LOCUS       sequence-345621-       11832 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSOUP helper plasmid needed for Agrobacterium-mediated plant 
            transformation - Expression of GOXL genes tagged with Citrine under 
            RUBY promoter for transgene complementation of ruby-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11832)
  AUTHORS   Sola K, Dean GH, Li Y, Lohmann J, Movahedan M, Gilchrist EJ, Adams 
            KL, Haughn GW
  TITLE     Expression Patterns and Functional Characterisation of Arabidopsis 
            GALACTOSE OXIDASE-LIKE (GOXL) Genes Suggest Specialised Roles for 
            Galactose Oxidases in Plants.
  JOURNAL   Plant Cell Physiol. 2021 May 27. pii: 6286013. doi: 
            10.1093/pcp/pcab073.
  PUBMED    34042158
REFERENCE   2  (bases 1 to 11832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2021 May 27. pii: 6286013. doi: 10.1093/pcp/pcab073."
COMMENT     Sequence Label: pGreenII 0229/ProRUBY:GOXL1-Citrine:NOSt
FEATURES             Location/Qualifiers
     source          1..11832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    656..678
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(702..954)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(974..1525)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1567..1746)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1978..2000
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1992..2009
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1993..2009
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2019..2038
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2019..2037
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8559..8575)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8559..8575)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(8598..8600,8601..9311)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8640..8661)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8901..8920)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9248..9269
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      9353..9605
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(9624..9644)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9624..9642)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9663..9679)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9663..9679)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9676..9698)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9687..9703
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9711..9717,9718..9735,9736..9741))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9756..9777
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9894..9911)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10016..10040
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      10191..10626
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     primer_bind     complement(10906..10925)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             10917..11732
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             10917..11732
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(10990..11009)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatga gatcttggca
      661 ggatatattg tggtgtaacg ttatcagctt gcatgccggt cgatctagta acatagatga
      721 caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta
      781 aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta
      841 catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag
      901 accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatctgcttg
      961 actctagggg tcatcagatt tcggtgacgg gcaggaccgg acggggcggc accggcaggc
     1021 tgaagtccag ctgccagaaa cccacgtcat gccagttccc gtgcttgaag ccggccgccc
     1081 gcagcatgcc gcggggggca tatccgagcg cctcgtgcat gcgcacgctc gggtcgttgg
     1141 gcagcccgat gacagcgacc acgctcttga agccctgtgc ctccagggac ttcagcaggt
     1201 gggtgtagag cgtggagccc agtcccgtcc gctggtggcg gggggagacg tacacggtcg
     1261 actcggccgt ccagtcgtag gcgttgcgtg ccttccaggg acccgcgtag gcgatgccgg
     1321 cgacctcgcc gtccacctcg gcgacgagcc agggatagcg ctcccgcaga cggacgaggt
     1381 cgtccgtcca ctcctgcggt tcctgcggct cggtacggaa gttgaccgtg cttgtctcga
