LOCUS       sequence-345660-        5678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0924:Dendra
FEATURES             Location/Qualifiers
     source          1..5678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     342..361
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        342..360
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1918..1941
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2198..2998
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3123..3711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3612..3631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgccgctctg tgcggagacc tgggcaccag ccccgccgcc gccaggagca ttgccgttcc
       61 cgccagaaat ctagacggtg accacaacgc gcccgctttg atcggggacg tcgaaatatt
      121 ggatcgtcgg caccgtcacg gccgtgggag gcggcacgat ccgcgacgtg atgatcggcc
      181 gcatccccac ggtgctgcgc agtgagctct acgccatccc ggcgttgatc tgtgcgttcg
      241 cacgcacagg cccggtgtga gaagggtctc tgacgagcgg gagaactccc tatcagtgat
      301 agagtttgtc ctccctatca gtgatagata ggcttaagga gtaatacgac tcactatagg
      361 gagaaggagg ccattatgat gcggatcgct ctcgcccaga tcaccaccgg tacggacccg
      421 tcgtcgaatc tggagctggt cgagtcctgc acccggcgcg ctgccgacga cggcgcgcga
      481 ctggtgctgt tccccgaggc cacgatgtgc cggttcgggg tgccgctggg cccggtggcc
      541 caaccgctcg acgggccgtg ggcgcgcgcc gtgcgcgaca tcgcagcgcg tgccggtgtg
      601 gtggtggtcg cgggcatgtt ctcgccgagc gacgacggcc gcgtcaccaa cacgctgatc
      661 gccgcgggcg cgggcgtgga cacccactac cacaagatcc acctgtacga cgcgttcggt
      721 ttcaccgaat cacgcaccgt cgcaccgggt ttcacgccga cgacgatcac cgtcgacggc
      781 acgccggtcg gcatcaccac gtgttacgac atccgtttcc ccgagttgta cgtcgagctc
      841 gcgcgtcgcg gtgcccagct gatcacggtc cacgcgtcgt gggggtccgg gccaggcaag
      901 ctggatcagt ggacgctgct cgcgcgcgcc agggcactcg acacgaccgg ttacatcgcc
      961 gcggtcgatc aggcctatcc cggtgacgag atcgccaagg ccggccccac gggtgtcggc
     1021 ggaagcctgg tcgcctcccc caccggggat gtgctggcct ccgcgggcgc cgaacccgag
     1081 ctgctgaccg tcgacatcga cctcgacgcc gcgacgcgcg tcagggacac cgtcgccatt
     1141 ctggacaacc ggtcggcgct ggcggggctc agcaagcttg gtgcggccgc gggcaacacg
     1201 cctggcatca atctgatcaa ggaggacatg cgtgtcaagg tgcatatgga gggcaacgtg
     1261 aacggtcacg ccttcgtgat cgagggggag ggtaagggta agccttacga agggacccag
     1321 accgccaacc tcaccgtcaa ggagggcgcc cctctcccat tctcgtacga tatcctcact
     1381 accgcggtgc attacgggaa ccgcgtgttc accaagtacc ccgaggatat ccctgactac
     1441 ttcaagcaga gcttcccgga gggatactcc tgggagcgga cgatgacctt cgaagacaag
     1501 ggtatctgta cgatccgcag cgacatctcg ctggaagggg actgtttctt ccagaacgtc
     1561 cgtttcaagg ggaccaactt tccgccgaac ggcccggtca tgcagaagaa gaccctcaag
     1621 tgggagccga gcacggagaa gctgcacgtt cgggacggcc tcctggtcgg caacatcaac
     1681 atggcgctgc tgctggaagg cggcggccac tacctatgcg acttcaagac cacgtacaaa
     1741 gcgaagaagg tcgtccagct gcccgacgcg cacttcgtgg accaccgcat cgaaatcctg
     1801 gggaacgatt cggactacaa caaggtcaag ttgtacgagc acgctgtggc acgctatagc
     1861 ccgctcccgt cgcaggtctg gttcgaaggc ggcggcgggg agaacctgta tttccaggac
     1921 tacaaggacg acgacgacaa gtgatttaaa taaccccttg gggcctctaa acgggtcttg
     1981 aggggttttt tgttaactag cgtacgatcg aattcctgca gctcacggta actgatgccg
     2041 tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg ccggcctttg
     2101 aatgggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca ccaccgacta
     2161 tttgcaacag tgccgttgat cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa
     2221 tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt cagcttctca accttggggt
     2281 tacccccggc ggtgtgctgc tggtccacag ctccttccgt agcgtccggc ccctcgaaga
     2341 tgggccactt ggactgatcg aggccctgcg tgctacgctg ggtccgggag ggacgctcgt
     2401 catgccctcg tggtcaggtc tggacgacga gccgttcgat cctgccacgt cgcccgttac
     2461 accggacctt ggagttgtct ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc
