LOCUS       sequence-345644-        9933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UAS vector to express sfcherry2(1-10) in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9933)
  AUTHORS   Kamiyama R, Banzai K, Liu P, Marar A, Tamura R, Jiang F, Fitch MA, 
            Xie J, Kamiyama D
  TITLE     Cell-type-specific, multicolor labeling of endogenous proteins with 
            split fluorescent protein tags in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jun 8;118(23). pii: 2024690118. doi: 
            10.1073/pnas.2024690118.
  PUBMED    34074768
REFERENCE   2  (bases 1 to 9933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-06-8"; volume: "118(23). pii: 
            2024690118. doi"; pages: " 10.1073/pnas.2024690118"
COMMENT     Sequence Label: pACUH-sfcherry2(1-10)
FEATURES             Location/Qualifiers
     source          1..9933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(216..804)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(296..315)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(975..1766,1767..1835))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1598..1617
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1836..1940)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2008..2026
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2064..2086)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2186..2205
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2415..2484
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(3028..3058,3059..3260)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3341..3435
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3459..3553
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3572..3810
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3757..3778
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     complement(3983..4001)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     intron          4560..4625
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4755..4775
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5047..5181
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5084..5103)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5138..5157
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5208..5226)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5678..5749,6151..6424,6499..7153,7215..7530,7734..7865,
                     7936..8550)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9329..9883,9884..9914)
                     /label=P element 5' end
ORIGIN
        1 agcggcagat cggcggcgga gaagttaagc gtctccagga tgaccttgcc cgaactgggg
       61 cacgtggtgt tcgacgatgt gcagctaatt tcgcccggct ccacgtccgc ccattggtta
      121 atcagcagac cctcgttggc gtaacggaac catgagaggt acgacaacca tttgaggtat
      181 actggcaccg agcccgagtt caagaagaag gcgtttttcc ataggctccg cccccctgac
      241 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      301 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      361 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      421 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      481 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      541 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      601 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
      661 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      721 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      781 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      841 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
      901 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
      961 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1021 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1081 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1141 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1201 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1261 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1321 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1381 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1441 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1501 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1561 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1621 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1681 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1741 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1801 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1861 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1921 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     1981 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     2041 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     2101 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     2161 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     2221 atcgacatgc ccgccgtgac cgtcgagaac ccgctgacgc tgccccgcgt atccgcaccc
