LOCUS       sequence-345653-        5201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1063:Dendra
FEATURES             Location/Qualifiers
     source          1..5201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1275..1294
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1275..1293
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2374..2397
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2654..3454
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3579..4167
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4068..4087
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatctgtaca gcgggcacgc ggagcgccgc atctacccgg tgctaggtga agtggcggtc
       61 acagagatga cgccagctct ggtgcgtgcg tggtgggccg ggatgggtag gaagcacccg
      121 actgcccgcc ggcatgccta caacgtcctc cgggcggtga tgaacacagc ggtcgaggac
      181 aagctgatcg cagagaaccc gtgccggatc gagcagaagg cagccgatga gcgcgacgta
      241 gaggcgctga cgcctgagga gctggacatc gtcgccgctg agatcttcga gcactaccgg
      301 atcgcggcat acatcctggc gtggacgagc ctccggttcg gagagctgat cgagcttcgc
      361 cgcaaggaca tcgtggacga cggcatgacg atgaagctcc gggtgcgccg tggcgcttcc
      421 cgcgtgggga acaagatcgt cgttggcaac gccaagaccg tccggtcgaa gcgtcctgtg
      481 acggttccgc ctcacgtcgc ggagatgatc cgagcgcaca tgaaggaccg tacgaagatg
      541 aacaagggcc ccgaggcatt cctggtgacc acgacgcagg gcaaccggct gtcgaagtcc
      601 gcgttcacca agtcgctgaa gcgtggctac gccaagatcg gtcggccgga actccgcatc
      661 cacgacctcc gcgctgtcgg cgctacgttc gccgctcagg caggtgcgac gaccaaggag
      721 ctgatggccc gtctcggtca cacgactcct aggatggcga tgaagtacca gatggcgtct
      781 gaggcccgcg acgaggctat cgctgaggcg atgtccaagc tggccaagac ctcctgaaac
      841 gcaaaaagcc cccctcccaa ggacactgag tcctaaagag gggggtttct tgtcagtacg
      901 cgaagaacca cgcctggccg cgagcgccag caccgccgct ctgtgcggag acctgggcac
      961 cagccccgcc gccgccagga gcattgccgt tcccgccaga aatctagacg gtgaccacaa
     1021 cgcgcccgct ttgatcgggg acgtcgaaat attggatcgt cggcaccgtc acggccgtgg
     1081 gaggcggcac gatccgcgac gtgatgatcg gccgcatccc cacggtgctg cgcagtgagc
     1141 tctacgccat cccggcgttg atctgtgcgt tcgcacgcac aggcccggtg tgagaagggt
     1201 ctctgacgag cgggagaact ccctatcagt gatagagttt gtcctcccta tcagtgatag
     1261 ataggcttaa ggagtaatac gactcactat agggagaagg aggccattat gaacgcattt
     1321 ctcgccaaga tcgcggcctg gctcaacgcc gggtaccccg agggcgtgcc cggccccgac
     1381 cgggtgccgc tgctggcatt gttgacccgt cggctcacca acgacgagat caaggccatc
     1441 gcggaggatc tggagaaacg tgctcacttc gaccacatcg acatcggcgt gctgataacc
     1501 cagatgaccg acgagatgcc acgcgaagag gacatcgagc gtgtgcgcag gcatctcgcg
     1561 ttgcagggct ggccgctcga cgatccgcgc gacggcgagg agcccgacgg tgaatcggga
     1621 ggcccgcgaa agcttggtgc ggccgcgggc aacacgcctg gcatcaatct gatcaaggag
     1681 gacatgcgtg tcaaggtgca tatggagggc aacgtgaacg gtcacgcctt cgtgatcgag
     1741 ggggagggta agggtaagcc ttacgaaggg acccagaccg ccaacctcac cgtcaaggag
     1801 ggcgcccctc tcccattctc gtacgatatc ctcactaccg cggtgcatta cgggaaccgc
     1861 gtgttcacca agtaccccga ggatatccct gactacttca agcagagctt cccggaggga
     1921 tactcctggg agcggacgat gaccttcgaa gacaagggta tctgtacgat ccgcagcgac
     1981 atctcgctgg aaggggactg tttcttccag aacgtccgtt tcaaggggac caactttccg
     2041 ccgaacggcc cggtcatgca gaagaagacc ctcaagtggg agccgagcac ggagaagctg
     2101 cacgttcggg acggcctcct ggtcggcaac atcaacatgg cgctgctgct ggaaggcggc
     2161 ggccactacc tatgcgactt caagaccacg tacaaagcga agaaggtcgt ccagctgccc
     2221 gacgcgcact tcgtggacca ccgcatcgaa atcctgggga acgattcgga ctacaacaag
