LOCUS       sequence-345634-        6235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad-Host range expression vector, pBBR origin, LuxR activator, 
            PLuxB promoter, mRFP, Gentamicin Resistance Marker. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Schuster LA, Reisch CR
  TITLE     A plasmid toolbox for controlled gene expression across the 
            Proteobacteria.
  JOURNAL   Nucleic Acids Res. 2021 Jun 14. pii: 6298615. doi: 
            10.1093/nar/gkab496.
  PUBMED    34125913
REFERENCE   2  (bases 1 to 6235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jun 14. pii: 6298615. doi: 10.1093/nar/gkab496."
COMMENT     Sequence Label: pBLxR5
FEATURES             Location/Qualifiers
     source          1..6235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1055..1826
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1827..2489
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     3183..3205
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3197..3214
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3198..3214
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        4192..4241
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             4286..4297
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             4304..4981
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     promoter        join(5258..5263,5264..5280,5281..5286)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5475..6008
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gtccagaacg ggcttcaggc gctcccgaag gtctcgggcc gtctcttggg cttgatcggc
       61 cttcttgcgc atctcacgcg ctcatgcggc ggcctgtagg gcaggctcat acccctgccg
      121 aaccgctttt gtcagccggt cggccacggc ttccggcgtc tcaacgcgct ttgagattcc
      181 cagcttttcg gccaatccct gcggtgcata ggcgcgtggc tcgaccgctt gcgggctgat
      241 ggtgacgtgg cccactggtg gccgctccag ggcctcgtag aacgcctgaa tgcgcgtgtg
      301 acgtgccttg ctgccctcga tgccccgttg cagccctaga tcggccacag cggccgcaaa
      361 cgtggtctgg tcgcgggtca tctgcgcttt gttgccgatg aactccttgg ccgacagcct
      421 gccgtcctgc gtcagcggca ccacgaacgc ggtcatgtgc gggctggttt cgtcacggtg
      481 gatgctggcc gtcacgatgc gatccgcccc gtacttgtcc gccagccact tgtgcgcctt
      541 ctcgaagaac gccgcctgct gttcttggct ggccgacttc caccattccg ggctggccgt
      601 catgacgtac tcgaccgcca acacagcgtc cttgcgccgc ttctctggca gcaactcgcg
      661 cagtcggccc atcgcttcat cggtgctgct ggccgcacag tgctcgttct ctggcgtcct
      721 gctggcgtca gcgttgggcg tctcgcgctc gcggtaggcg tgcttgagac tggccgccac
      781 gttgcccatt ttcgccagct tcttgcatcg catgatcgcg tatgccgcca tgcctgcccc
      841 tcccttttgg tgtccaaccg gctcgacggg ggcagcgcaa ggcggtgcct ccggcgggcc
      901 actcaatgct tgagtatact cactagactt tgcttcgcaa agtcgtgacc gcctacggcg
      961 gctgcggcgc cctacgggct tgctctccgg gcttcgccct gcgcggtcgc tgcgctccct
     1021 tgccagcccg tggatatgtg gacgatggcc gcgagcggcc accggctggc tcgcttcgct
     1081 cggcccgtgg acaaccctgc tggacaagct gatggacagg ctgcgcctgc ccacgagctt
     1141 gaccacaggg attgcccacc ggctacccag ccttcgacca catacccacc ggctccaact
     1201 gcgcggcctg cggccttgcc ccatcaattt ttttaatttt ctctggggaa aagcctccgg
     1261 cctgcggcct gcgcgcttcg cttgccggtt ggacaccaag tggaaggcgg gtcaaggctc
     1321 gcgcagcgac cgcgcagcgg cttggccttg acgcgcctgg aacgacccaa gcctatgcga
     1381 gtgggggcag tcgaagggcg aagcccgccc gcctgccccc cgagcctcac ggcggcgagt
     1441 gcgggggttc caagggggca gcgccacctt gggcaaggcc gaaggccgcg cagtcgatca
     1501 acaagccccg gaggggccac tttttgccgg agggggagcc gcgccgaagg cgtgggggaa
     1561 ccccgcaggg gtgcccttct ttgggcacca aagaactaga tatagggcga aatgcgaaag
     1621 acttaaaaat caacaactta aaaaaggggg gtacgcaaca gctcattgcg gcaccccccg
     1681 caatagctca ttgcgtaggt taaagaaaat ctgtaattga ctgccacttt tacgcaacgc
     1741 ataattgttg tcgcgctgcc gaaaagttgc agctgattgc gcatggtgcc gcaaccgtgc
     1801 ggcaccccta ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag
     1861 ccaagaacaa gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg
     1921 ggcttattgc gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg
     1981 gccaccagaa cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt
     2041 tgcggacggt ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga
     2101 agctcaacgg ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc
     2161 agccccgcga ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg
     2221 accaggacga atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg
     2281 gcgagcagca actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg
     2341 gcatggaacc agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc
     2401 agcgcctgcc gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc
