LOCUS       sequence-345651-        5834 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0953:Dendra
FEATURES             Location/Qualifiers
     source          1..5834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     336..355
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        336..354
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2068..2091
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2348..3148
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3273..3861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3762..3781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgtgcgga gacctgggca ccagccccgc cgccgccagg agcattgccg ttcccgccag
       61 aaatctagac ggtgaccaca acgcgcccgc tttgatcggg gacgtcgaaa tattggatcg
      121 tcggcaccgt cacggccgtg ggaggcggca cgatccgcga cgtgatgatc ggccgcatcc
      181 ccacggtgct gcgcagtgag ctctacgcca tcccggcgtt gatctgtgcg ttcgcacgca
      241 caggcccggt gtgagaaggg tctctgacga gcgggagaac tccctatcag tgatagagtt
      301 tgtcctccct atcagtgata gataggctta aggagtaata cgactcacta tagggagaag
      361 gaggccatta tggcaaacaa ccgcgattcg gtgatccggc tcggcacccg gggtagcctc
      421 ctggctacga cacaggccgg cactgtcagg gacgctctgc tggccgcagg gcatccctgt
      481 gagctcgtca tcatctccac cgagggtgac cgcaaccagg gaccgatcgc ggacatcggg
      541 atcggcgtgt tcaccgccgc tctgcgtgag gcgatccacg acggccgcgt cgacatggcc
      601 gtgcactcgt acaaggattt gcccaccgcg tccgacgaac gcttctgcat ccccgcgata
      661 ccgcgtcgtg aggacccacg cgacgcgctg gtggcccgcg acggactggt gctcggagag
      721 ttgccgacgg gctccgtgat cggcacgtcg agcccgcgac gggccgcaca gcttagagca
      781 ctgggtctcg gtttggaaat ccgcccccta cgaggcaacc tagataccag gttgaacagg
      841 gttacgagcg gtgaactcga cggcatcgtg gtcgcccggg cgggtctggc ccggatcgga
      901 cgactgaacg ttgtcaccga gacgctcgag ccggtgcaga tgttgccagc gccggctcag
      961 ggcgcgctcg cggtggaatg tcgtgcgggc gacaccgagc tgatcgcgat cttgtcggag
     1021 ttggacgacg ccgatacgcg agccgcggtc accgctgaac gggtcctgct cgccgaactg
     1081 gaggcgggtt gttccgcacc ggtcggtgcg atcgctgaag tggtcgagtc gatcgatgag
     1141 gacggcaacg tcttcgaaga gctgtcgttg cgcggctgcg cagcgacgct ggacggatcc
     1201 gacgtgatcc gtgcatccgg catcggtact cccgatcggg ccgccgaact cggcgtctcg
     1261 gtggctgcgg agctgttcga tctgggtgca cgggagctgt tggaagagcg tgggagtgag
     1321 aaaaagcttg gtgcggccgc gggcaacacg cctggcatca atctgatcaa ggaggacatg
     1381 cgtgtcaagg tgcatatgga gggcaacgtg aacggtcacg ccttcgtgat cgagggggag
     1441 ggtaagggta agccttacga agggacccag accgccaacc tcaccgtcaa ggagggcgcc
     1501 cctctcccat tctcgtacga tatcctcact accgcggtgc attacgggaa ccgcgtgttc
     1561 accaagtacc ccgaggatat ccctgactac ttcaagcaga gcttcccgga gggatactcc
     1621 tgggagcgga cgatgacctt cgaagacaag ggtatctgta cgatccgcag cgacatctcg
     1681 ctggaagggg actgtttctt ccagaacgtc cgtttcaagg ggaccaactt tccgccgaac
     1741 ggcccggtca tgcagaagaa gaccctcaag tgggagccga gcacggagaa gctgcacgtt
     1801 cgggacggcc tcctggtcgg caacatcaac atggcgctgc tgctggaagg cggcggccac
     1861 tacctatgcg acttcaagac cacgtacaaa gcgaagaagg tcgtccagct gcccgacgcg
     1921 cacttcgtgg accaccgcat cgaaatcctg gggaacgatt cggactacaa caaggtcaag
     1981 ttgtacgagc acgctgtggc acgctatagc ccgctcccgt cgcaggtctg gttcgaaggc
     2041 ggcggcgggg agaacctgta tttccaggac tacaaggacg acgacgacaa gtgatttaaa
     2101 taaccccttg gggcctctaa acgggtcttg aggggttttt tgttaactag cgtacgatcg
     2161 aattcctgca gctcacggta actgatgccg tatttgcagt accagcgtac ggcccacaga
     2221 atgatgtcac gctgaaaatg ccggcctttg aatgggttca tgtgcagctc catcagcaaa
     2281 aggggatgat aagtttatca ccaccgacta tttgcaacag tgccgttgat cgtgctatga
     2341 tcgactgatg tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc gaaaagccga
     2401 gctcatcggt cagcttctca accttggggt tacccccggc ggtgtgctgc tggtccacag
     2461 ctccttccgt agcgtccggc ccctcgaaga tgggccactt ggactgatcg aggccctgcg
     2521 tgctacgctg ggtccgggag ggacgctcgt catgccctcg tggtcaggtc tggacgacga
     2581 gccgttcgat cctgccacgt cgcccgttac accggacctt ggagttgtct ctgacacatt
