LOCUS       sequence-345512-        8010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn 
            spacer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Altae-Tran H, Kannan S, Demircioglu FE, Oshiro R, Nety SP, McKay LJ,
            Dlakic M, Inskeep WP, Makarova KS, Macrae RK, Koonin EV, Zhang F
  TITLE     The widespread IS200/IS605 transposon family encodes diverse 
            programmable RNA-guided endonucleases.
  JOURNAL   Science. 2021 Oct;374(6563):57-65. doi: 10.1126/science.abj6856. 
            Epub 2021 Sep 9.
  PUBMED    34591643
REFERENCE   2  (bases 1 to 8010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abj6856"; journalName: "Science"; date: "2021-10"; 
            volume: "374"; issue: "6563"; pages: "57-65"
COMMENT     Sequence Label: pHS0537 CRISPR-associated IscB locus (Delaware...
FEATURES             Location/Qualifiers
     source          1..8010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(29..820,821..889))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     652..671
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(890..994)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1062..1080
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(1107..1112,1113..1129,1130..1135)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(5184..5203)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             6661..6852
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6846..6868)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6954..7094
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7009..7028
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7109..7126)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7280..7868)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7360..7379)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
       61 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
      121 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
      181 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
      241 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
      301 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
      361 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
      421 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
      481 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
      541 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
      601 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
      661 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
      721 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
      781 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
      841 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
      901 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
      961 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     1021 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     1081 cctttcgtct tcaagaattc tcatgtttga cagcttatca tcgataagct ttaatgcggt
     1141 agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg
     1201 ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg
     1261 gtactgccgg gcctcttgcg ggattagagg attcattaac ctaacctgca ccaagtcgaa
     1321 ggatgcagag ccgtttgcgc cgataggctt caaactggag ccggttgacg ttctcgacga
     1381 actagggcgg ccactagcag aagacgatgg aagccctgtc cagtcttgca ttcttgagca
     1441 atgcgaaatc gctgaaccaa ctaaggctca gacactaaca agcaagcagc aggaagcaag
     1501 agcgctatac cttgagctaa gaacgcaaag cgaaaatggc attgtgacca gagctgcttg
     1561 gtacaaaggg cttgaggtgg cgggtatcac gccccacgac aaaaccaaga aacaactcaa
     1621 ggacaagatg gtggaggcag gtgtctttct agttcgtgag gagggctcaa gggatgaata
     1681 ttttttgcga tctgaccaca tcagttttga agctacctaa cctggattga tgccttaccc
     1741 aaccttacct taccccctac ggggggaggt taggtaaggt taggttggca caatgggtca
     1801 tcacggttag gctggcgtta ggctggcgtt aggcaaaagt aaggtaaaag gttaggcaaa
     1861 agtaaggtcg attatgaaac agcaatccaa gctaagactg gcgaagtttt ttgcagatcc
     1921 gcttgagcaa tccaagctat tggaagagtt gcgacgtgga gcgccactag cagctgcatt
     1981 gtcaatgcga cggctgccga tcaatgcagg cgaacagttg ttgcgaatcc agcgtggtgg
     2041 cgatgtggac gccgctgtac gggctgggcg tctggaagca aggaaggccg atagtcgctt
     2101 gaaagattgg gcggcaaggc accggcaaga aatcgaggca gctgagaacg cggctaaccg
     2161 cgcctgaacc taattgatcg cttgcgcttg ttttgcccta ccggcccagg tggcgggtgt
     2221 tgcgaattca aacagtcttc ttaacgccgc gcacgtgaag gtctgatgct aaccgtagga
     2281 atcgaattgt caggattcaa gcgggataaa atcaaaaaca ggcatactag ccaacaatat
     2341 agccaacagt tgtttcgtaa tgaatgttgc cttttttgtt gcccttatgg gttgttatca
     2401 cttgtcaggc cagtaattga ctggtggtta gattcctaat gtagccatca agggcaacat
     2461 cgctatgagt tcgctaatag atcgagtgga actcatcact taccaccgac aacacaaaac
     2521 caatattagg ttttgtaaaa cataataacg ggttcaaaca aatgccaatc agcggattca
     2581 aggaagtcgg cgaagagcta gagcggttgc gccaggagat tgacgaacta cgtctagacc
     2641 tgcaagtggt tgcaaaggcg gcaggtgtgg aagcgcagct cgatgctgca cgttctgaac
     2701 gtcaactgca caaggcagag gcacaggcag caatcaagcg cctggacgag cgattgggct
     2761 ctttgtagcg ctttcgttca ggattcgctg ataacggtct ggcgtggctc tggagccttc
     2821 agaaagccat agccatagcc gtcggaattg aagagcttgg tcatatgctt tgtgtggcat
