LOCUS       sequence-345522-        5795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1352:Dendra
FEATURES             Location/Qualifiers
     source          1..5795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1970..2558)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2050..2069)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2683..3483)
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             complement(3740..3763)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(5437..5456)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5438..5456)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cggtgctggc gctcgcggcc aggcgtggtt cttcgcgtac tgacaagaaa cccccctctt
       61 taggactcag tgtccttggg aggggggctt tttgcgtttc aggaggtctt ggccagcttg
      121 gacatcgcct cagcgatagc ctcgtcgcgg gcctcagacg ccatctggta cttcatcgcc
      181 atcctaggag tcgtgtgacc gagacgggcc atcagctcct tggtcgtcgc acctgcctga
      241 gcggcgaacg tagcgccgac agcgcggagg tcgtggatgc ggagttccgg ccgaccgatc
      301 ttggcgtagc cacgcttcag cgacttggtg aacgcggact tcgacagccg gttgccctgc
      361 gtcgtggtca ccaggaatgc ctcggggccc ttgttcatct tcgtacggtc cttcatgtgc
      421 gctcggatca tctccgcgac gtgaggcgga accgtcacag gacgcttcga ccggacggtc
      481 ttggcgttgc caacgacgat cttgttcccc acgcgggaag cgccacggcg cacccggagc
      541 ttcatcgtca tgccgtcgtc cacgatgtcc ttgcggcgaa gctcgatcag ctctccgaac
      601 cggaggctcg tccacgccag gatgtatgcc gcgatccggt agtgctcgaa gatctcagcg
      661 gcgacgatgt ccagctcctc aggcgtcagc gcctctacgt cgcgctcatc ggctgccttc
      721 tgctcgatcc ggcacgggtt ctctgcgatc agcttgtcct cgaccgctgt gttcatcacc
      781 gcccggagga cgttgtaggc atgccggcgg gcagtcgggt gcttcctacc catcccggcc
      841 caccacgcac gcaccagagc tggcgtcatc tctgtgaccg ccacttcacc tagcaccggg
      901 tagatgcggc gctccgcgtg cccgctgtac agatccctgg tgccgtctgc gaggtcgcgc
      961 tccacgagcc acttccgggt gtactcctcc agcgtgatgg cgctggcggc tgccttcttc
     1021 gcccggtcct gtggaggggt ccaggtctcc atctcgatga gccgcttctc gcccgcgagc
     1081 caggcttcgg cgtccatctt gttgtcgtag gtctgcagcg cgtagtacct cacaccgtcc
     1141 tgcgggttga cgtatgaggc ttggatcctc ccgctgcgct gagtcttcag cgatccccat
     1201 ccgcgacgtg ccaactaggt ctcctctcgt cgtgaacaag gctaccgggt tgcaactcct
     1261 gtgcaactct caggcttcaa cgcgcttcta cgacctgcaa tttctttcca cttagaggat
     1321 gcagccgaga gggggtaaaa acctatcttg accggcccat atgtggtcgg cagacaccca
     1381 ttcttccaaa ctagctacgc gggttcgatt cccgtcgccc gctccgctgg tcagagggtg
     1441 ttttcgccct ctggccattt ttctttccag gggtctgcaa ctcttgtgcg actcttctga
     1501 cctgggcata cgcggttgca acgcatccct gatctggcta ctttcgatgc tgacaaacga
     1561 atagagcccc ccgcctgcgc gaacagacga ggggcattca caccagattg gagctggtgc
     1621 agtgaagaga atagaccggg acaaggttgc accgggagtt gcagcggtcg gaaccctcgc
     1681 cgtcggcggg ctggcgttcg ccctgtcgtt cacggctctc agcgagctgg ctgcggccaa
     1741 cggggtggcc caagcagaga tggtgccctt ggtggtcgac tctagaggat ccccgggtac
     1801 cgcggccgca cgcgttgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1861 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1921 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1981 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2041 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2101 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ctcgggaagc gtggcgcttt
     2161 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2221 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2281 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2341 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2401 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2461 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2521 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2581 cggggtctga cgctcatgga actagtggat cccccgtgtt gccccagcaa tcagcgcgac
     2641 cttgcccctc caacgtcatc tcgttctccg ctcatgagct cagccaatcg actggcgagc
