LOCUS       sequence-345646-        5846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0787:Dendra
FEATURES             Location/Qualifiers
     source          1..5846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     758..777
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        758..776
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2502..2525
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2782..3582
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3707..4295
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4196..4215
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acatgaagga ccgtacgaag atgaacaagg gccccgaggc attcctggtg accacgacgc
       61 agggcaaccg gctgtcgaag tccgcgttca ccaagtcgct gaagcgtggc tacgccaaga
      121 tcggtcggcc ggaactccgc atccacgacc tccgcgctgt cggcgctacg ttcgccgctc
      181 aggcaggtgc gacgaccaag gagctgatgg cccgtctcgg tcacacgact cctaggatgg
      241 cgatgaagta ccagatggcg tctgaggccc gcgacgaggc tatcgctgag gcgatgtcca
      301 agctggccaa gacctcctga aacgcaaaaa gcccccctcc caaggacact gagtcctaaa
      361 gaggggggtt tcttgtcagt acgcgaagaa ccacgcctgg ccgcgagcgc cagcaccgcc
      421 gctctgtgcg gagacctggg caccagcccc gccgccgcca ggagcattgc cgttcccgcc
      481 agaaatctag acggtgacca caacgcgccc gctttgatcg gggacgtcga aatattggat
      541 cgtcggcacc gtcacggccg tgggaggcgg cacgatccgc gacgtgatga tcggccgcat
      601 ccccacggtg ctgcgcagtg agctctacgc catcccggcg ttgatctgtg cgttcgcacg
      661 cacaggcccg gtgtgagaag ggtctctgac gagcgggaga actccctatc agtgatagag
      721 tttgtcctcc ctatcagtga tagataggct taaggagtaa tacgactcac tatagggaga
      781 aggaggccat tatgaagcgc cttctgatgc tgctcgtgag caccctgacc gcgatgagcc
      841 tggtcgggtg cagtcaggcc gcgccggcgc tgaacctgcc gtcgttgacg ctcgcaccac
      901 cgacgcccgc tggcatggaa gagatcccgc cgcagcccgt gcggcttccg gagatcgaca
      961 acgacgactg caaccgcacc gcgagcctgc gcccgttcag caacaaggaa gaggccgagg
     1021 cggccgtcgc caacatccgc gcgcgaggcc ggctgatcgt cggcctggac atcggcagca
     1081 acctgttctc gttccgcgat ccgatcaccg ggcagatcac cgggttcgac gtcgacatcg
     1141 ccggcgaggt gtcgagggac atcttcggca cgccgtcgaa ggtcgagtac cgcatcctgt
     1201 cgtcggccga ccggatcacc gcgctgcgca acaaccaggt ggacatcgtc gtcaagacca
     1261 tgaccatcac ctgcgagcgc aagaagctgg tgaacttctc cacggtgtac ctgatggcca
     1321 accagcggat cctggcctcg cgtgactcga acatctcgca ggcctcggat ctctcgggcc
     1381 ggcgcgtgtg cgtcgtcgac ggcaccacct cgctgcagcg gatccagcag atcagcccct
     1441 cgcccatcat cgtgtcggtg gtgacgtggg ccgattgcct tgtggcgctg cagcagcggc
     1501 aggccgatgc ggtcagcacc gacgactcga tcctcgccgg cctggtggcc caggacccgt
     1561 acctgcacat cgtcgggccc agcctgagcc aggagcccta cggcatcggg gtgaacctgg
     1621 agaacaccgg cctggtgcgc gtggtgaacg ggacgttgga gcgcatccgc cgtgacggca
     1681 cgtggtacac cctctaccgc aagtggttga cggtgctggg cccggcgccg gcacctcccg
     1741 tggcgaggta cgtcgacaag cttggtgcgg ccgcgggcaa cacgcctggc atcaatctga
     1801 tcaaggagga catgcgtgtc aaggtgcata tggagggcaa cgtgaacggt cacgccttcg
     1861 tgatcgaggg ggagggtaag ggtaagcctt acgaagggac ccagaccgcc aacctcaccg
     1921 tcaaggaggg cgcccctctc ccattctcgt acgatatcct cactaccgcg gtgcattacg
     1981 ggaaccgcgt gttcaccaag taccccgagg atatccctga ctacttcaag cagagcttcc
     2041 cggagggata ctcctgggag cggacgatga ccttcgaaga caagggtatc tgtacgatcc
     2101 gcagcgacat ctcgctggaa ggggactgtt tcttccagaa cgtccgtttc aaggggacca
     2161 actttccgcc gaacggcccg gtcatgcaga agaagaccct caagtgggag ccgagcacgg
     2221 agaagctgca cgttcgggac ggcctcctgg tcggcaacat caacatggcg ctgctgctgg
     2281 aaggcggcgg ccactaccta tgcgacttca agaccacgta caaagcgaag aaggtcgtcc
     2341 agctgcccga cgcgcacttc gtggaccacc gcatcgaaat cctggggaac gattcggact
     2401 acaacaaggt caagttgtac gagcacgctg tggcacgcta tagcccgctc ccgtcgcagg
     2461 tctggttcga aggcggcggc ggggagaacc tgtatttcca ggactacaag gacgacgacg
     2521 acaagtgatt taaataaccc cttggggcct ctaaacgggt cttgaggggt tttttgttaa
     2581 ctagcgtacg atcgaattcc tgcagctcac ggtaactgat gccgtatttg cagtaccagc
