LOCUS       sequence-345525-       10608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsHelz.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Hanet A, Rasch F, Weber R, Ruscica V, Fauser M, Raisch T, 
            Kuzuoglu-Ozturk D, Chang CT, Bhandari D, Igreja C, Wohlbold L
  TITLE     HELZ directly interacts with CCR4-NOT and causes decay of bound 
            mRNAs.
  JOURNAL   Life Sci Alliance. 2019 Sep 30;2(5). pii: 2/5/e201900405. doi: 
            10.26508/lsa.201900405. Print 2019 Oct.
  PUBMED    31570513
REFERENCE   2  (bases 1 to 10608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Life Sci 
            Alliance."; date: "2019-09-30"; volume: "2(5). pii: 2/5/e201900405. 
            doi"; pages: " 10.26508/lsa.201900405. Print 2019 Oct"
COMMENT     Sequence Label: pT7-EGFP-C1-HsHelz_AG
FEATURES             Location/Qualifiers
     source          1..10608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     553..572
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        553..571
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      599..608
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(605..607,608..610,611..1321)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(605..607,608..610,611..1321)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(650..671)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(911..930)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1258..1279
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7336..7457
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7373..7392)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7427..7446
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7464..7919)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7601..7622)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7813..7832
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7946..8050
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8052..8409
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8052..8243
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8075..8095)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8260..8395
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8444..9238
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8444..9238
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8498..8517)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9108..9127
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9426..9445)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9470..9517
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9846..10434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10335..10354
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      541 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      601 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      661 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      721 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      781 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      841 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      901 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      961 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1021 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1081 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1141 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1201 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1261 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1321 gtccggactc agatctcgag ctcaagcttc gaattctgca gtcgacatgg aagacagaag
     1381 agctgaaaag tcatgtgaac aagcatgtga atcacttaag aggcaggact atgaaatggc
     1441 cctcaagcac tgcacagagg cccttctttc tcttggccag tactccatgg cagacttcac
     1501 agggccttgt ccattggaaa tagaacgcat caaaatcgag agtcttctct acagaattgc
     1561 ctcatttttg caactgaaaa attatgtgca agctgatgaa gattgtagac atgtgctggg
     1621 agaaggactg gccaagggag aagatgcctt tcgggcagtg ctttgctgca tgcagctgaa
     1681 agggaagctc caacctgtat ccaccattct tgccaagtca ctcacaggag agtccctgaa
     1741 tgggatggta acaaaggatt tgacaagact aaaaacactt ctctcagaaa cagagacagc
     1801 aactagtaac gccctctctg gatatcacgt ggaagactta gatgaggggt cttgtaatgg
     1861 ttggcatttc cgcccaccac ctaggggaat cacaagcagc gaggaatata ctttgtgtaa
     1921 aagattttta gaacaaggaa tctgtaggta tggtgcccag tgtacttcag cacattccca
     1981 ggaagaacta gcagaatggc agaaaagata tgcttcacgg ctgataaaat tgaaacagca
     2041 aaatgagaat aaacagctct caggcagtta catggaaacc ttgatagaaa agtggatgaa
     2101 ttcattgtct cctgagaaag tgcttagtga atgtatagaa ggagtaaagg tagagcacaa
     2161 tcctgacctg tcagttactg tcagcaccaa aaaatcccac cagacatgga cctttgctct
     2221 cacttgtaag cctgcaagaa tgctgtatcg tgtagcattg ctttatgatg ctcatcgtcc
     2281 tcattttagt atcattgcaa tatctgccgg agatagtact acccaggtat cacaagaagt