     2641 ctggcgcctg ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg cagcggggcc
     2701 acaggcagag cagatcatct ctgatccatt gcccctgcca ccttactcgc ctgcaagccc
     2761 ggtcgcccgt gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg gacacgatgc
     2821 caacacgacg ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg tgccgagaca
     2881 ctgcaccatt cttcaggatg gcaagttggt acgcgtcgat tatctcgaga atgaccactg
     2941 ctgtgagcgc tttgccttgg cggacaggtg gctcaaggag aagagccttc agaaggaagg
     3001 tccagtcggt catgcctttg ctcggttgat ccgctcccgc gacattgtgg cgacagccct
     3061 gggtcaactg ggccgagatc cgttgatctt cctgcatccg ccagaggcgg gatgcgaaga
     3121 atgcgatgcc gctcgccagt cgattggctg agctcatgag cggagaacga gatgacgttg
     3181 gaggggcaag gtcgcgctga ttgctggggc aacacggggg atccactagt tccatgagcg
     3241 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3301 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3361 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     3421 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3481 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3541 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3601 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3661 gagctatgag aaagcgccac gcttcccgag gggagaaagg cggacaggta tccggtaagc
     3721 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3781 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3841 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3901 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3961 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcaacgcg
     4021 tgcggccgcg gtacccgggg atcctctaga gtcgaccacc aagggcacca tctctgcttg
     4081 ggccaccccg ttggccgcag ccagctcgct gagagccgtg aacgacaggg cgaacgccag
     4141 cccgccgacg gcgagggttc cgaccgctgc aactcccggt gcaaccttgt cccggtctat
     4201 tctcttcact gcaccagctc caatctggtg tgaatgcccc tcgtctgttc gcgcaggcgg
     4261 ggggctctat tcgtttgtca gcatcgaaag tagccagatc agggatgcgt tgcaaccgcg
     4321 tatgcccagg tcagaagagt cgcacaagag ttgcagaccc ctggaaagaa aaatggccag
     4381 agggcgaaaa caccctctga ccagcggagc gggcgacggg aatcgaaccc gcgtagctag
     4441 tttggaagaa tgggtgtctg ccgaccacat atgggccggt caagataggt ttttaccccc
     4501 tctcggctgc atcctctaag tggaaagaaa ttgcaggtcg tagaagcgcg ttgaagcctg
     4561 agagttgcac aggagttgca acccggtagc cttgttcacg acgagaggag acctagttgg
     4621 cacgtcgcgg atggggatcg ctgaagactc agcgcagcgg gaggatccaa gcctcatacg
     4681 tcaacccgca ggacggtgtg aggtactacg cgctgcagac ctacgacaac aagatggacg
     4741 ccgaagcctg gctcgcgggc gagaagcggc tcatcgagat ggagacctgg acccctccac
     4801 aggaccgggc gaagaaggca gccgccagcg ccatcacgct ggaggagtac acccggaagt
     4861 ggctcgtgga gcgcgacctc gcagacggca ccagggatct gtacagcggg cacgcggagc
     4921 gccgcatcta cccggtgcta ggtgaagtgg cggtcacaga gatgacgcca gctctggtgc
     4981 gtgcgtggtg ggccgggatg ggtaggaagc acccgactgc ccgccggcat gcctacaacg
     5041 tcctccgggc ggtgatgaac acagcggtcg aggacaagct gatcgcagag aacccgtgcc
     5101 ggatcgagca gaaggcagcc gatgagcgcg acgtagaggc gctgacgcct gaggagctgg
     5161 acatcgtcgc cgctgagatc ttcgagcact accggatcgc ggcatacatc ctggcgtgga
     5221 cgagcctccg gttcggagag ctgatcgagc ttcgccgcaa ggacatcgtg gacgacggca
     5281 tgacgatgaa gctccgggtg cgccgtggcg cttcccgcgt ggggaacaag atcgtcgttg
     5341 gcaacgccaa gaccgtccgg tcgaagcgtc ctgtgacggt tccgcctcac gtcgcggaga
     5401 tgatccgagc gcacatgaag gaccgtacga agatgaacaa gggccccgag gcattcctgg
     5461 tgaccacgac gcagggcaac cggctgtcga agtccgcgtt caccaagtcg ctgaagcgtg
     5521 gctacgccaa gatcggtcgg ccggaactcc gcatccacga cctccgcgct gtcggcgcta
     5581 cgttcgccgc tcaggcaggt gcgacgacca aggagctgat ggcccgtctc ggtcacacga
     5641 ctcctaggat ggcgatgaag taccagatgg cgtctgaggc ccgcgacgag gctatcgctg
     5701 aggcgatgtc caagctggcc aagacctcct gaaacgcaaa aagcccccct cccaaggaca
     5761 ctgagtccta aagagggggg tttcttgtca gtacgcgaag aaccacgcct ggccgcgagc
     5821 gccagcaccg ccgc
//
