LOCUS       sequence-345628-       11477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSOUP helper plasmid needed for Agrobacterium-mediated plant 
            transformation - Expression of GOXL genes tagged with Citrine under 
            RUBY promoter for transgene complementation of ruby-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Sola K, Dean GH, Li Y, Lohmann J, Movahedan M, Gilchrist EJ, Adams 
            KL, Haughn GW
  TITLE     Expression Patterns and Functional Characterisation of Arabidopsis 
            GALACTOSE OXIDASE-LIKE (GOXL) Genes Suggest Specialised Roles for 
            Galactose Oxidases in Plants.
  JOURNAL   Plant Cell Physiol. 2021 May 27. pii: 6286013. doi: 
            10.1093/pcp/pcab073.
  PUBMED    34042158
REFERENCE   2  (bases 1 to 11477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2021 May 27. pii: 6286013. doi: 10.1093/pcp/pcab073."
COMMENT     Sequence Label: pGreenII 0229/ProRUBY:GOXL2-Citrine:NOSt
FEATURES             Location/Qualifiers
     source          1..11477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    656..678
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     terminator      complement(702..954)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(974..1525)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1567..1746)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1978..2000
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1992..2009
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1993..2009
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2019..2038
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2019..2037
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8204..8220)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8204..8220)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             join(8243..8245,8246..8956)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8285..8306)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8546..8565)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8893..8914
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      8998..9250
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(9269..9289)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9269..9287)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9308..9324)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9308..9324)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9321..9343)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9332..9348
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9356..9362,9363..9380,9381..9386))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9401..9422
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9539..9556)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9661..9685
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      9836..10271
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     primer_bind     complement(10551..10570)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             10562..11377
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             10562..11377
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(10635..10654)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatga gatcttggca
      661 ggatatattg tggtgtaacg ttatcagctt gcatgccggt cgatctagta acatagatga
      721 caccgcgcgc gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta
      781 aatgtataat tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta
      841 catgttaatt attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag
      901 accggcaaca ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatctgcttg
      961 actctagggg tcatcagatt tcggtgacgg gcaggaccgg acggggcggc accggcaggc
     1021 tgaagtccag ctgccagaaa cccacgtcat gccagttccc gtgcttgaag ccggccgccc
     1081 gcagcatgcc gcggggggca tatccgagcg cctcgtgcat gcgcacgctc gggtcgttgg
     1141 gcagcccgat gacagcgacc acgctcttga agccctgtgc ctccagggac ttcagcaggt
     1201 gggtgtagag cgtggagccc agtcccgtcc gctggtggcg gggggagacg tacacggtcg
     1261 actcggccgt ccagtcgtag gcgttgcgtg ccttccaggg acccgcgtag gcgatgccgg
