LOCUS       sequence-345649-        5969 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0928:Dendra
FEATURES             Location/Qualifiers
     source          1..5969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     751..770
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        751..769
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2618..2641
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2898..3698
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3823..4411
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4312..4331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggaccgtacg aagatgaaca agggccccga ggcattcctg gtgaccacga cgcagggcaa
       61 ccggctgtcg aagtccgcgt tcaccaagtc gctgaagcgt ggctacgcca agatcggtcg
      121 gccggaactc cgcatccacg acctccgcgc tgtcggcgct acgttcgccg ctcaggcagg
      181 tgcgacgacc aaggagctga tggcccgtct cggtcacacg actcctagga tggcgatgaa
      241 gtaccagatg gcgtctgagg cccgcgacga ggctatcgct gaggcgatgt ccaagctggc
      301 caagacctcc tgaaacgcaa aaagcccccc tcccaaggac actgagtcct aaagaggggg
      361 gtttcttgtc agtacgcgaa gaaccacgcc tggccgcgag cgccagcacc gccgctctgt
      421 gcggagacct gggcaccagc cccgccgccg ccaggagcat tgccgttccc gccagaaatc
      481 tagacggtga ccacaacgcg cccgctttga tcggggacgt cgaaatattg gatcgtcggc
      541 accgtcacgg ccgtgggagg cggcacgatc cgcgacgtga tgatcggccg catccccacg
      601 gtgctgcgca gtgagctcta cgccatcccg gcgttgatct gtgcgttcgc acgcacaggc
      661 ccggtgtgag aagggtctct gacgagcggg agaactccct atcagtgata gagtttgtcc
      721 tccctatcag tgatagatag gcttaaggag taatacgact cactataggg agaaggaggc
      781 cattatggcc agttcgcatg ttgccggcgt ggagatcgcg gaggcggttc cgctggcccc
      841 gctgaccacg ctgcggatcg ggcccgtggc gcggcggatg ctcacatgca ccagcaccga
      901 acagctcatc ggggtgttgc gcgcactgac cgccgacgac gagccgctgc tgatcctcgc
      961 cggcgggtcc aacgtggtgc tggccgacga cctgaccgat ctcacggtcg tgcgcatcgc
     1021 caacaccgag atcaccgtcg acggcgaccg tgtgcgtgcc gaggccggcg cgctgtggga
     1081 cgacgtcgtg gtgacctcgc tcgcgcacgg cctgggcggg ctggagtgcc tgtcgggcat
     1141 ccccggctcg gccggtgcga cgccggtgca gaacgtcggc gcgtacggcg ccgaggtcgc
     1201 cgacaccatc acacgggtgc ggctgctgga ccgtcgtacc ggtgaagacc gttgggcgac
     1261 aaccgaagag ctgcgcttcg gataccgcac gagcgtgctg aaacactccg acgcggtcat
     1321 cgtgctcgag gtcgagttcg gtctggacgc cgcgggtcgc agcgccccgg tgcggtaccg
     1381 cgaactggcg acggcgctgg gcgtggaacc gggggagcgc accgacccgc tgcgcgtgcg
     1441 cgacgccgtg ctgcggctgc ggaccggcaa gggcatggtg gtcgaccccg atccggacca
     1501 tcccgaccac gacacctgga gcgtcggctc gttcttcacc aaccccgtcg tgacgcacgc
     1561 cgacttcgag cgcgtcgagc gcatcgcgcg cgatgcgggc gccgggccgg tgcccaacta
     1621 tcccgcgccg gacggggtga aactcgccgc gggctggctc gtggagcgcg cgggcttcgg
     1681 caagggctac cccggtgagg gggcgcgggc acggctgtcc accaagcatg cgctggcgct
     1741 gaccaaccgc ggccaggcca ccacggccga cgtgatggcc ctggcaggca cggtccgggc
     1801 gggcgtgctc gacgtgttcg ggatcgaact cacaccagaa ccgattctgc tggggtgcgt
     1861 gctgtcggta cccaagcttg gtgcggccgc gggcaacacg cctggcatca atctgatcaa
     1921 ggaggacatg cgtgtcaagg tgcatatgga gggcaacgtg aacggtcacg ccttcgtgat
     1981 cgagggggag ggtaagggta agccttacga agggacccag accgccaacc tcaccgtcaa
     2041 ggagggcgcc cctctcccat tctcgtacga tatcctcact accgcggtgc attacgggaa
     2101 ccgcgtgttc accaagtacc ccgaggatat ccctgactac ttcaagcaga gcttcccgga
     2161 gggatactcc tgggagcgga cgatgacctt cgaagacaag ggtatctgta cgatccgcag
     2221 cgacatctcg ctggaagggg actgtttctt ccagaacgtc cgtttcaagg ggaccaactt
     2281 tccgccgaac ggcccggtca tgcagaagaa gaccctcaag tgggagccga gcacggagaa
     2341 gctgcacgtt cgggacggcc tcctggtcgg caacatcaac atggcgctgc tgctggaagg
     2401 cggcggccac tacctatgcg acttcaagac cacgtacaaa gcgaagaagg tcgtccagct
     2461 gcccgacgcg cacttcgtgg accaccgcat cgaaatcctg gggaacgatt cggactacaa
     2521 caaggtcaag ttgtacgagc acgctgtggc acgctatagc ccgctcccgt cgcaggtctg
     2581 gttcgaaggc ggcggcgggg agaacctgta tttccaggac tacaaggacg acgacgacaa