     2281 gccgacgccg tcgcacgtcc cgtgctcacc gtgaccaccg cgcccagcgg tttcgagggc
     2341 gagggcttcc cggtgcgccg cgcgttcgcc gggatcaact accgccacct cgacccgttc
     2401 atcatgatgg accagatggg tgaggtggag tacgcgcccg gggagcccaa gggcacgccc
     2461 tggcacccgc accgcggctt cgagaccgtg acctacatcg tcgatatgcg gtgtgaaata
     2521 ccgcaccgaa tcgcgcggaa ctaacgacag tcgctccaag gtcgtcgaac aaaaggtgaa
     2581 tgtgttgcgg agagcgggtg ggagacagcg aaagagcaac tacgaaacgt ggtgtggtgg
     2641 aggtgaatta tgaagagggc gcgcgatttg aaaagtatgt atataaaaaa tatatcccgg
     2701 tgttttatgt agcgataaac gagtttttga tgtaaggtat gcaggtgtgt aagtcttttg
     2761 gttagaagac aaatccaaag tctacttgtg gggatgttcg aaggggaaat acttgtattc
     2821 tataggtcat atcttgtttt tattggcaca aatataatta cattagcttt ttgagggggc
     2881 aataaacagt aaacacgatg gtaataatgg taaaaaaaaa aaacaagcag ttatttcgga
     2941 tatatgtcgg ctactccttg cgtcgggccc gaagtcttag agccagatat gcgagcaccc
     3001 ggaagctcac gatgagaatg gccagaccat gatgaaataa cataaggtgg tcccgtcggc
     3061 aagagacatc cacttaacgt atgcttgcaa taagtgcgag tgaaaggaat agtattctga
     3121 gtgtcgtatt gagtctgagt gagacagcga tatgattgtt gattaaccct tagcatgtcc
     3181 gtggggtttg aattaactca taatattaat tagacgaaat tatttttaaa gttttatttt
     3241 taataatttg cgagtacgca aagcttggct gcatccaacg cgttgggagc tctccggatc
     3301 caagctggcc gcggctcgat ccgcttgcat gcctgcaggt cggagtactg tcctccgagc
     3361 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     3421 gtactgtcct ccgagcggaa gcttgcatgc ctgcaggtcg gagtactgtc ctccgagcgg
     3481 agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt
     3541 actgtcctcc gagcggagac tctagcgagc gccggagtat aaatagaggc gcttcgtcta
     3601 cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca
     3661 aataaacaag cgcagctgaa caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa
     3721 tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa
     3781 gtaattattg aatacaagaa gagaactctg aatagggaat tgggaattcg ttaacagatc
     3841 tgcggccgca tggaggagga caacatggcc atcatcaagg agttcatgag attcaaggtg
     3901 cacatggagg gcagcgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccac
     3961 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
     4021 gcctgggaca tcctgagccc ccagttcatg tacggcagca aggcctacgt gaagcacccc
     4081 gccgacatcc ccgactacct gaagctgagc ttccccgagg gcttcacctg ggagagagtg
     4141 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg acagcagcct gcaggacggc
     4201 cagttcatct acaaggtgaa gctgctgggc atcaacttcc ccagcgacgg ccccgtgatg
     4261 cagaagaaga ccatgggctg ggaggccagc accgagagaa tgtaccccga ggacggcgcc
     4321 ctgaagggcg agatcaacca gagactgaag ctgaaggacg gcggccacta cgacgccgag
     4381 gtgaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtggac
     4441 atcaagctgg acatcaccag ccacaacgag gactgatcta gaggatcttt gtgaaggaac
     4501 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     4561 taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     4621 tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     4681 aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     4741 aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     4801 aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     4861 tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatatttga
     4921 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     4981 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     5041 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5101 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5161 gtatcttatc atgtctggat ccactagaag gcctaattct agtatgtatg taagttaata
     5221 aaacccattt ttgcggaaag tagataaaaa aaacattttt tttttttact gcactggata
     5281 tcattgaact tatctgatca gttttaaatt tacttcgatc caagggtatt tgatgtacca
     5341 ggttctttcg attacctctc actcaaaatg acattccact caaagtcagc gctgtttgcc
     5401 tccttctctg tccacagaaa tatcgccgtc tctttcgccg ctgcgtccgc tatctctttc
     5461 gccaccgttt gtagcgttac gtagcgtcaa tgtccgcctt cagttgcatt ttgtcagcgg
     5521 tttcgtgacg aagctccaag cggtttacgc catcaattaa acacaaagtg ctgtgccaaa
     5581 actcctctcg cttcttattt ttgtttgttt tttgagtgat tggggtggtg attggttttg
     5641 ggtgggtaag caggggaaag tgtgaaaaat cccggcaatg ggccaagagg atcaggagct
     5701 attaattcgc ggaggcagca aacacccatc tgccgagcat ctgaacaatg tgagtagtac
     5761 atgtgcatac atcttaagtt cacttgatct ataggaactg cgattgcaac atcaaattgt
     5821 ctgcggcgtg agaactgcga cccacaaaaa tcccaaaccg caattgcaca aacaaatagt
     5881 gacacgaaac agattattct ggtagctgtt ctcgctatat aagacaattt ttgagatcat
     5941 atcatgatca agacatctaa aggcattcat tttcgactat attctttttt acaaaaaata
     6001 taacaaccag atattttaag ctgatcctag atgcacaaaa aataaataaa agtataaacc
     6061 tacttcgtag gatacttcgg ggtacttttt gttcggggtt agatgagcat aacgcttgta