     1321 cgacctcgcc gtccacctcg gcgacgagcc agggatagcg ctcccgcaga cggacgaggt
     1381 cgtccgtcca ctcctgcggt tcctgcggct cggtacggaa gttgaccgtg cttgtctcga
     1441 tgtagtggtt gacgatggtg cagaccgccg gcatgtccgc ctcggtggca cggcggatgt
     1501 cggccgggcg tcgttctggg ctcatggtag atcccctcga tcgagttgag agtgaatatg
     1561 agactctaat tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga
     1621 aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga
     1681 cgtatgtgct tagctcatta aactccagaa acccgcggct cagtggctcc ttcaacgttg
     1741 cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac
     1801 gtgactccct taattctccg ctcatgatcg ataacattaa cgcttacaat ttccattcgc
     1861 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     1921 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     1981 agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg
     2041 aattgggtac cgctaaccca atctcaatcg aaccggacca aattagaatg aaatggtttt
     2101 agtgagttac atggttctta atttgactgg atgaaattaa ttaaaggcac tatatagatg
     2161 aaactaaaac tacaagaaca ttgctctaac ttttacacaa ctttaaaaaa aaaaaaaaaa
     2221 aaaatcgtgg aatgttgatt catttgacct ttattctgga ttgaaagtga tgcaaaaatg
     2281 actttatcta ataaataaaa aaaagaagac tttatctgat gtagtgaaat tcttgtattt
     2341 gttgatgcaa tgatgctatg gattgttttc aaatgtttca attacgtatc aaattatttt
     2401 atttgtacca acacatgcaa gtatgcaacc atggcctcgt cgtaaagaac tttgggcaac
     2461 atacaaattt gggcaaacat ctataacaca ttggattttt ttgatcgatt taatttgatt
     2521 cttggaatta ctattacctt cttcttttaa aaattcagta agatatgtgt taccgtgtga
     2581 aagtctatat gtgtgggaac gaaaggtttg aagacgagta agaaagtaga agagcacaat
     2641 gcgaaacttt attggtatac gtaactgttt attgacgaca tttgtaggaa caaaaattgc
     2701 aagataacac attttttagt gtttgctttg cttgtttata gtaaccggta ggcagtggcg
     2761 gaggcagaat aattagatac aggggtcact ctttttgtaa gccaaatacc aaaaaaaaaa
     2821 aattaagaaa aatgataata tcctcataaa tgataatttc aaaaaaaaat atatatatga
     2881 gaaagaaaaa aactgttcta gtaaagtaaa cattaaatct aaagtaattt atactgtgtt
     2941 aaacaataat aatgagatta acttagttta atagacttat ttttttattt ataaaattaa
     3001 ttgggttaat ttgttgtagg tttaatattt tttgttagac tatatttgaa tatatattga
     3061 tgggggtcaa taatattttg ggaaagggtc aatttataaa aattaacaaa acctaaagta
     3121 attttctaaa atccataggg gtcaattgac ccccatgatt atacactccc tccgccactg
     3181 ccggtagggt ttgatgtctt ggattgttaa gttttaaagt tttgtctcat gatctatgta
     3241 agtgcttaag ccggcgctag cataaattga acctttaatc agaatgtagt agttgacctg
     3301 gatggatgac acttttatgc gagatgggtc gagcgacgct aaccattagg ccctcgatcg
     3361 gcagtcggca gatataactt agatacagtg gatagtgaca tatcctctta ataaatatgt
     3421 tttaaatgtt agattaactt atgacctaaa gaaaagaagt tagattaaca ttttttagaa
     3481 attattattt cggataggtt taataagcat aagtcagcga aacttataag gtatttattt
     3541 acaattataa aatcaacagc ttccaaacaa aatctttttt ctttttgact tcttaaaaca
     3601 aaatctcata ctaaccgatt taacattatg tttgtgaata atatggtaac gcgaatgatt
     3661 agcaacataa aaaacaaaga gtcaactaac cgcattcatg aatggaatgg aatgtcacgg
     3721 tcaatcatta gatttggtgt tacggaatac atatttatta aatgtatatg aagaaaaaag
     3781 catggccaat aataatgtaa ataaattggc tcaacatgcg atgtatgtac ataatttaga
     3841 gtggaaaatg aaaagatata tacaatgtgt attaatatag atgtacgcag ttgtataaaa
     3901 ttgatggaca tgatttaggt ttttttttaa aaaattgatg gacatttttc aaatatcaaa
     3961 atgtttaatt tatattttct acatataatt gaaattaaac aaaggagata taagttctac
     4021 ataattttac tttgtatcgt tggaacaaac acgtacagta gtattatttt atatatgtgc
     4081 atgtatgttt tgcgttcctt taaaaatcaa atacatggga agataacatg caacgaacca
     4141 gatgatacag cgcctgatga gaggtcaaaa acgtaacgag tttgacttgt ataatattaa
     4201 tatgtagttg catgtttgat gttgacacag catcgccatc attaattcgt tctctgcagt
     4261 ctaccgaact aattaatctc aaataatcga tgagtacata tatgttaagt ccgtcgaggc
     4321 taatgtataa ttcaataata taaatatgtt cattcgagga tatagttcat attattatat
     4381 atctgtttaa aattattgac tccaatcatc ctataaacta agagggttga caccgcagca
     4441 aagaaaaaaa taaaatatac cataattcat atatttgcac aatctgaatt ataatggaaa
     4501 ttaagtttgc tcaaacaaaa aaaaagggtt ggctccatat acgaaattgt taatttgtaa
     4561 ttttgtagga cttctaatta aaaaaatata tatcaataaa tgattatttt atatatgtca
