LOCUS       sequence-345667-        5576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0861:Dendra
FEATURES             Location/Qualifiers
     source          1..5576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     715..734
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        715..733
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2189..2212
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2469..3269
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3394..3982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3883..3902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctggtgacc acgacgcagg gcaaccggct gtcgaagtcc gcgttcacca agtcgctgaa
       61 gcgtggctac gccaagatcg gtcggccgga actccgcatc cacgacctcc gcgctgtcgg
      121 cgctacgttc gccgctcagg caggtgcgac gaccaaggag ctgatggccc gtctcggtca
      181 cacgactcct aggatggcga tgaagtacca gatggcgtct gaggcccgcg acgaggctat
      241 cgctgaggcg atgtccaagc tggccaagac ctcctgaaac gcaaaaagcc cccctcccaa
      301 ggacactgag tcctaaagag gggggtttct tgtcagtacg cgaagaacca cgcctggccg
      361 cgagcgccag caccgccgct ctgtgcggag acctgggcac cagccccgcc gccgccagga
      421 gcattgccgt tcccgccaga aatctagacg gtgaccacaa cgcgcccgct ttgatcgggg
      481 acgtcgaaat attggatcgt cggcaccgtc acggccgtgg gaggcggcac gatccgcgac
      541 gtgatgatcg gccgcatccc cacggtgctg cgcagtgagc tctacgccat cccggcgttg
      601 atctgtgcgt tcgcacgcac aggcccggtg tgagaagggt ctctgacgag cgggagaact
      661 ccctatcagt gatagagttt gtcctcccta tcagtgatag ataggcttaa ggagtaatac
      721 gactcactat agggagaagg aggccattat ggccagacgc cccgatctcc aatccggccc
      781 agaacgcctc gcggcgctgg tgcgctccag cattcccccg atgcattcgg cgggcctgcc
      841 tttcgtcggc gcgagcctcg cggtcgccct gctcggccgc aagcggcgct ggatgcggcg
      901 cgcgggactg atctcggcgg gcgccaacgc cgcgttcttc cggcacccgc cgcgggtccc
      961 accgacccgg ccgggcgtcg tggtggcgcc ggccgacggg ctgatctgcc tgctgggcga
     1021 ggccacgccc ccggccgaac tgggattgcc ggatatcccg atgcaacgcg tcagcatctt
     1081 cctgtcggtg ctcgacgcgc acgtgcagcg cgcgccgatc ggcggcgagg tggtcgccgt
     1141 gcgtcaccgg ccgggccgct tccactcagc cgaactggaa gccgccagtg aggacaacga
     1201 acgcaacagc gtcgtcatcc gcacccccga gggcctgcac atcatcgcgg tgcagatcgc
     1261 cggtctgatc gcgcggcgca tcgtgtgcga cgtccacgtc ggcgacaagc tctcgatcgg
     1321 cgacacctac ggcctgatcc gctacggttc gcgcctggac acctacttcc ctgcggacgc
     1381 cagggtgctc gtctctcacg gtcagcgcac cctggccggc gaaacggtgc tggcggagct
     1441 cgcaaagctt ggtgcggccg cgggcaacac gcctggcatc aatctgatca aggaggacat
     1501 gcgtgtcaag gtgcatatgg agggcaacgt gaacggtcac gccttcgtga tcgaggggga
     1561 gggtaagggt aagccttacg aagggaccca gaccgccaac ctcaccgtca aggagggcgc
     1621 ccctctccca ttctcgtacg atatcctcac taccgcggtg cattacggga accgcgtgtt
     1681 caccaagtac cccgaggata tccctgacta cttcaagcag agcttcccgg agggatactc
     1741 ctgggagcgg acgatgacct tcgaagacaa gggtatctgt acgatccgca gcgacatctc
     1801 gctggaaggg gactgtttct tccagaacgt ccgtttcaag gggaccaact ttccgccgaa
     1861 cggcccggtc atgcagaaga agaccctcaa gtgggagccg agcacggaga agctgcacgt
     1921 tcgggacggc ctcctggtcg gcaacatcaa catggcgctg ctgctggaag gcggcggcca
     1981 ctacctatgc gacttcaaga ccacgtacaa agcgaagaag gtcgtccagc tgcccgacgc
     2041 gcacttcgtg gaccaccgca tcgaaatcct ggggaacgat tcggactaca acaaggtcaa
     2101 gttgtacgag cacgctgtgg cacgctatag cccgctcccg tcgcaggtct ggttcgaagg
     2161 cggcggcggg gagaacctgt atttccagga ctacaaggac gacgacgaca agtgatttaa
     2221 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgttaacta gcgtacgatc
     2281 gaattcctgc agctcacggt aactgatgcc gtatttgcag taccagcgta cggcccacag
     2341 aatgatgtca cgctgaaaat gccggccttt gaatgggttc atgtgcagct ccatcagcaa
     2401 aaggggatga taagtttatc accaccgact atttgcaaca gtgccgttga tcgtgctatg
