LOCUS       sequence-345679-        6584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term fluorescent fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Judd JA, Canestrari J, Clark R, Joseph A, Lapierre P, 
            Lasek-Nesselquist E, Mir M, Palumbo M, Smith C, Stone M, Upadhyay A,
            Wirth SE, Dedrick RM, Meier CG, Russell DA, Dills A, Dove E, Kester 
            J, Wolf ID, Zhu J, Rubin ER, Fortune S, Hatfull GF, Gray TA, Wade 
            JT, Derbyshire KM
  TITLE     A Mycobacterial Systems Resource for the Research Community.
  JOURNAL   mBio. 2021 Mar 2;12(2). pii: mBio.02401-20. doi: 
            10.1128/mBio.02401-20.
  PUBMED    33653882
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-2"; volume: "12(2). pii: mBio.02401-20. doi"; pages: 
            " 10.1128/mBio.02401-20"
COMMENT     Sequence Label: pMSR:MSMEG_0866:Dendra
FEATURES             Location/Qualifiers
     source          1..6584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     341..360
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        341..359
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2823..2846
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3103..3903
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRATLGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPYSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      4028..4616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4517..4536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccgctctgt gcggagacct gggcaccagc cccgccgccg ccaggagcat tgccgttccc
       61 gccagaaatc tagacggtga ccacaacgcg cccgctttga tcggggacgt cgaaatattg
      121 gatcgtcggc accgtcacgg ccgtgggagg cggcacgatc cgcgacgtga tgatcggccg
      181 catccccacg gtgctgcgca gtgagctcta cgccatcccg gcgttgatct gtgcgttcgc
      241 acgcacaggc ccggtgtgag aagggtctct gacgagcggg agaactccct atcagtgata
      301 gagtttgtcc tccctatcag tgatagatag gcttaaggag taatacgact cactataggg
      361 agaaggaggc cattatgcgg gctgactcgg cgcccagcac ccaggcattg cggggctggc
      421 agcgcaaggc gctggtgaag tatctgacca ccaagccgcg tgattatctc ctggtcgcga
      481 cccctggtgc cggtaagacg acgtttgcgc tgcgcatcgc cgcggaactg ctcgccgacc
      541 gcaccgtgga caagatcacc gtcgtcgttc ccaccgagca cctcaagatc cagtgggcgg
      601 cctcggcagc gcgcaacggc atctcgctgg acccgaagtt cagcaactcg aactcgcaga
      661 cctcgtcgga gtacgacggc gtggtggtca cctacgccca ggtcgcgagc catccgacgc
      721 gccaccgggt gcgcaccgag aactaccgga cgctggtgat cttcgacgag atccaccacg
      781 gcggtgacgc caagagctgg ggcgacgcga tgcgggaggc ctacagcgac gccacccgcc
      841 gcctggccct gaccggtacc ccgttccgca gcgacgacag ccccatcccg ttcgtcaact
      901 acgaaccgga cgagagcggc ttcatgcggt cccaggccga ccatgtgtac ggttacgccg
      961 acgccctcgc cgacggcgtg gtgcggcccg tggtgttcct ggcgtactcg ggtgaggccc
     1021 gctggcgtga cagcgccggc gaggaacacg cggcgcgtct gggcgagccg ctgaccgccg
     1081 agcaaaccgc acgcgcctgg cgcaccgcgc tcaacccggc gggcgagtgg atgcccgcgg
     1141 tcatcaaggc cgccgaccgg cggttggagc agaagcgtca gcatgtgccc gacgcgggcg
     1201 gcatgatcat cgcgaccaac cagaccacgg cgcgcgccta cgcggacctg ctcaccaaga
     1261 tcaccggtga gacgcccacg gtcgtgctct cggacgaccc cagcgcctcc gaccgcatca
     1321 gccagtactc cgagtcgaac agcaagtggc tggtcgcggt gcgcatggtg tccgagggcg
