LOCUS       sequence-345647-        5588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0835:Dendra
FEATURES             Location/Qualifiers
     source          1..5588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     749..768
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        749..767
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2235..2258
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2515..3315
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3440..4028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3929..3948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgtacgaa gatgaacaag ggccccgagg cattcctggt gaccacgacg cagggcaacc
       61 ggctgtcgaa gtccgcgttc accaagtcgc tgaagcgtgg ctacgccaag atcggtcggc
      121 cggaactccg catccacgac ctccgcgctg tcggcgctac gttcgccgct caggcaggtg
      181 cgacgaccaa ggagctgatg gcccgtctcg gtcacacgac tcctaggatg gcgatgaagt
      241 accagatggc gtctgaggcc cgcgacgagg ctatcgctga ggcgatgtcc aagctggcca
      301 agacctcctg aaacgcaaaa agcccccctc ccaaggacac tgagtcctaa agaggggggt
      361 ttcttgtcag tacgcgaaga accacgcctg gccgcgagcg ccagcaccgc cgctctgtgc
      421 ggagacctgg gcaccagccc cgccgccgcc aggagcattg ccgttcccgc cagaaatcta
      481 gacggtgacc acaacgcgcc cgctttgatc ggggacgtcg aaatattgga tcgtcggcac
      541 cgtcacggcc gtgggaggcg gcacgatccg cgacgtgatg atcggccgca tccccacggt
      601 gctgcgcagt gagctctacg ccatcccggc gttgatctgt gcgttcgcac gcacaggccc
      661 ggtgtgagaa gggtctctga cgagcgggag aactccctat cagtgataga gtttgtcctc
      721 cctatcagtg atagataggc ttaaggagta atacgactca ctatagggag aaggaggcca
      781 ttatgctgaa gcccgtcagt gttgccgtcc tgttcgccac gcccgtcctc gcgttgagcg
      841 cctgcagccc tcccggtgaa acggcgtcga gcgagcccgg aaccaccccc gcgatctgga
      901 ccggatcgcc gtctcccgcg gcgccgtccg gggaagacca cggtggcggg cacggtgccg
      961 gcgcggcagg cgccggtgag accctcaccg cagaactcaa gaccgccgac ggaacctcgg
     1021 tggcaaccgc cgacttccag ttcgccgacg gctttgccac ggtgacgatc gagaccacca
     1081 ccccgggtcg cctcaccccc ggcttccacg gtgtgcacat tcactcggtg ggcaagtgcg
     1141 aggcgaactc cgtcgccccg accggtggcg cgcccggtga cttcaactcc gccggcggcc
     1201 acttccaggt gtccggccac agcggacatc ccgccagcgg cgacctgagc tcgctgcagg
     1261 tccgggccga cggctcgggc aagctggtga ccaccaccga tgcgttcacg gccgaggatc
     1321 tgctcgacgg cgccaagacc gcgatcatca tccacgagaa ggccgacaac ttcgccaaca
     1381 tcccgccgga gcgctaccag caggtcaacg gcgcacccgg cccggatcag acgacgatgg
     1441 ccaccggcga cgccggaagt cgggtggcgt gcggtgtcat ctctgccggc aagcttggtg
     1501 cggccgcggg caacacgcct ggcatcaatc tgatcaagga ggacatgcgt gtcaaggtgc
     1561 atatggaggg caacgtgaac ggtcacgcct tcgtgatcga gggggagggt aagggtaagc
     1621 cttacgaagg gacccagacc gccaacctca ccgtcaagga gggcgcccct ctcccattct
     1681 cgtacgatat cctcactacc gcggtgcatt acgggaaccg cgtgttcacc aagtaccccg
     1741 aggatatccc tgactacttc aagcagagct tcccggaggg atactcctgg gagcggacga
     1801 tgaccttcga agacaagggt atctgtacga tccgcagcga catctcgctg gaaggggact
     1861 gtttcttcca gaacgtccgt ttcaagggga ccaactttcc gccgaacggc ccggtcatgc
     1921 agaagaagac cctcaagtgg gagccgagca cggagaagct gcacgttcgg gacggcctcc
     1981 tggtcggcaa catcaacatg gcgctgctgc tggaaggcgg cggccactac ctatgcgact
     2041 tcaagaccac gtacaaagcg aagaaggtcg tccagctgcc cgacgcgcac ttcgtggacc
     2101 accgcatcga aatcctgggg aacgattcgg actacaacaa ggtcaagttg tacgagcacg
     2161 ctgtggcacg ctatagcccg ctcccgtcgc aggtctggtt cgaaggcggc ggcggggaga
     2221 acctgtattt ccaggactac aaggacgacg acgacaagtg atttaaataa ccccttgggg
     2281 cctctaaacg ggtcttgagg ggttttttgt taactagcgt acgatcgaat tcctgcagct
     2341 cacggtaact gatgccgtat ttgcagtacc agcgtacggc ccacagaatg atgtcacgct
     2401 gaaaatgccg gcctttgaat gggttcatgt gcagctccat cagcaaaagg ggatgataag
     2461 tttatcacca ccgactattt gcaacagtgc cgttgatcgt gctatgatcg actgatgtca
