LOCUS       sequence-345567-        2922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conditionally active form of Cre recombinase (activated in response 
            to tamoxifen) under Syn promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Linghu C, An B, Shpokayte M, Celiker OT, Shmoel N, Zhang R, Zhang C,
            Park D, Park WM, Ramirez S, Boyden ES
  TITLE     Recording of cellular physiological histories along optically 
            readable self-assembling protein chains.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01586-7.
  PUBMED    36593405
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01586-7."
COMMENT     Sequence Label: pAAV-Syn-ERT2-iCre-ERT2
FEATURES             Location/Qualifiers
     source          1..2922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..939
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             1984..2919
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
ORIGIN
        1 atggctggag acatgagagc tgccaacctt tggccaagcc cgctcatgat caaacgctct
       61 aagaagaaca gcctggcctt gtccctgacg gccgaccaga tggtcagtgc cttgttggat
      121 gctgagcccc ccatactcta ttccgagtat gatcctacca gacccttcag tgaagcttcg
      181 atgatgggct tactgaccaa cctggcagac agggagctgg ttcacatgat caactgggcg
      241 aagagggtgc caggctttgt ggatttgacc ctccatgatc aggtccacct tctagaatgt
      301 gcctggctag agatcctgat gattggtctc gtctggcgct ccatggagca cccagtgaag
      361 ctactgtttg ctcctaactt gctcttggac aggaaccagg gaaaatgtgt agagggcatg
      421 gtggagatct tcgacatgct gctggctaca tcatctcggt tccgcatgat gaatctgcag
      481 ggagaggagt ttgtgtgcct caaatctatt attttgctta attctggagt gtacacattt
      541 ctgtccagca ccctgaagtc tctggaagag aaggaccata tccaccgagt cctggacaag
      601 atcacagaca ctttgatcca cctgatggcc aaggcaggcc tgaccctgca gcagcagcac
      661 cagcggctgg cccagctcct cctcatcctc tcccacatca ggcacatgag taacaaaggc
      721 atggagcatc tgtacagcat gaagtgcaag aacgtggtgc ccctctatga cctgctgctg
      781 gaggcggcgg acgcccaccg cctacatgcg cccactagcc gtggaggggc atccgtggag
      841 gagacggacc aaagccactt ggccactgcg ggctctactt catcgcattc cttgcaaaag
      901 tattacatca cgggggaggc agagggtttc cctgccacag ctgtcgacaa cctgctgact
      961 gtgcaccaaa acctgcctgc cctccctgtg gatgccacct ctgatgaagt caggaagaac
     1021 ctgatggaca tgttcaggga caggcaggcc ttctctgaac acacctggaa gatgctcctg
     1081 tctgtgtgca gatcctgggc tgcctggtgc aagctgaaca acaggaaatg gttccctgct
     1141 gaacctgagg atgtgaggga ctacctcctg tacctgcaag ccagaggcct ggctgtgaag
     1201 accatccaac agcacctggg ccagctcaac atgctgcaca ggagatctgg cctgcctcgc
     1261 ccttctgact ccaatgctgt gtccctggtg atgaggagaa tcagaaagga gaatgtggat
     1321 gctggggaga gagccaagca ggccctggcc tttgaacgca ctgactttga ccaagtcaga
     1381 tccctgatgg agaactctga cagatgccag gacatcagga acctggcctt cctgggcatt
     1441 gcctacaaca ccctgctgcg cattgccgaa attgccagaa tcagagtgaa ggacatctcc
     1501 cgcaccgatg gtgggagaat gctgatccac attggcagga ccaagaccct ggtgtccaca
     1561 gctggtgtgg agaaggccct gtccctgggg gttaccaagc tggtggagag atggatctct
     1621 gtgtctggtg tggctgatga ccccaacaac tacctgttct gccgggtcag aaagaatggt
     1681 gtggctgccc cttctgccac ctcccaactg tccacccggg ccctggaagg gatctttgag
     1741 gccacccacc gcctgatcta tggtgccaag gatgactctg ggcagagata cctggcctgg
     1801 tctggccact ctgccagagt gggtgctgcc agggacatgg ccagggctgg tgtgtccatc
     1861 cctgaaatca tgcaggctgg tggctggacc aatgtgaaca ttgtgatgaa ctacatcaga
     1921 aacctggact ctgagactgg ggccatggtg aggctgctcg aggatgggga cctcgagcca
     1981 tctgctggag acatgagagc tgccaacctt tggccaagcc cgctcatgat caaacgctct
     2041 aagaagaaca gcctggcctt gtccctgacg gccgaccaga tggtcagtgc cttgttggat
     2101 gctgagcccc ccatactcta ttccgagtat gatcctacca gacccttcag tgaagcttcg
     2161 atgatgggct tactgaccaa cctggcagac agggagctgg ttcacatgat caactgggcg
     2221 aagagggtgc caggctttgt ggatttgacc ctccatgatc aggtccacct tctagaatgt
     2281 gcctggctag agatcctgat gattggtctc gtctggcgct ccatggagca cccagtgaag
     2341 ctactgtttg ctcctaactt gctcttggac aggaaccagg gaaaatgtgt agagggcatg
     2401 gtggagatct tcgacatgct gctggctaca tcatctcggt tccgcatgat gaatctgcag
     2461 ggagaggagt ttgtgtgcct caaatctatt attttgctta attctggagt gtacacattt
     2521 ctgtccagca ccctgaagtc tctggaagag aaggaccata tccaccgagt cctggacaag
     2581 atcacagaca ctttgatcca cctgatggcc aaggcaggcc tgaccctgca gcagcagcac
     2641 cagcggctgg cccagctcct cctcatcctc tcccacatca ggcacatgag taacaaaggc
     2701 atggagcatc tgtacagcat gaagtgcaag aacgtggtgc ccctctatga cctgctgctg
     2761 gaggcggcgg acgcccaccg cctacatgcg cccactagcc gtggaggggc atccgtggag
     2821 gagacggacc aaagccactt ggccactgcg ggctctactt catcgcattc cttgcaaaag
     2881 tattacatca cgggggaggc agagggtttc cctgccacag ct
//