     2701 ggcatcgcat tcttcgcatc ccgcctctgg cggatgcagg aagatcaacg gatctcggcc
     2761 cagttgaccc agggctgtcg ccacaatgtc gcgggagcgg atcaaccgag caaaggcatg
     2821 accgactgga ccttccttct gaaggctctt ctccttgagc cacctgtccg ccaaggcaaa
     2881 gcgctcacag cagtggtcat tctcgagata atcgacgcgt accaacttgc catcctgaag
     2941 aatggtgcag tgtctcggca ccccataggg aacctttgcc atcaactcgg caagatgcag
     3001 cgtcgtgttg gcatcgtgtc ccacgccgag gagaagtacc tgcccatcga gttcatggac
     3061 acgggcgacc gggcttgcag gcgagtaagg tggcaggggc aatggatcag agatgatctg
     3121 ctctgcctgt ggccccgctg ccgcaaaggc aaatggatgg gcgctgcgct ttacatttgg
     3181 caggcgccag aatgtgtcag agacaactcc aaggtccggt gtaacgggcg acgtggcagg
     3241 atcgaacggc tcgtcgtcca gacctgacca cgagggcatg acgagcgtcc ctcccggacc
     3301 cagcgtagca cgcagggcct cgatcagtcc aagtggccca tcttcgaggg gccggacgct
     3361 acggaaggag ctgtggacca gcagcacacc gccgggggta accccaaggt tgagaagctg
     3421 accgatgagc tcggcttttc gccattcgta ttgcacgaca ttgcactcca ccgctgatga
     3481 catcagtcga tcatagcacg atcaacggca ctgttgcaaa tagtcggtgg tgataaactt
     3541 atcatcccct tttgctgatg gagctgcaca tgaacccatt caaaggccgg cattttcagc
     3601 gtgacatcat tctgtgggcc gtacgctggt actgcaaata cggcatcagt taccgtgagc
     3661 tgcaggaatt cgatcgtacg ctagttaaca aaaaacccct caagacccgt ttagaggccc
     3721 caaggggtta tttaaatcac ttgtcgtcgt cgtccttgta gtcctggaaa tacaggttct
     3781 ccccgccgcc gccttcgaac cagacctgcg acgggagcgg gctatagcgt gccacagcgt
     3841 gctcgtacaa cttgaccttg ttgtagtccg aatcgttccc caggatttcg atgcggtggt
     3901 ccacgaagtg cgcgtcgggc agctggacga ccttcttcgc tttgtacgtg gtcttgaagt
     3961 cgcataggta gtggccgccg ccttccagca gcagcgccat gttgatgttg ccgaccagga
     4021 ggccgtcccg aacgtgcagc ttctccgtgc tcggctccca cttgagggtc ttcttctgca
     4081 tgaccgggcc gttcggcgga aagttggtcc ccttgaaacg gacgttctgg aagaaacagt
     4141 ccccttccag cgagatgtcg ctgcggatcg tacagatacc cttgtcttcg aaggtcatcg
     4201 tccgctccca ggagtatccc tccgggaagc tctgcttgaa gtagtcaggg atatcctcgg
     4261 ggtacttggt gaacacgcgg ttcccgtaat gcaccgcggt agtgaggata tcgtacgaga
     4321 atgggagagg ggcgccctcc ttgacggtga ggttggcggt ctgggtccct tcgtaaggct
     4381 tacccttacc ctccccctcg atcacgaagg cgtgaccgtt cacgttgccc tccatatgca
     4441 ccttgacacg catgtcctcc ttgatcagat tgatgccagg cgtgttgccc gcggccgcac
     4501 caagcttacc caccacggca aagtttgtct tgagctcgcc gatgacctcg tcccacaccg
     4561 gctcgggaag ttcgtgaccc atcccgtcga tgagcaccat gcgcgcaccc cggatcgcat
     4621 tggcgatcgc gcgcccgccc gacggaggca tcaaccggtc ggcgcgtccg tggatcacca
     4681 cggtgggcgc ggtgacgcgt tcgttgtgat gacgcaggct gccgctgccc aggatcgcgg
     4741 cgaaatggcg ggcgatgcct gcggggtagt acgcgcggtc gtacagttcg gcggcctcgg
     4801 cccggatctc ctcttcggag cgcgggtacg ccgggctgcc gttgagcctg ctgagtcgaa
     4861 cggcgttgtc cagcacggca tctcgcgacg cgtcggcagg cggtccggtg atcagcgaca
     4921 gcagctgtct gattccgggc ggcgggagga gcgcctggtt gttgctggag aagatcaccc
     4981 cgagggtctc ggtgcgggcc gcgtgctgcg acgcgaacac ctgggcgatc atgccgccca
     5041 tggacccacc gacgatgtgc gcgcggtcga tctccaagtg gtcgagcagg gccgcagcgt
     5101 cgtcggccat gtcctcgagc gtgtacaccg cggggctgcg cttaccgagg aacgagcgcg
     5161 ccatccgcag ggcgagcggc gaatcgacac gctgccccga cagtttcgtc gacaggccga
     5221 cgtcgcggtt gtcgaaccgg atgacgcgca acccctgatc gacgagtttc tcgcagaact
     5281 cggtacgcca catcagcatc tgcgcaccca gacccatgat caacaggacc gcaggatcgc
     5341 cgggatcacc catgtcctcg tagaagaggt cgatcgggtg gtcggcgtcg ccggcgcgcg
     5401 cgagcccggt gcgggtctgc ataatggcct ccttctccct atagtgagtc gtattactcc
     5461 ttaagcctat ctatcactga tagggaggac aaactctatc actgataggg agttctcccg
     5521 ctcgtcagag acccttctca caccgggcct gtgcgtgcga acgcacagat caacgccggg
     5581 atggcgtaga gctcactgcg cagcaccgtg gggatgcggc cgatcatcac gtcgcggatc
     5641 gtgccgcctc ccacggccgt gacggtgccg acgatccaat atttcgacgt ccccgatcaa
     5701 agcgggcgcg ttgtggtcac cgtctagatt tctggcggga acggcaatgc tcctggcggc
     5761 ggcggggctg gtgcccaggt ctccgcacag agcgg
//
