LOCUS       sequence-345652-        6119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1041:Dendra
FEATURES             Location/Qualifiers
     source          1..6119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     695..714
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        695..713
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2712..2735
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2992..3792
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3917..4505
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4406..4425
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcaaccggct gtcgaagtcc gcgttcacca agtcgctgaa gcgtggctac gccaagatcg
       61 gtcggccgga actccgcatc cacgacctcc gcgctgtcgg cgctacgttc gccgctcagg
      121 caggtgcgac gaccaaggag ctgatggccc gtctcggtca cacgactcct aggatggcga
      181 tgaagtacca gatggcgtct gaggcccgcg acgaggctat cgctgaggcg atgtccaagc
      241 tggccaagac ctcctgaaac gcaaaaagcc cccctcccaa ggacactgag tcctaaagag
      301 gggggtttct tgtcagtacg cgaagaacca cgcctggccg cgagcgccag caccgccgct
      361 ctgtgcggag acctgggcac cagccccgcc gccgccagga gcattgccgt tcccgccaga
      421 aatctagacg gtgaccacaa cgcgcccgct ttgatcgggg acgtcgaaat attggatcgt
      481 cggcaccgtc acggccgtgg gaggcggcac gatccgcgac gtgatgatcg gccgcatccc
      541 cacggtgctg cgcagtgagc tctacgccat cccggcgttg atctgtgcgt tcgcacgcac
      601 aggcccggtg tgagaagggt ctctgacgag cgggagaact ccctatcagt gatagagttt
      661 gtcctcccta tcagtgatag ataggcttaa ggagtaatac gactcactat agggagaagg
      721 aggccattat gagctcggca cgcgagcgct catcgctact gatcaccgtc accggtgtcg
      781 accagccggg cgtcacctcg gcactgttcg aggtgctgtc ccggcacaag gtcgagctgc
      841 gcaacgtcga gcaggtcgtg atccgcggcc ggctcacgtt gggcgttctg gtggccgcgc
      901 cgtccgacgt ggccgacggc gatgatctgc gacgcgacgt cgaggccgcc atccacggcg
      961 tgggtctcga cgtcgcgatc gaacgcagcg acgacctgcc ggtgatgcgt gagccctcaa
     1021 cccacacgat cgtggtgctg ggccgtccga tcaccgccga ggcgttcggg gtggtcgccc
     1081 gcgcggtggc cgatctgggc gtcaacatcg atttcatccg cggcgtctcc gactacccgg
     1141 tgacggggct ggagctgcgc gtgtcggtcc cggcgggcgc ggcctacggg cagttgcagc
     1201 aggcgctcgc cacggtcgcc gcgaacgagg gcgtcgacat cgcgctggag gactacacgc
     1261 tgtcacgacg ggccaagcgg ctcatcgtgt tcgacgtcga ctcgaccctc atccagggtg
     1321 aggtgatcga gatgctggcc gagcacgccg gtgccgcggc agccgtcgcc gaggtgaccg
     1381 aggccgcgat gcggggtgaa ctcgacttcg ccgagtcttt gcaccgccgg gtcgcgacgc
     1441 tcgccgggct gcctgcctcg gtactcgacg aggtcgccga gcagttggag ctcacaccgg
     1501 gggcgcggac cacgatccgc accctccgca ggctgggcta ctactgcggt gtggtctcgg
     1561 gcggattccg ccaggtcatc gaaccgctcg cgcacgaact catgctcgac tacgtcgcgg
     1621 ccaacgaact ggagatcgtc gacggcaagc tcaccggacg cgtcgtcggc gacgtcgtgg
     1681 accggccggg caaggccaag gcgctgcggg acttcgccca gcaggtcggc gtaccgatgg
     1741 agcagaccgt cgccgtcggc gacggcgcca acgacatcga catgctgtcg gccgcgggcc
     1801 tcggggtggc gttcaacgcc aaacccgcgc tgcgtgaggt ggccgacgcg tcactgagcc
     1861 atccgtacct ggacaccgtg ctgttcatcc tgggcatcac gcgcggcgag atcgaggccg
     1921 ccgatgcgct cgacggtgtg gtgcgccgcg tcgagatccc ggattataag cttggtgcgg
     1981 ccgcgggcaa cacgcctggc atcaatctga tcaaggagga catgcgtgtc aaggtgcata
     2041 tggagggcaa cgtgaacggt cacgccttcg tgatcgaggg ggagggtaag ggtaagcctt
     2101 acgaagggac ccagaccgcc aacctcaccg tcaaggaggg cgcccctctc ccattctcgt
     2161 acgatatcct cactaccgcg gtgcattacg ggaaccgcgt gttcaccaag taccccgagg
     2221 atatccctga ctacttcaag cagagcttcc cggagggata ctcctgggag cggacgatga
     2281 ccttcgaaga caagggtatc tgtacgatcc gcagcgacat ctcgctggaa ggggactgtt
     2341 tcttccagaa cgtccgtttc aaggggacca actttccgcc gaacggcccg gtcatgcaga
     2401 agaagaccct caagtgggag ccgagcacgg agaagctgca cgttcgggac ggcctcctgg
     2461 tcggcaacat caacatggcg ctgctgctgg aaggcggcgg ccactaccta tgcgacttca
     2521 agaccacgta caaagcgaag aaggtcgtcc agctgcccga cgcgcacttc gtggaccacc
     2581 gcatcgaaat cctggggaac gattcggact acaacaaggt caagttgtac gagcacgctg
     2641 tggcacgcta tagcccgctc ccgtcgcagg tctggttcga aggcggcggc ggggagaacc
     2701 tgtatttcca ggactacaag gacgacgacg acaagtgatt taaataaccc cttggggcct
