LOCUS       sequence-345677-        5750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 5750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0913:Dendra
FEATURES             Location/Qualifiers
     source          1..5750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     720..739
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        720..738
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2368..2391
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2648..3448
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      3573..4161
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4062..4081
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcattcctgg tgaccacgac gcagggcaac cggctgtcga agtccgcgtt caccaagtcg
       61 ctgaagcgtg gctacgccaa gatcggtcgg ccggaactcc gcatccacga cctccgcgct
      121 gtcggcgcta cgttcgccgc tcaggcaggt gcgacgacca aggagctgat ggcccgtctc
      181 ggtcacacga ctcctaggat ggcgatgaag taccagatgg cgtctgaggc ccgcgacgag
      241 gctatcgctg aggcgatgtc caagctggcc aagacctcct gaaacgcaaa aagcccccct
      301 cccaaggaca ctgagtccta aagagggggg tttcttgtca gtacgcgaag aaccacgcct
      361 ggccgcgagc gccagcaccg ccgctctgtg cggagacctg ggcaccagcc ccgccgccgc
      421 caggagcatt gccgttcccg ccagaaatct agacggtgac cacaacgcgc ccgctttgat
      481 cggggacgtc gaaatattgg atcgtcggca ccgtcacggc cgtgggaggc ggcacgatcc
      541 gcgacgtgat gatcggccgc atccccacgg tgctgcgcag tgagctctac gccatcccgg
      601 cgttgatctg tgcgttcgca cgcacaggcc cggtgtgaga agggtctctg acgagcggga
      661 gaactcccta tcagtgatag agtttgtcct ccctatcagt gatagatagg cttaaggagt
      721 aatacgactc actataggga gaaggaggcc attatgtcaa acgtcgagtc cgaactggcg
      781 ccgtactacg aggaatcgca gtcgatctac gatatttcga acgaattctt cgcgctgttc
      841 ctcggcccca ccatgggcta cacctgcggt tactacgagc gcgaggacat gacgctcgac
      901 gagtcgcaga acgccaagtt cgatctcgca ctcggcaagc tggacctcaa gccgggcatg
      961 accctgctcg atgtcggctg cggctggggc ggcgcgctgg agcgggccgt caccaagtac
     1021 gacgtgaacg tgatcggcat cacccttagc aaggcgcagt cggactacgc gcgtgagcgc
     1081 ctcgcgaaga tcgagaccaa ccgcagcgtg gaagtccgcc tgcagggttg ggaagagttc
     1141 aacgagcccg tcgaccgcat cgtgtcgatc ggcgcgttcg aggccttcaa ggccgagcgc
     1201 tacccggtgt tcttcgagcg tgcgtacagc atcctgcccg acgacggccg catgctgctg
     1261 cacacgatcc tggcgcacac ccagaagttc ttccgcgaaa acggcatcaa gctgacgatc
     1321 agcgacctga agttcatgaa gttcatcggc gaggagatct tcccgggcgg tcagcttccc
     1381 gcggtcgagg acatcgagaa gctcgccgcg gactcgggct tcaagctcga gcgcatccac
     1441 ctgctgcagc ctcactacgc ccgcacgctc gacatgtggg cggccaacct cgaggccaag
     1501 aaggacgagg ccatcgccat gcagggccaa gaggtctacg accgctacat gaagtacctc
     1561 accggctgcg ccgacttctt ccgtcgcggc atcaccaacg tcggccagtt cacgctggtc
     1621 aagaagcttg gtgcggccgc gggcaacacg cctggcatca atctgatcaa ggaggacatg
     1681 cgtgtcaagg tgcatatgga gggcaacgtg aacggtcacg ccttcgtgat cgagggggag
     1741 ggtaagggta agccttacga agggacccag accgccaacc tcaccgtcaa ggagggcgcc
     1801 cctctcccat tctcgtacga tatcctcact accgcggtgc attacgggaa ccgcgtgttc
     1861 accaagtacc ccgaggatat ccctgactac ttcaagcaga gcttcccgga gggatactcc
     1921 tgggagcgga cgatgacctt cgaagacaag ggtatctgta cgatccgcag cgacatctcg
     1981 ctggaagggg actgtttctt ccagaacgtc cgtttcaagg ggaccaactt tccgccgaac
     2041 ggcccggtca tgcagaagaa gaccctcaag tgggagccga gcacggagaa gctgcacgtt
     2101 cgggacggcc tcctggtcgg caacatcaac atggcgctgc tgctggaagg cggcggccac
     2161 tacctatgcg acttcaagac cacgtacaaa gcgaagaagg tcgtccagct gcccgacgcg
     2221 cacttcgtgg accaccgcat cgaaatcctg gggaacgatt cggactacaa caaggtcaag
     2281 ttgtacgagc acgctgtggc acgctatagc ccgctcccgt cgcaggtctg gttcgaaggc
     2341 ggcggcgggg agaacctgta tttccaggac tacaaggacg acgacgacaa gtgatttaaa
     2401 taaccccttg gggcctctaa acgggtcttg aggggttttt tgttaactag cgtacgatcg
     2461 aattcctgca gctcacggta actgatgccg tatttgcagt accagcgtac ggcccacaga
