LOCUS       sequence-345675-        5906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0946:Dendra
FEATURES             Location/Qualifiers
     source          1..5906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     757..776
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        757..775
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2561..2584
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2841..3641
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3766..4354
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4255..4274
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgaaggac cgtacgaaga tgaacaaggg ccccgaggca ttcctggtga ccacgacgca
       61 gggcaaccgg ctgtcgaagt ccgcgttcac caagtcgctg aagcgtggct acgccaagat
      121 cggtcggccg gaactccgca tccacgacct ccgcgctgtc ggcgctacgt tcgccgctca
      181 ggcaggtgcg acgaccaagg agctgatggc ccgtctcggt cacacgactc ctaggatggc
      241 gatgaagtac cagatggcgt ctgaggcccg cgacgaggct atcgctgagg cgatgtccaa
      301 gctggccaag acctcctgaa acgcaaaaag cccccctccc aaggacactg agtcctaaag
      361 aggggggttt cttgtcagta cgcgaagaac cacgcctggc cgcgagcgcc agcaccgccg
      421 ctctgtgcgg agacctgggc accagccccg ccgccgccag gagcattgcc gttcccgcca
      481 gaaatctaga cggtgaccac aacgcgcccg ctttgatcgg ggacgtcgaa atattggatc
      541 gtcggcaccg tcacggccgt gggaggcggc acgatccgcg acgtgatgat cggccgcatc
      601 cccacggtgc tgcgcagtga gctctacgcc atcccggcgt tgatctgtgc gttcgcacgc
      661 acaggcccgg tgtgagaagg gtctctgacg agcgggagaa ctccctatca gtgatagagt
      721 ttgtcctccc tatcagtgat agataggctt aaggagtaat acgactcact atagggagaa
      781 ggaggccatt atggttctgg tcacgggtgc gtgccggttc ctcggcggtt acctgaccgc
      841 gaggctggcg cagaacccgt cgatcgacca tgtcatcgcg gtcgacgcga tcacgcccag
      901 caaggatttg ctgcgaagga tggggcgcgc ggagttcgtg cgcgccgaca tccgcaatcc
      961 gttcatcgcc aaggtgatcc gcaacggcaa cgtcgacacc gtcgtgcacg ccgcggccgc
     1021 gtcgtacgcg ccccgctcgg gtggccgggc cacgttgaaa gagctcaacg tgatgggggc
     1081 catccagttg ttcgcggcgt gccagaagac gccgtcggta cgccgggtca tcctcaagtc
     1141 gacctccgag gtctacgggt cgagttcgcg cgatccggtg atgttcaccg aggagagcag
     1201 cgcgcgccgt ccgccgcgcg atggcttcgc ccgcgacagc atcgacatcg agggctacgc
     1261 gcgaggtctc gcccggcgtc gtcccgacat cgccgtcacg atcctgcggc tggccaacat
     1321 gatcggcccc gcgatggaca cggcgctgtc gcgctatctg gccgggccgg tggtgccctg
     1381 cgtcgtcggg cacgacgcgc ggctgcaact gctgcacgag caggacgcgc tcggtgccct
     1441 cgagcacgcc accgtggcag gcaaggccgg cacgttcaac atcggcgccg acggcataat
     1501 catgatgagt caggcgctgc gccgctcggg ccggatccgg ctcccggtgc cgagatcggc
     1561 actcgccgca gtggattcgc tgaacagggc cacgcgttac agcgaggtgg atcgtgaaca
     1621 actcaactat ctgagttacg gccgcgtcat ggataccacg cgaatgcgcc gcgacctggg
     1681 atacagtccc aagtggacga cgggggaagc gttcgacgat tacgttcgtg gccgtggatt
     1741 gacgccgatc atcgatccgc ggtgggtagg ctcgctggag agtcgtgcag ttgccttggc
     1801 gcagcgttgg ggacggaagc ttggtgcggc cgcgggcaac acgcctggca tcaatctgat
     1861 caaggaggac atgcgtgtca aggtgcatat ggagggcaac gtgaacggtc acgccttcgt
     1921 gatcgagggg gagggtaagg gtaagcctta cgaagggacc cagaccgcca acctcaccgt
     1981 caaggagggc gcccctctcc cattctcgta cgatatcctc actaccgcgg tgcattacgg
     2041 gaaccgcgtg ttcaccaagt accccgagga tatccctgac tacttcaagc agagcttccc
     2101 ggagggatac tcctgggagc ggacgatgac cttcgaagac aagggtatct gtacgatccg
     2161 cagcgacatc tcgctggaag gggactgttt cttccagaac gtccgtttca aggggaccaa
     2221 ctttccgccg aacggcccgg tcatgcagaa gaagaccctc aagtgggagc cgagcacgga
     2281 gaagctgcac gttcgggacg gcctcctggt cggcaacatc aacatggcgc tgctgctgga
     2341 aggcggcggc cactacctat gcgacttcaa gaccacgtac aaagcgaaga aggtcgtcca
     2401 gctgcccgac gcgcacttcg tggaccaccg catcgaaatc ctggggaacg attcggacta
     2461 caacaaggtc aagttgtacg agcacgctgt ggcacgctat agcccgctcc cgtcgcaggt
     2521 ctggttcgaa ggcggcggcg gggagaacct gtatttccag gactacaagg acgacgacga
     2581 caagtgattt aaataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgttaac
