LOCUS       sequence-345589-        6182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0933:Dendra
FEATURES             Location/Qualifiers
     source          1..6182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     954..973
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        954..972
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3034..3057
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3314..4114
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      4239..4827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4728..4747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggacgagcc tccggttcgg agagctgatc gagcttcgcc gcaaggacat cgtggacgac
       61 ggcatgacga tgaagctccg ggtgcgccgt ggcgcttccc gcgtggggaa caagatcgtc
      121 gttggcaacg ccaagaccgt ccggtcgaag cgtcctgtga cggttccgcc tcacgtcgcg
      181 gagatgatcc gagcgcacat gaaggaccgt acgaagatga acaagggccc cgaggcattc
      241 ctggtgacca cgacgcaggg caaccggctg tcgaagtccg cgttcaccaa gtcgctgaag
      301 cgtggctacg ccaagatcgg tcggccggaa ctccgcatcc acgacctccg cgctgtcggc
      361 gctacgttcg ccgctcaggc aggtgcgacg accaaggagc tgatggcccg tctcggtcac
      421 acgactccta ggatggcgat gaagtaccag atggcgtctg aggcccgcga cgaggctatc
      481 gctgaggcga tgtccaagct ggccaagacc tcctgaaacg caaaaagccc ccctcccaag
      541 gacactgagt cctaaagagg ggggtttctt gtcagtacgc gaagaaccac gcctggccgc
      601 gagcgccagc accgccgctc tgtgcggaga cctgggcacc agccccgccg ccgccaggag
      661 cattgccgtt cccgccagaa atctagacgg tgaccacaac gcgcccgctt tgatcgggga
      721 cgtcgaaata ttggatcgtc ggcaccgtca cggccgtggg aggcggcacg atccgcgacg
      781 tgatgatcgg ccgcatcccc acggtgctgc gcagtgagct ctacgccatc ccggcgttga
      841 tctgtgcgtt cgcacgcaca ggcccggtgt gagaagggtc tctgacgagc gggagaactc
      901 cctatcagtg atagagtttg tcctccctat cagtgataga taggcttaag gagtaatacg
      961 actcactata gggagaagga ggccattatg cgtctagcga cagacctcga gaccccccgc
     1021 cgcgtggcgg tgttgtcggt acacacctct ccgctggcgc agccgggcac cggcgacgcg
     1081 ggcggcatga acgtctacgt gttgcagacc gcgctgcaac tggcccggcg tggcgtcgag
     1141 gtcgaggtct tcaccagggc cacgtcgtcg gccgatgcgc cggtcgtgcc tgtggcgccc
     1201 ggtgtgctgg tgcgcaacgt cgtggccggc ccgttcgaag gcctcgacaa gaacgatctg
     1261 cccacgcagc tgtgcgcgtt caccgcgggt gtgctgcgcg ccgaggcgac ccacgagccc
     1321 ggctactacg acgtcgtgca ttcgcactac tggctgtccg gccaggtcgg gtggctggcg
     1381 cgcgaccgct gggccgtgcc gctggtgcac accgcgcaca cgctggccgc ggtcaagaac
     1441 gccgcactcg ccgcgggcga cgcacccgag ccgccgctgc gcgcggtggg cgaacaacag
     1501 gtggtcgacg aggccgaccg cctcatcgtg aacaccgaag tcgaagcgca gcaactggtc
     1561 tcgttgcaca atgccgaccg ctcacgcatc gacgtcgtgc accccggcgt cgatctcgac
     1621 gtgttcaccc ccggttcgcg cgacgcggcg cgcgcggtgt tcgggcttcc caccgaccag
     1681 aagatcgtgg cgttcgtggg ccgcatccag ccgctcaagg cccccgacat cctgctgcgt
     1741 gccgcggcga aactgcccgg cgtgcgcgtg ctgatcgccg gtggaccctc cggatcggga
     1801 cttgcccaac cggacacgct ggttcggctc gccgacgaac tgggtatcag tgaccgggtg
     1861 acgttcctcc cgccgcagag ccgcgaacaa ctggtcaacg tgtaccgggc ggccgatctg
     1921 gtcgcggtgc cgagctactc cgagtcgttc ggcctggtcg ccgtcgaggc gcaggcgtgc
     1981 ggcacgcccg tcgtcgccgc ggccgtcggc ggactgccgg tcgcggtggc cgacggcgtc
     2041 agcggggcac tcgtcgacgg ccacgacatc ggcgactggg ccgacaccat cagcgaggtg
     2101 ctcgaccgcg agcccgccgc gctgagccgc gcctccgccg aacacgccgc tcagttctcg
     2161 tgggcgcaca ccgtcgacgc gctgctcgcc agctacagcc gggccatgag tgactaccgg
     2221 gcccgtcatc ccagacccgc cgcgcggcgt tccggacgcc ggttctcgat gcgcagggga
     2281 gtacgcacga agcttggtgc ggccgcgggc aacacgcctg gcatcaatct gatcaaggag
     2341 gacatgcgtg tcaaggtgca tatggagggc aacgtgaacg gtcacgcctt cgtgatcgag
     2401 ggggagggta agggtaagcc ttacgaaggg acccagaccg ccaacctcac cgtcaaggag
     2461 ggcgcccctc tcccattctc gtacgatatc ctcactaccg cggtgcatta cgggaaccgc
     2521 gtgttcacca agtaccccga ggatatccct gactacttca agcagagctt cccggaggga
     2581 tactcctggg agcggacgat gaccttcgaa gacaagggta tctgtacgat ccgcagcgac
     2641 atctcgctgg aaggggactg tttcttccag aacgtccgtt tcaaggggac caactttccg
     2701 ccgaacggcc cggtcatgca gaagaagacc ctcaagtggg agccgagcac ggagaagctg
