LOCUS       sequence-345650-        5849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0939:Dendra
FEATURES             Location/Qualifiers
     source          1..5849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     646..665
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        646..664
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2393..2416
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2673..3473
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3598..4186
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4087..4106
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgccaagatc ggtcggccgg aactccgcat ccacgacctc cgcgctgtcg gcgctacgtt
       61 cgccgctcag gcaggtgcga cgaccaagga gctgatggcc cgtctcggtc acacgactcc
      121 taggatggcg atgaagtacc agatggcgtc tgaggcccgc gacgaggcta tcgctgaggc
      181 gatgtccaag ctggccaaga cctcctgaaa cgcaaaaagc ccccctccca aggacactga
      241 gtcctaaaga ggggggtttc ttgtcagtac gcgaagaacc acgcctggcc gcgagcgcca
      301 gcaccgccgc tctgtgcgga gacctgggca ccagccccgc cgccgccagg agcattgccg
      361 ttcccgccag aaatctagac ggtgaccaca acgcgcccgc tttgatcggg gacgtcgaaa
      421 tattggatcg tcggcaccgt cacggccgtg ggaggcggca cgatccgcga cgtgatgatc
      481 ggccgcatcc ccacggtgct gcgcagtgag ctctacgcca tcccggcgtt gatctgtgcg
      541 ttcgcacgca caggcccggt gtgagaaggg tctctgacga gcgggagaac tccctatcag
      601 tgatagagtt tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta
      661 tagggagaag gaggccatta tgcggttagg tgtgctcgat gtgggcagca acacagtcca
      721 tcttctcgtg gtggatgcgc accgtggtgg ccatcccacc ccgatgagtt ccaccaaggc
      781 cgcactgcgc ctcgccgagg ccatcgacgg ctccggcaaa ctcacgcgaa aaggcgccga
      841 caagctggtc gcgaccgtcg acgaattcgc caagatcgcc acgagttccg gctgtgccga
      901 gctcatggcc tttgccacct cggcggtgcg tgacgccacc aactccgaag acgtgctggc
      961 cagggtccgc gccgagaccg gggtgtccct gcaggtgctc agcggcgtcg acgaatcacg
     1021 gctgaccttc ctggcagtgc gccgctggta cggctggagc gcgggccgca tcatcaacat
     1081 cgacatcggc ggcggttcgc tggagctgtc caacggggtc gacgaggaac ccgaggtggc
     1141 gttgtcgctg ccgctgggcg cgggccggtt gaccagggag tggctgcccg acgatccacc
     1201 ggggcgcagg gctgtcgcga tgctgcgcga ctggctcgcc accgagctcg ccgacgcggg
     1261 cgccacgata cggcgcgccg gggctcctga cctggccgtg gcgacgtcga agacgttcag
     1321 gtcgctggcc cggctgaccg gagcggcacc gtcaggggcg ggcccccgcg tcaagcggac
     1381 gctcaccgcg accggattaa gacagctcat agctttcatc tctaggatga cggcggctga
     1441 ccgcgctgaa ctggaagggg tgagtgccga acgggctcca cagatcgtgg ccggtgcttt
     1501 ggttgctgaa gctagtatgc gagcactgga aatcgaatcc gtcgacattt gcccctgggc
     1561 gttgcgggaa gggctgatcc tgcggaagct cgacagcgag gccgatggaa ccgccctggt
     1621 ggccgcggct gcgcgcgacg ccggacgaaa gcttggtgcg gccgcgggca acacgcctgg
     1681 catcaatctg atcaaggagg acatgcgtgt caaggtgcat atggagggca acgtgaacgg
     1741 tcacgccttc gtgatcgagg gggagggtaa gggtaagcct tacgaaggga cccagaccgc
     1801 caacctcacc gtcaaggagg gcgcccctct cccattctcg tacgatatcc tcactaccgc
     1861 ggtgcattac gggaaccgcg tgttcaccaa gtaccccgag gatatccctg actacttcaa
     1921 gcagagcttc ccggagggat actcctggga gcggacgatg accttcgaag acaagggtat
     1981 ctgtacgatc cgcagcgaca tctcgctgga aggggactgt ttcttccaga acgtccgttt
     2041 caaggggacc aactttccgc cgaacggccc ggtcatgcag aagaagaccc tcaagtggga
     2101 gccgagcacg gagaagctgc acgttcggga cggcctcctg gtcggcaaca tcaacatggc
     2161 gctgctgctg gaaggcggcg gccactacct atgcgacttc aagaccacgt acaaagcgaa
     2221 gaaggtcgtc cagctgcccg acgcgcactt cgtggaccac cgcatcgaaa tcctggggaa
     2281 cgattcggac tacaacaagg tcaagttgta cgagcacgct gtggcacgct atagcccgct
     2341 cccgtcgcag gtctggttcg aaggcggcgg cggggagaac ctgtatttcc aggactacaa
     2401 ggacgacgac gacaagtgat ttaaataacc ccttggggcc tctaaacggg tcttgagggg
     2461 ttttttgtta actagcgtac gatcgaattc ctgcagctca cggtaactga tgccgtattt
     2521 gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc ctttgaatgg