     2521 tcagcggtgg agtgcaatgt cgtgcaatac gaatggcgaa aagccgagct catcggtcag
     2581 cttctcaacc ttggggttac ccccggcggt gtgctgctgg tccacagctc cttccgtagc
     2641 gtccggcccc tcgaagatgg gccacttgga ctgatcgagg ccctgcgtgc tacgctgggt
     2701 ccgggaggga cgctcgtcat gccctcgtgg tcaggtctgg acgacgagcc gttcgatcct
     2761 gccacgtcgc ccgttacacc ggaccttgga gttgtctctg acacattctg gcgcctgcca
     2821 aatgtaaagc gcagcgccca tccatttgcc tttgcggcag cggggccaca ggcagagcag
     2881 atcatctctg atccattgcc cctgccacct tactcgcctg caagcccggt cgcccgtgtc
     2941 catgaactcg atgggcaggt acttctcctc ggcgtgggac acgatgccaa cacgacgctg
     3001 catcttgccg agttgatggc aaaggttccc tatggggtgc cgagacactg caccattctt
     3061 caggatggca agttggtacg cgtcgattat ctcgagaatg accactgctg tgagcgcttt
     3121 gccttggcgg acaggtggct caaggagaag agccttcaga aggaaggtcc agtcggtcat
     3181 gcctttgctc ggttgatccg ctcccgcgac attgtggcga cagccctggg tcaactgggc
     3241 cgagatccgt tgatcttcct gcatccgcca gaggcgggat gcgaagaatg cgatgccgct
     3301 cgccagtcga ttggctgagc tcatgagcgg agaacgagat gacgttggag gggcaaggtc
     3361 gcgctgattg ctggggcaac acgggggatc cactagttcc atgagcgtca gaccccgtag
     3421 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3481 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3541 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     3601 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3661 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3721 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3781 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3841 gcgccacgct tcccgagggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3901 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3961 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     4021 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     4081 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     4141 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg caacgcgtgc ggccgcggta
     4201 cccggggatc ctctagagtc gaccaccaag ggcaccatct ctgcttgggc caccccgttg
     4261 gccgcagcca gctcgctgag agccgtgaac gacagggcga acgccagccc gccgacggcg
     4321 agggttccga ccgctgcaac tcccggtgca accttgtccc ggtctattct cttcactgca
     4381 ccagctccaa tctggtgtga atgcccctcg tctgttcgcg caggcggggg gctctattcg
     4441 tttgtcagca tcgaaagtag ccagatcagg gatgcgttgc aaccgcgtat gcccaggtca
     4501 gaagagtcgc acaagagttg cagacccctg gaaagaaaaa tggccagagg gcgaaaacac
     4561 cctctgacca gcggagcggg cgacgggaat cgaacccgcg tagctagttt ggaagaatgg
     4621 gtgtctgccg accacatatg ggccggtcaa gataggtttt taccccctct cggctgcatc
     4681 ctctaagtgg aaagaaattg caggtcgtag aagcgcgttg aagcctgaga gttgcacagg
     4741 agttgcaacc cggtagcctt gttcacgacg agaggagacc tagttggcac gtcgcggatg
     4801 gggatcgctg aagactcagc gcagcgggag gatccaagcc tcatacgtca acccgcagga
     4861 cggtgtgagg tactacgcgc tgcagaccta cgacaacaag atggacgccg aagcctggct
     4921 cgcgggcgag aagcggctca tcgagatgga gacctggacc cctccacagg accgggcgaa
     4981 gaaggcagcc gccagcgcca tcacgctgga ggagtacacc cggaagtggc tcgtggagcg
     5041 cgacctcgca gacggcacca gggatctgta cagcgggcac gcggagcgcc gcatctaccc
     5101 ggtgctaggt gaagtggcgg tcacagagat gacgccagct ctggtgcgtg cgtggtgggc
     5161 cgggatgggt aggaagcacc cgactgcccg ccggcatgcc tacaacgtcc tccgggcggt
     5221 gatgaacaca gcggtcgagg acaagctgat cgcagagaac ccgtgccgga tcgagcagaa
     5281 ggcagccgat gagcgcgacg tagaggcgct gacgcctgag gagctggaca tcgtcgccgc
     5341 tgagatcttc gagcactacc ggatcgcggc atacatcctg gcgtggacga gcctccggtt
     5401 cggagagctg atcgagcttc gccgcaagga catcgtggac gacggcatga cgatgaagct
     5461 ccgggtgcgc cgtggcgctt cccgcgtggg gaacaagatc gtcgttggca acgccaagac
     5521 cgtccggtcg aagcgtcctg tgacggttcc gcctcacgtc gcggagatga tccgagcgca
     5581 catgaagg
//
