LOCUS       sequence-345665-        5561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0789:Dendra
FEATURES             Location/Qualifiers
     source          1..5561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     685..704
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        685..703
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2144..2167
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2424..3224
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3349..3937
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3838..3857
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcgaagtcc gcgttcacca agtcgctgaa gcgtggctac gccaagatcg gtcggccgga
       61 actccgcatc cacgacctcc gcgctgtcgg cgctacgttc gccgctcagg caggtgcgac
      121 gaccaaggag ctgatggccc gtctcggtca cacgactcct aggatggcga tgaagtacca
      181 gatggcgtct gaggcccgcg acgaggctat cgctgaggcg atgtccaagc tggccaagac
      241 ctcctgaaac gcaaaaagcc cccctcccaa ggacactgag tcctaaagag gggggtttct
      301 tgtcagtacg cgaagaacca cgcctggccg cgagcgccag caccgccgct ctgtgcggag
      361 acctgggcac cagccccgcc gccgccagga gcattgccgt tcccgccaga aatctagacg
      421 gtgaccacaa cgcgcccgct ttgatcgggg acgtcgaaat attggatcgt cggcaccgtc
      481 acggccgtgg gaggcggcac gatccgcgac gtgatgatcg gccgcatccc cacggtgctg
      541 cgcagtgagc tctacgccat cccggcgttg atctgtgcgt tcgcacgcac aggcccggtg
      601 tgagaagggt ctctgacgag cgggagaact ccctatcagt gatagagttt gtcctcccta
      661 tcagtgatag ataggcttaa ggagtaatac gactcactat agggagaagg aggccattat
      721 ggaccaagcc gtcgaacttc ccgtccagcg cctgcagcgc gcctcgttgt atctgtgcac
      781 cgacgcccgt cgtgaacgcg gtgacctggc cgaattcgcc gacgcggcac tcgccggcgg
      841 cgtcgacctg atccagctgc gtgacaaggg ctccgcgggt gagaaacagt tcgggccgct
      901 tgaggcccgt caggaactcg aagcgctcga gatcctggcc gacgccgcac gccgccacgg
      961 cgctctgctg gccgtcaacg atcgcgccga tatcgccctg gccgccgggg ccgacgtgct
     1021 gcatctcggc caggacgacc tgccgctgga tgtcgcgcgc ggcatcatcg gccgcaggcc
     1081 ggtcatcgga cgctcgaccc atgacgccgc gcagatggcc gtggccatcg aggagcgggt
     1141 ggactacttc tgcgtcgggc cgtgctggcc gacacccacc aaaccggggc gtcccgcgcc
     1201 gggtctggat ctggtgcggg ccaccgctgc gcacagtccc ggcaagccgt ggttcgcgat
     1261 cggcggcatc gaccaggagc gtctgcccga ggtgctcgcc gcgggcgcgc ggcgagtcgt
     1321 cgtcgtgcgc gcgatcaccg ctgccgacga cccgaaggcc gccgccgagg acctcaaggc
     1381 cgcgatcagc gctgccggta agcttggtgc ggccgcgggc aacacgcctg gcatcaatct
     1441 gatcaaggag gacatgcgtg tcaaggtgca tatggagggc aacgtgaacg gtcacgcctt
     1501 cgtgatcgag ggggagggta agggtaagcc ttacgaaggg acccagaccg ccaacctcac
     1561 cgtcaaggag ggcgcccctc tcccattctc gtacgatatc ctcactaccg cggtgcatta
     1621 cgggaaccgc gtgttcacca agtaccccga ggatatccct gactacttca agcagagctt
     1681 cccggaggga tactcctggg agcggacgat gaccttcgaa gacaagggta tctgtacgat
     1741 ccgcagcgac atctcgctgg aaggggactg tttcttccag aacgtccgtt tcaaggggac
     1801 caactttccg ccgaacggcc cggtcatgca gaagaagacc ctcaagtggg agccgagcac
     1861 ggagaagctg cacgttcggg acggcctcct ggtcggcaac atcaacatgg cgctgctgct
     1921 ggaaggcggc ggccactacc tatgcgactt caagaccacg tacaaagcga agaaggtcgt
     1981 ccagctgccc gacgcgcact tcgtggacca ccgcatcgaa atcctgggga acgattcgga
     2041 ctacaacaag gtcaagttgt acgagcacgc tgtggcacgc tatagcccgc tcccgtcgca
     2101 ggtctggttc gaaggcggcg gcggggagaa cctgtatttc caggactaca aggacgacga
     2161 cgacaagtga tttaaataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt
     2221 aactagcgta cgatcgaatt cctgcagctc acggtaactg atgccgtatt tgcagtacca
     2281 gcgtacggcc cacagaatga tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg
     2341 cagctccatc agcaaaaggg gatgataagt ttatcaccac cgactatttg caacagtgcc
     2401 gttgatcgtg ctatgatcga ctgatgtcat cagcggtgga gtgcaatgtc gtgcaatacg