     2281 gtcaagttgt acgagcacgc tgtggcacgc tatagcccgc tcccgtcgca ggtctggttc
     2341 gaaggcggcg gcggggagaa cctgtatttc caggactaca aggacgacga cgacaagtga
     2401 tttaaataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt aactagcgta
     2461 cgatcgaatt cctgcagctc acggtaactg atgccgtatt tgcagtacca gcgtacggcc
     2521 cacagaatga tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg cagctccatc
     2581 agcaaaaggg gatgataagt ttatcaccac cgactatttg caacagtgcc gttgatcgtg
     2641 ctatgatcga ctgatgtcat cagcggtgga gtgcaatgtc gtgcaatacg aatggcgaaa
     2701 agccgagctc atcggtcagc ttctcaacct tggggttacc cccggcggtg tgctgctggt
     2761 ccacagctcc ttccgtagcg tccggcccct cgaagatggg ccacttggac tgatcgaggc
     2821 cctgcgtgct acgctgggtc cgggagggac gctcgtcatg ccctcgtggt caggtctgga
     2881 cgacgagccg ttcgatcctg ccacgtcgcc cgttacaccg gaccttggag ttgtctctga
     2941 cacattctgg cgcctgccaa atgtaaagcg cagcgcccat ccatttgcct ttgcggcagc
     3001 ggggccacag gcagagcaga tcatctctga tccattgccc ctgccacctt actcgcctgc
     3061 aagcccggtc gcccgtgtcc atgaactcga tgggcaggta cttctcctcg gcgtgggaca
     3121 cgatgccaac acgacgctgc atcttgccga gttgatggca aaggttccct atggggtgcc
     3181 gagacactgc accattcttc aggatggcaa gttggtacgc gtcgattatc tcgagaatga
     3241 ccactgctgt gagcgctttg ccttggcgga caggtggctc aaggagaaga gccttcagaa
     3301 ggaaggtcca gtcggtcatg cctttgctcg gttgatccgc tcccgcgaca ttgtggcgac
     3361 agccctgggt caactgggcc gagatccgtt gatcttcctg catccgccag aggcgggatg
     3421 cgaagaatgc gatgccgctc gccagtcgat tggctgagct catgagcgga gaacgagatg
     3481 acgttggagg ggcaaggtcg cgctgattgc tggggcaaca cgggggatcc actagttcca
     3541 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3601 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3661 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3721 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3781 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3841 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3901 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3961 cagcgtgagc tatgagaaag cgccacgctt cccgagggga gaaaggcgga caggtatccg
     4021 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4081 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4141 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4201 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4261 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     4321 aacgcgtgcg gccgcggtac ccggggatcc tctagagtcg accaccaagg gcaccatctc
     4381 tgcttgggcc accccgttgg ccgcagccag ctcgctgaga gccgtgaacg acagggcgaa
     4441 cgccagcccg ccgacggcga gggttccgac cgctgcaact cccggtgcaa ccttgtcccg
     4501 gtctattctc ttcactgcac cagctccaat ctggtgtgaa tgcccctcgt ctgttcgcgc
     4561 aggcgggggg ctctattcgt ttgtcagcat cgaaagtagc cagatcaggg atgcgttgca
     4621 accgcgtatg cccaggtcag aagagtcgca caagagttgc agacccctgg aaagaaaaat
     4681 ggccagaggg cgaaaacacc ctctgaccag cggagcgggc gacgggaatc gaacccgcgt
     4741 agctagtttg gaagaatggg tgtctgccga ccacatatgg gccggtcaag ataggttttt
     4801 accccctctc ggctgcatcc tctaagtgga aagaaattgc aggtcgtaga agcgcgttga
     4861 agcctgagag ttgcacagga gttgcaaccc ggtagccttg ttcacgacga gaggagacct
     4921 agttggcacg tcgcggatgg ggatcgctga agactcagcg cagcgggagg atccaagcct
     4981 catacgtcaa cccgcaggac ggtgtgaggt actacgcgct gcagacctac gacaacaaga
     5041 tggacgccga agcctggctc gcgggcgaga agcggctcat cgagatggag acctggaccc
     5101 ctccacagga ccgggcgaag aaggcagccg ccagcgccat cacgctggag gagtacaccc
     5161 ggaagtggct cgtggagcgc gacctcgcag acggcaccag g
//