     4621 attatatctg atcattaatt aattaattgt ttcgaaaata attaattaat tttgaaattt
     4681 aaattaaagc aatattaaat gtaaaaattt taaattatat taaactagaa atttattgtg
     4741 agcatttttg tgtatagatc aagatatgat gaatcttgtg ggattttttt tattctataa
     4801 caggtgttgt ttcatatgct acgcattttt atatatttaa ccaaacataa tgacaagttg
     4861 acaacgacta gtatttcttc ccatttagat gataagatta gtccaaatac tccaaaccgt
     4921 ctgttggatt aagaatgacc ggaatacgat tactccaaaa cgcatatggg tttactggtc
     4981 gagaatatac ctttggatta agatcatgta gagttatttc attacgattt tgatttcttt
     5041 tggttattgt gaatatgttt tttactttct tttttctgtt agagatcatt tgtgaatatg
     5101 ttaagtggta agtgtacaat ttttcatatt ttgaatcgag tatcataaat atcattgtac
     5161 acataccagt ttatttattt cgtcctgata ttaacaagta acaacgtcaa ttttgagatc
     5221 aattatttga aaatatagaa aactgaaagt attaaccaat ttagtttgat tctaattaga
     5281 gtcatcgtat tgtgctagta atcggtgacg ggaaccacat aaaacgacag tttatcaaca
     5341 taaaaaaaaa tcacaaacac atagaagact ctagttttgt tataaacttg tgctaaataa
     5401 ttaacacgat acataatact cttcattatt atatatactt ttcatgtata ataatgcatg
     5461 gacattacac aaatggaatg aatagtacaa ttgtatgtgc atgaatgttt ttctaaaaac
     5521 tgacatccgt ttgtatgcta ttttatcctt gtttgttatt taatagtaga aaacttgcga
     5581 ggaccaaaat aattagcatg caaagaacca gaagaaatcg tgagaactaa aacgtaaaga
     5641 actcttgtga cttgtataat atatagtttg tatgcatgtt tgatgttgac acaacatatc
     5701 tccatcatta attcgtccct tgcagtctct caaacttatt aatctcaaat aatcgatgtt
     5761 tatagattaa gtccctcgag actaataatg taaatccaat gatacaatgt ggttccttcg
     5821 acgacgaatc atattctgat ttctgtatat ctgtaatata aagtacgatt attattatat
     5881 ttgaatacaa tcatacttaa taaaatattg tgtcatatat atttatctcc ataaatgttt
     5941 ctcttgactg aaatgtgaat acccaaacat aaaaacatat acagtaaata ctttgttcaa
     6001 ttcccattaa atttctcttt gaatctaaaa tcagacatag aaatactgtt gtttagagaa
     6061 agattttgaa tatatagaac attaaatcgc cgcagaactt tgttgtttag agaaagattt
     6121 taaatatgat aagaactcaa ctaaagaaaa agaaaaaaaa ttatgacgtc tcaatttaga
     6181 gttttattgg ttcttttgta cagttctgta atgttaggaa tcgttttata cacaaaggta
     6241 acttaaatct ttcaaacgtc tacggtagtg gacggtcatt aaaatcagag ttaagaaaac
     6301 gaacaaacca aatttaatca tatcttatac actcgagacc ttgattatat attactcttt
     6361 tatatattaa cagaactaga aataaaaact atacattatt agaatatcgt aacgtatact
     6421 aatatcccat cgaccagtat ataaacgaca caagtcacag tacattctca tcacatacca
     6481 caaaatatct cataaagatc aaataaacac taaacctgaa agcaaaaaag ggcccatggc
     6541 agaacttatt ccttggtact acacttcagg gtcagggatc gtacttgtac ttcaaaccat
     6601 ttttctcttc tcgatcgtac gtgctgattt accaggctca tgggaactca ttgtacaaga
     6661 cgccggtata gcttctatgc acacggcggt gacccggttc aacacggtca tccttctaga
     6721 ccgaactaac atcggaccgt cgagaaaggc tcttgaccgt caccggtgcc ggagagaccc
     6781 aaaagacgcc gctttgaaac gcgattgtta cgctcactcg gttctgttcg accttggtac
     6841 aaatcagatc agaccactga tgatacaaac cgacacgtgg tgctcgtcgg gccagttttt
     6901 atccgacgga tcgttgctac aaacgggtgg ggataaagac gggtttaaaa agatacgtaa
     6961 attcgaaccc tgtgacccga acgaaacgtg cgattgggtg gagcttcaag ataccgaatt
     7021 gataaccgga cgctggtacg cttctaacca gatattaccg gatggttcgg ttatcatcgt
     7081 cggtggtaga ggaactaata cggttgagta ttaccctcca cgcgaaaacg gtgccgttcc
     7141 gtttcagttt cttgctgacg tggaagacaa acaaatggat aatctctatc cgtacgttca
     7201 tctcctcccc gacgacgacg gtggcaatct cttcatcttc gccaacagtc gcgccgttaa
     7261 atacgatcac cggataaacg ccgtcgtgaa agagtatcca ccgttagacg gtggtccgag
     7321 gaattatccg tcaggtggat catcggcgat gctagctatc caaggagatt tcaccacagc
     7381 ggagattcta atctgcggcg gagcacaatc cggagccttc acagcgcgtg caattgatgc
     7441 tccggcgcat ggaacctgcg gacgaatcgt agctaccgcg gcggatcctg tttgggtgac
     7501 ggaggagatg ccgttcggga gaatcatggg agatatggtg aatttaccga cgggagagat
     7561 tctgatcata aacggagctc aagccggaag ccaaggattc gaaatgggat ccgatccgtg
     7621 tttatacccg cttttatacc gacccgatca accaattggt ttacgattca tgacgttgaa
     7681 cccaggaact gtaccgagaa tgtaccattc cacggcgaat cttttaccgg acggtagaat
     7741 tcttctcgcc ggaagcaatc ctcactattt ctacaaattc aacgccgaat tcccaacgga
     7801 gctacggatc gaagcgtttt caccggagta tctgtcaccg gatcgagcta atctccgacc
     7861 agagatccaa gaaatcccgc agatcatacg gtacggagag gttttcgacg tgttcgtgac
