LOCUS       sequence-345590-        5738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0943:Dendra
FEATURES             Location/Qualifiers
     source          1..5738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     717..736
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        717..735
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2353..2376
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2633..3433
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3558..4146
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4047..4066
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcctggtga ccacgacgca gggcaaccgg ctgtcgaagt ccgcgttcac caagtcgctg
       61 aagcgtggct acgccaagat cggtcggccg gaactccgca tccacgacct ccgcgctgtc
      121 ggcgctacgt tcgccgctca ggcaggtgcg acgaccaagg agctgatggc ccgtctcggt
      181 cacacgactc ctaggatggc gatgaagtac cagatggcgt ctgaggcccg cgacgaggct
      241 atcgctgagg cgatgtccaa gctggccaag acctcctgaa acgcaaaaag cccccctccc
      301 aaggacactg agtcctaaag aggggggttt cttgtcagta cgcgaagaac cacgcctggc
      361 cgcgagcgcc agcaccgccg ctctgtgcgg agacctgggc accagccccg ccgccgccag
      421 gagcattgcc gttcccgcca gaaatctaga cggtgaccac aacgcgcccg ctttgatcgg
      481 ggacgtcgaa atattggatc gtcggcaccg tcacggccgt gggaggcggc acgatccgcg
      541 acgtgatgat cggccgcatc cccacggtgc tgcgcagtga gctctacgcc atcccggcgt
      601 tgatctgtgc gttcgcacgc acaggcccgg tgtgagaagg gtctctgacg agcgggagaa
      661 ctccctatca gtgatagagt ttgtcctccc tatcagtgat agataggctt aaggagtaat
      721 acgactcact atagggagaa ggaggccatt atgtcgagaa tcgcgatcat cggcggcgga
      781 agcatgggtg aggcactgtt gtccggcctg ctccgggccg gcaggcaggt caaggacatg
      841 gtcgttgcgg agaagttccc cgagcgtgcc aagtacctgg cggacaagta ctccgtgcgc
      901 gtcacgtcgg ttgccgacgc cgccgagaat gcccactacg tcgtcgtcgc cgtcaagccc
      961 gccgacgtcg agaaggtgat cggcgagatc gccgaggccg ctgccaacgc cgagaccgat
     1021 acggccgagc aggttttcgt caccatcgcg gccggcgtcg gcaccgcgta ctacgaggcc
     1081 aagctgcccg cgggctcgcc ggtgatccgg gtgatgccca atgcgccggt gatcgtcggc
     1141 ggcggcgtca gcgcgctggc acccggtcgc ttcgcgacgc ccgagcagct caaggaggtc
     1201 tcgacgctgt tcgacgcggt cggtggcgtc atcacggtct ccgagtccca actggacgcc
     1261 gtcacggccc tgtccggttc gggcccggcg tacttcttcc tgatggccga ggcgatggtc
     1321 gacgccgggg tggctgtcgg gctgtcgcgt gccgtggcga ccgatctggt tgcccagacg
     1381 atggccggtt cggccgcgat gctgctggac cgcctggatc aggtgaacgc cgcgggaggc
     1441 gcggcgctgg acaccacacc ggcccagttg cgggccaccg tcacctcgcc ggccggcaca
     1501 accgccgctg gtctgcgcga actcgagcgg ggcgggctgc gcgccgcggt ggcaaatgcc
     1561 gtggaagccg cgaaaacgcg ctctgagcag ctaggaatta catcggagaa gcttggtgcg
     1621 gccgcgggca acacgcctgg catcaatctg atcaaggagg acatgcgtgt caaggtgcat
     1681 atggagggca acgtgaacgg tcacgccttc gtgatcgagg gggagggtaa gggtaagcct
     1741 tacgaaggga cccagaccgc caacctcacc gtcaaggagg gcgcccctct cccattctcg
     1801 tacgatatcc tcactaccgc ggtgcattac gggaaccgcg tgttcaccaa gtaccccgag
     1861 gatatccctg actacttcaa gcagagcttc ccggagggat actcctggga gcggacgatg
     1921 accttcgaag acaagggtat ctgtacgatc cgcagcgaca tctcgctgga aggggactgt
     1981 ttcttccaga acgtccgttt caaggggacc aactttccgc cgaacggccc ggtcatgcag
     2041 aagaagaccc tcaagtggga gccgagcacg gagaagctgc acgttcggga cggcctcctg
     2101 gtcggcaaca tcaacatggc gctgctgctg gaaggcggcg gccactacct atgcgacttc
     2161 aagaccacgt acaaagcgaa gaaggtcgtc cagctgcccg acgcgcactt cgtggaccac
     2221 cgcatcgaaa tcctggggaa cgattcggac tacaacaagg tcaagttgta cgagcacgct
     2281 gtggcacgct atagcccgct cccgtcgcag gtctggttcg aaggcggcgg cggggagaac
     2341 ctgtatttcc aggactacaa ggacgacgac gacaagtgat ttaaataacc ccttggggcc
     2401 tctaaacggg tcttgagggg ttttttgtta actagcgtac gatcgaattc ctgcagctca
     2461 cggtaactga tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga
     2521 aaatgccggc ctttgaatgg gttcatgtgc agctccatca gcaaaagggg atgataagtt
