LOCUS       sequence-345676-        5375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0934:Dendra
FEATURES             Location/Qualifiers
     source          1..5375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     741..760
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        741..759
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2014..2037
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2294..3094
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3219..3807
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3708..3727
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagatgaaca agggccccga ggcattcctg gtgaccacga cgcagggcaa ccggctgtcg
       61 aagtccgcgt tcaccaagtc gctgaagcgt ggctacgcca agatcggtcg gccggaactc
      121 cgcatccacg acctccgcgc tgtcggcgct acgttcgccg ctcaggcagg tgcgacgacc
      181 aaggagctga tggcccgtct cggtcacacg actcctagga tggcgatgaa gtaccagatg
      241 gcgtctgagg cccgcgacga ggctatcgct gaggcgatgt ccaagctggc caagacctcc
      301 tgaaacgcaa aaagcccccc tcccaaggac actgagtcct aaagaggggg gtttcttgtc
      361 agtacgcgaa gaaccacgcc tggccgcgag cgccagcacc gccgctctgt gcggagacct
      421 gggcaccagc cccgccgccg ccaggagcat tgccgttccc gccagaaatc tagacggtga
      481 ccacaacgcg cccgctttga tcggggacgt cgaaatattg gatcgtcggc accgtcacgg
      541 ccgtgggagg cggcacgatc cgcgacgtga tgatcggccg catccccacg gtgctgcgca
      601 gtgagctcta cgccatcccg gcgttgatct gtgcgttcgc acgcacaggc ccggtgtgag
      661 aagggtctct gacgagcggg agaactccct atcagtgata gagtttgtcc tccctatcag
      721 tgatagatag gcttaaggag taatacgact cactataggg agaaggaggc cattatgaat
      781 gtcaggaaga tcatcgagga caccctcgtc gagcatgagc tcaactacca cttccacgag
      841 ggcgcgaaag gcagcttgcc gggcatcgtc gtcgagctgc ccggtgagcg caagctcaag
      901 accaacacgc tgttgagcat cggcgagcac tccgttcggg tcgaggcgtt cgtgtgccgc
      961 aaacccgacg agaaccacga gggcgtctac cggttcctgc tcaaacgcaa ccgcaggctc
     1021 tacggtgtcg cctacacgct cgacaacgtc ggcgacatct acctcgtggg gcgcatggcg
     1081 ctgcactcgg tgacgcccga cgagatcgac cgcgtgctcg gccaggtact cgaagccgtc
     1141 gattctgact tcaacacgtt gctggagttg ggttttcgct cgtccatcca gaaggaatgg
     1201 gagtggcggg tctcgcgcgg cgagtcgctc aagaacctca aggccttcga gcacctcatc
     1261 gacgagaaga agcttggtgc ggccgcgggc aacacgcctg gcatcaatct gatcaaggag
     1321 gacatgcgtg tcaaggtgca tatggagggc aacgtgaacg gtcacgcctt cgtgatcgag
     1381 ggggagggta agggtaagcc ttacgaaggg acccagaccg ccaacctcac cgtcaaggag
     1441 ggcgcccctc tcccattctc gtacgatatc ctcactaccg cggtgcatta cgggaaccgc
     1501 gtgttcacca agtaccccga ggatatccct gactacttca agcagagctt cccggaggga
     1561 tactcctggg agcggacgat gaccttcgaa gacaagggta tctgtacgat ccgcagcgac
     1621 atctcgctgg aaggggactg tttcttccag aacgtccgtt tcaaggggac caactttccg
     1681 ccgaacggcc cggtcatgca gaagaagacc ctcaagtggg agccgagcac ggagaagctg
     1741 cacgttcggg acggcctcct ggtcggcaac atcaacatgg cgctgctgct ggaaggcggc
     1801 ggccactacc tatgcgactt caagaccacg tacaaagcga agaaggtcgt ccagctgccc
     1861 gacgcgcact tcgtggacca ccgcatcgaa atcctgggga acgattcgga ctacaacaag
     1921 gtcaagttgt acgagcacgc tgtggcacgc tatagcccgc tcccgtcgca ggtctggttc
     1981 gaaggcggcg gcggggagaa cctgtatttc caggactaca aggacgacga cgacaagtga
     2041 tttaaataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt aactagcgta
     2101 cgatcgaatt cctgcagctc acggtaactg atgccgtatt tgcagtacca gcgtacggcc
     2161 cacagaatga tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg cagctccatc
     2221 agcaaaaggg gatgataagt ttatcaccac cgactatttg caacagtgcc gttgatcgtg
     2281 ctatgatcga ctgatgtcat cagcggtgga gtgcaatgtc gtgcaatacg aatggcgaaa