     7921 cgtgccgttg ccggtggtgg ggataattca gatgaattgg ggaagtgcgc cttttgcgac
     7981 tcattcgttt tctcaaggtc aacggctcgt taagttgacc gttgctccgt ctgtgcctga
     8041 cggagtaggt cgttatagga ttcagtgtac ggctccacct aacggcgccg tttctccgcc
     8101 tgggtattat atggccttcg ctgttaacca gggagttcct agcattgctc gatggatacg
     8161 aatagtttct ggaagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga
     8221 gcctggaggt ggaggtggag ctgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     8281 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     8341 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     8401 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg
     8461 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     8521 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     8581 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     8641 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     8701 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     8761 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     8821 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     8881 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     8941 ggacgagctg tacaaggatc ctgctggtgc tgctgcgcct ctagcataag cggccgcgat
     9001 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     9061 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     9121 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     9181 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     9241 ttactagatc gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     9301 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     9361 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     9421 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     9481 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     9541 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9601 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgaaggcct
     9661 tgacaggata tattggcggg taaactaagt cgctgtatgt gtttgtttga gatctaattc
     9721 cggggatcgg aaatccagaa gcccgagagg ttgccgcctt tcgggctttt tctttttcaa
     9781 aaaaaaaaat ttataaaacg atctgttgcg gccggccgcc gggttgtggg caaaggcgct
     9841 cgacggtggg caaccgcttg cggttgtcca cgggcggagc cggtgcgcgt agcgcattgt
     9901 ccacaagcca agggcgacca ataattgata tatatattca taattgaaaa gctaattgaa
     9961 catactactt gctgtaacta cttgccggag cgaggggtgt ttgcaagctg ttgatctgaa
    10021 agggctatta gcgttctcac gtgccttttt gattagcgat ttcacgtgac cttattagcg
    10081 atttcacgta ctccgattag cgatttcacg taccctgatt agcgatttca cgtggatagt
    10141 ttttggagcg ggccggaaag ccccgtgaat caaggctttg cggggcatta gcggtttcac
    10201 gtggataact accctctatc cacaggcttc cggggataaa aaagcccgct cgacggcggg
    10261 ctgttggatg gggatcgcct gaatcgcccc atcatccagc cagaaagtga gggagccacg
    10321 gttgatgaga gctttgttgt aggtggacca gttggtgatt ttgaactttt gctttgccac
    10381 ggaacggtct gcgttgtcgg gaagatgcgt gatctgatcc ttcaactcag caaaagttcg
    10441 atttattcaa caaagccacg ttgtgtctca aaatctctga tgttacattg cacaagataa
    10501 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
    10561 tatgagccat attcaacggg aaacgtcttg ctcaaggccg cgattaaatt ccaacatgga
    10621 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
    10681 ctaccgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
    10741 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
    10801 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
    10861 gatcccaggg aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
    10921 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
    10981 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
    11041 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
    11101 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
    11161 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
    11221 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
    11281 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
    11341 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
    11401 accatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    11461 aagatcaaag gatcttc
//
