LOCUS       sequence-345664-        6299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_1353:Dendra
FEATURES             Location/Qualifiers
     source          1..6299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     705..724
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        705..723
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2902..2925
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3182..3982
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      4107..4695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4596..4615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgacgcagg gcaaccggct gtcgaagtcc gcgttcacca agtcgctgaa gcgtggctac
       61 gccaagatcg gtcggccgga actccgcatc cacgacctcc gcgctgtcgg cgctacgttc
      121 gccgctcagg caggtgcgac gaccaaggag ctgatggccc gtctcggtca cacgactcct
      181 aggatggcga tgaagtacca gatggcgtct gaggcccgcg acgaggctat cgctgaggcg
      241 atgtccaagc tggccaagac ctcctgaaac gcaaaaagcc cccctcccaa ggacactgag
      301 tcctaaagag gggggtttct tgtcagtacg cgaagaacca cgcctggccg cgagcgccag
      361 caccgccgct ctgtgcggag acctgggcac cagccccgcc gccgccagga gcattgccgt
      421 tcccgccaga aatctagacg gtgaccacaa cgcgcccgct ttgatcgggg acgtcgaaat
      481 attggatcgt cggcaccgtc acggccgtgg gaggcggcac gatccgcgac gtgatgatcg
      541 gccgcatccc cacggtgctg cgcagtgagc tctacgccat cccggcgttg atctgtgcgt
      601 tcgcacgcac aggcccggtg tgagaagggt ctctgacgag cgggagaact ccctatcagt
      661 gatagagttt gtcctcccta tcagtgatag ataggcttaa ggagtaatac gactcactat
      721 agggagaagg aggccattat gagcactacg ccggcgaaaa ccaagcatcg cgaggtggcc
      781 aggctggacc gcgtgccgct cccggccgag gccgcgcgca tcggcgcgac cggatggcag
      841 atcacccgca ccggggcccg cgtcgcggcc aacatcttcg gccgcggatc tcttcagcag
      901 aagatcgtca agcaggttcc caagaccttc tccgacctgg gacccaccta cgtcaagttc
      961 gggcagatca tcgcgtcgag ccccggcgcc ttcggcgaac cgctgagccg cgagttccgc
     1021 ggcctgctgg atcgggtgcc tccggcgaac ccggacgagg tgcagaagct gatccgcgag
     1081 gaactcggtg acgatccgca caagctgttc aagacgttcg acgacacgcc gttcgcctcg
     1141 gcctccatcg cgcaggtcca ctacgccacg ctgcacagcg gcgaggaggt cgtcgtcaag
     1201 atccagcgcc cgggcatccg gcgccgcgtg gccgccgacc tgcagatcct caagcgcggg
     1261 gcacgtctgg tcgaattcgc caagctgggt cagcggttga gcgcgcagga cgtcgtcgcc
     1321 gacttcgccg acaacctcgc cgaggaactc gacttccggc tcgaggccca gtcgatggac
     1381 gcctgggtgg cgcacatgca cgcctcgccg ctgggcgcca acatccgcgt ccccacggtg
     1441 tactgggacc tgaccagcga gcgcgtgctg accatggaac gcatcaccgg tgtgcgcatc
     1501 gacgacgtgg ccgcgatccg caagaagggt ttcgacggca ccgaactggt caaggccctg
     1561 ctgttcagcg tgttcgaggg cggcctgcgg cacggcctgt tccacggcga cctgcacgcg
     1621 ggcaacctct acgtcgacga cgacggcaag atcgtgttct tcgacttcgg catcatgggc
     1681 cgcatcgatc cgcgcacgcg ctggctgctg cgtgaactgg tgcacgccct gctggtcaag
     1741 aaggaccacg ccgcggcggg caagatcgtg gtgctgatgg gcgcggtcgg caccgtcaaa
     1801 cccgagggcc aggccgccaa ggacctcgag gcgttcgcca cgccgctgac catgaagtcg
     1861 ctcggcgaca tgtcctacgc cgagatcggc aggcagctct cggccctggc cgacgcctac
     1921 gacgtcaagc tgccccgcga gctcgtgctg atcggcaagc agttcctcta cgtcgagcgc
     1981 tacatgaagt tgctcgcgcc caagtggcag atgatgagcg atccgcagct caccggttac
     2041 ttcgccaact tcatggtcga ggtgagccgg gagcacaagg agatcgacga cgtcgacgag
     2101 atcccagaca tccccgagac cggaaccgag acgcaggcgc ccagcgggga caaggccaag
     2161 cttggtgcgg ccgcgggcaa cacgcctggc atcaatctga tcaaggagga catgcgtgtc
     2221 aaggtgcata tggagggcaa cgtgaacggt cacgccttcg tgatcgaggg ggagggtaag
     2281 ggtaagcctt acgaagggac ccagaccgcc aacctcaccg tcaaggaggg cgcccctctc
     2341 ccattctcgt acgatatcct cactaccgcg gtgcattacg ggaaccgcgt gttcaccaag
     2401 taccccgagg atatccctga ctacttcaag cagagcttcc cggagggata ctcctgggag
     2461 cggacgatga ccttcgaaga caagggtatc tgtacgatcc gcagcgacat ctcgctggaa
     2521 ggggactgtt tcttccagaa cgtccgtttc aaggggacca actttccgcc gaacggcccg
     2581 gtcatgcaga agaagaccct caagtgggag ccgagcacgg agaagctgca cgttcgggac
     2641 ggcctcctgg tcggcaacat caacatggcg ctgctgctgg aaggcggcgg ccactaccta
     2701 tgcgacttca agaccacgta caaagcgaag aaggtcgtcc agctgcccga cgcgcacttc
     2761 gtggaccacc gcatcgaaat cctggggaac gattcggact acaacaaggt caagttgtac
     2821 gagcacgctg tggcacgcta tagcccgctc ccgtcgcagg tctggttcga aggcggcggc