     6121 gttgatattt gagatcccct atcattgcag ggtgacagcg gagcggcttc gcagagctgc
     6181 attaaccagg gcttcgggca ggccaaaaac tacggcacgc tccggccacc cagtccgccg
     6241 gaggactccg gttcagggag cggccaacta gccgagaacc tcacctatgc ctggcacaat
     6301 atggacatct ttggggcggt caatcagccg ggctccggat ggcggcagct ggtcaaccgg
     6361 acacgcggac tattctgcaa cgagcgacac ataccggcgc ccaggaaaca tttgctcaag
     6421 aacggtgagt ttctattcgc agtcggctga tctgtgtgaa atcttaataa agggtccaat
     6481 taccaatttg aaactcagtt tgcggcgtgg cctatccggg cgaacttttg gccgtgatgg
     6541 gcagttccgg tgccggaaag acgaccctgc tgaatgccct tgcctttcga tcgccgcagg
     6601 gcatccaagt atcgccatcc gggatgcgac tgctcaatgg ccaacctgtg gacgccaagg
     6661 agatgcaggc caggtgcgcc tatgtccagc aggatgacct ctttatcggc tccctaacgg
     6721 ccagggaaca cctgattttc caagccatgg tgcggatgcc acgacatctg acctatcggc
     6781 agcgagtggc ccgcgtggat caggtgatcc aggagctttc gctcagcaaa tgtcagcaca
     6841 cgatcatcgg tgtgcccggc agggtgaaag gtctgtccgg cggagaaagg aagcgtctgg
     6901 cattcgcctc cgaggctcta accgatccgc cgcttctgat ctgcgatgag cccacctccg
     6961 gactggactc ctttaccgcc cacagcgtcg tccaggtgct gaagaagctg tcgcagaagg
     7021 gcaagaccgt catcctgacc attcatcagc cgtcttccga gctgtttgag ctctttgaca
     7081 agatccttct gatggccgag ggcagggtag ctttcttggg cactcccagc gaagccgtcg
     7141 acttcttttc ctagtgagtt cgatgtgttt attaagggta tctagtatta cataacatct
     7201 caactcctat ccagcgtggg tgcccagtgt cctaccaact acaatccggc ggacttttac
     7261 gtacaggtgt tggccgttgt gcccggacgg gagatcgagt cccgtgatcg gatcgccaag
     7321 atatgcgaca attttgccat tagcaaagta gcccgggata tggagcagtt gttggccacc
     7381 aaaaatctgg agaagccact ggagcagccg gagaatgggt acacctacaa ggccacctgg
     7441 ttcatgcagt tccgggcggt cctgtggcga tcctggctgt cggtgctcaa ggaaccactc
     7501 ctcgtaaaag tgcgacttat tcagacaacg gtgagtggtt ccagtggaaa caaatgatat
     7561 aacgcttaca attcttggaa acaaattcgc tagattttag atagaattgc ctgattccac
     7621 acccttctta gtttttttca atgagatgta tagtttatag ttttgcagaa gataaataaa
     7681 tttcatttaa ctcgcgaata ttaatgagat gcgagtaaca ttttaatttg cagatggttg
     7741 ccatcttgat tggcctcatc tttttgggcc aacaactcac gcaagtgggt gtgatgaata
     7801 tcaacggagc catcttcctc ttcctgacca acatgacctt tcaaaacgtc tttgccacga
     7861 taaatgtaag tcatgtttag aatacatttg catttcaata atttactaac tttctaatga
     7921 atcgattcga tttaggtgtt cacctcagag ctgccagttt ttatgaggga ggcccgaagt
     7981 cgactttatc gctgtgacac atactttctg ggcaaaacga ttgccgaatt gccgcttttt
     8041 ctcacagtgc cactggtctt cacggcgatt gcctatccga tgatcggact gcgggccgga
     8101 gtgctgcact tcttcaactg cctggcgctg gtcactctgg tggccaatgt gtcaacgtcc
     8161 ttcggatatc taatatcctg cgccagctcc tcgacctcga tggcgctgtc tgtgggtccg
     8221 ccggttatca taccattcct gctctttggc ggcttcttct tgaactcggg ctcggtgcca
     8281 gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga gggtctgctg
     8341 attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc gaacaccacg
     8401 tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc cgatctgccg
     8461 ctggactacg tgggtctggc cattctcatc gtgagcttcc gggtgctcgc atatctggct
     8521 ctaagacttc gggcccgacg caaggagtag ccgacatata tccgaaataa ctgcttgttt
     8581 ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa aaagctaatg
     8641 taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt atttcccctt
     8701 cgaacatccc cacaagtaga ctttggattt gtcttctaac caaaagactt acacacctgc
     8761 ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata ttttttatat
     8821 acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc acgtttcgta
     8881 gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt gttcgacgac
     8941 cttggagcga ctgtcgttag ttccgcgcga ttcggttcgc tcaaatggtt ccgagtggtt
     9001 catttcgtct caatagaaat tagtaataaa tatttgtatg tacaatttat ttgctccaat
     9061 atatttgtat atatttccct cacagctata tttattctaa tttaatatta tgacttttta
     9121 aggtaatttt ttgtgacctg ttcggagtga ttagcgttac aatttgaact gaaagtgaca
     9181 tccagtgttt gttccttgtg tagatgcatc tcaaaaaaat ggtgggcata atagtgttgt
     9241 ttatatatat caaaaataac aactataata ataagaatac atttaattta gaaaatgctt
     9301 ggatttcact ggaactagaa ttaattcggc tgctgctcta aacgacgcat ttcgtactcc
     9361 aaagtacgaa ttttttccct caagctctta ttttcattaa acaatgaaca ggacctaacg
     9421 cacagtcacg ttattgttta cataaatgat tttttttact attcaaactt actctgtttg
     9481 tgtactccca ctggtatagc cttcttttat cttttctggt tcaggctcta tcactttact
     9541 aggtacggca tctgcgttga gtcgcctcct tttaaatgtc tgaccttttg caggtgcagc
     9601 cttccactgc gaatcattaa agtgggtatc acaaatttgg gagttttcac caaggctgca
     9661 cccaaggctc tgctcccaca attttctctt aatagcacac ttcggcacgt gaattaattt
     9721 tactccagtc acagctttgc agcaaaattt gcaatatttc attttttttt attccacgta
     9781 agggttaatg ttttcaaaaa aaaattcgtc cgcacacaac ctttcctctc aacaagcaaa
     9841 cgtgcactga atttaagtgt atacttcggt aagcttcggc tatcgacggg accaccttat
     9901 gttatttcat catgggccag acccacgtag tcc
//