     2521 ccatccattt gcctttgcgg cagcggggcc acaggcagag cagatcatct ctgatccatt
     2581 gcccctgcca ccttactcgc ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca
     2641 ggtacttctc ctcggcgtgg gacacgatgc caacacgacg ctgcatcttg ccgagttgat
     2701 ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt cttcaggatg gcaagttggt
     2761 acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc tttgccttgg cggacaggtg
     2821 gctcaaggag aagagccttc agaaggaagg tccagtcggt catgcctttg ctcggttgat
     2881 ccgctcccgc gacattgtgg cgacagccct gggtcaactg ggccgagatc cgttgatctt
     2941 cctgcatccg ccagaggcgg gatgcgaaga atgcgatgcc gctcgccagt cgattggctg
     3001 agctcatgag cggagaacga gatgacgttg gaggggcaag gtcgcgctga ttgctggggc
     3061 aacacggggg atccactagt tccatgagcg tcagaccccg tagaaaagat caaaggatct
     3121 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3181 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3241 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     3301 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3361 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3421 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3481 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgag
     3541 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3601 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3661 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3721 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3781 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3841 cgccgcagcc gaacgaccga gcgcaacgcg tgcggccgcg gtacccgggg atcctctaga
     3901 gtcgaccacc aagggcacca tctctgcttg ggccaccccg ttggccgcag ccagctcgct
     3961 gagagccgtg aacgacaggg cgaacgccag cccgccgacg gcgagggttc cgaccgctgc
     4021 aactcccggt gcaaccttgt cccggtctat tctcttcact gcaccagctc caatctggtg
     4081 tgaatgcccc tcgtctgttc gcgcaggcgg ggggctctat tcgtttgtca gcatcgaaag
     4141 tagccagatc agggatgcgt tgcaaccgcg tatgcccagg tcagaagagt cgcacaagag
     4201 ttgcagaccc ctggaaagaa aaatggccag agggcgaaaa caccctctga ccagcggagc
     4261 gggcgacggg aatcgaaccc gcgtagctag tttggaagaa tgggtgtctg ccgaccacat
     4321 atgggccggt caagataggt ttttaccccc tctcggctgc atcctctaag tggaaagaaa
     4381 ttgcaggtcg tagaagcgcg ttgaagcctg agagttgcac aggagttgca acccggtagc
     4441 cttgttcacg acgagaggag acctagttgg cacgtcgcgg atggggatcg ctgaagactc
     4501 agcgcagcgg gaggatccaa gcctcatacg tcaacccgca ggacggtgtg aggtactacg
     4561 cgctgcagac ctacgacaac aagatggacg ccgaagcctg gctcgcgggc gagaagcggc
     4621 tcatcgagat ggagacctgg acccctccac aggaccgggc gaagaaggca gccgccagcg
     4681 ccatcacgct ggaggagtac acccggaagt ggctcgtgga gcgcgacctc gcagacggca
     4741 ccagggatct gtacagcggg cacgcggagc gccgcatcta cccggtgcta ggtgaagtgg
     4801 cggtcacaga gatgacgcca gctctggtgc gtgcgtggtg ggccgggatg ggtaggaagc
     4861 acccgactgc ccgccggcat gcctacaacg tcctccgggc ggtgatgaac acagcggtcg
     4921 aggacaagct gatcgcagag aacccgtgcc ggatcgagca gaaggcagcc gatgagcgcg
     4981 acgtagaggc gctgacgcct gaggagctgg acatcgtcgc cgctgagatc ttcgagcact
     5041 accggatcgc ggcatacatc ctggcgtgga cgagcctccg gttcggagag ctgatcgagc
     5101 ttcgccgcaa ggacatcgtg gacgacggca tgacgatgaa gctccgggtg cgccgtggcg
     5161 cttcccgcgt ggggaacaag atcgtcgttg gcaacgccaa gaccgtccgg tcgaagcgtc
     5221 ctgtgacggt tccgcctcac gtcgcggaga tgatccgagc gcacatgaag gaccgtacga
     5281 agatgaacaa gggccccgag gcattcctgg tgaccacgac gcagggcaac cggctgtcga
     5341 agtccgcgtt caccaagtcg ctgaagcgtg gctacgccaa gatcggtcgg ccggaactcc
     5401 gcatccacga cctccgcgct gtcggcgcta cgttcgccgc tcaggcaggt gcgacgacca
     5461 aggagctgat ggcccgtctc ggtcacacga ctcctaggat ggcgatgaag taccagatgg
     5521 cgtctgaggc ccgcgacgag gctatcgctg aggcgatgtc caagctggcc aagacctcct
     5581 gaaacgcaaa aagcccccct cccaaggaca ctgagtccta aagagggggg tttcttgtca
     5641 gtacgcgaag aaccacgcct ggccgcgagc gccagcac
//
