LOCUS       sequence-345648-        5438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0905:Dendra
FEATURES             Location/Qualifiers
     source          1..5438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     728..747
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        728..746
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1278..1287
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2064..2087
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2344..3144
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3269..3857
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3758..3777
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccccgaggc attcctggtg accacgacgc agggcaaccg gctgtcgaag tccgcgttca
       61 ccaagtcgct gaagcgtggc tacgccaaga tcggtcggcc ggaactccgc atccacgacc
      121 tccgcgctgt cggcgctacg ttcgccgctc aggcaggtgc gacgaccaag gagctgatgg
      181 cccgtctcgg tcacacgact cctaggatgg cgatgaagta ccagatggcg tctgaggccc
      241 gcgacgaggc tatcgctgag gcgatgtcca agctggccaa gacctcctga aacgcaaaaa
      301 gcccccctcc caaggacact gagtcctaaa gaggggggtt tcttgtcagt acgcgaagaa
      361 ccacgcctgg ccgcgagcgc cagcaccgcc gctctgtgcg gagacctggg caccagcccc
      421 gccgccgcca ggagcattgc cgttcccgcc agaaatctag acggtgacca caacgcgccc
      481 gctttgatcg gggacgtcga aatattggat cgtcggcacc gtcacggccg tgggaggcgg
      541 cacgatccgc gacgtgatga tcggccgcat ccccacggtg ctgcgcagtg agctctacgc
      601 catcccggcg ttgatctgtg cgttcgcacg cacaggcccg gtgtgagaag ggtctctgac
      661 gagcgggaga actccctatc agtgatagag tttgtcctcc ctatcagtga tagataggct
      721 taaggagtaa tacgactcac tatagggaga aggaggccat tatgacccgc gaccatcaga
      781 ccgagcccgc ccgtatcccc gccacccgca gcctgcgcgc tctcgggccg atcggctgga
      841 tcgcggcccg ggcgggggca cgcgcgatcc gggcaccgcg ctttcacctg ttcgacgtca
      901 tcggccagca caagctggcg ttcctggcgt ttctgccgta ctccggtgtg ctgctgaact
      961 ggggcaagct gcccaagcgc gacaccgaac tggtcatcct gcgcgtggcc cacctgcgcg
     1021 gcagcaccta cgaactgcag cagcaccgcc gactggcccg cagccgcggc ctcgacaccg
     1081 aaacacaggc caaaatcttc gaggggcccg acgccgaagg cctgaccgat cgtcagcgca
     1141 cgctgatcac cgcgaccgac gagttcgtgg tcacgcgctc gatgtcaccg gagacctggg
     1201 cggcgctgtc cacgcacctc aaccgggcgc agctgatcga attctgtttg ctcgcagggc
     1261 attacgacgg ccttgccgcc accatggcga ccctgcgcat cccactcgac ttccccgaca
     1321 agcttggtgc ggccgcgggc aacacgcctg gcatcaatct gatcaaggag gacatgcgtg
     1381 tcaaggtgca tatggagggc aacgtgaacg gtcacgcctt cgtgatcgag ggggagggta
     1441 agggtaagcc ttacgaaggg acccagaccg ccaacctcac cgtcaaggag ggcgcccctc
     1501 tcccattctc gtacgatatc ctcactaccg cggtgcatta cgggaaccgc gtgttcacca
     1561 agtaccccga ggatatccct gactacttca agcagagctt cccggaggga tactcctggg
     1621 agcggacgat gaccttcgaa gacaagggta tctgtacgat ccgcagcgac atctcgctgg
     1681 aaggggactg tttcttccag aacgtccgtt tcaaggggac caactttccg ccgaacggcc
     1741 cggtcatgca gaagaagacc ctcaagtggg agccgagcac ggagaagctg cacgttcggg
     1801 acggcctcct ggtcggcaac atcaacatgg cgctgctgct ggaaggcggc ggccactacc
     1861 tatgcgactt caagaccacg tacaaagcga agaaggtcgt ccagctgccc gacgcgcact
     1921 tcgtggacca ccgcatcgaa atcctgggga acgattcgga ctacaacaag gtcaagttgt
     1981 acgagcacgc tgtggcacgc tatagcccgc tcccgtcgca ggtctggttc gaaggcggcg
     2041 gcggggagaa cctgtatttc caggactaca aggacgacga cgacaagtga tttaaataac
     2101 cccttggggc ctctaaacgg gtcttgaggg gttttttgtt aactagcgta cgatcgaatt
     2161 cctgcagctc acggtaactg atgccgtatt tgcagtacca gcgtacggcc cacagaatga
     2221 tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg cagctccatc agcaaaaggg
     2281 gatgataagt ttatcaccac cgactatttg caacagtgcc gttgatcgtg ctatgatcga
     2341 ctgatgtcat cagcggtgga gtgcaatgtc gtgcaatacg aatggcgaaa agccgagctc