     2761 cacgttcggg acggcctcct ggtcggcaac atcaacatgg cgctgctgct ggaaggcggc
     2821 ggccactacc tatgcgactt caagaccacg tacaaagcga agaaggtcgt ccagctgccc
     2881 gacgcgcact tcgtggacca ccgcatcgaa atcctgggga acgattcgga ctacaacaag
     2941 gtcaagttgt acgagcacgc tgtggcacgc tatagcccgc tcccgtcgca ggtctggttc
     3001 gaaggcggcg gcggggagaa cctgtatttc caggactaca aggacgacga cgacaagtga
     3061 tttaaataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt aactagcgta
     3121 cgatcgaatt cctgcagctc acggtaactg atgccgtatt tgcagtacca gcgtacggcc
     3181 cacagaatga tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg cagctccatc
     3241 agcaaaaggg gatgataagt ttatcaccac cgactatttg caacagtgcc gttgatcgtg
     3301 ctatgatcga ctgatgtcat cagcggtgga gtgcaatgtc gtgcaatacg aatggcgaaa
     3361 agccgagctc atcggtcagc ttctcaacct tggggttacc cccggcggtg tgctgctggt
     3421 ccacagctcc ttccgtagcg tccggcccct cgaagatggg ccacttggac tgatcgaggc
     3481 cctgcgtgct acgctgggtc cgggagggac gctcgtcatg ccctcgtggt caggtctgga
     3541 cgacgagccg ttcgatcctg ccacgtcgcc cgttacaccg gaccttggag ttgtctctga
     3601 cacattctgg cgcctgccaa atgtaaagcg cagcgcccat ccatttgcct ttgcggcagc
     3661 ggggccacag gcagagcaga tcatctctga tccattgccc ctgccacctt actcgcctgc
     3721 aagcccggtc gcccgtgtcc atgaactcga tgggcaggta cttctcctcg gcgtgggaca
     3781 cgatgccaac acgacgctgc atcttgccga gttgatggca aaggttccct atggggtgcc
     3841 gagacactgc accattcttc aggatggcaa gttggtacgc gtcgattatc tcgagaatga
     3901 ccactgctgt gagcgctttg ccttggcgga caggtggctc aaggagaaga gccttcagaa
     3961 ggaaggtcca gtcggtcatg cctttgctcg gttgatccgc tcccgcgaca ttgtggcgac
     4021 agccctgggt caactgggcc gagatccgtt gatcttcctg catccgccag aggcgggatg
     4081 cgaagaatgc gatgccgctc gccagtcgat tggctgagct catgagcgga gaacgagatg
     4141 acgttggagg ggcaaggtcg cgctgattgc tggggcaaca cgggggatcc actagttcca
     4201 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4261 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4321 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4381 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4441 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4501 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4561 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4621 cagcgtgagc tatgagaaag cgccacgctt cccgagggga gaaaggcgga caggtatccg
     4681 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4741 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4801 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4861 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4921 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     4981 aacgcgtgcg gccgcggtac ccggggatcc tctagagtcg accaccaagg gcaccatctc
     5041 tgcttgggcc accccgttgg ccgcagccag ctcgctgaga gccgtgaacg acagggcgaa
     5101 cgccagcccg ccgacggcga gggttccgac cgctgcaact cccggtgcaa ccttgtcccg
     5161 gtctattctc ttcactgcac cagctccaat ctggtgtgaa tgcccctcgt ctgttcgcgc
     5221 aggcgggggg ctctattcgt ttgtcagcat cgaaagtagc cagatcaggg atgcgttgca
     5281 accgcgtatg cccaggtcag aagagtcgca caagagttgc agacccctgg aaagaaaaat
     5341 ggccagaggg cgaaaacacc ctctgaccag cggagcgggc gacgggaatc gaacccgcgt
     5401 agctagtttg gaagaatggg tgtctgccga ccacatatgg gccggtcaag ataggttttt
     5461 accccctctc ggctgcatcc tctaagtgga aagaaattgc aggtcgtaga agcgcgttga
     5521 agcctgagag ttgcacagga gttgcaaccc ggtagccttg ttcacgacga gaggagacct
     5581 agttggcacg tcgcggatgg ggatcgctga agactcagcg cagcgggagg atccaagcct
     5641 catacgtcaa cccgcaggac ggtgtgaggt actacgcgct gcagacctac gacaacaaga
     5701 tggacgccga agcctggctc gcgggcgaga agcggctcat cgagatggag acctggaccc
     5761 ctccacagga ccgggcgaag aaggcagccg ccagcgccat cacgctggag gagtacaccc
     5821 ggaagtggct cgtggagcgc gacctcgcag acggcaccag ggatctgtac agcgggcacg
     5881 cggagcgccg catctacccg gtgctaggtg aagtggcggt cacagagatg acgccagctc
     5941 tggtgcgtgc gtggtgggcc gggatgggta ggaagcaccc gactgcccgc cggcatgcct
     6001 acaacgtcct ccgggcggtg atgaacacag cggtcgagga caagctgatc gcagagaacc
     6061 cgtgccggat cgagcagaag gcagccgatg agcgcgacgt agaggcgctg acgcctgagg
     6121 agctggacat cgtcgccgct gagatcttcg agcactaccg gatcgcggca tacatcctgg
     6181 cg
//