     2641 gtacggccca cagaatgatg tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca
     2701 gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt
     2761 tgatcgtgct atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa
     2821 tggcgaaaag ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg
     2881 ctgctggtcc acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg
     2941 atcgaggccc tgcgtgctac gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca
     3001 ggtctggacg acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt
     3061 gtctctgaca cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt
     3121 gcggcagcgg ggccacaggc agagcagatc atctctgatc cattgcccct gccaccttac
     3181 tcgcctgcaa gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc
     3241 gtgggacacg atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat
     3301 ggggtgccga gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc
     3361 gagaatgacc actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc
     3421 cttcagaagg aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt
     3481 gtggcgacag ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag
     3541 gcgggatgcg aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga
     3601 acgagatgac gttggagggg caaggtcgcg ctgattgctg gggcaacacg ggggatccac
     3661 tagttccatg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3721 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3781 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3841 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3901 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3961 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4021 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4081 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaggggaga aaggcggaca
     4141 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4201 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4261 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4321 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4381 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4441 ccgagcgcaa cgcgtgcggc cgcggtaccc ggggatcctc tagagtcgac caccaagggc
     4501 accatctctg cttgggccac cccgttggcc gcagccagct cgctgagagc cgtgaacgac
     4561 agggcgaacg ccagcccgcc gacggcgagg gttccgaccg ctgcaactcc cggtgcaacc
     4621 ttgtcccggt ctattctctt cactgcacca gctccaatct ggtgtgaatg cccctcgtct
     4681 gttcgcgcag gcggggggct ctattcgttt gtcagcatcg aaagtagcca gatcagggat
     4741 gcgttgcaac cgcgtatgcc caggtcagaa gagtcgcaca agagttgcag acccctggaa
     4801 agaaaaatgg ccagagggcg aaaacaccct ctgaccagcg gagcgggcga cgggaatcga
     4861 acccgcgtag ctagtttgga agaatgggtg tctgccgacc acatatgggc cggtcaagat
     4921 aggtttttac cccctctcgg ctgcatcctc taagtggaaa gaaattgcag gtcgtagaag
     4981 cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg tagccttgtt cacgacgaga
     5041 ggagacctag ttggcacgtc gcggatgggg atcgctgaag actcagcgca gcgggaggat
     5101 ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac tacgcgctgc agacctacga
     5161 caacaagatg gacgccgaag cctggctcgc gggcgagaag cggctcatcg agatggagac
     5221 ctggacccct ccacaggacc gggcgaagaa ggcagccgcc agcgccatca cgctggagga
     5281 gtacacccgg aagtggctcg tggagcgcga cctcgcagac ggcaccaggg atctgtacag
     5341 cgggcacgcg gagcgccgca tctacccggt gctaggtgaa gtggcggtca cagagatgac
     5401 gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg aagcacccga ctgcccgccg
     5461 gcatgcctac aacgtcctcc gggcggtgat gaacacagcg gtcgaggaca agctgatcgc
     5521 agagaacccg tgccggatcg agcagaaggc agccgatgag cgcgacgtag aggcgctgac
     5581 gcctgaggag ctggacatcg tcgccgctga gatcttcgag cactaccgga tcgcggcata
     5641 catcctggcg tggacgagcc tccggttcgg agagctgatc gagcttcgcc gcaaggacat
     5701 cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt ggcgcttccc gcgtggggaa
     5761 caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag cgtcctgtga cggttccgcc
     5821 tcacgtcgcg gagatgatcc gagcgc
//