     2581 tatcaccacc gactatttgc aacagtgccg ttgatcgtgc tatgatcgac tgatgtcatc
     2641 agcggtggag tgcaatgtcg tgcaatacga atggcgaaaa gccgagctca tcggtcagct
     2701 tctcaacctt ggggttaccc ccggcggtgt gctgctggtc cacagctcct tccgtagcgt
     2761 ccggcccctc gaagatgggc cacttggact gatcgaggcc ctgcgtgcta cgctgggtcc
     2821 gggagggacg ctcgtcatgc cctcgtggtc aggtctggac gacgagccgt tcgatcctgc
     2881 cacgtcgccc gttacaccgg accttggagt tgtctctgac acattctggc gcctgccaaa
     2941 tgtaaagcgc agcgcccatc catttgcctt tgcggcagcg gggccacagg cagagcagat
     3001 catctctgat ccattgcccc tgccacctta ctcgcctgca agcccggtcg cccgtgtcca
     3061 tgaactcgat gggcaggtac ttctcctcgg cgtgggacac gatgccaaca cgacgctgca
     3121 tcttgccgag ttgatggcaa aggttcccta tggggtgccg agacactgca ccattcttca
     3181 ggatggcaag ttggtacgcg tcgattatct cgagaatgac cactgctgtg agcgctttgc
     3241 cttggcggac aggtggctca aggagaagag ccttcagaag gaaggtccag tcggtcatgc
     3301 ctttgctcgg ttgatccgct cccgcgacat tgtggcgaca gccctgggtc aactgggccg
     3361 agatccgttg atcttcctgc atccgccaga ggcgggatgc gaagaatgcg atgccgctcg
     3421 ccagtcgatt ggctgagctc atgagcggag aacgagatga cgttggaggg gcaaggtcgc
     3481 gctgattgct ggggcaacac gggggatcca ctagttccat gagcgtcaga ccccgtagaa
     3541 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3601 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3661 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     3721 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3781 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3841 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3901 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3961 gccacgcttc ccgaggggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4021 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4081 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4141 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4201 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     4261 tgagctgata ccgctcgccg cagccgaacg accgagcgca acgcgtgcgg ccgcggtacc
     4321 cggggatcct ctagagtcga ccaccaaggg caccatctct gcttgggcca ccccgttggc
     4381 cgcagccagc tcgctgagag ccgtgaacga cagggcgaac gccagcccgc cgacggcgag
     4441 ggttccgacc gctgcaactc ccggtgcaac cttgtcccgg tctattctct tcactgcacc
     4501 agctccaatc tggtgtgaat gcccctcgtc tgttcgcgca ggcggggggc tctattcgtt
     4561 tgtcagcatc gaaagtagcc agatcaggga tgcgttgcaa ccgcgtatgc ccaggtcaga
     4621 agagtcgcac aagagttgca gacccctgga aagaaaaatg gccagagggc gaaaacaccc
     4681 tctgaccagc ggagcgggcg acgggaatcg aacccgcgta gctagtttgg aagaatgggt
     4741 gtctgccgac cacatatggg ccggtcaaga taggttttta ccccctctcg gctgcatcct
     4801 ctaagtggaa agaaattgca ggtcgtagaa gcgcgttgaa gcctgagagt tgcacaggag
     4861 ttgcaacccg gtagccttgt tcacgacgag aggagaccta gttggcacgt cgcggatggg
     4921 gatcgctgaa gactcagcgc agcgggagga tccaagcctc atacgtcaac ccgcaggacg
     4981 gtgtgaggta ctacgcgctg cagacctacg acaacaagat ggacgccgaa gcctggctcg
     5041 cgggcgagaa gcggctcatc gagatggaga cctggacccc tccacaggac cgggcgaaga
     5101 aggcagccgc cagcgccatc acgctggagg agtacacccg gaagtggctc gtggagcgcg
     5161 acctcgcaga cggcaccagg gatctgtaca gcgggcacgc ggagcgccgc atctacccgg
     5221 tgctaggtga agtggcggtc acagagatga cgccagctct ggtgcgtgcg tggtgggccg
     5281 ggatgggtag gaagcacccg actgcccgcc ggcatgccta caacgtcctc cgggcggtga
     5341 tgaacacagc ggtcgaggac aagctgatcg cagagaaccc gtgccggatc gagcagaagg
     5401 cagccgatga gcgcgacgta gaggcgctga cgcctgagga gctggacatc gtcgccgctg
     5461 agatcttcga gcactaccgg atcgcggcat acatcctggc gtggacgagc ctccggttcg
     5521 gagagctgat cgagcttcgc cgcaaggaca tcgtggacga cggcatgacg atgaagctcc
     5581 gggtgcgccg tggcgcttcc cgcgtgggga acaagatcgt cgttggcaac gccaagaccg
     5641 tccggtcgaa gcgtcctgtg acggttccgc ctcacgtcgc ggagatgatc cgagcgcaca
     5701 tgaaggaccg tacgaagatg aacaagggcc ccgaggca
//