     2641 tagcgtacga tcgaattcct gcagctcacg gtaactgatg ccgtatttgc agtaccagcg
     2701 tacggcccac agaatgatgt cacgctgaaa atgccggcct ttgaatgggt tcatgtgcag
     2761 ctccatcagc aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgccgtt
     2821 gatcgtgcta tgatcgactg atgtcatcag cggtggagtg caatgtcgtg caatacgaat
     2881 ggcgaaaagc cgagctcatc ggtcagcttc tcaaccttgg ggttaccccc ggcggtgtgc
     2941 tgctggtcca cagctccttc cgtagcgtcc ggcccctcga agatgggcca cttggactga
     3001 tcgaggccct gcgtgctacg ctgggtccgg gagggacgct cgtcatgccc tcgtggtcag
     3061 gtctggacga cgagccgttc gatcctgcca cgtcgcccgt tacaccggac cttggagttg
     3121 tctctgacac attctggcgc ctgccaaatg taaagcgcag cgcccatcca tttgcctttg
     3181 cggcagcggg gccacaggca gagcagatca tctctgatcc attgcccctg ccaccttact
     3241 cgcctgcaag cccggtcgcc cgtgtccatg aactcgatgg gcaggtactt ctcctcggcg
     3301 tgggacacga tgccaacacg acgctgcatc ttgccgagtt gatggcaaag gttccctatg
     3361 gggtgccgag acactgcacc attcttcagg atggcaagtt ggtacgcgtc gattatctcg
     3421 agaatgacca ctgctgtgag cgctttgcct tggcggacag gtggctcaag gagaagagcc
     3481 ttcagaagga aggtccagtc ggtcatgcct ttgctcggtt gatccgctcc cgcgacattg
     3541 tggcgacagc cctgggtcaa ctgggccgag atccgttgat cttcctgcat ccgccagagg
     3601 cgggatgcga agaatgcgat gccgctcgcc agtcgattgg ctgagctcat gagcggagaa
     3661 cgagatgacg ttggaggggc aaggtcgcgc tgattgctgg ggcaacacgg gggatccact
     3721 agttccatga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3781 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3841 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3901 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3961 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4021 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4081 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4141 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaggggagaa aggcggacag
     4201 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4261 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4321 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4381 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4441 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4501 cgagcgcaac gcgtgcggcc gcggtacccg gggatcctct agagtcgacc accaagggca
     4561 ccatctctgc ttgggccacc ccgttggccg cagccagctc gctgagagcc gtgaacgaca
     4621 gggcgaacgc cagcccgccg acggcgaggg ttccgaccgc tgcaactccc ggtgcaacct
     4681 tgtcccggtc tattctcttc actgcaccag ctccaatctg gtgtgaatgc ccctcgtctg
     4741 ttcgcgcagg cggggggctc tattcgtttg tcagcatcga aagtagccag atcagggatg
     4801 cgttgcaacc gcgtatgccc aggtcagaag agtcgcacaa gagttgcaga cccctggaaa
     4861 gaaaaatggc cagagggcga aaacaccctc tgaccagcgg agcgggcgac gggaatcgaa
     4921 cccgcgtagc tagtttggaa gaatgggtgt ctgccgacca catatgggcc ggtcaagata
     4981 ggtttttacc ccctctcggc tgcatcctct aagtggaaag aaattgcagg tcgtagaagc
     5041 gcgttgaagc ctgagagttg cacaggagtt gcaacccggt agccttgttc acgacgagag
     5101 gagacctagt tggcacgtcg cggatgggga tcgctgaaga ctcagcgcag cgggaggatc
     5161 caagcctcat acgtcaaccc gcaggacggt gtgaggtact acgcgctgca gacctacgac
     5221 aacaagatgg acgccgaagc ctggctcgcg ggcgagaagc ggctcatcga gatggagacc
     5281 tggacccctc cacaggaccg ggcgaagaag gcagccgcca gcgccatcac gctggaggag
     5341 tacacccgga agtggctcgt ggagcgcgac ctcgcagacg gcaccaggga tctgtacagc
     5401 gggcacgcgg agcgccgcat ctacccggtg ctaggtgaag tggcggtcac agagatgacg
     5461 ccagctctgg tgcgtgcgtg gtgggccggg atgggtagga agcacccgac tgcccgccgg
     5521 catgcctaca acgtcctccg ggcggtgatg aacacagcgg tcgaggacaa gctgatcgca
     5581 gagaacccgt gccggatcga gcagaaggca gccgatgagc gcgacgtaga ggcgctgacg
     5641 cctgaggagc tggacatcgt cgccgctgag atcttcgagc actaccggat cgcggcatac
     5701 atcctggcgt ggacgagcct ccggttcgga gagctgatcg agcttcgccg caaggacatc
     5761 gtggacgacg gcatgacgat gaagctccgg gtgcgccgtg gcgcttcccg cgtggggaac
     5821 aagatcgtcg ttggcaacgc caagaccgtc cggtcgaagc gtcctgtgac ggttccgcct
     5881 cacgtcgcgg agatgatccg agcgca
//