     2461 aatggcgaaa agccgagctc atcggtcagc ttctcaacct tggggttacc cccggcggtg
     2521 tgctgctggt ccacagctcc ttccgtagcg tccggcccct cgaagatggg ccacttggac
     2581 tgatcgaggc cctgcgtgct acgctgggtc cgggagggac gctcgtcatg ccctcgtggt
     2641 caggtctgga cgacgagccg ttcgatcctg ccacgtcgcc cgttacaccg gaccttggag
     2701 ttgtctctga cacattctgg cgcctgccaa atgtaaagcg cagcgcccat ccatttgcct
     2761 ttgcggcagc ggggccacag gcagagcaga tcatctctga tccattgccc ctgccacctt
     2821 actcgcctgc aagcccggtc gcccgtgtcc atgaactcga tgggcaggta cttctcctcg
     2881 gcgtgggaca cgatgccaac acgacgctgc atcttgccga gttgatggca aaggttccct
     2941 atggggtgcc gagacactgc accattcttc aggatggcaa gttggtacgc gtcgattatc
     3001 tcgagaatga ccactgctgt gagcgctttg ccttggcgga caggtggctc aaggagaaga
     3061 gccttcagaa ggaaggtcca gtcggtcatg cctttgctcg gttgatccgc tcccgcgaca
     3121 ttgtggcgac agccctgggt caactgggcc gagatccgtt gatcttcctg catccgccag
     3181 aggcgggatg cgaagaatgc gatgccgctc gccagtcgat tggctgagct catgagcgga
     3241 gaacgagatg acgttggagg ggcaaggtcg cgctgattgc tggggcaaca cgggggatcc
     3301 actagttcca tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3361 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3421 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3481 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3541 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3601 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3661 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3721 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgagggga gaaaggcgga
     3781 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3841 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3901 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3961 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     4021 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     4081 gaccgagcgc aacgcgtgcg gccgcggtac ccggggatcc tctagagtcg accaccaagg
     4141 gcaccatctc tgcttgggcc accccgttgg ccgcagccag ctcgctgaga gccgtgaacg
     4201 acagggcgaa cgccagcccg ccgacggcga gggttccgac cgctgcaact cccggtgcaa
     4261 ccttgtcccg gtctattctc ttcactgcac cagctccaat ctggtgtgaa tgcccctcgt
     4321 ctgttcgcgc aggcgggggg ctctattcgt ttgtcagcat cgaaagtagc cagatcaggg
     4381 atgcgttgca accgcgtatg cccaggtcag aagagtcgca caagagttgc agacccctgg
     4441 aaagaaaaat ggccagaggg cgaaaacacc ctctgaccag cggagcgggc gacgggaatc
     4501 gaacccgcgt agctagtttg gaagaatggg tgtctgccga ccacatatgg gccggtcaag
     4561 ataggttttt accccctctc ggctgcatcc tctaagtgga aagaaattgc aggtcgtaga
     4621 agcgcgttga agcctgagag ttgcacagga gttgcaaccc ggtagccttg ttcacgacga
     4681 gaggagacct agttggcacg tcgcggatgg ggatcgctga agactcagcg cagcgggagg
     4741 atccaagcct catacgtcaa cccgcaggac ggtgtgaggt actacgcgct gcagacctac
     4801 gacaacaaga tggacgccga agcctggctc gcgggcgaga agcggctcat cgagatggag
     4861 acctggaccc ctccacagga ccgggcgaag aaggcagccg ccagcgccat cacgctggag
     4921 gagtacaccc ggaagtggct cgtggagcgc gacctcgcag acggcaccag ggatctgtac
     4981 agcgggcacg cggagcgccg catctacccg gtgctaggtg aagtggcggt cacagagatg
     5041 acgccagctc tggtgcgtgc gtggtgggcc gggatgggta ggaagcaccc gactgcccgc
     5101 cggcatgcct acaacgtcct ccgggcggtg atgaacacag cggtcgagga caagctgatc
     5161 gcagagaacc cgtgccggat cgagcagaag gcagccgatg agcgcgacgt agaggcgctg
     5221 acgcctgagg agctggacat cgtcgccgct gagatcttcg agcactaccg gatcgcggca
     5281 tacatcctgg cgtggacgag cctccggttc ggagagctga tcgagcttcg ccgcaaggac
     5341 atcgtggacg acggcatgac gatgaagctc cgggtgcgcc gtggcgcttc ccgcgtgggg
     5401 aacaagatcg tcgttggcaa cgccaagacc gtccggtcga agcgtcctgt gacggttccg
     5461 cctcacgtcg cggagatgat ccgagcgcac atgaaggacc gtacgaagat gaacaagggc
     5521 cccgaggcat tcctggtgac cacgacgcag ggcaaccggc t
//
