LOCUS       sequence-345498-        5354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1340:Dendra
FEATURES             Location/Qualifiers
     source          1..5354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1220..1239
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1220..1238
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2472..2495
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2752..3552
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3677..4265
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4166..4185
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggtcacaga gatgacgcca gctctggtgc gtgcgtggtg ggccgggatg ggtaggaagc
       61 acccgactgc ccgccggcat gcctacaacg tcctccgggc ggtgatgaac acagcggtcg
      121 aggacaagct gatcgcagag aacccgtgcc ggatcgagca gaaggcagcc gatgagcgcg
      181 acgtagaggc gctgacgcct gaggagctgg acatcgtcgc cgctgagatc ttcgagcact
      241 accggatcgc ggcatacatc ctggcgtgga cgagcctccg gttcggagag ctgatcgagc
      301 ttcgccgcaa ggacatcgtg gacgacggca tgacgatgaa gctccgggtg cgccgtggcg
      361 cttcccgcgt ggggaacaag atcgtcgttg gcaacgccaa gaccgtccgg tcgaagcgtc
      421 ctgtgacggt tccgcctcac gtcgcggaga tgatccgagc gcacatgaag gaccgtacga
      481 agatgaacaa gggccccgag gcattcctgg tgaccacgac gcagggcaac cggctgtcga
      541 agtccgcgtt caccaagtcg ctgaagcgtg gctacgccaa gatcggtcgg ccggaactcc
      601 gcatccacga cctccgcgct gtcggcgcta cgttcgccgc tcaggcaggt gcgacgacca
      661 aggagctgat ggcccgtctc ggtcacacga ctcctaggat ggcgatgaag taccagatgg
      721 cgtctgaggc ccgcgacgag gctatcgctg aggcgatgtc caagctggcc aagacctcct
      781 gaaacgcaaa aagcccccct cccaaggaca ctgagtccta aagagggggg tttcttgtca
      841 gtacgcgaag aaccacgcct ggccgcgagc gccagcaccg ccgctctgtg cggagacctg
      901 ggcaccagcc ccgccgccgc caggagcatt gccgttcccg ccagaaatct agacggtgac
      961 cacaacgcgc ccgctttgat cggggacgtc gaaatattgg atcgtcggca ccgtcacggc
     1021 cgtgggaggc ggcacgatcc gcgacgtgat gatcggccgc atccccacgg tgctgcgcag
     1081 tgagctctac gccatcccgg cgttgatctg tgcgttcgca cgcacaggcc cggtgtgaga
     1141 agggtctctg acgagcggga gaactcccta tcagtgatag agtttgtcct ccctatcagt
     1201 gatagatagg cttaaggagt aatacgactc actataggga gaaggaggcc attatggctc
     1261 tgtcgtcgaa aatcgtcggg atgcattacc ggtatcccga tttttatgaa gtcggccggg
     1321 agaagatccg cgagcatgca ctcgcgatca agaacgacga gacgtacttc tacgacgaag
     1381 acgcggccgc ggagctgggc catgacgccc tgcccgcgcc actcactttc atctgcatct
     1441 tcggctatca ggcgcagtcc gcgttcttcg atcacgccga catcggcgtg cgtgaggcca
     1501 agatcgtcca ggtcgaccag gaactgaagt tcttcaagcc gatcaaggcc ggtgaccgtt
     1561 tgtactgcga cgtctacgtc cacgcggttc gacgcgcgca cggcaccgac atcatcgtga
     1621 ccaagaacat aatcaccaac gacgctggtg agattgtcca ggaggcctac acgaccctcg
     1681 ccgggcgtgc gggcgacgga gaagagggtt tttctgatgg ctctgcgaag cttggtgcgg
     1741 ccgcgggcaa cacgcctggc atcaatctga tcaaggagga catgcgtgtc aaggtgcata
     1801 tggagggcaa cgtgaacggt cacgccttcg tgatcgaggg ggagggtaag ggtaagcctt
     1861 acgaagggac ccagaccgcc aacctcaccg tcaaggaggg cgcccctctc ccattctcgt
     1921 acgatatcct cactaccgcg gtgcattacg ggaaccgcgt gttcaccaag taccccgagg
     1981 atatccctga ctacttcaag cagagcttcc cggagggata ctcctgggag cggacgatga
     2041 ccttcgaaga caagggtatc tgtacgatcc gcagcgacat ctcgctggaa ggggactgtt
     2101 tcttccagaa cgtccgtttc aaggggacca actttccgcc gaacggcccg gtcatgcaga
     2161 agaagaccct caagtgggag ccgagcacgg agaagctgca cgttcgggac ggcctcctgg
     2221 tcggcaacat caacatggcg ctgctgctgg aaggcggcgg ccactaccta tgcgacttca
     2281 agaccacgta caaagcgaag aaggtcgtcc agctgcccga cgcgcacttc gtggaccacc
     2341 gcatcgaaat cctggggaac gattcggact acaacaaggt caagttgtac gagcacgctg
