LOCUS       sequence-345588-        5738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0912:Dendra
FEATURES             Location/Qualifiers
     source          1..5738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     360..379
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        360..378
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1996..2019
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2276..3076
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3201..3789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3690..3709
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccgcgagc gccagcaccg ccgctctgtg cggagacctg ggcaccagcc ccgccgccgc
       61 caggagcatt gccgttcccg ccagaaatct agacggtgac cacaacgcgc ccgctttgat
      121 cggggacgtc gaaatattgg atcgtcggca ccgtcacggc cgtgggaggc ggcacgatcc
      181 gcgacgtgat gatcggccgc atccccacgg tgctgcgcag tgagctctac gccatcccgg
      241 cgttgatctg tgcgttcgca cgcacaggcc cggtgtgaga agggtctctg acgagcggga
      301 gaactcccta tcagtgatag agtttgtcct ccctatcagt gatagatagg cttaaggagt
      361 aatacgactc actataggga gaaggaggcc attatgagca atgcaatcga acgagtaggt
      421 gtcgtcggag ccggccagat gggcagcggc atcgccgagg tgtccgcccg cgcgggcgtc
      481 gacgtcctgg tctacgacac cactgacgcc ttcgtgacgg ccgggcgcaa ccgcatcacg
      541 aagtcgctgg agcgcgccgt gagcgcgggc aaggtcaccg agcgtgagaa ggacgaggcg
      601 ctgggccggc tgcgcttcac cacgagcctc gccgacatgg ccgaccgtca gctggtcatc
      661 gaggccgtga tcgaggacga gaacatcaag ggcgagatct tcgccgaact cgaccgcgtg
      721 atcaccgacc ccgacgcggt gctggcgtcg aacacgtcga gcatcccgat catgaagatc
      781 gcggcgtcca ccgagaaccc cgagcgcgtg ctgggcctgc acttcttcaa cccggtgccc
      841 gtcctgccac tcgtcgagtt ggtgtccaca ctcgtcacct ccgaggcggc ggccgaccgg
      901 gtcgagcagt tcgccggatc ggtgctgggc aagcaggtcg tgcggtgctc cgaccggtcc
      961 ggcttcgtgg tgaacttcct gctcgtgccg tacctgctct cggcaatccg catggtcgaa
     1021 ggtggcgtcg ccaccatcga ggacgtcgac aaggccgtgg tggccgggtt gtcgcacccg
     1081 atggggccgc tgcgcctgtc cgatctggta gggctcgaca cgctgaagct gatcgccgac
     1141 aagatgttcg acgagttcaa ggagcccctg tacgcggcgc cgccactgct gctgcgcatg
     1201 gtcgaggccg gccagctcgg caagaaaacg ggcaagggct tctataccta caagcttggt
     1261 gcggccgcgg gcaacacgcc tggcatcaat ctgatcaagg aggacatgcg tgtcaaggtg
     1321 catatggagg gcaacgtgaa cggtcacgcc ttcgtgatcg agggggaggg taagggtaag
     1381 ccttacgaag ggacccagac cgccaacctc accgtcaagg agggcgcccc tctcccattc
     1441 tcgtacgata tcctcactac cgcggtgcat tacgggaacc gcgtgttcac caagtacccc
     1501 gaggatatcc ctgactactt caagcagagc ttcccggagg gatactcctg ggagcggacg
     1561 atgaccttcg aagacaaggg tatctgtacg atccgcagcg acatctcgct ggaaggggac
     1621 tgtttcttcc agaacgtccg tttcaagggg accaactttc cgccgaacgg cccggtcatg
     1681 cagaagaaga ccctcaagtg ggagccgagc acggagaagc tgcacgttcg ggacggcctc
     1741 ctggtcggca acatcaacat ggcgctgctg ctggaaggcg gcggccacta cctatgcgac
     1801 ttcaagacca cgtacaaagc gaagaaggtc gtccagctgc ccgacgcgca cttcgtggac
     1861 caccgcatcg aaatcctggg gaacgattcg gactacaaca aggtcaagtt gtacgagcac
     1921 gctgtggcac gctatagccc gctcccgtcg caggtctggt tcgaaggcgg cggcggggag
     1981 aacctgtatt tccaggacta caaggacgac gacgacaagt gatttaaata accccttggg
     2041 gcctctaaac gggtcttgag gggttttttg ttaactagcg tacgatcgaa ttcctgcagc
     2101 tcacggtaac tgatgccgta tttgcagtac cagcgtacgg cccacagaat gatgtcacgc
     2161 tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca tcagcaaaag gggatgataa
     2221 gtttatcacc accgactatt tgcaacagtg ccgttgatcg tgctatgatc gactgatgtc
     2281 atcagcggtg gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc tcatcggtca
     2341 gcttctcaac cttggggtta cccccggcgg tgtgctgctg gtccacagct ccttccgtag
     2401 cgtccggccc ctcgaagatg ggccacttgg actgatcgag gccctgcgtg ctacgctggg
     2461 tccgggaggg acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc cgttcgatcc