     1381 tcgacgtgcc gcgcctgtcg gtcggcgtgt acgccacgag cgcgtcgaca ccgctgttct
     1441 tcgcacaggc catcggacgc ttcgtgcggt cgcggcgcca gggtgagacc gcgagcatct
     1501 tcctgccgtc ggtgccgaac ctgttgctgc tcgcctccga gatggaagcc cagcgcaacc
     1561 acgtgctggg caagccgcac cgcgagtccg acggactcga cgacgcggca ctcgaagagg
     1621 ccgagaaacg caaggacgag aagagcgagc tcgagaacgg gttcgaatac ctcggtgccg
     1681 acgccgaatt ggatcaggtc atcttcgacg gtgcctcgtg gggcaccgcg acaccggccg
     1741 gcagcgacga ggaggccgat tatctcggca tccccggcct gctcgacgcc gagcagatgc
     1801 gggacctgct gcgccgccgt caggaggaac agctcaccaa gcgcacggcc gtcgccgcgg
     1861 agaccggcgc tcccccgccg ccgcgcacca cgcacggcca attgcgcgag ctgcgaaagg
     1921 aactcaacgc gctggtgacc atcgcgcacc accgcacggg caagccgcac ggctggatcc
     1981 acaacgagtt gcggcgcatc tgcggtgggc cgccggtggc cgcggccacc accgagcagc
     2041 tcaaggagcg catcgaggcg gtccgcaggc tcaaccccaa gcttggtgcg gccgcgggca
     2101 acacgcctgg catcaatctg atcaaggagg acatgcgtgt caaggtgcat atggagggca
     2161 acgtgaacgg tcacgccttc gtgatcgagg gggagggtaa gggtaagcct tacgaaggga
     2221 cccagaccgc caacctcacc gtcaaggagg gcgcccctct cccattctcg tacgatatcc
     2281 tcactaccgc ggtgcattac gggaaccgcg tgttcaccaa gtaccccgag gatatccctg
     2341 actacttcaa gcagagcttc ccggagggat actcctggga gcggacgatg accttcgaag
     2401 acaagggtat ctgtacgatc cgcagcgaca tctcgctgga aggggactgt ttcttccaga
     2461 acgtccgttt caaggggacc aactttccgc cgaacggccc ggtcatgcag aagaagaccc
     2521 tcaagtggga gccgagcacg gagaagctgc acgttcggga cggcctcctg gtcggcaaca
     2581 tcaacatggc gctgctgctg gaaggcggcg gccactacct atgcgacttc aagaccacgt
     2641 acaaagcgaa gaaggtcgtc cagctgcccg acgcgcactt cgtggaccac cgcatcgaaa
     2701 tcctggggaa cgattcggac tacaacaagg tcaagttgta cgagcacgct gtggcacgct
     2761 atagcccgct cccgtcgcag gtctggttcg aaggcggcgg cggggagaac ctgtatttcc
     2821 aggactacaa ggacgacgac gacaagtgat ttaaataacc ccttggggcc tctaaacggg
     2881 tcttgagggg ttttttgtta actagcgtac gatcgaattc ctgcagctca cggtaactga
     2941 tgccgtattt gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc
     3001 ctttgaatgg gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcaccacc
     3061 gactatttgc aacagtgccg ttgatcgtgc tatgatcgac tgatgtcatc agcggtggag
     3121 tgcaatgtcg tgcaatacga atggcgaaaa gccgagctca tcggtcagct tctcaacctt
     3181 ggggttaccc ccggcggtgt gctgctggtc cacagctcct tccgtagcgt ccggcccctc
     3241 gaagatgggc cacttggact gatcgaggcc ctgcgtgcta cgctgggtcc gggagggacg
     3301 ctcgtcatgc cctcgtggtc aggtctggac gacgagccgt tcgatcctgc cacgtcgccc
     3361 gttacaccgg accttggagt tgtctctgac acattctggc gcctgccaaa tgtaaagcgc
     3421 agcgcccatc catttgcctt tgcggcagcg gggccacagg cagagcagat catctctgat
     3481 ccattgcccc tgccacctta ctcgcctgca agcccggtcg cccgtgtcca tgaactcgat
     3541 gggcaggtac ttctcctcgg cgtgggacac gatgccaaca cgacgctgca tcttgccgag
     3601 ttgatggcaa aggttcccta tggggtgccg agacactgca ccattcttca ggatggcaag
     3661 ttggtacgcg tcgattatct cgagaatgac cactgctgtg agcgctttgc cttggcggac
     3721 aggtggctca aggagaagag ccttcagaag gaaggtccag tcggtcatgc ctttgctcgg
     3781 ttgatccgct cccgcgacat tgtggcgaca gccctgggtc aactgggccg agatccgttg
     3841 atcttcctgc atccgccaga ggcgggatgc gaagaatgcg atgccgctcg ccagtcgatt