     2641 gtgatttaaa taaccccttg gggcctctaa acgggtcttg aggggttttt tgttaactag
     2701 cgtacgatcg aattcctgca gctcacggta actgatgccg tatttgcagt accagcgtac
     2761 ggcccacaga atgatgtcac gctgaaaatg ccggcctttg aatgggttca tgtgcagctc
     2821 catcagcaaa aggggatgat aagtttatca ccaccgacta tttgcaacag tgccgttgat
     2881 cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc
     2941 gaaaagccga gctcatcggt cagcttctca accttggggt tacccccggc ggtgtgctgc
     3001 tggtccacag ctccttccgt agcgtccggc ccctcgaaga tgggccactt ggactgatcg
     3061 aggccctgcg tgctacgctg ggtccgggag ggacgctcgt catgccctcg tggtcaggtc
     3121 tggacgacga gccgttcgat cctgccacgt cgcccgttac accggacctt ggagttgtct
     3181 ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg
     3241 cagcggggcc acaggcagag cagatcatct ctgatccatt gcccctgcca ccttactcgc
     3301 ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg
     3361 gacacgatgc caacacgacg ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg
     3421 tgccgagaca ctgcaccatt cttcaggatg gcaagttggt acgcgtcgat tatctcgaga
     3481 atgaccactg ctgtgagcgc tttgccttgg cggacaggtg gctcaaggag aagagccttc
     3541 agaaggaagg tccagtcggt catgcctttg ctcggttgat ccgctcccgc gacattgtgg
     3601 cgacagccct gggtcaactg ggccgagatc cgttgatctt cctgcatccg ccagaggcgg
     3661 gatgcgaaga atgcgatgcc gctcgccagt cgattggctg agctcatgag cggagaacga
     3721 gatgacgttg gaggggcaag gtcgcgctga ttgctggggc aacacggggg atccactagt
     3781 tccatgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3841 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3901 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3961 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4021 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4081 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4141 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4201 cctacagcgt gagctatgag aaagcgccac gcttcccgag gggagaaagg cggacaggta
     4261 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4321 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4381 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4441 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4501 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4561 gcgcaacgcg tgcggccgcg gtacccgggg atcctctaga gtcgaccacc aagggcacca
     4621 tctctgcttg ggccaccccg ttggccgcag ccagctcgct gagagccgtg aacgacaggg
     4681 cgaacgccag cccgccgacg gcgagggttc cgaccgctgc aactcccggt gcaaccttgt
     4741 cccggtctat tctcttcact gcaccagctc caatctggtg tgaatgcccc tcgtctgttc
     4801 gcgcaggcgg ggggctctat tcgtttgtca gcatcgaaag tagccagatc agggatgcgt
     4861 tgcaaccgcg tatgcccagg tcagaagagt cgcacaagag ttgcagaccc ctggaaagaa
     4921 aaatggccag agggcgaaaa caccctctga ccagcggagc gggcgacggg aatcgaaccc
     4981 gcgtagctag tttggaagaa tgggtgtctg ccgaccacat atgggccggt caagataggt
     5041 ttttaccccc tctcggctgc atcctctaag tggaaagaaa ttgcaggtcg tagaagcgcg
     5101 ttgaagcctg agagttgcac aggagttgca acccggtagc cttgttcacg acgagaggag
     5161 acctagttgg cacgtcgcgg atggggatcg ctgaagactc agcgcagcgg gaggatccaa
     5221 gcctcatacg tcaacccgca ggacggtgtg aggtactacg cgctgcagac ctacgacaac
     5281 aagatggacg ccgaagcctg gctcgcgggc gagaagcggc tcatcgagat ggagacctgg
     5341 acccctccac aggaccgggc gaagaaggca gccgccagcg ccatcacgct ggaggagtac
     5401 acccggaagt ggctcgtgga gcgcgacctc gcagacggca ccagggatct gtacagcggg
     5461 cacgcggagc gccgcatcta cccggtgcta ggtgaagtgg cggtcacaga gatgacgcca
     5521 gctctggtgc gtgcgtggtg ggccgggatg ggtaggaagc acccgactgc ccgccggcat
     5581 gcctacaacg tcctccgggc ggtgatgaac acagcggtcg aggacaagct gatcgcagag
     5641 aacccgtgcc ggatcgagca gaaggcagcc gatgagcgcg acgtagaggc gctgacgcct
     5701 gaggagctgg acatcgtcgc cgctgagatc ttcgagcact accggatcgc ggcatacatc
     5761 ctggcgtgga cgagcctccg gttcggagag ctgatcgagc ttcgccgcaa ggacatcgtg
     5821 gacgacggca tgacgatgaa gctccgggtg cgccgtggcg cttcccgcgt ggggaacaag
     5881 atcgtcgttg gcaacgccaa gaccgtccgg tcgaagcgtc ctgtgacggt tccgcctcac
     5941 gtcgcggaga tgatccgagc gcacatgaa
//