     2881 tgtatgcgtt taccagtctt ggacttgaac ccagctaaag gcttcgatgt tgtagtttcg
     2941 gcaatacgac tgacgtaggt gccgacatac ttgcccttgg gaatttcagc ccgaacaatg
     3001 tcgccggtgc gaaacggatg aaccggaatt cctttggcct tacctctagg gaaaccgtaa
     3061 gcatctacgt tgacaacctg ccgtttcccg tgccccatcg ccttgatcgc tagtggtctg
     3121 accgcttccc actgcagcac ttccggtgta ctggctccga cacaagcggc atccagccag
     3181 tgtgtcttgg gaatctgtcg ctcagaacga ttgaacttgg tgcggccacc gctggcaact
     3241 tccacaggca agccagtagc tttcagacgt tccagtagag cgtatcgggt tgagttgacg
     3301 gctgcagcat ccttcagtgg agccttggcc tgacgttgga gtttctgcaa cacttccggt
     3361 ttttttgcca ggaacacggc tgcaggacgt tggttctttc ttttgttgca gggttcacaa
     3421 gatagcgtca ggttgctgac ccgatcactg ccgccgtggc tacgtggaac aatgtgatcc
     3481 acttccaaac gcttgtcctt ggcaccgcaa tagccgcact gtcgccgcca cttgtccaac
     3541 aggtattccc gcacctcgta accgaataga gtgccctgct ggtactccac gccactgatt
     3601 tccgggttct gcatcttctg catatcaaag cgcacaatct cttgtgaaat cgccgtgatg
     3661 ggcaggtagc gccgcaagcg atgcacccag gtcatgatga cttcaatgcg atgttggagc
     3721 gatggagcga gccacttccc caacgtagaa accgtgtcag cactggatag caaaggtggg
     3781 ccagtacgct tgcgattcag ccaacgggca ggacggtagc gggtcttgcg gttgcgtctt
     3841 ccacggcgta gcgatcggcg ggattccagg gcattcttga tcagctgacc acggtgctgg
     3901 agttctgccg cccagaccac ttcgccagtg gcttctgtga ctacggcaat gccggtagtc
     3961 ttgctgcctg gatctagttt cagccgcaca ggtcgtgggc ttgcggtagg gtaggattgt
     4021 ttgaggatga tagtgaatgg gtatcgacgc aacaccgctg ccttgccact gttcagtagc
     4081 aggcgagccc gtcttggcgt acaaggcgcc agcggacaac gatcagcgtc aacaaccaag
     4141 actctggaca ttgaattagc cttaaagcta gcgatcgggc gcaacttccg aagaagcgtt
     4201 gccgtcacgt aaagtatgcc tcgacaatgt tatcgccgct tttgacgccg tacacactgg
     4261 gcaccctcca aagtccctgt ttaatgcacg accatagagc cacaggctgg cacgcacccg
     4321 tgggtatgat gacgacaata acgtagttcc caaagaactt agtctggttg tagctgcgga
     4381 aaaaatccgc actcatttcc ggaaaactgt caaccaccgc ccattttttt gaacgccttt
     4441 tgccttccag cccagcatcc aagcagggta gcgcgggcac tccctgacag caccccgaaa
     4501 atgagtggag gctacaacga gaagtcattt aataaggcca ctgttaaacc tgacagcacc
     4561 ccgaaaatga gtggaggcta caactgggag gagtggtttg agttccaact ttttcctgac
     4621 aaccccccga aaatgagtat agcgccacca ccttcaatcg ggccgaatat aacgccagtg
     4681 ggtgcagatg taaacaatcg gcttctggag cccatcacaa gcaacacctt tccttttccc
     4741 gttcgtaccg tgcctgccgc ttatacttcc gctccatctc ttccttcgtt ggcatcaccg
     4801 gatcgggaac atccaagagc cagcggagct ccgcaaaagc cacaatgtcc accggatgat
     4861 tcttggtact caggaagcgc cgccagcgat tacgttcgcg ttccggccag gtcatcattt
     4921 gcaggctact gtcatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
     4981 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
     5041 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
     5101 ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg
     5161 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     5221 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     5281 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     5341 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     5401 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     5461 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     5521 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     5581 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     5641 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     5701 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     5761 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     5821 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
     5881 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     5941 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     6001 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     6061 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg
     6121 cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggcagc
     6181 gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc
     6241 tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc
     6301 gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt
     6361 gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt ccggatctgc
     6421 atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac gaagcgctgg
     6481 cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag ttgtttaccc
     6541 tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc
     6601 tctcgtttca tcggtatcat tacccccatg aacagaaatc ccccttacac ggaggcatca
     6661 gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta
     6721 acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat ctgtgaatcg
     6781 cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt
     6841 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     6901 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     6961 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     7021 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     7081 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7681 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     7741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7921 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7981 attaaaaatg aagttttaaa tcaatctaaa
//