     3901 ggctgagctc atgagcggag aacgagatga cgttggaggg gcaaggtcgc gctgattgct
     3961 ggggcaacac gggggatcca ctagttccat gagcgtcaga ccccgtagaa aagatcaaag
     4021 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4081 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4141 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     4201 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4261 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4321 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4381 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4441 ccgaggggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4501 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4561 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4621 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4681 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4741 ccgctcgccg cagccgaacg accgagcgca acgcgtgcgg ccgcggtacc cggggatcct
     4801 ctagagtcga ccaccaaggg caccatctct gcttgggcca ccccgttggc cgcagccagc
     4861 tcgctgagag ccgtgaacga cagggcgaac gccagcccgc cgacggcgag ggttccgacc
     4921 gctgcaactc ccggtgcaac cttgtcccgg tctattctct tcactgcacc agctccaatc
     4981 tggtgtgaat gcccctcgtc tgttcgcgca ggcggggggc tctattcgtt tgtcagcatc
     5041 gaaagtagcc agatcaggga tgcgttgcaa ccgcgtatgc ccaggtcaga agagtcgcac
     5101 aagagttgca gacccctgga aagaaaaatg gccagagggc gaaaacaccc tctgaccagc
     5161 ggagcgggcg acgggaatcg aacccgcgta gctagtttgg aagaatgggt gtctgccgac
     5221 cacatatggg ccggtcaaga taggttttta ccccctctcg gctgcatcct ctaagtggaa
     5281 agaaattgca ggtcgtagaa gcgcgttgaa gcctgagagt tgcacaggag ttgcaacccg
     5341 gtagccttgt tcacgacgag aggagaccta gttggcacgt cgcggatggg gatcgctgaa
     5401 gactcagcgc agcgggagga tccaagcctc atacgtcaac ccgcaggacg gtgtgaggta
     5461 ctacgcgctg cagacctacg acaacaagat ggacgccgaa gcctggctcg cgggcgagaa
     5521 gcggctcatc gagatggaga cctggacccc tccacaggac cgggcgaaga aggcagccgc
     5581 cagcgccatc acgctggagg agtacacccg gaagtggctc gtggagcgcg acctcgcaga
     5641 cggcaccagg gatctgtaca gcgggcacgc ggagcgccgc atctacccgg tgctaggtga
     5701 agtggcggtc acagagatga cgccagctct ggtgcgtgcg tggtgggccg ggatgggtag
     5761 gaagcacccg actgcccgcc ggcatgccta caacgtcctc cgggcggtga tgaacacagc
     5821 ggtcgaggac aagctgatcg cagagaaccc gtgccggatc gagcagaagg cagccgatga
     5881 gcgcgacgta gaggcgctga cgcctgagga gctggacatc gtcgccgctg agatcttcga
     5941 gcactaccgg atcgcggcat acatcctggc gtggacgagc ctccggttcg gagagctgat
     6001 cgagcttcgc cgcaaggaca tcgtggacga cggcatgacg atgaagctcc gggtgcgccg
     6061 tggcgcttcc cgcgtgggga acaagatcgt cgttggcaac gccaagaccg tccggtcgaa
     6121 gcgtcctgtg acggttccgc ctcacgtcgc ggagatgatc cgagcgcaca tgaaggaccg
     6181 tacgaagatg aacaagggcc ccgaggcatt cctggtgacc acgacgcagg gcaaccggct
     6241 gtcgaagtcc gcgttcacca agtcgctgaa gcgtggctac gccaagatcg gtcggccgga
     6301 actccgcatc cacgacctcc gcgctgtcgg cgctacgttc gccgctcagg caggtgcgac
     6361 gaccaaggag ctgatggccc gtctcggtca cacgactcct aggatggcga tgaagtacca
     6421 gatggcgtct gaggcccgcg acgaggctat cgctgaggcg atgtccaagc tggccaagac
     6481 ctcctgaaac gcaaaaagcc cccctcccaa ggacactgag tcctaaagag gggggtttct
     6541 tgtcagtacg cgaagaacca cgcctggccg cgagcgccag cacc
//