     2581 gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcaccacc gactatttgc
     2641 aacagtgccg ttgatcgtgc tatgatcgac tgatgtcatc agcggtggag tgcaatgtcg
     2701 tgcaatacga atggcgaaaa gccgagctca tcggtcagct tctcaacctt ggggttaccc
     2761 ccggcggtgt gctgctggtc cacagctcct tccgtagcgt ccggcccctc gaagatgggc
     2821 cacttggact gatcgaggcc ctgcgtgcta cgctgggtcc gggagggacg ctcgtcatgc
     2881 cctcgtggtc aggtctggac gacgagccgt tcgatcctgc cacgtcgccc gttacaccgg
     2941 accttggagt tgtctctgac acattctggc gcctgccaaa tgtaaagcgc agcgcccatc
     3001 catttgcctt tgcggcagcg gggccacagg cagagcagat catctctgat ccattgcccc
     3061 tgccacctta ctcgcctgca agcccggtcg cccgtgtcca tgaactcgat gggcaggtac
     3121 ttctcctcgg cgtgggacac gatgccaaca cgacgctgca tcttgccgag ttgatggcaa
     3181 aggttcccta tggggtgccg agacactgca ccattcttca ggatggcaag ttggtacgcg
     3241 tcgattatct cgagaatgac cactgctgtg agcgctttgc cttggcggac aggtggctca
     3301 aggagaagag ccttcagaag gaaggtccag tcggtcatgc ctttgctcgg ttgatccgct
     3361 cccgcgacat tgtggcgaca gccctgggtc aactgggccg agatccgttg atcttcctgc
     3421 atccgccaga ggcgggatgc gaagaatgcg atgccgctcg ccagtcgatt ggctgagctc
     3481 atgagcggag aacgagatga cgttggaggg gcaaggtcgc gctgattgct ggggcaacac
     3541 gggggatcca ctagttccat gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3601 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3661 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3721 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     3781 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3841 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3901 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3961 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaggggag
     4021 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4081 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4141 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4201 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4261 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4321 cagccgaacg accgagcgca acgcgtgcgg ccgcggtacc cggggatcct ctagagtcga
     4381 ccaccaaggg caccatctct gcttgggcca ccccgttggc cgcagccagc tcgctgagag
     4441 ccgtgaacga cagggcgaac gccagcccgc cgacggcgag ggttccgacc gctgcaactc
     4501 ccggtgcaac cttgtcccgg tctattctct tcactgcacc agctccaatc tggtgtgaat
     4561 gcccctcgtc tgttcgcgca ggcggggggc tctattcgtt tgtcagcatc gaaagtagcc
     4621 agatcaggga tgcgttgcaa ccgcgtatgc ccaggtcaga agagtcgcac aagagttgca
     4681 gacccctgga aagaaaaatg gccagagggc gaaaacaccc tctgaccagc ggagcgggcg
     4741 acgggaatcg aacccgcgta gctagtttgg aagaatgggt gtctgccgac cacatatggg
     4801 ccggtcaaga taggttttta ccccctctcg gctgcatcct ctaagtggaa agaaattgca
     4861 ggtcgtagaa gcgcgttgaa gcctgagagt tgcacaggag ttgcaacccg gtagccttgt
     4921 tcacgacgag aggagaccta gttggcacgt cgcggatggg gatcgctgaa gactcagcgc
     4981 agcgggagga tccaagcctc atacgtcaac ccgcaggacg gtgtgaggta ctacgcgctg
     5041 cagacctacg acaacaagat ggacgccgaa gcctggctcg cgggcgagaa gcggctcatc
     5101 gagatggaga cctggacccc tccacaggac cgggcgaaga aggcagccgc cagcgccatc
     5161 acgctggagg agtacacccg gaagtggctc gtggagcgcg acctcgcaga cggcaccagg
     5221 gatctgtaca gcgggcacgc ggagcgccgc atctacccgg tgctaggtga agtggcggtc
     5281 acagagatga cgccagctct ggtgcgtgcg tggtgggccg ggatgggtag gaagcacccg
     5341 actgcccgcc ggcatgccta caacgtcctc cgggcggtga tgaacacagc ggtcgaggac
     5401 aagctgatcg cagagaaccc gtgccggatc gagcagaagg cagccgatga gcgcgacgta
     5461 gaggcgctga cgcctgagga gctggacatc gtcgccgctg agatcttcga gcactaccgg
     5521 atcgcggcat acatcctggc gtggacgagc ctccggttcg gagagctgat cgagcttcgc
     5581 cgcaaggaca tcgtggacga cggcatgacg atgaagctcc gggtgcgccg tggcgcttcc
     5641 cgcgtgggga acaagatcgt cgttggcaac gccaagaccg tccggtcgaa gcgtcctgtg
     5701 acggttccgc ctcacgtcgc ggagatgatc cgagcgcaca tgaaggaccg tacgaagatg
     5761 aacaagggcc ccgaggcatt cctggtgacc acgacgcagg gcaaccggct gtcgaagtcc
     5821 gcgttcacca agtcgctgaa gcgtggcta
//
