LOCUS       sequence-345668-        5726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0909:Dendra
FEATURES             Location/Qualifiers
     source          1..5726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     748..767
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        748..766
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2372..2395
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2652..3452
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3577..4165
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4066..4085
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgtacgaag atgaacaagg gccccgaggc attcctggtg accacgacgc agggcaaccg
       61 gctgtcgaag tccgcgttca ccaagtcgct gaagcgtggc tacgccaaga tcggtcggcc
      121 ggaactccgc atccacgacc tccgcgctgt cggcgctacg ttcgccgctc aggcaggtgc
      181 gacgaccaag gagctgatgg cccgtctcgg tcacacgact cctaggatgg cgatgaagta
      241 ccagatggcg tctgaggccc gcgacgaggc tatcgctgag gcgatgtcca agctggccaa
      301 gacctcctga aacgcaaaaa gcccccctcc caaggacact gagtcctaaa gaggggggtt
      361 tcttgtcagt acgcgaagaa ccacgcctgg ccgcgagcgc cagcaccgcc gctctgtgcg
      421 gagacctggg caccagcccc gccgccgcca ggagcattgc cgttcccgcc agaaatctag
      481 acggtgacca caacgcgccc gctttgatcg gggacgtcga aatattggat cgtcggcacc
      541 gtcacggccg tgggaggcgg cacgatccgc gacgtgatga tcggccgcat ccccacggtg
      601 ctgcgcagtg agctctacgc catcccggcg ttgatctgtg cgttcgcacg cacaggcccg
      661 gtgtgagaag ggtctctgac gagcgggaga actccctatc agtgatagag tttgtcctcc
      721 ctatcagtga tagataggct taaggagtaa tacgactcac tatagggaga aggaggccat
      781 tatgacgggc accgagaagg gcagcaaaga cagcagtgag aacagtctgg tgagcacggg
      841 tctggccaag acgatgatgc ccgtaccgga cccgcacccc gacgtgttcg acaccggctg
      901 gccattgcgc gtcgccgaca tcgaccgcaa cgggcggctg cgcttcgatg cctcgacccg
      961 gcacatccag gacatcggcc aggatcatct gcgccagttg gggttcgagg acacccatcc
     1021 ggcctggatc gtgcgccgca ccatgatcga catgatcgag cccatcgagt tccccgaact
     1081 gctgcggctg cgccgctggt gctcgggcac ctcgaaccgg tggtgcgaga tgcgcgtgcg
     1141 catcgacgga cgcaagggcg gcctggtcga gtccgaggcg ttctggatca acatcaaccg
     1201 cgagacccag ggcccgtcgc ggatcgccga cgacttcctg gccggactgc ggcgcaccac
     1261 cgacatcgac cggctgcgct ggaagcccta tctcaaggcg ggcagccgcg aggacgcgct
     1321 ggagatccgc gagtacccgg tgcgcgtcgc cgacatcgac ctgttcgacc acatgaacaa
     1381 cgccgtctac tggaccgtgg tcgaggacta cctgtacacg catcccgagc tgctcgcgca
     1441 gccgttgcgc gtgaccatcg agcacgacgc tcccgtggcg ctcggcgaca agctggagat
     1501 catctcccac acccacccgc cgggtacgac cgacaaattc ggtccggaac tcaccgatcg
     1561 gactgttaca accctcacat atgcggtcgg cgaggagacg aaagccgtcg cctgtctctt
     1621 ctcgctcaag cttggtgcgg ccgcgggcaa cacgcctggc atcaatctga tcaaggagga
     1681 catgcgtgtc aaggtgcata tggagggcaa cgtgaacggt cacgccttcg tgatcgaggg
     1741 ggagggtaag ggtaagcctt acgaagggac ccagaccgcc aacctcaccg tcaaggaggg
     1801 cgcccctctc ccattctcgt acgatatcct cactaccgcg gtgcattacg ggaaccgcgt
     1861 gttcaccaag taccccgagg atatccctga ctacttcaag cagagcttcc cggagggata
     1921 ctcctgggag cggacgatga ccttcgaaga caagggtatc tgtacgatcc gcagcgacat
     1981 ctcgctggaa ggggactgtt tcttccagaa cgtccgtttc aaggggacca actttccgcc
     2041 gaacggcccg gtcatgcaga agaagaccct caagtgggag ccgagcacgg agaagctgca
     2101 cgttcgggac ggcctcctgg tcggcaacat caacatggcg ctgctgctgg aaggcggcgg
     2161 ccactaccta tgcgacttca agaccacgta caaagcgaag aaggtcgtcc agctgcccga
     2221 cgcgcacttc gtggaccacc gcatcgaaat cctggggaac gattcggact acaacaaggt
     2281 caagttgtac gagcacgctg tggcacgcta tagcccgctc ccgtcgcagg tctggttcga
     2341 aggcggcggc ggggagaacc tgtatttcca ggactacaag gacgacgacg acaagtgatt
     2401 taaataaccc cttggggcct ctaaacgggt cttgaggggt tttttgttaa ctagcgtacg
     2461 atcgaattcc tgcagctcac ggtaactgat gccgtatttg cagtaccagc gtacggccca
     2521 cagaatgatg tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca gctccatcag