     2341 cccagaaaac tgtcaagaat ggataggagg aaagatggcc caaaatggat tagatcatta
     2401 cgtgtataaa gtcgggatag catttaacac agaaatattt ggaacttttc gccaaaccat
     2461 agttttcgac tttggattgg aaccagtact catgcaaaga gtaatgattg atgcagcttc
     2521 tacagaagat ctcgaatacc tgatgcatgc aaaacagcag ctagtaacca cagctaaacg
     2581 ttgggattct tcctctaaga ctattataga ttttgaacct aatgaaacta ctgatttgga
     2641 gaagagcctt cttatcagat accaaattcc cctctctgct gaccagctat ttactcagtc
     2701 cgttttagac aaatcattga ccaagagcaa ctatcagtca cggttacatg accttcttta
     2761 tattgaggag atagcccagt ataaagaaat cagcaagttc aaccttaaag tgcaattgca
     2821 gattctggca agcttcatgc tcactggtgt ttctggaggt gcaaagtatg ctcagaatgg
     2881 acaacttttt ggtcgcttta agcttactga aacactttct gaagatactt tggctggacg
     2941 actggtgatg accaaagtca atgctgttta tttattacca gtccctaaac agaagttagt
     3001 acagacccag ggaaccaaag agaaggttta tgaagctact attgaagaaa aaacaaagga
     3061 atatatattt ttaaggctat ctagggaatg ctgtgaagaa cttaatcttc ggcctgactg
     3121 tgacacacag gttgaacttc agtttcaatt aaatcgatta cccctctgtg aaatgcacta
     3181 tgcactagac aggatcaagg acaatggggt tttgtttcca gacatcagta tgactcccac
     3241 cataccatgg agtcctaaca gacaatggga tgaacagttg gatcctcgac taaatgcaaa
     3301 acagaaagag gctgttctgg ccattaccac tccacttgca atccagctgc cgcctgtgct
     3361 tatcatcgga ccctatggga caggcaaaac gttcactcta gctcaggctg tcaaacatat
     3421 tctgcagcaa caggagacta ggattctcat ttgcacccat tctaatagtg ctgctgatct
     3481 ctacataaag gattatttac atccatatgt agaagcaggc aatccccagg caagacctct
     3541 cagggtatat ttcagaaatc gctgggtaaa gactgtccac ccagttgtgc atcagtactg
     3601 tttgatctca agcgcacatt ccacctttca gatgccccag aaagaagata ttcttaaaca
     3661 tcgagtggtg gttgttacct tgaatacttc ccagtacctc tgtcagttgg accttgaacc
     3721 tgggtttttt acacacattc tattagatga agctgcccag gccatggagt gtgaaaccat
     3781 tatgcctcta gcattagcaa ctcaaaacac tcggattgtc ttggctggtg atcacatgca
     3841 gctcagtcct tttgtttaca gcgagtttgc cagggagaga aaccttcacg tttcattact
     3901 tgaccgactc tatgagcatt accctgctga gttcccatgt aggattctcc tgtgtgagaa
     3961 ctaccgctcc catgaagcta tcatcaatta tacctctgag cttttctatg agggcaaact
     4021 gatggccagt gggaagcagc cagcacacaa agatttctac ccactaactt tctttacagc
     4081 acgaggagaa gatgtacaag aaaaaaatag cacagctttt tataataatg cagaggtgtt
     4141 tgaagtggtg gaacgtgtag aagagttaag aaggaagtgg ccagtagcgt gggggaagtt
     4201 agatgatggc agtattggtg tggtgactcc atatgctgat caagtgttta gaatacgtgc
     4261 tgaacttcga aaaaagagat tatctgatgt taatgtagaa agggtgctaa atgttcaagg
     4321 aaagcaattc agagttttgt ttcttagcac agtacgtaca agacatactt gtaaacataa
     4381 acagacacca attaaaaaga aagagcaact tctggaagat tccacagagg acttagatta
     4441 tggtttttta tctaactaca agcttctcaa tactgccatc acaagagcac aatccctggt
     4501 tgctgtggtg ggtgatccca ttgctctgtg ctctattgga agatgcagga aattttggga
     4561 acggtttatt gccctgtgtc atgaaaacag tagcctacat ggaatcactt ttgaacagat
     4621 caaagcccag ttagaggctt tagaactaaa gaagacatat gtgttgaatc cgctggcacc
     4681 tgaatttatc ccccgggctc taagactgca gcattcagga agtaccaaca aacagcagca
     4741 atcaccaccc aaggggaaaa gtcttcatca tacccagaat gatcacttcc agaatgatgg
     4801 aattgttcag cccaatcctt ctgtacttat tggcaatcct attagagcat atactcctcc
     4861 accccctctt ggacctcacc caaatttggg aaaatctcca agccctgttc aaagaataga
     4921 tcctcacact gggacaagta ttctttatgt acctgctgtc tatggaggga atgtagttat
     4981 gtcggtgcct ttacctgtac catggacagg ataccagggt aggtttgcag ttgatcctcg
     5041 aattattaca catcaggcag caatggccta taacatgaac ctattacaga cacatggacg
     5101 aggatctcct attccttatg gccttggaca tcacccacct gtcaccatag gccagccaca
     5161 aaatcagcat caggagaagg atcaacatga gcaaaatcga aatggtaaaa gtgatacaaa
     5221 taattccgga cctgaaatta ataagattcg aacaccagag aaaaagccaa cagaaccaaa
     5281 acaggttgat ttggaatcaa atccacagaa cagaagtcct gaatcacgtc ctagtgttgt
     5341 ttatcccagt accaaatttc ctcgcaaaga taatctcaac ccaagacaca taaatcttcc
     5401 ccttcctgct ccccacgcac agtatgcaat ccctaatcgc cactttcatc cccttcccca
     5461 gctaccaaga ccaccctttc caattccaca gcagcacacc ttgttaaatc agcagcagaa
     5521 taatttgcct gaacaaccaa atcagatacc acctcagcca aatcaggtag tccagcagca
     5581 aagtcagttg aatcagcagc ctcagcagcc acctcctcag ctttctcctg catatcaggc
     5641 gggacccaac aatgcttttt ttaatagtgc agttgctcat cggccacagt ctcctcctgc
     5701 agaagctgta attccggagc agcagccccc tcccatgctg caagaaggcc acagtcctct
     5761 gagagccatt gcacaacccg gccccattct tccttcacat ctgaatagct tcattgatga
     5821 gaacccctcg ggattaccta taggggaggc tttagatcgt atacatggga gtgtcgctct
     5881 ggaaacatta aggcagcagc aggcacggtt ccagcagtgg agcgagcatc atgcctttct
     5941 cagtcagggc agcgttccat acccacacca tcaccatcct cacctccagc atcttcctca
     6001 gccgcccctg ggattacatc agccgccagt gagggcagac tggaagctca ccagcagtgc
     6061 cgaagatgaa gtggagacca catactcaag gtttcaagac ttaatcagag aactgtctca
     6121 tcgtgatcaa agtgaaacac gggaactagc tgaaatgcca ccacctcaat caagactttt