     1441 tgtagtggtt gacgatggtg cagaccgccg gcatgtccgc ctcggtggca cggcggatgt
     1501 cggccgggcg tcgttctggg ctcatggtag atcccctcga tcgagttgag agtgaatatg
     1561 agactctaat tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga
     1621 aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga
     1681 cgtatgtgct tagctcatta aactccagaa acccgcggct cagtggctcc ttcaacgttg
     1741 cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac
     1801 gtgactccct taattctccg ctcatgatcg ataacattaa cgcttacaat ttccattcgc
     1861 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     1921 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     1981 agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg
     2041 aattgggtac cgctaaccca atctcaatcg aaccggacca aattagaatg aaatggtttt
     2101 agtgagttac atggttctta atttgactgg atgaaattaa ttaaaggcac tatatagatg
     2161 aaactaaaac tacaagaaca ttgctctaac ttttacacaa ctttaaaaaa aaaaaaaaaa
     2221 aaaatcgtgg aatgttgatt catttgacct ttattctgga ttgaaagtga tgcaaaaatg
     2281 actttatcta ataaataaaa aaaagaagac tttatctgat gtagtgaaat tcttgtattt
     2341 gttgatgcaa tgatgctatg gattgttttc aaatgtttca attacgtatc aaattatttt
     2401 atttgtacca acacatgcaa gtatgcaacc atggcctcgt cgtaaagaac tttgggcaac
     2461 atacaaattt gggcaaacat ctataacaca ttggattttt ttgatcgatt taatttgatt
     2521 cttggaatta ctattacctt cttcttttaa aaattcagta agatatgtgt taccgtgtga
     2581 aagtctatat gtgtgggaac gaaaggtttg aagacgagta agaaagtaga agagcacaat
     2641 gcgaaacttt attggtatac gtaactgttt attgacgaca tttgtaggaa caaaaattgc
     2701 aagataacac attttttagt gtttgctttg cttgtttata gtaaccggta ggcagtggcg
     2761 gaggcagaat aattagatac aggggtcact ctttttgtaa gccaaatacc aaaaaaaaaa
     2821 aattaagaaa aatgataata tcctcataaa tgataatttc aaaaaaaaat atatatatga
     2881 gaaagaaaaa aactgttcta gtaaagtaaa cattaaatct aaagtaattt atactgtgtt
     2941 aaacaataat aatgagatta acttagttta atagacttat ttttttattt ataaaattaa
     3001 ttgggttaat ttgttgtagg tttaatattt tttgttagac tatatttgaa tatatattga
     3061 tgggggtcaa taatattttg ggaaagggtc aatttataaa aattaacaaa acctaaagta
     3121 attttctaaa atccataggg gtcaattgac ccccatgatt atacactccc tccgccactg
     3181 ccggtagggt ttgatgtctt ggattgttaa gttttaaagt tttgtctcat gatctatgta
     3241 agtgcttaag ccggcgctag cataaattga acctttaatc agaatgtagt agttgacctg
     3301 gatggatgac acttttatgc gagatgggtc gagcgacgct aaccattagg ccctcgatcg
     3361 gcagtcggca gatataactt agatacagtg gatagtgaca tatcctctta ataaatatgt
     3421 tttaaatgtt agattaactt atgacctaaa gaaaagaagt tagattaaca ttttttagaa
     3481 attattattt cggataggtt taataagcat aagtcagcga aacttataag gtatttattt
     3541 acaattataa aatcaacagc ttccaaacaa aatctttttt ctttttgact tcttaaaaca
     3601 aaatctcata ctaaccgatt taacattatg tttgtgaata atatggtaac gcgaatgatt
     3661 agcaacataa aaaacaaaga gtcaactaac cgcattcatg aatggaatgg aatgtcacgg
     3721 tcaatcatta gatttggtgt tacggaatac atatttatta aatgtatatg aagaaaaaag
     3781 catggccaat aataatgtaa ataaattggc tcaacatgcg atgtatgtac ataatttaga
     3841 gtggaaaatg aaaagatata tacaatgtgt attaatatag atgtacgcag ttgtataaaa
     3901 ttgatggaca tgatttaggt ttttttttaa aaaattgatg gacatttttc aaatatcaaa
     3961 atgtttaatt tatattttct acatataatt gaaattaaac aaaggagata taagttctac
     4021 ataattttac tttgtatcgt tggaacaaac acgtacagta gtattatttt atatatgtgc
     4081 atgtatgttt tgcgttcctt taaaaatcaa atacatggga agataacatg caacgaacca
     4141 gatgatacag cgcctgatga gaggtcaaaa acgtaacgag tttgacttgt ataatattaa
     4201 tatgtagttg catgtttgat gttgacacag catcgccatc attaattcgt tctctgcagt
     4261 ctaccgaact aattaatctc aaataatcga tgagtacata tatgttaagt ccgtcgaggc
     4321 taatgtataa ttcaataata taaatatgtt cattcgagga tatagttcat attattatat
     4381 atctgtttaa aattattgac tccaatcatc ctataaacta agagggttga caccgcagca
     4441 aagaaaaaaa taaaatatac cataattcat atatttgcac aatctgaatt ataatggaaa
     4501 ttaagtttgc tcaaacaaaa aaaaagggtt ggctccatat acgaaattgt taatttgtaa
     4561 ttttgtagga cttctaatta aaaaaatata tatcaataaa tgattatttt atatatgtca
     4621 attatatctg atcattaatt aattaattgt ttcgaaaata attaattaat tttgaaattt
     4681 aaattaaagc aatattaaat gtaaaaattt taaattatat taaactagaa atttattgtg
     4741 agcatttttg tgtatagatc aagatatgat gaatcttgtg ggattttttt tattctataa
     4801 caggtgttgt ttcatatgct acgcattttt atatatttaa ccaaacataa tgacaagttg