     2401 tggcacgcta tagcccgctc ccgtcgcagg tctggttcga aggcggcggc ggggagaacc
     2461 tgtatttcca ggactacaag gacgacgacg acaagtgatt taaataaccc cttggggcct
     2521 ctaaacgggt cttgaggggt tttttgttaa ctagcgtacg atcgaattcc tgcagctcac
     2581 ggtaactgat gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa
     2641 aatgccggcc tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt
     2701 atcaccaccg actatttgca acagtgccgt tgatcgtgct atgatcgact gatgtcatca
     2761 gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat cggtcagctt
     2821 ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc acagctcctt ccgtagcgtc
     2881 cggcccctcg aagatgggcc acttggactg atcgaggccc tgcgtgctac gctgggtccg
     2941 ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt cgatcctgcc
     3001 acgtcgcccg ttacaccgga ccttggagtt gtctctgaca cattctggcg cctgccaaat
     3061 gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg ggccacaggc agagcagatc
     3121 atctctgatc cattgcccct gccaccttac tcgcctgcaa gcccggtcgc ccgtgtccat
     3181 gaactcgatg ggcaggtact tctcctcggc gtgggacacg atgccaacac gacgctgcat
     3241 cttgccgagt tgatggcaaa ggttccctat ggggtgccga gacactgcac cattcttcag
     3301 gatggcaagt tggtacgcgt cgattatctc gagaatgacc actgctgtga gcgctttgcc
     3361 ttggcggaca ggtggctcaa ggagaagagc cttcagaagg aaggtccagt cggtcatgcc
     3421 tttgctcggt tgatccgctc ccgcgacatt gtggcgacag ccctgggtca actgggccga
     3481 gatccgttga tcttcctgca tccgccagag gcgggatgcg aagaatgcga tgccgctcgc
     3541 cagtcgattg gctgagctca tgagcggaga acgagatgac gttggagggg caaggtcgcg
     3601 ctgattgctg gggcaacacg ggggatccac tagttccatg agcgtcagac cccgtagaaa
     3661 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3721 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3781 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     3841 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3901 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3961 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4021 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4081 ccacgcttcc cgaggggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4141 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4201 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4261 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4321 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4381 gagctgatac cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc cgcggtaccc
     4441 ggggatcctc tagagtcgac caccaagggc accatctctg cttgggccac cccgttggcc
     4501 gcagccagct cgctgagagc cgtgaacgac agggcgaacg ccagcccgcc gacggcgagg
     4561 gttccgaccg ctgcaactcc cggtgcaacc ttgtcccggt ctattctctt cactgcacca
     4621 gctccaatct ggtgtgaatg cccctcgtct gttcgcgcag gcggggggct ctattcgttt
     4681 gtcagcatcg aaagtagcca gatcagggat gcgttgcaac cgcgtatgcc caggtcagaa
     4741 gagtcgcaca agagttgcag acccctggaa agaaaaatgg ccagagggcg aaaacaccct
     4801 ctgaccagcg gagcgggcga cgggaatcga acccgcgtag ctagtttgga agaatgggtg
     4861 tctgccgacc acatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc
     4921 taagtggaaa gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt
     4981 tgcaacccgg tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg
     5041 atcgctgaag actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg
     5101 tgtgaggtac tacgcgctgc agacctacga caacaagatg gacgccgaag cctggctcgc
     5161 gggcgagaag cggctcatcg agatggagac ctggacccct ccacaggacc gggcgaagaa
     5221 ggcagccgcc agcgccatca cgctggagga gtacacccgg aagtggctcg tggagcgcga
     5281 cctcgcagac ggcaccaggg atctgtacag cgggcacgcg gagcgccgca tctacccggt
     5341 gctaggtgaa gtgg
//