     2461 cgacacgggt cacgctgccg cgccggtagc acttgggttg cgcagcaacc cgtaagtgcg
     2521 ctgttccaga ctatcggctg tagccgcctc gccgccctat accttgtctg cctccccgcg
     2581 ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg
     2641 ctaacggatt caccgttttt atcaggctct gggaggcaga ataaatgatc atatcgtcaa
     2701 ttattacctc cacggggaga gcctgagcaa actggcctca ggcatttgag aagcacacgg
     2761 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
     2821 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat
     2881 ccgcttatta tcacttattc aggcgtagca accaggcgtt taagggcacc aataactgcc
     2941 ttaaaaaaat tacgccccgc cctgccactc atcgcagtac ggcctattgg ttaaaaaatg
     3001 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcca
     3061 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     3121 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     3181 ttcccagtca cgacgttgta aaacgacggc cagtgatcaa tttttaaagt atgggcaatc
     3241 aattgctcct gttaaaattg ctttagaaat actttggcag cggtttgttg tattgagttt
     3301 catttgcgca ttggttaaat ggaaagtgac agtacgctca ctgcaaccta atatttttga
     3361 aatatcccaa gagctttttc cttcgcatgc ccacgctaaa cattcttttt ctcttttggt
     3421 taaatcgttg tttgatttat tatttgctat atttattttt cgataattat caactagaga
     3481 aggaacaatt aatggtatgt tcatacacgc atgtaaaaat aaactatcta tatagttgtc
     3541 tttttctgaa tgtgcaaaac taagcattcc gaagccattg ttagccgtat gaatagggaa
     3601 actaaaccca gtgataagac ctgatgtttt cgcttcttta attacatttg gagatttttt
     3661 atttacagca ttgttttcaa atatattcca attaattggt gaatgattgg agttagaata
     3721 atctactata ggatcatatt ttatcaaatt agcgtcatca taatattgcc tccatttttt
     3781 agggtaatta tctaggattg aaatatcaga tttaaccata gaatgaggat aaatgatcgc
     3841 gagtaaataa tattcacaat gtaccatttt agtcatatca gataagcatt gattaatatc
     3901 attattgctt ctacaagctt taattttatt aattattctg tatgtgtcgt cggcatttat
     3961 gtttttcatt ctgcccctcc gaaaggctta agtcctcgcg tagtacctgg gcgctatcat
     4021 gccataccgc gaaaggtttt gcgccattcg atggcgcgcc gcccaattat tgaaggcctc
     4081 cctaacgggg ggcctttttt tgtttctggt ctcccgctta acgatcgttg gctgacctgt
     4141 aggatcgtac aggtttacgc aagaaaatgg tttgttacag tcgaataaaa gctgtcaccg
     4201 gatgtgcttt ccggtctgat gagtccgtga ggacgaaaca gcctctacaa ataattttgt
     4261 ttaagaactg ggaagagcgc agattaaaga ggagaaatac tagatggctt cctccgaaga
     4321 cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga
     4381 gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact
     4441 gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca
     4501 gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc
     4561 cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac
     4621 cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg
     4681 taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc
     4741 caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa
     4801 actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc
     4861 ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga
     4921 agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta
     4981 ataacgctga tagtgctagt gtagatcgcg ctttccagtc gggaaacctg tcgtgccagc
     5041 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgcatgcata
     5101 aaaactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     5161 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     5221 acgcacaccg tggaaacgga tgaaggcacg aacccagttg acataagcct gttcggttcg
     5281 taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
     5341 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca
     5401 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     5461 atggagcagc aacgatgtta cgcagcagca acgatgttac gcagcagggc agtcgcccta
     5521 aaacaaagtt aggtggctca agtatgggca tcattcgcac atgtaggctc ggccctgacc
     5581 aagtcaaatc catgcgggct gctcttgatc ttttcggtcg tgagttcgga gacgtagcca
     5641 cctactccca acatcagccg gactccgatt acctcgggaa cttgctccgt agtaagacat
     5701 tcatcgcgct tgctgccttc gaccaagaag cggttgttgg cgctctcgcg gcttacgttc
     5761 tgcccaagtt tgagcagccg cgtagtgaga tctatatcta tgatctcgca gtctccggag
     5821 agcaccggag gcagggcatt gccaccgcgc tcatcaatct cctcaagcat gaggccaacg
     5881 cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg tgacgatccc gcagtggctc
     5941 tctatacaaa gttgggcata cgggaagaag tgatgcactt tgatatcgac ccaagtaccg
     6001 ccacctaaca attcgttcaa gccgagatcg gcttcccggc cgcggagttg ttcggtaaat
     6061 tgtcacaacg ccgcaggtgg cacttttcgg ggaaatgtgc gcgcccgcgt tcctgctggc
     6121 gctgggcctg tttctggcgc tggacttccc gctgttccgt cagcagcttt tcgcccacgg
     6181 ccttgatgat cgcggcggcc ttggcctgca tatcccgatt caacggcccc agggc
//