     2401 atcggtcagc ttctcaacct tggggttacc cccggcggtg tgctgctggt ccacagctcc
     2461 ttccgtagcg tccggcccct cgaagatggg ccacttggac tgatcgaggc cctgcgtgct
     2521 acgctgggtc cgggagggac gctcgtcatg ccctcgtggt caggtctgga cgacgagccg
     2581 ttcgatcctg ccacgtcgcc cgttacaccg gaccttggag ttgtctctga cacattctgg
     2641 cgcctgccaa atgtaaagcg cagcgcccat ccatttgcct ttgcggcagc ggggccacag
     2701 gcagagcaga tcatctctga tccattgccc ctgccacctt actcgcctgc aagcccggtc
     2761 gcccgtgtcc atgaactcga tgggcaggta cttctcctcg gcgtgggaca cgatgccaac
     2821 acgacgctgc atcttgccga gttgatggca aaggttccct atggggtgcc gagacactgc
     2881 accattcttc aggatggcaa gttggtacgc gtcgattatc tcgagaatga ccactgctgt
     2941 gagcgctttg ccttggcgga caggtggctc aaggagaaga gccttcagaa ggaaggtcca
     3001 gtcggtcatg cctttgctcg gttgatccgc tcccgcgaca ttgtggcgac agccctgggt
     3061 caactgggcc gagatccgtt gatcttcctg catccgccag aggcgggatg cgaagaatgc
     3121 gatgccgctc gccagtcgat tggctgagct catgagcgga gaacgagatg acgttggagg
     3181 ggcaaggtcg cgctgattgc tggggcaaca cgggggatcc actagttcca tgagcgtcag
     3241 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3301 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3361 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     3421 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3481 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3541 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3601 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3661 tatgagaaag cgccacgctt cccgagggga gaaaggcgga caggtatccg gtaagcggca
     3721 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3781 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3841 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3901 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3961 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc aacgcgtgcg
     4021 gccgcggtac ccggggatcc tctagagtcg accaccaagg gcaccatctc tgcttgggcc
     4081 accccgttgg ccgcagccag ctcgctgaga gccgtgaacg acagggcgaa cgccagcccg
     4141 ccgacggcga gggttccgac cgctgcaact cccggtgcaa ccttgtcccg gtctattctc
     4201 ttcactgcac cagctccaat ctggtgtgaa tgcccctcgt ctgttcgcgc aggcgggggg
     4261 ctctattcgt ttgtcagcat cgaaagtagc cagatcaggg atgcgttgca accgcgtatg
     4321 cccaggtcag aagagtcgca caagagttgc agacccctgg aaagaaaaat ggccagaggg
     4381 cgaaaacacc ctctgaccag cggagcgggc gacgggaatc gaacccgcgt agctagtttg
     4441 gaagaatggg tgtctgccga ccacatatgg gccggtcaag ataggttttt accccctctc
     4501 ggctgcatcc tctaagtgga aagaaattgc aggtcgtaga agcgcgttga agcctgagag
     4561 ttgcacagga gttgcaaccc ggtagccttg ttcacgacga gaggagacct agttggcacg
     4621 tcgcggatgg ggatcgctga agactcagcg cagcgggagg atccaagcct catacgtcaa
     4681 cccgcaggac ggtgtgaggt actacgcgct gcagacctac gacaacaaga tggacgccga
     4741 agcctggctc gcgggcgaga agcggctcat cgagatggag acctggaccc ctccacagga
     4801 ccgggcgaag aaggcagccg ccagcgccat cacgctggag gagtacaccc ggaagtggct
     4861 cgtggagcgc gacctcgcag acggcaccag ggatctgtac agcgggcacg cggagcgccg
     4921 catctacccg gtgctaggtg aagtggcggt cacagagatg acgccagctc tggtgcgtgc
     4981 gtggtgggcc gggatgggta ggaagcaccc gactgcccgc cggcatgcct acaacgtcct
     5041 ccgggcggtg atgaacacag cggtcgagga caagctgatc gcagagaacc cgtgccggat
     5101 cgagcagaag gcagccgatg agcgcgacgt agaggcgctg acgcctgagg agctggacat
     5161 cgtcgccgct gagatcttcg agcactaccg gatcgcggca tacatcctgg cgtggacgag
     5221 cctccggttc ggagagctga tcgagcttcg ccgcaaggac atcgtggacg acggcatgac
     5281 gatgaagctc cgggtgcgcc gtggcgcttc ccgcgtgggg aacaagatcg tcgttggcaa
     5341 cgccaagacc gtccggtcga agcgtcctgt gacggttccg cctcacgtcg cggagatgat
     5401 ccgagcgcac atgaaggacc gtacgaagat gaacaagg
//
