg atgaagtacc
      241 agatggcgtc tgaggcccgc gacgaggcta tcgctgaggc gatgtccaag ctggccaaga
      301 cctcctgaaa cgcaaaaagc ccccctccca aggacactga gtcctaaaga ggggggtttc
      361 ttgtcagtac gcgaagaacc acgcctggcc gcgagcgcca gcaccgccgc tctgtgcgga
      421 gacctgggca ccagccccgc cgccgccagg agcattgccg ttcccgccag aaatctagac
      481 ggtgaccaca acgcgcccgc tttgatcggg gacgtcgaaa tattggatcg tcggcaccgt
      541 cacggccgtg ggaggcggca cgatccgcga cgtgatgatc ggccgcatcc ccacggtgct
      601 gcgcagtgag ctctacgcca tcccggcgtt gatctgtgcg ttcgcacgca caggcccggt
      661 gtgagaaggg tctctgacga gcgggagaac tccctatcag tgatagagtt tgtcctccct
      721 atcagtgata gataggctta aggagtaata cgactcacta tagggagaag gaggccatta
      781 tgagcgtgct gctattcggg gtttcgcacc gcagtgcgcc ggtgtctgtg ctcgaacagc
      841 tgagcaccaa cgaagccgag caggcgaaga tcatcgacca ggttctccag tcctcactgg
      901 ttaccgaggc catggtgctt tccacctgca accgcgtcga ggtgtatgcg gtggtcgagg
      961 ccttccacgg cggtctgtcg gtgatcgggc aggtgctcgc tgagcgctcc ggtatgtccc
     1021 tcaatgacct gaccaaatac gcctatgtcc gctacgcgga ggcggccgtc gagcatctgt
     1081 tcgcggtgac cagcggtctg gattccgcgg tcatcggcga gcagcaggtg ctgggacagg
     1141 tgcgtcgcgc gtacgcgacg gccgaggcca accgcaccgt gggccgcaca ctgcacgaac
     1201 tcgcgcagcg cgccctgtcg gtgggcaagc gggtgcactc ggagaccggg atcgacgccg
     1261 caggcgcgtc ggtggtctcg gtggcgctcg gcatggccga gaccaaactc tcgggtgggc
     1321 tcggcggacg caccgcggcc gtggtcggtg cgggcgcgat gggcgcactc gccggtgcgc
     1381 acctggtgcg cgcgggcatc gaacgcgtcc acgtggtcaa ccggtccctg ccgcgcgccg
     1441 agcgcctggc caagaacctc accgagcagg gcgtggtggc cgacgccgtg ggtctggacg
     1501 acatcgcccg ggcactcgtc gacgccgacg tcgtgctcag cagcaccggc gccgtgcgcc
     1561 cggtggtctc gctggccgac gtgcactacg cgctggcaca gcgcaccctg gccggtgccg
     1621 aacaccagat ggtcgtgtgc gacctgggca tgccgcgcga cgtcgatccg gccgtgtcgg
     1681 gtttgccggg cgtgtgggtc gtcgacatgg accgcatcca gcgcgaaccg tccgcacgtg
     1741 cggcagcggt cgacgcggag gccgcgcgca acatcgtggc cgccgaggtg gcgaactacc
     1801 tggcgggcca gcgcatggcc gaggtcaccc cgaccgtgac ggcgctgcgg cagcgcgccg
     1861 ccgacgtcgt cgaggccgaa ctgctgcgcc tggacaaccg gctgcccgga ctggacgcgg
     1921 cgcaccgcga cgaggtcgcc aagaccgtcc gtcgcgtcgt cgacaaactg ctgcacgcgc
     1981 cgacggtgcg tgtgaaacaa ctcgcgagcg cccccggcgg ggacagctac gccgaggcgc
     2041 tgcgggaact gttcgaactc gatccgcaag ccgtcgaagc ggttgcggcc agtgaattgc
     2101 ccttgataac aaccgatctc gataagactg agaagcttgg tgcggccgcg ggcaacacgc
     2161 ctggcatcaa tctgatcaag gaggacatgc gtgtcaaggt gcatatggag ggcaacgtga
     2221 acggtcacgc cttcgtgatc gagggggagg gtaagggtaa gccttacgaa gggacccaga
     2281 ccgccaacct caccgtcaag gagggcgccc ctctcccatt ctcgtacgat atcctcacta
     2341 ccgcggtgca ttacgggaac cgcgtgttca ccaagtaccc cgaggatatc cctgactact
     2401 tcaagcagag cttcccggag ggatactcct gggagcggac gatgaccttc gaagacaagg
     2461 gtatctgtac gatccgcagc gacatctcgc tggaagggga ctgtttcttc cagaacgtcc
     2521 gtttcaaggg gaccaacttt ccgccgaacg gcccggtcat gcagaagaag accctcaagt
     2581 gggagccgag cacggagaag ctgcacgttc gggacggcct cctggtcggc aacatcaaca
     2641 tggcgctgct gctggaaggc ggcggccact acctatgcga cttcaagacc acgtacaaag
     2701 cgaagaaggt cgtccagctg cccgacgcgc acttcgtgga ccaccgcatc gaaatcctgg
     2761 ggaacgattc ggactacaac aaggtcaagt tgtacgagca cgctgtggca cgctatagcc
     2821 cgctcccgtc gcaggtctgg ttcgaaggcg gcggcgggga gaacctgtat ttccaggact
     2881 acaaggacga cgacgacaag tgatttaaat aaccccttgg ggcctctaaa cgggtcttga
     2941 ggggtttttt gttaactagc gtacgatcga attcctgcag ctcacggtaa ctgatgccgt
     3001 atttgcagta ccagcgtacg gcccacagaa tgatgtcacg ctgaaaatgc cggcctttga
     3061 atgggttcat gtgcagctcc atcagcaaaa ggggatgata agtttatcac caccgactat
     3121 ttgcaacagt gccgttgatc gtgctatgat cgactgatgt catcagcggt ggagtgcaat
