LOCUS       sequence-34567-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are 
            expressed as a single poly-cistronic unit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Chou BK, Mali P, Huang X, Ye Z, Dowey SN, Resar LM, Zou C, Zhang YA,
            Tong J, Cheng L
  TITLE     Efficient human iPS cell derivation by a non-integrating plasmid 
            from blood cells with unique epigenetic and gene expression 
            signatures.
  JOURNAL   Cell Res. 2011 Mar;21(3):518-29. doi: 10.1038/cr.2011.12. Epub 2011 
            Jan 18.
  PUBMED    21243013
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cr.2011.12"; journalName: "Cell Res"; date: "2011-03"; 
            volume: "21"; issue: "3"; pages: "518-29"
COMMENT     Sequence Label: pEB-C5
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..78
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tcaaacggcg gcgggtccgg aggagagggc agaggaagtc ttctaacatg cggtgacgtg
       61 gaggagaatc ctggcccaat gtataacatg atggagacgg agctgaagcc gccgggcccg
      121 cagcaagctt cggggggcgg cggcggagga ggcaacgcca cggcggcggc gaccggcggc
      181 aaccagaaga acagcccgga ccgcgtcaag aggcccatga acgccttcat ggtatggtcc
      241 cgggggcagc ggcgtaagat ggcccaggag aaccccaaga tgcacaactc ggagatcagc
      301 aagcgcctgg gcgcggagtg gaaacttttg tccgagaccg agaagcggcc gttcatcgac
      361 gaggccaagc ggctgcgcgc tctgcacatg aaggagcacc cggattataa ataccggccg
      421 cggcggaaaa ccaagacgct catgaagaag gataagtaca cgcttcccgg aggcttgctg
      481 gcccccggcg ggaacagcat ggcgagcggg gttggggtgg gcgccggcct gggtgcgggc
      541 gtgaaccagc gcatggacag ctacgcgcac atgaacggct ggagcaacgg cagctacagc
      601 atgatgcagg agcagctggg ctacccgcag cacccgggcc tcaacgctca cggcgcggca
      661 cagatgcaac cgatgcaccg ctacgacgtc agcgccctgc agtacaactc catgaccagc
      721 tcgcagacct acatgaacgg ctcgcccacc tacagcatgt cctactcgca gcagggcacc
      781 cccggtatgg cgctgggctc catgggctct gtggtca
//