     4861 acaacgacta gtatttcttc ccatttagat gataagatta gtccaaatac tccaaaccgt
     4921 ctgttggatt aagaatgacc ggaatacgat tactccaaaa cgcatatggg tttactggtc
     4981 gagaatatac ctttggatta agatcatgta gagttatttc attacgattt tgatttcttt
     5041 tggttattgt gaatatgttt tttactttct tttttctgtt agagatcatt tgtgaatatg
     5101 ttaagtggta agtgtacaat ttttcatatt ttgaatcgag tatcataaat atcattgtac
     5161 acataccagt ttatttattt cgtcctgata ttaacaagta acaacgtcaa ttttgagatc
     5221 aattatttga aaatatagaa aactgaaagt attaaccaat ttagtttgat tctaattaga
     5281 gtcatcgtat tgtgctagta atcggtgacg ggaaccacat aaaacgacag tttatcaaca
     5341 taaaaaaaaa tcacaaacac atagaagact ctagttttgt tataaacttg tgctaaataa
     5401 ttaacacgat acataatact cttcattatt atatatactt ttcatgtata ataatgcatg
     5461 gacattacac aaatggaatg aatagtacaa ttgtatgtgc atgaatgttt ttctaaaaac
     5521 tgacatccgt ttgtatgcta ttttatcctt gtttgttatt taatagtaga aaacttgcga
     5581 ggaccaaaat aattagcatg caaagaacca gaagaaatcg tgagaactaa aacgtaaaga
     5641 actcttgtga cttgtataat atatagtttg tatgcatgtt tgatgttgac acaacatatc
     5701 tccatcatta attcgtccct tgcagtctct caaacttatt aatctcaaat aatcgatgtt
     5761 tatagattaa gtccctcgag actaataatg taaatccaat gatacaatgt ggttccttcg
     5821 acgacgaatc atattctgat ttctgtatat ctgtaatata aagtacgatt attattatat
     5881 ttgaatacaa tcatacttaa taaaatattg tgtcatatat atttatctcc ataaatgttt
     5941 ctcttgactg aaatgtgaat acccaaacat aaaaacatat acagtaaata ctttgttcaa
     6001 ttcccattaa atttctcttt gaatctaaaa tcagacatag aaatactgtt gtttagagaa
     6061 agattttgaa tatatagaac attaaatcgc cgcagaactt tgttgtttag agaaagattt
     6121 taaatatgat aagaactcaa ctaaagaaaa agaaaaaaaa ttatgacgtc tcaatttaga
     6181 gttttattgg ttcttttgta cagttctgta atgttaggaa tcgttttata cacaaaggta
     6241 acttaaatct ttcaaacgtc tacggtagtg gacggtcatt aaaatcagag ttaagaaaac
     6301 gaacaaacca aatttaatca tatcttatac actcgagacc ttgattatat attactcttt
     6361 tatatattaa cagaactaga aataaaaact atacattatt agaatatcgt aacgtatact
     6421 aatatcccat cgaccagtat ataaacgaca caagtcacag tacattctca tcacatacca
     6481 caaaatatct cataaagatc aaataaacac taaacctgaa agcaaaaaag ggcccatgaa
     6541 aaagtcaaca agactcttat ggctattatc catcattgtt ttggtggcag ccgtttctaa
     6601 ggcagtcgca gaggtagaca acgatgatga tgacgataat acttcattgg aaggcatgac
     6661 gacggctaaa cgtgaaaccc tcgaagttga ggatcatact tcattggaag gcatggttaa
     6721 acgtgaagcc ctcgaagtaa aaccaccaaa agctggcaaa ggcaaaggca aaggtaaagg
     6781 tcgtggcacc gttgccgcag gacctgagat gaattggcct ggacaatggg agttattcat
     6841 gaagaattct ggtgtttcag ccatgcacgc aattttgatg cctttgatca ataaagttca
     6901 gttttacgac gcaaccattt ggagaatttc acaaatcaaa ttgcctccag gcgtaccttg
     6961 ccatgttttt gacgccaaaa aaaataaagt tgattgttgg gctcactctg tcctcgtcga
     7021 tattaacacc ggagacatca agcctcttgc ggtaacaaaa ttactaatta ttactccctc
     7081 cgttcctttt ttatataacg ttttagttaa aatcacgcag attaagaaat tacaaatata
     7141 attcatttta cacattagta attgttgatc tgatggattt aatggaaaca tgacagctta
     7201 caacagacac atggtgttct tctggaggtt taaccgtaaa cggtactttg gtaagtactg
     7261 gaggattcca aggaggagca aacacggcaa ggtacctatc cacatgcgaa aactgtgtat
     7321 ggattgaata tccaaaggca ctcgctgcaa ggagatggta ctcaacacaa gccactcttc
     7381 ctgatggaac tttcatcgtt gttggaggcc gtgacgctct caactacgag tatatccttc
     7441 cggaaggaca aaacaacaag aaactctatg actcacaact tcttagacaa acagatgatc
     7501 cagaagaaaa caatctttac ccttttgttt ggcttaacac tgatggtaac ctcttcattt
     7561 tcgccaacaa ccgatccatt cttcttagcc cgaaaacaaa caaagtgctc aaagagttcc
     7621 ctcaacttcc cggtggtgcc cgtaactacc ctggttccgc ctcctcggct cttctcccca
     7681 tccgcctcta cgtccaaaac cccgctatca tccccgctga tgtcctagtt tgcggtggtg
     7741 caaaacaaga cgcttacttc cgtgcagaga ggttaaagat ttacgattgg gccttgaaag
     7801 attgtgcaag gttaaacatc aatagtgcta agcctgtatg gaaaacagag accatgccaa
     7861 cgtcacgtgt gatgagtgat actgtgatcc ttcctaatgg agaaattctt atcattaatg
     7921 gtgcaaaacg tggctcctct ggttggcact tagccaaaga accaaatttt gctccactct
     7981 tgtacaaacc taacaaacct ttaggccaac gtttcaaaga actcgctcct tccactatcc
     8041 ctcgtgtgta ccattccatc gcaatcgctc ttcctgatgg taaagttctc gtcggtggaa
     8101 gcaacaccaa caacggatac caattcaacg ttgagtaccc aaccgaactc cgcatcgaga