     3181 gtcgtgcaat acgaatggcg aaaagccgag ctcatcggtc agcttctcaa ccttggggtt
     3241 acccccggcg gtgtgctgct ggtccacagc tccttccgta gcgtccggcc cctcgaagat
     3301 gggccacttg gactgatcga ggccctgcgt gctacgctgg gtccgggagg gacgctcgtc
     3361 atgccctcgt ggtcaggtct ggacgacgag ccgttcgatc ctgccacgtc gcccgttaca
     3421 ccggaccttg gagttgtctc tgacacattc tggcgcctgc caaatgtaaa gcgcagcgcc
     3481 catccatttg cctttgcggc agcggggcca caggcagagc agatcatctc tgatccattg
     3541 cccctgccac cttactcgcc tgcaagcccg gtcgcccgtg tccatgaact cgatgggcag
     3601 gtacttctcc tcggcgtggg acacgatgcc aacacgacgc tgcatcttgc cgagttgatg
     3661 gcaaaggttc cctatggggt gccgagacac tgcaccattc ttcaggatgg caagttggta
     3721 cgcgtcgatt atctcgagaa tgaccactgc tgtgagcgct ttgccttggc ggacaggtgg
     3781 ctcaaggaga agagccttca gaaggaaggt ccagtcggtc atgcctttgc tcggttgatc
     3841 cgctcccgcg acattgtggc gacagccctg ggtcaactgg gccgagatcc gttgatcttc
     3901 ctgcatccgc cagaggcggg atgcgaagaa tgcgatgccg ctcgccagtc gattggctga
     3961 gctcatgagc ggagaacgag atgacgttgg aggggcaagg tcgcgctgat tgctggggca
     4021 acacggggga tccactagtt ccatgagcgt cagaccccgt agaaaagatc aaaggatctt
     4081 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4141 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4201 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     4261 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4321 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4381 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4441 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgagg
     4501 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4561 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4621 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4681 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4741 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4801 gccgcagccg aacgaccgag cgcaacgcgt gcggccgcgg tacccgggga tcctctagag
     4861 tcgaccacca agggcaccat ctctgcttgg gccaccccgt tggccgcagc cagctcgctg
     4921 agagccgtga acgacagggc gaacgccagc ccgccgacgg cgagggttcc gaccgctgca
     4981 actcccggtg caaccttgtc ccggtctatt ctcttcactg caccagctcc aatctggtgt
     5041 gaatgcccct cgtctgttcg cgcaggcggg gggctctatt cgtttgtcag catcgaaagt
     5101 agccagatca gggatgcgtt gcaaccgcgt atgcccaggt cagaagagtc gcacaagagt
     5161 tgcagacccc tggaaagaaa aatggccaga gggcgaaaac accctctgac cagcggagcg
     5221 ggcgacggga atcgaacccg cgtagctagt ttggaagaat gggtgtctgc cgaccacata
     5281 tgggccggtc aagataggtt tttaccccct ctcggctgca tcctctaagt ggaaagaaat
     5341 tgcaggtcgt agaagcgcgt tgaagcctga gagttgcaca ggagttgcaa cccggtagcc
     5401 ttgttcacga cgagaggaga cctagttggc acgtcgcgga tggggatcgc tgaagactca
     5461 gcgcagcggg aggatccaag cctcatacgt caacccgcag gacggtgtga ggtactacgc
     5521 gctgcagacc tacgacaaca agatggacgc cgaagcctgg ctcgcgggcg agaagcggct
     5581 catcgagatg gagacctgga cccctccaca ggaccgggcg aagaaggcag ccgccagcgc
     5641 catcacgctg gaggagtaca cccggaagtg gctcgtggag cgcgacctcg cagacggcac
     5701 cagggatctg tacagcgggc acgcggagcg ccgcatctac ccggtgctag gtgaagtggc
     5761 ggtcacagag atgacgccag ctctggtgcg tgcgtggtgg gccgggatgg gtaggaagca
     5821 cccgactgcc cgccggcatg cctacaacgt cctccgggcg gtgatgaaca cagcggtcga
     5881 ggacaagctg atcgcagaga acccgtgccg gatcgagcag aaggcagccg atgagcgcga
     5941 cgtagaggcg ctgacgcctg aggagctgga catcgtcgcc gctgagatct tcgagcacta
     6001 ccggatcgcg gcatacatcc tggcgtggac gagcctccgg ttcggagagc tgatcgagct
     6061 tcgccgcaag gacatcgtgg acgacggcat gacgatgaag ctccgggtgc gccgtggcgc
     6121 ttcccgcgtg gggaacaaga tcgtcgttgg caacgccaag accgtccggt cgaagcgtcc
     6181 tgtgacggtt ccgcctcacg tcgcggagat gatccgagcg cacatgaagg acc
//