     2341 agccgagctc atcggtcagc ttctcaacct tggggttacc cccggcggtg tgctgctggt
     2401 ccacagctcc ttccgtagcg tccggcccct cgaagatggg ccacttggac tgatcgaggc
     2461 cctgcgtgct acgctgggtc cgggagggac gctcgtcatg ccctcgtggt caggtctgga
     2521 cgacgagccg ttcgatcctg ccacgtcgcc cgttacaccg gaccttggag ttgtctctga
     2581 cacattctgg cgcctgccaa atgtaaagcg cagcgcccat ccatttgcct ttgcggcagc
     2641 ggggccacag gcagagcaga tcatctctga tccattgccc ctgccacctt actcgcctgc
     2701 aagcccggtc gcccgtgtcc atgaactcga tgggcaggta cttctcctcg gcgtgggaca
     2761 cgatgccaac acgacgctgc atcttgccga gttgatggca aaggttccct atggggtgcc
     2821 gagacactgc accattcttc aggatggcaa gttggtacgc gtcgattatc tcgagaatga
     2881 ccactgctgt gagcgctttg ccttggcgga caggtggctc aaggagaaga gccttcagaa
     2941 ggaaggtcca gtcggtcatg cctttgctcg gttgatccgc tcccgcgaca ttgtggcgac
     3001 agccctgggt caactgggcc gagatccgtt gatcttcctg catccgccag aggcgggatg
     3061 cgaagaatgc gatgccgctc gccagtcgat tggctgagct catgagcgga gaacgagatg
     3121 acgttggagg ggcaaggtcg cgctgattgc tggggcaaca cgggggatcc actagttcca
     3181 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3241 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3301 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3361 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3421 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3481 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3541 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3601 cagcgtgagc tatgagaaag cgccacgctt cccgagggga gaaaggcgga caggtatccg
     3661 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3721 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     3781 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     3841 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     3901 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     3961 aacgcgtgcg gccgcggtac ccggggatcc tctagagtcg accaccaagg gcaccatctc
     4021 tgcttgggcc accccgttgg ccgcagccag ctcgctgaga gccgtgaacg acagggcgaa
     4081 cgccagcccg ccgacggcga gggttccgac cgctgcaact cccggtgcaa ccttgtcccg
     4141 gtctattctc ttcactgcac cagctccaat ctggtgtgaa tgcccctcgt ctgttcgcgc
     4201 aggcgggggg ctctattcgt ttgtcagcat cgaaagtagc cagatcaggg atgcgttgca
     4261 accgcgtatg cccaggtcag aagagtcgca caagagttgc agacccctgg aaagaaaaat
     4321 ggccagaggg cgaaaacacc ctctgaccag cggagcgggc gacgggaatc gaacccgcgt
     4381 agctagtttg gaagaatggg tgtctgccga ccacatatgg gccggtcaag ataggttttt
     4441 accccctctc ggctgcatcc tctaagtgga aagaaattgc aggtcgtaga agcgcgttga
     4501 agcctgagag ttgcacagga gttgcaaccc ggtagccttg ttcacgacga gaggagacct
     4561 agttggcacg tcgcggatgg ggatcgctga agactcagcg cagcgggagg atccaagcct
     4621 catacgtcaa cccgcaggac ggtgtgaggt actacgcgct gcagacctac gacaacaaga
     4681 tggacgccga agcctggctc gcgggcgaga agcggctcat cgagatggag acctggaccc
     4741 ctccacagga ccgggcgaag aaggcagccg ccagcgccat cacgctggag gagtacaccc
     4801 ggaagtggct cgtggagcgc gacctcgcag acggcaccag ggatctgtac agcgggcacg
     4861 cggagcgccg catctacccg gtgctaggtg aagtggcggt cacagagatg acgccagctc
     4921 tggtgcgtgc gtggtgggcc gggatgggta ggaagcaccc gactgcccgc cggcatgcct
     4981 acaacgtcct ccgggcggtg atgaacacag cggtcgagga caagctgatc gcagagaacc
     5041 cgtgccggat cgagcagaag gcagccgatg agcgcgacgt agaggcgctg acgcctgagg
     5101 agctggacat cgtcgccgct gagatcttcg agcactaccg gatcgcggca tacatcctgg
     5161 cgtggacgag cctccggttc ggagagctga tcgagcttcg ccgcaaggac atcgtggacg
     5221 acggcatgac gatgaagctc cgggtgcgcc gtggcgcttc ccgcgtgggg aacaagatcg
     5281 tcgttggcaa cgccaagacc gtccggtcga agcgtcctgt gacggttccg cctcacgtcg
     5341 cggagatgat ccgagcgcac atgaaggacc gtacg
//