     8161 aattctcacc accttacctt gatccagctc tagccaatat gagaccaaga atcgtaaaca
     8221 ccgcaacacc aaaacagatt aagtatggac aaatgtttga cgtaaagatc gagcttaaac
     8281 aacaaaacgt agcaaaggag aatgtaatgg tgacaatgtt ggcaccatca tttactacac
     8341 atagtgtatc aatgaacatg aggttgctta tgttgggtat taacaatgtg aagaatgttg
     8401 gtggcgataa tcatcaaatc caagccgttg ctcctcctag tggaaaactt gctcctcctg
     8461 gttattatct tctctttgcc gtctacaatg gtgtccctag cgttggagaa tggattcaga
     8521 ttgtcggaag cttgatatcg aattcctgca gcccggggga tccactagtt ctagagcctg
     8581 gaggtggagg tggagctgtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     8641 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     8701 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     8761 tgccctggcc caccctcgtg accaccttcg gctacggcct gatgtgcttc gcccgctacc
     8821 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     8881 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     8941 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     9001 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     9061 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     9121 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     9181 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
     9241 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     9301 agctgtacaa ggatcctgct ggtgctgctg cgcctctagc ataagcggcc gcgatcgttc
     9361 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     9421 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     9481 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     9541 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     9601 agatcgagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     9661 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     9721 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     9781 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     9841 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9901 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9961 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgaa ggccttgaca
    10021 ggatatattg gcgggtaaac taagtcgctg tatgtgtttg tttgagatct aattccgggg
    10081 atcggaaatc cagaagcccg agaggttgcc gcctttcggg ctttttcttt ttcaaaaaaa
    10141 aaaatttata aaacgatctg ttgcggccgg ccgccgggtt gtgggcaaag gcgctcgacg
    10201 gtgggcaacc gcttgcggtt gtccacgggc ggagccggtg cgcgtagcgc attgtccaca
    10261 agccaagggc gaccaataat tgatatatat attcataatt gaaaagctaa ttgaacatac
    10321 tacttgctgt aactacttgc cggagcgagg ggtgtttgca agctgttgat ctgaaagggc
    10381 tattagcgtt ctcacgtgcc tttttgatta gcgatttcac gtgaccttat tagcgatttc
    10441 acgtactccg attagcgatt tcacgtaccc tgattagcga tttcacgtgg atagtttttg
    10501 gagcgggccg gaaagccccg tgaatcaagg ctttgcgggg cattagcggt ttcacgtgga
    10561 taactaccct ctatccacag gcttccgggg ataaaaaagc ccgctcgacg gcgggctgtt
    10621 ggatggggat cgcctgaatc gccccatcat ccagccagaa agtgagggag ccacggttga
    10681 tgagagcttt gttgtaggtg gaccagttgg tgattttgaa cttttgcttt gccacggaac
    10741 ggtctgcgtt gtcgggaaga tgcgtgatct gatccttcaa ctcagcaaaa gttcgattta
    10801 ttcaacaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
    10861 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
    10921 gccatattca acgggaaacg tcttgctcaa ggccgcgatt aaattccaac atggatgctg
    10981 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctacc
    11041 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
    11101 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
    11161 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
    11221 cagggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
    11281 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
    11341 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
    11401 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
    11461 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
    11521 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
    11581 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
    11641 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
    11701 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaaccat
    11761 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    11821 caaaggatct tc
//