     2461 atcgactgat gtcatcagcg gtggagtgca atgtcgtgca atacgaatgg cgaaaagccg
     2521 agctcatcgg tcagcttctc aaccttgggg ttacccccgg cggtgtgctg ctggtccaca
     2581 gctccttccg tagcgtccgg cccctcgaag atgggccact tggactgatc gaggccctgc
     2641 gtgctacgct gggtccggga gggacgctcg tcatgccctc gtggtcaggt ctggacgacg
     2701 agccgttcga tcctgccacg tcgcccgtta caccggacct tggagttgtc tctgacacat
     2761 tctggcgcct gccaaatgta aagcgcagcg cccatccatt tgcctttgcg gcagcggggc
     2821 cacaggcaga gcagatcatc tctgatccat tgcccctgcc accttactcg cctgcaagcc
     2881 cggtcgcccg tgtccatgaa ctcgatgggc aggtacttct cctcggcgtg ggacacgatg
     2941 ccaacacgac gctgcatctt gccgagttga tggcaaaggt tccctatggg gtgccgagac
     3001 actgcaccat tcttcaggat ggcaagttgg tacgcgtcga ttatctcgag aatgaccact
     3061 gctgtgagcg ctttgccttg gcggacaggt ggctcaagga gaagagcctt cagaaggaag
     3121 gtccagtcgg tcatgccttt gctcggttga tccgctcccg cgacattgtg gcgacagccc
     3181 tgggtcaact gggccgagat ccgttgatct tcctgcatcc gccagaggcg ggatgcgaag
     3241 aatgcgatgc cgctcgccag tcgattggct gagctcatga gcggagaacg agatgacgtt
     3301 ggaggggcaa ggtcgcgctg attgctgggg caacacgggg gatccactag ttccatgagc
     3361 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3421 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3481 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3541 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3601 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3661 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3721 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3781 tgagctatga gaaagcgcca cgcttcccga ggggagaaag gcggacaggt atccggtaag
     3841 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3901 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3961 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4021 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4081 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc
     4141 gtgcggccgc ggtacccggg gatcctctag agtcgaccac caagggcacc atctctgctt
     4201 gggccacccc gttggccgca gccagctcgc tgagagccgt gaacgacagg gcgaacgcca
     4261 gcccgccgac ggcgagggtt ccgaccgctg caactcccgg tgcaaccttg tcccggtcta
     4321 ttctcttcac tgcaccagct ccaatctggt gtgaatgccc ctcgtctgtt cgcgcaggcg
     4381 gggggctcta ttcgtttgtc agcatcgaaa gtagccagat cagggatgcg ttgcaaccgc
     4441 gtatgcccag gtcagaagag tcgcacaaga gttgcagacc cctggaaaga aaaatggcca
     4501 gagggcgaaa acaccctctg accagcggag cgggcgacgg gaatcgaacc cgcgtagcta
     4561 gtttggaaga atgggtgtct gccgaccaca tatgggccgg tcaagatagg tttttacccc
     4621 ctctcggctg catcctctaa gtggaaagaa attgcaggtc gtagaagcgc gttgaagcct
     4681 gagagttgca caggagttgc aacccggtag ccttgttcac gacgagagga gacctagttg
     4741 gcacgtcgcg gatggggatc gctgaagact cagcgcagcg ggaggatcca agcctcatac
     4801 gtcaacccgc aggacggtgt gaggtactac gcgctgcaga cctacgacaa caagatggac
     4861 gccgaagcct ggctcgcggg cgagaagcgg ctcatcgaga tggagacctg gacccctcca
     4921 caggaccggg cgaagaaggc agccgccagc gccatcacgc tggaggagta cacccggaag
     4981 tggctcgtgg agcgcgacct cgcagacggc accagggatc tgtacagcgg gcacgcggag
     5041 cgccgcatct acccggtgct aggtgaagtg gcggtcacag agatgacgcc agctctggtg
     5101 cgtgcgtggt gggccgggat gggtaggaag cacccgactg cccgccggca tgcctacaac
     5161 gtcctccggg cggtgatgaa cacagcggtc gaggacaagc tgatcgcaga gaacccgtgc
     5221 cggatcgagc agaaggcagc cgatgagcgc gacgtagagg cgctgacgcc tgaggagctg
     5281 gacatcgtcg ccgctgagat cttcgagcac taccggatcg cggcatacat cctggcgtgg
     5341 acgagcctcc ggttcggaga gctgatcgag cttcgccgca aggacatcgt ggacgacggc
     5401 atgacgatga agctccgggt gcgccgtggc gcttcccgcg tggggaacaa gatcgtcgtt
     5461 ggcaacgcca agaccgtccg gtcgaagcgt cctgtgacgg ttccgcctca cgtcgcggag
     5521 atgatccgag cgcacatgaa ggaccgtacg aagatgaaca agggccccga ggcatt
//
