LOCUS       sequence-345586-        5598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0863:Dendra
FEATURES             Location/Qualifiers
     source          1..5598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1400..1423
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1680..2480
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      2605..3193
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3094..3113
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5502..5521
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5502..5520
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actcaagggc gcctacccgc acatcgatct gctgatcaac aacgccggcg tgatgtatcc
       61 gccaaagcag accaccgtcg acgggttcga gctgcagttc ggcaccaacc atctggggcc
      121 gttcgcgctg acgggtctgc tcatcgacca cctgctgccg gtcgagggct cacgcgtggt
      181 ggccgtcgcc agcgtggccc accgcatccg cgccaagatc cacttcgagg atctgcagtg
      241 ggaacgccgc tacaaccggg tcgaggcgta cggccagtcc aagctcgcga atctgttgtt
      301 cgcctacgaa cttcagcgtc gcctcgccgc ggcaggcaag ccgaccatct cggtggccgc
      361 ccatcccggg ttgtcgaaca ccgaactcat gcggcacatc cccggcaccg ggctgcccgg
      421 ctaccaccag atcgcgagcc tgttctccaa cagtccgctg atgggcgcgc tggcgacgct
      481 gcgcgcggcc accgatcccg gtgtcaaggg cggccagtac tacggcccgg acgggttccg
      541 tgaggtgcgt ggacaccccg agctcgtgaa atccagttcc cagtcccgtg atccggagtt
      601 gcaacgccgg ttgtgggccg tctcagaaga gctgaccggc gtcagctacc cggtgaagct
      661 tggtgcggcc gcgggcaaca cgcctggcat caatctgatc aaggaggaca tgcgtgtcaa
      721 ggtgcatatg gagggcaacg tgaacggtca cgccttcgtg atcgaggggg agggtaaggg
      781 taagccttac gaagggaccc agaccgccaa cctcaccgtc aaggagggcg cccctctccc
      841 attctcgtac gatatcctca ctaccgcggt gcattacggg aaccgcgtgt tcaccaagta
      901 ccccgaggat atccctgact acttcaagca gagcttcccg gagggatact cctgggagcg
      961 gacgatgacc ttcgaagaca agggtatctg tacgatccgc agcgacatct cgctggaagg
     1021 ggactgtttc ttccagaacg tccgtttcaa ggggaccaac tttccgccga acggcccggt
     1081 catgcagaag aagaccctca agtgggagcc gagcacggag aagctgcacg ttcgggacgg
     1141 cctcctggtc ggcaacatca acatggcgct gctgctggaa ggcggcggcc actacctatg
     1201 cgacttcaag accacgtaca aagcgaagaa ggtcgtccag ctgcccgacg cgcacttcgt
     1261 ggaccaccgc atcgaaatcc tggggaacga ttcggactac aacaaggtca agttgtacga
     1321 gcacgctgtg gcacgctata gcccgctccc gtcgcaggtc tggttcgaag gcggcggcgg
     1381 ggagaacctg tatttccagg actacaagga cgacgacgac aagtgattta aataacccct
     1441 tggggcctct aaacgggtct tgaggggttt tttgttaact agcgtacgat cgaattcctg
     1501 cagctcacgg taactgatgc cgtatttgca gtaccagcgt acggcccaca gaatgatgtc
     1561 acgctgaaaa tgccggcctt tgaatgggtt catgtgcagc tccatcagca aaaggggatg
     1621 ataagtttat caccaccgac tatttgcaac agtgccgttg atcgtgctat gatcgactga
     1681 tgtcatcagc ggtggagtgc aatgtcgtgc aatacgaatg gcgaaaagcc gagctcatcg
     1741 gtcagcttct caaccttggg gttacccccg gcggtgtgct gctggtccac agctccttcc
     1801 gtagcgtccg gcccctcgaa gatgggccac ttggactgat cgaggccctg cgtgctacgc
     1861 tgggtccggg agggacgctc gtcatgccct cgtggtcagg tctggacgac gagccgttcg
     1921 atcctgccac gtcgcccgtt acaccggacc ttggagttgt ctctgacaca ttctggcgcc
     1981 tgccaaatgt aaagcgcagc gcccatccat ttgcctttgc ggcagcgggg ccacaggcag
     2041 agcagatcat ctctgatcca ttgcccctgc caccttactc gcctgcaagc ccggtcgccc
     2101 gtgtccatga actcgatggg caggtacttc tcctcggcgt gggacacgat gccaacacga
     2161 cgctgcatct tgccgagttg atggcaaagg ttccctatgg ggtgccgaga cactgcacca
     2221 ttcttcagga tggcaagttg gtacgcgtcg attatctcga gaatgaccac tgctgtgagc
     2281 gctttgcctt ggcggacagg tggctcaagg agaagagcct tcagaaggaa ggtccagtcg
     2341 gtcatgcctt tgctcggttg atccgctccc gcgacattgt ggcgacagcc ctgggtcaac
     2401 tgggccgaga tccgttgatc ttcctgcatc cgccagaggc gggatgcgaa gaatgcgatg
     2461 ccgctcgcca gtcgattggc tgagctcatg agcggagaac gagatgacgt tggaggggca