     2521 tgccacgtcg cccgttacac cggaccttgg agttgtctct gacacattct ggcgcctgcc
     2581 aaatgtaaag cgcagcgccc atccatttgc ctttgcggca gcggggccac aggcagagca
     2641 gatcatctct gatccattgc ccctgccacc ttactcgcct gcaagcccgg tcgcccgtgt
     2701 ccatgaactc gatgggcagg tacttctcct cggcgtggga cacgatgcca acacgacgct
     2761 gcatcttgcc gagttgatgg caaaggttcc ctatggggtg ccgagacact gcaccattct
     2821 tcaggatggc aagttggtac gcgtcgatta tctcgagaat gaccactgct gtgagcgctt
     2881 tgccttggcg gacaggtggc tcaaggagaa gagccttcag aaggaaggtc cagtcggtca
     2941 tgcctttgct cggttgatcc gctcccgcga cattgtggcg acagccctgg gtcaactggg
     3001 ccgagatccg ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat gcgatgccgc
     3061 tcgccagtcg attggctgag ctcatgagcg gagaacgaga tgacgttgga ggggcaaggt
     3121 cgcgctgatt gctggggcaa cacgggggat ccactagttc catgagcgtc agaccccgta
     3181 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3241 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3301 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     3361 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3421 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3481 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3541 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3601 agcgccacgc ttcccgaggg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3661 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3721 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3781 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3841 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3901 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg cggccgcggt
     3961 acccggggat cctctagagt cgaccaccaa gggcaccatc tctgcttggg ccaccccgtt
     4021 ggccgcagcc agctcgctga gagccgtgaa cgacagggcg aacgccagcc cgccgacggc
     4081 gagggttccg accgctgcaa ctcccggtgc aaccttgtcc cggtctattc tcttcactgc
     4141 accagctcca atctggtgtg aatgcccctc gtctgttcgc gcaggcgggg ggctctattc
     4201 gtttgtcagc atcgaaagta gccagatcag ggatgcgttg caaccgcgta tgcccaggtc
     4261 agaagagtcg cacaagagtt gcagacccct ggaaagaaaa atggccagag ggcgaaaaca
     4321 ccctctgacc agcggagcgg gcgacgggaa tcgaacccgc gtagctagtt tggaagaatg
     4381 ggtgtctgcc gaccacatat gggccggtca agataggttt ttaccccctc tcggctgcat
     4441 cctctaagtg gaaagaaatt gcaggtcgta gaagcgcgtt gaagcctgag agttgcacag
     4501 gagttgcaac ccggtagcct tgttcacgac gagaggagac ctagttggca cgtcgcggat
     4561 ggggatcgct gaagactcag cgcagcggga ggatccaagc ctcatacgtc aacccgcagg
     4621 acggtgtgag gtactacgcg ctgcagacct acgacaacaa gatggacgcc gaagcctggc
     4681 tcgcgggcga gaagcggctc atcgagatgg agacctggac ccctccacag gaccgggcga
     4741 agaaggcagc cgccagcgcc atcacgctgg aggagtacac ccggaagtgg ctcgtggagc
     4801 gcgacctcgc agacggcacc agggatctgt acagcgggca cgcggagcgc cgcatctacc
     4861 cggtgctagg tgaagtggcg gtcacagaga tgacgccagc tctggtgcgt gcgtggtggg
     4921 ccgggatggg taggaagcac ccgactgccc gccggcatgc ctacaacgtc ctccgggcgg
     4981 tgatgaacac agcggtcgag gacaagctga tcgcagagaa cccgtgccgg atcgagcaga
     5041 aggcagccga tgagcgcgac gtagaggcgc tgacgcctga ggagctggac atcgtcgccg
     5101 ctgagatctt cgagcactac cggatcgcgg catacatcct ggcgtggacg agcctccggt
     5161 tcggagagct gatcgagctt cgccgcaagg acatcgtgga cgacggcatg acgatgaagc
     5221 tccgggtgcg ccgtggcgct tcccgcgtgg ggaacaagat cgtcgttggc aacgccaaga
     5281 ccgtccggtc gaagcgtcct gtgacggttc cgcctcacgt cgcggagatg atccgagcgc
     5341 acatgaagga ccgtacgaag atgaacaagg gccccgaggc attcctggtg accacgacgc
     5401 agggcaaccg gctgtcgaag tccgcgttca ccaagtcgct gaagcgtggc tacgccaaga
     5461 tcggtcggcc ggaactccgc atccacgacc tccgcgctgt cggcgctacg ttcgccgctc
     5521 aggcaggtgc gacgaccaag gagctgatgg cccgtctcgg tcacacgact cctaggatgg
     5581 cgatgaagta ccagatggcg tctgaggccc gcgacgaggc tatcgctgag gcgatgtcca
     5641 agctggccaa gacctcctga aacgcaaaaa gcccccctcc caaggacact gagtcctaaa
     5701 gaggggggtt tcttgtcagt acgcgaagaa ccacgcct
//