     2881 ggggagaacc tgtatttcca ggactacaag gacgacgacg acaagtgatt taaataaccc
     2941 cttggggcct ctaaacgggt cttgaggggt tttttgttaa ctagcgtacg atcgaattcc
     3001 tgcagctcac ggtaactgat gccgtatttg cagtaccagc gtacggccca cagaatgatg
     3061 tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca gctccatcag caaaagggga
     3121 tgataagttt atcaccaccg actatttgca acagtgccgt tgatcgtgct atgatcgact
     3181 gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat
     3241 cggtcagctt ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc acagctcctt
     3301 ccgtagcgtc cggcccctcg aagatgggcc acttggactg atcgaggccc tgcgtgctac
     3361 gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt
     3421 cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt gtctctgaca cattctggcg
     3481 cctgccaaat gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg ggccacaggc
     3541 agagcagatc atctctgatc cattgcccct gccaccttac tcgcctgcaa gcccggtcgc
     3601 ccgtgtccat gaactcgatg ggcaggtact tctcctcggc gtgggacacg atgccaacac
     3661 gacgctgcat cttgccgagt tgatggcaaa ggttccctat ggggtgccga gacactgcac
     3721 cattcttcag gatggcaagt tggtacgcgt cgattatctc gagaatgacc actgctgtga
     3781 gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc cttcagaagg aaggtccagt
     3841 cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt gtggcgacag ccctgggtca
     3901 actgggccga gatccgttga tcttcctgca tccgccagag gcgggatgcg aagaatgcga
     3961 tgccgctcgc cagtcgattg gctgagctca tgagcggaga acgagatgac gttggagggg
     4021 caaggtcgcg ctgattgctg gggcaacacg ggggatccac tagttccatg agcgtcagac
     4081 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4141 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4201 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     4261 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4321 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4381 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4441 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4501 tgagaaagcg ccacgcttcc cgaggggaga aaggcggaca ggtatccggt aagcggcagg
     4561 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4621 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4681 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4741 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4801 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc
     4861 cgcggtaccc ggggatcctc tagagtcgac caccaagggc accatctctg cttgggccac
     4921 cccgttggcc gcagccagct cgctgagagc cgtgaacgac agggcgaacg ccagcccgcc
     4981 gacggcgagg gttccgaccg ctgcaactcc cggtgcaacc ttgtcccggt ctattctctt
     5041 cactgcacca gctccaatct ggtgtgaatg cccctcgtct gttcgcgcag gcggggggct
     5101 ctattcgttt gtcagcatcg aaagtagcca gatcagggat gcgttgcaac cgcgtatgcc
     5161 caggtcagaa gagtcgcaca agagttgcag acccctggaa agaaaaatgg ccagagggcg
     5221 aaaacaccct ctgaccagcg gagcgggcga cgggaatcga acccgcgtag ctagtttgga
     5281 agaatgggtg tctgccgacc acatatgggc cggtcaagat aggtttttac cccctctcgg
     5341 ctgcatcctc taagtggaaa gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt
     5401 gcacaggagt tgcaacccgg tagccttgtt cacgacgaga ggagacctag ttggcacgtc
     5461 gcggatgggg atcgctgaag actcagcgca gcgggaggat ccaagcctca tacgtcaacc
     5521 cgcaggacgg tgtgaggtac tacgcgctgc agacctacga caacaagatg gacgccgaag
     5581 cctggctcgc gggcgagaag cggctcatcg agatggagac ctggacccct ccacaggacc
     5641 gggcgaagaa ggcagccgcc agcgccatca cgctggagga gtacacccgg aagtggctcg
     5701 tggagcgcga cctcgcagac ggcaccaggg atctgtacag cgggcacgcg gagcgccgca
     5761 tctacccggt gctaggtgaa gtggcggtca cagagatgac gccagctctg gtgcgtgcgt
     5821 ggtgggccgg gatgggtagg aagcacccga ctgcccgccg gcatgcctac aacgtcctcc
     5881 gggcggtgat gaacacagcg gtcgaggaca agctgatcgc agagaacccg tgccggatcg
     5941 agcagaaggc agccgatgag cgcgacgtag aggcgctgac gcctgaggag ctggacatcg
     6001 tcgccgctga gatcttcgag cactaccgga tcgcggcata catcctggcg tggacgagcc
     6061 tccggttcgg agagctgatc gagcttcgcc gcaaggacat cgtggacgac ggcatgacga
     6121 tgaagctccg ggtgcgccgt ggcgcttccc gcgtggggaa caagatcgtc gttggcaacg
     6181 ccaagaccgt ccggtcgaag cgtcctgtga cggttccgcc tcacgtcgcg gagatgatcc
     6241 gagcgcacat gaaggaccgt acgaagatga acaagggccc cgaggcattc ctggtgacc
//