     2581 caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt tgatcgtgct
     2641 atgatcgact gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag
     2701 ccgagctcat cggtcagctt ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc
     2761 acagctcctt ccgtagcgtc cggcccctcg aagatgggcc acttggactg atcgaggccc
     2821 tgcgtgctac gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg
     2881 acgagccgtt cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt gtctctgaca
     2941 cattctggcg cctgccaaat gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg
     3001 ggccacaggc agagcagatc atctctgatc cattgcccct gccaccttac tcgcctgcaa
     3061 gcccggtcgc ccgtgtccat gaactcgatg ggcaggtact tctcctcggc gtgggacacg
     3121 atgccaacac gacgctgcat cttgccgagt tgatggcaaa ggttccctat ggggtgccga
     3181 gacactgcac cattcttcag gatggcaagt tggtacgcgt cgattatctc gagaatgacc
     3241 actgctgtga gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc cttcagaagg
     3301 aaggtccagt cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt gtggcgacag
     3361 ccctgggtca actgggccga gatccgttga tcttcctgca tccgccagag gcgggatgcg
     3421 aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga acgagatgac
     3481 gttggagggg caaggtcgcg ctgattgctg gggcaacacg ggggatccac tagttccatg
     3541 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3601 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3661 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3721 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3781 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3841 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3901 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3961 gcgtgagcta tgagaaagcg ccacgcttcc cgaggggaga aaggcggaca ggtatccggt
     4021 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4081 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4141 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4201 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4261 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcaa
     4321 cgcgtgcggc cgcggtaccc ggggatcctc tagagtcgac caccaagggc accatctctg
     4381 cttgggccac cccgttggcc gcagccagct cgctgagagc cgtgaacgac agggcgaacg
     4441 ccagcccgcc gacggcgagg gttccgaccg ctgcaactcc cggtgcaacc ttgtcccggt
     4501 ctattctctt cactgcacca gctccaatct ggtgtgaatg cccctcgtct gttcgcgcag
     4561 gcggggggct ctattcgttt gtcagcatcg aaagtagcca gatcagggat gcgttgcaac
     4621 cgcgtatgcc caggtcagaa gagtcgcaca agagttgcag acccctggaa agaaaaatgg
     4681 ccagagggcg aaaacaccct ctgaccagcg gagcgggcga cgggaatcga acccgcgtag
     4741 ctagtttgga agaatgggtg tctgccgacc acatatgggc cggtcaagat aggtttttac
     4801 cccctctcgg ctgcatcctc taagtggaaa gaaattgcag gtcgtagaag cgcgttgaag
     4861 cctgagagtt gcacaggagt tgcaacccgg tagccttgtt cacgacgaga ggagacctag
     4921 ttggcacgtc gcggatgggg atcgctgaag actcagcgca gcgggaggat ccaagcctca
     4981 tacgtcaacc cgcaggacgg tgtgaggtac tacgcgctgc agacctacga caacaagatg
     5041 gacgccgaag cctggctcgc gggcgagaag cggctcatcg agatggagac ctggacccct
     5101 ccacaggacc gggcgaagaa ggcagccgcc agcgccatca cgctggagga gtacacccgg
     5161 aagtggctcg tggagcgcga cctcgcagac ggcaccaggg atctgtacag cgggcacgcg
     5221 gagcgccgca tctacccggt gctaggtgaa gtggcggtca cagagatgac gccagctctg
     5281 gtgcgtgcgt ggtgggccgg gatgggtagg aagcacccga ctgcccgccg gcatgcctac
     5341 aacgtcctcc gggcggtgat gaacacagcg gtcgaggaca agctgatcgc agagaacccg
     5401 tgccggatcg agcagaaggc agccgatgag cgcgacgtag aggcgctgac gcctgaggag
     5461 ctggacatcg tcgccgctga gatcttcgag cactaccgga tcgcggcata catcctggcg
     5521 tggacgagcc tccggttcgg agagctgatc gagcttcgcc gcaaggacat cgtggacgac
     5581 ggcatgacga tgaagctccg ggtgcgccgt ggcgcttccc gcgtggggaa caagatcgtc
     5641 gttggcaacg ccaagaccgt ccggtcgaag cgtcctgtga cggttccgcc tcacgtcgcg
     5701 gagatgatcc gagcgcacat gaagga
//