     2521 atgatgtcac gctgaaaatg ccggcctttg aatgggttca tgtgcagctc catcagcaaa
     2581 aggggatgat aagtttatca ccaccgacta tttgcaacag tgccgttgat cgtgctatga
     2641 tcgactgatg tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc gaaaagccga
     2701 gctcatcggt cagcttctca accttggggt tacccccggc ggtgtgctgc tggtccacag
     2761 ctccttccgt agcgtccggc ccctcgaaga tgggccactt ggactgatcg aggccctgcg
     2821 tgctacgctg ggtccgggag ggacgctcgt catgccctcg tggtcaggtc tggacgacga
     2881 gccgttcgat cctgccacgt cgcccgttac accggacctt ggagttgtct ctgacacatt
     2941 ctggcgcctg ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg cagcggggcc
     3001 acaggcagag cagatcatct ctgatccatt gcccctgcca ccttactcgc ctgcaagccc
     3061 ggtcgcccgt gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg gacacgatgc
     3121 caacacgacg ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg tgccgagaca
     3181 ctgcaccatt cttcaggatg gcaagttggt acgcgtcgat tatctcgaga atgaccactg
     3241 ctgtgagcgc tttgccttgg cggacaggtg gctcaaggag aagagccttc agaaggaagg
     3301 tccagtcggt catgcctttg ctcggttgat ccgctcccgc gacattgtgg cgacagccct
     3361 gggtcaactg ggccgagatc cgttgatctt cctgcatccg ccagaggcgg gatgcgaaga
     3421 atgcgatgcc gctcgccagt cgattggctg agctcatgag cggagaacga gatgacgttg
     3481 gaggggcaag gtcgcgctga ttgctggggc aacacggggg atccactagt tccatgagcg
     3541 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3601 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3661 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     3721 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3781 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3901 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3961 gagctatgag aaagcgccac gcttcccgag gggagaaagg cggacaggta tccggtaagc
     4021 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4081 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4141 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4201 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4261 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcaacgcg
     4321 tgcggccgcg gtacccgggg atcctctaga gtcgaccacc aagggcacca tctctgcttg
     4381 ggccaccccg ttggccgcag ccagctcgct gagagccgtg aacgacaggg cgaacgccag
     4441 cccgccgacg gcgagggttc cgaccgctgc aactcccggt gcaaccttgt cccggtctat
     4501 tctcttcact gcaccagctc caatctggtg tgaatgcccc tcgtctgttc gcgcaggcgg
     4561 ggggctctat tcgtttgtca gcatcgaaag tagccagatc agggatgcgt tgcaaccgcg
     4621 tatgcccagg tcagaagagt cgcacaagag ttgcagaccc ctggaaagaa aaatggccag
     4681 agggcgaaaa caccctctga ccagcggagc gggcgacggg aatcgaaccc gcgtagctag
     4741 tttggaagaa tgggtgtctg ccgaccacat atgggccggt caagataggt ttttaccccc
     4801 tctcggctgc atcctctaag tggaaagaaa ttgcaggtcg tagaagcgcg ttgaagcctg
     4861 agagttgcac aggagttgca acccggtagc cttgttcacg acgagaggag acctagttgg
     4921 cacgtcgcgg atggggatcg ctgaagactc agcgcagcgg gaggatccaa gcctcatacg
     4981 tcaacccgca ggacggtgtg aggtactacg cgctgcagac ctacgacaac aagatggacg
     5041 ccgaagcctg gctcgcgggc gagaagcggc tcatcgagat ggagacctgg acccctccac
     5101 aggaccgggc gaagaaggca gccgccagcg ccatcacgct ggaggagtac acccggaagt
     5161 ggctcgtgga gcgcgacctc gcagacggca ccagggatct gtacagcggg cacgcggagc
     5221 gccgcatcta cccggtgcta ggtgaagtgg cggtcacaga gatgacgcca gctctggtgc
     5281 gtgcgtggtg ggccgggatg ggtaggaagc acccgactgc ccgccggcat gcctacaacg
     5341 tcctccgggc ggtgatgaac acagcggtcg aggacaagct gatcgcagag aacccgtgcc
     5401 ggatcgagca gaaggcagcc gatgagcgcg acgtagaggc gctgacgcct gaggagctgg
     5461 acatcgtcgc cgctgagatc ttcgagcact accggatcgc ggcatacatc ctggcgtgga
     5521 cgagcctccg gttcggagag ctgatcgagc ttcgccgcaa ggacatcgtg gacgacggca
     5581 tgacgatgaa gctccgggtg cgccgtggcg cttcccgcgt ggggaacaag atcgtcgttg
     5641 gcaacgccaa gaccgtccgg tcgaagcgtc ctgtgacggt tccgcctcac gtcgcggaga
     5701 tgatccgagc gcacatgaag gaccgtacga agatgaacaa gggccccgag
//