     2761 ctaaacgggt cttgaggggt tttttgttaa ctagcgtacg atcgaattcc tgcagctcac
     2821 ggtaactgat gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa
     2881 aatgccggcc tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt
     2941 atcaccaccg actatttgca acagtgccgt tgatcgtgct atgatcgact gatgtcatca
     3001 gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat cggtcagctt
     3061 ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc acagctcctt ccgtagcgtc
     3121 cggcccctcg aagatgggcc acttggactg atcgaggccc tgcgtgctac gctgggtccg
     3181 ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt cgatcctgcc
     3241 acgtcgcccg ttacaccgga ccttggagtt gtctctgaca cattctggcg cctgccaaat
     3301 gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg ggccacaggc agagcagatc
     3361 atctctgatc cattgcccct gccaccttac tcgcctgcaa gcccggtcgc ccgtgtccat
     3421 gaactcgatg ggcaggtact tctcctcggc gtgggacacg atgccaacac gacgctgcat
     3481 cttgccgagt tgatggcaaa ggttccctat ggggtgccga gacactgcac cattcttcag
     3541 gatggcaagt tggtacgcgt cgattatctc gagaatgacc actgctgtga gcgctttgcc
     3601 ttggcggaca ggtggctcaa ggagaagagc cttcagaagg aaggtccagt cggtcatgcc
     3661 tttgctcggt tgatccgctc ccgcgacatt gtggcgacag ccctgggtca actgggccga
     3721 gatccgttga tcttcctgca tccgccagag gcgggatgcg aagaatgcga tgccgctcgc
     3781 cagtcgattg gctgagctca tgagcggaga acgagatgac gttggagggg caaggtcgcg
     3841 ctgattgctg gggcaacacg ggggatccac tagttccatg agcgtcagac cccgtagaaa
     3901 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3961 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4021 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     4081 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4141 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4201 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4261 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4321 ccacgcttcc cgaggggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4381 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4441 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4501 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4561 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4621 gagctgatac cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc cgcggtaccc
     4681 ggggatcctc tagagtcgac caccaagggc accatctctg cttgggccac cccgttggcc
     4741 gcagccagct cgctgagagc cgtgaacgac agggcgaacg ccagcccgcc gacggcgagg
     4801 gttccgaccg ctgcaactcc cggtgcaacc ttgtcccggt ctattctctt cactgcacca
     4861 gctccaatct ggtgtgaatg cccctcgtct gttcgcgcag gcggggggct ctattcgttt
     4921 gtcagcatcg aaagtagcca gatcagggat gcgttgcaac cgcgtatgcc caggtcagaa
     4981 gagtcgcaca agagttgcag acccctggaa agaaaaatgg ccagagggcg aaaacaccct
     5041 ctgaccagcg gagcgggcga cgggaatcga acccgcgtag ctagtttgga agaatgggtg
     5101 tctgccgacc acatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc
     5161 taagtggaaa gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt
     5221 tgcaacccgg tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg
     5281 atcgctgaag actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg
     5341 tgtgaggtac tacgcgctgc agacctacga caacaagatg gacgccgaag cctggctcgc
     5401 gggcgagaag cggctcatcg agatggagac ctggacccct ccacaggacc gggcgaagaa
     5461 ggcagccgcc agcgccatca cgctggagga gtacacccgg aagtggctcg tggagcgcga
     5521 cctcgcagac ggcaccaggg atctgtacag cgggcacgcg gagcgccgca tctacccggt
     5581 gctaggtgaa gtggcggtca cagagatgac gccagctctg gtgcgtgcgt ggtgggccgg
     5641 gatgggtagg aagcacccga ctgcccgccg gcatgcctac aacgtcctcc gggcggtgat
     5701 gaacacagcg gtcgaggaca agctgatcgc agagaacccg tgccggatcg agcagaaggc
     5761 agccgatgag cgcgacgtag aggcgctgac gcctgaggag ctggacatcg tcgccgctga
     5821 gatcttcgag cactaccgga tcgcggcata catcctggcg tggacgagcc tccggttcgg
     5881 agagctgatc gagcttcgcc gcaaggacat cgtggacgac ggcatgacga tgaagctccg
     5941 ggtgcgccgt ggcgcttccc gcgtggggaa caagatcgtc gttggcaacg ccaagaccgt
     6001 ccggtcgaag cgtcctgtga cggttccgcc tcacgtcgcg gagatgatcc gagcgcacat
     6061 gaaggaccgt acgaagatga acaagggccc cgaggcattc ctggtgacca cgacgcagg
//