     2521 aggtcgcgct gattgctggg gcaacacggg ggatccacta gttccatgag cgtcagaccc
     2581 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2641 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2701 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     2761 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2821 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2881 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     2941 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3001 agaaagcgcc acgcttcccg aggggagaaa ggcggacagg tatccggtaa gcggcagggt
     3061 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3121 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3181 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3241 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     3301 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcaacg cgtgcggccg
     3361 cggtacccgg ggatcctcta gagtcgacca ccaagggcac catctctgct tgggccaccc
     3421 cgttggccgc agccagctcg ctgagagccg tgaacgacag ggcgaacgcc agcccgccga
     3481 cggcgagggt tccgaccgct gcaactcccg gtgcaacctt gtcccggtct attctcttca
     3541 ctgcaccagc tccaatctgg tgtgaatgcc cctcgtctgt tcgcgcaggc ggggggctct
     3601 attcgtttgt cagcatcgaa agtagccaga tcagggatgc gttgcaaccg cgtatgccca
     3661 ggtcagaaga gtcgcacaag agttgcagac ccctggaaag aaaaatggcc agagggcgaa
     3721 aacaccctct gaccagcgga gcgggcgacg ggaatcgaac ccgcgtagct agtttggaag
     3781 aatgggtgtc tgccgaccac atatgggccg gtcaagatag gtttttaccc cctctcggct
     3841 gcatcctcta agtggaaaga aattgcaggt cgtagaagcg cgttgaagcc tgagagttgc
     3901 acaggagttg caacccggta gccttgttca cgacgagagg agacctagtt ggcacgtcgc
     3961 ggatggggat cgctgaagac tcagcgcagc gggaggatcc aagcctcata cgtcaacccg
     4021 caggacggtg tgaggtacta cgcgctgcag acctacgaca acaagatgga cgccgaagcc
     4081 tggctcgcgg gcgagaagcg gctcatcgag atggagacct ggacccctcc acaggaccgg
     4141 gcgaagaagg cagccgccag cgccatcacg ctggaggagt acacccggaa gtggctcgtg
     4201 gagcgcgacc tcgcagacgg caccagggat ctgtacagcg ggcacgcgga gcgccgcatc
     4261 tacccggtgc taggtgaagt ggcggtcaca gagatgacgc cagctctggt gcgtgcgtgg
     4321 tgggccggga tgggtaggaa gcacccgact gcccgccggc atgcctacaa cgtcctccgg
     4381 gcggtgatga acacagcggt cgaggacaag ctgatcgcag agaacccgtg ccggatcgag
     4441 cagaaggcag ccgatgagcg cgacgtagag gcgctgacgc ctgaggagct ggacatcgtc
     4501 gccgctgaga tcttcgagca ctaccggatc gcggcataca tcctggcgtg gacgagcctc
     4561 cggttcggag agctgatcga gcttcgccgc aaggacatcg tggacgacgg catgacgatg
     4621 aagctccggg tgcgccgtgg cgcttcccgc gtggggaaca agatcgtcgt tggcaacgcc
     4681 aagaccgtcc ggtcgaagcg tcctgtgacg gttccgcctc acgtcgcgga gatgatccga
     4741 gcgcacatga aggaccgtac gaagatgaac aagggccccg aggcattcct ggtgaccacg
     4801 acgcagggca accggctgtc gaagtccgcg ttcaccaagt cgctgaagcg tggctacgcc
     4861 aagatcggtc ggccggaact ccgcatccac gacctccgcg ctgtcggcgc tacgttcgcc
     4921 gctcaggcag gtgcgacgac caaggagctg atggcccgtc tcggtcacac gactcctagg
     4981 atggcgatga agtaccagat ggcgtctgag gcccgcgacg aggctatcgc tgaggcgatg
     5041 tccaagctgg ccaagacctc ctgaaacgca aaaagccccc ctcccaagga cactgagtcc
     5101 taaagagggg ggtttcttgt cagtacgcga agaaccacgc ctggccgcga gcgccagcac
     5161 cgccgctctg tgcggagacc tgggcaccag ccccgccgcc gccaggagca ttgccgttcc
     5221 cgccagaaat ctagacggtg accacaacgc gcccgctttg atcggggacg tcgaaatatt
     5281 ggatcgtcgg caccgtcacg gccgtgggag gcggcacgat ccgcgacgtg atgatcggcc
     5341 gcatccccac ggtgctgcgc agtgagctct acgccatccc ggcgttgatc tgtgcgttcg
     5401 cacgcacagg cccggtgtga gaagggtctc tgacgagcgg gagaactccc tatcagtgat
     5461 agagtttgtc ctccctatca gtgatagata ggcttaagga gtaatacgac tcactatagg
     5521 gagaaggagg ccattatgag cgccgacacg aagtggaccg aggccgacgt tcccgatcaa
     5581 tcaggccgcg tggccatc
//