     6181 gcaatataga caagtacaga gtagaagccc accagcagtc ccatctcccc catccagtac
     6241 agaccacagt agccactttt ctaactttaa tgataacagc agagacattg aagtagccag
     6301 caacccagca tttccacagc gcctcccacc ccagatattc aactcacctt tctcgttgcc
     6361 atctgaacac cttgcccctc ctcccttgaa atacctggca cctgatggag catggacttt
     6421 tgctaacttg caacagaatc acctaatggg gccaggtttt ccctatggcc tacctccatt
     6481 gcctcacagg ccaccgcaga acccttttgt acaaatacag aatcatcaac atgctattgg
     6541 tcaagagcca tttcacccat tgtcatctcg aacagtatct tcttcttcgc tccctagctt
     6601 agaagagtat gagcccagag gacctggtcg gcccttgtac caaagaagaa tctcatctag
     6661 ctcagttcaa ccttgttctg aagaagtaag cactcctcaa gacagtctgg ctcagtgtaa
     6721 agagcttcag gaccacagta accaatcttc tttcaacttt tcatccccgg agtcctgggt
     6781 aaacaccacc tcatctactc cttatcagaa cattccgtgc aatggatcca gcaggacagc
     6841 tcagcccaga gagttgatag cgccacccaa gactgtcaaa ccccctgagg atcaactgaa
     6901 gtcggagaac ctcgaggtgt ccagttcctt caactacagt gtgctgcagc atcttggcca
     6961 gtttccaccc cttatgccta acaagcagat cgcggagtcg gccaatagca gtagccccca
     7021 gagctctgcg gggggcaagc ccgccatgtc ctatgccagc gctctgcggg cccctccaaa
     7081 gcccaggccc cctcctgagc aggccaagaa gagtagcgac cctctgtctc tcttccagga
     7141 actgagccta gggagctcat ctggcagcaa tggcttttac tcatatttta aataaccgcg
     7201 ggcccgggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     7261 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     7321 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     7381 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     7441 ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     7501 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     7561 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     7621 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     7681 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     7741 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     7801 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     7861 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     7921 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7981 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8041 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     8101 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8161 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     8221 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     8281 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8341 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8401 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     8461 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     8521 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     8581 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     8641 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     8701 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     8761 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     8821 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     8881 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     8941 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     9001 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     9061 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     9121 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     9181 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     9241 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     9301 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     9361 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     9421 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     9481 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     9541 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     9601 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     9661 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     9721 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9781 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9841 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9901 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9961 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    10021 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    10081 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    10141 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    10201 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    10261 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    10321 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    10381 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    10441 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    10501 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata
    10561 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccg
//
